Presentation given at SGM Metagenomics Hot-topic Session in Edinburgh, UK

1. Metagenomics and Metaproteomics of Polysaccharide-Degrading Bacteria Mike Cox, Jennifer Edwards, Clive Edwards and Alan McCarthy

3. Cellulose

4. Chitin

5. using an integrated ‘omic’ approach to examine the presence and diversity of polysaccharide-degrading microorganisms that colonise baits in situ .

6. Metagenomics Metaproteomics SIP Isolation Diversity

7. MetaprotogenomoSIPalomics

8. Screening Expression Probing

10. Probe design problems Protein Databases Modular Enzymes Large Numbers Diverse Domains Substrate Specificity

11. GH5 Family Architecture

13. Metaproteomics

18. 13 C Cellulose did not degrade 13 C – O bond 16-18 x stronger

19. Jennifer Edwards Clive Edwards Alan McCarthy Rob Lockhart Paul Loughnane James McDonald Alex de Menezes Lab H, University of Liverpool Aquatic Metagenomics Consortium Bela Tiwari NEBC NERC SGM