Bacteria Isolation and Characterization Presentation

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Camille Ruiz

Dr. Rubin's project

Science

ISOLATING BACTERIA FROM
TROPICAL CONTAMINATED
SOILS IN PUERTO RICO
RISE SPRING 2015
Sara Diaz
Patricia Rodriguez
Camille Ruiz
Justin Cotto

SAMPLING LOCATIONS
Sample Coordinates Environmental description Location Date/Time
Ruiz C
- #1
18°6’55.1”N;
66°9’36.2”W
Moist (underneath grass),
5 cm; 72°F
Cayey, PR January/28/15
7:30 p.m.
Cotto J
- #2
18.15º N
66.18ºW
Moist environment (above
grass), 76ºF
Cidra, PR January/29/15
8:00 a.m.

SAMPLING LOCATIONS
Sample Coordinates Environmental description Location Date/Time
Diaz S
-#1
18°15'15.7"N
66°06'06.9"W
Moist environment
80°F
Aguas
Buenas, P.R.
January/27/1
5
4:26 p.m.
Rodríguez P
-#2
18°15'15.7"N
66°06'06.9"W
Moist environment
80°F
Aguas
Buenas, P.R.
January/27/1
5
4:26 p.m.

RESULTS FROM DIFFERENT PROCEDURES
Process CLRV30TSA01A CLRV30M01B JCR30TSA01A
Gram Stain Negative Positive Positive
Bacterial Morphology Coccus Bacillus Bacillus
PCR/Electrophoresis #1 Negative Negative Negative
PCR/Electrophoresis #2 Positive Negative -
Antibiotic Production Negative Negative Negative

GRAM STAIN RESULTS
CLRV30TSA01A CLRV30M01B JCR30TSA01A

RESULTS FROM DIFFERENT PROCEDURES
Process S15UPRC-RISESCDM30T01B S15UPRC-RISEPR30M01
Gram Stain Positive Positive
Bacterial Morphology Bacillus Bacillus
PCR/Electrophoresis #1 Negative Negative
PCR/Electrophoresis #2 Negative Negative
Antibiotic Production Negative Negative

GRAM STAIN RESULTS
 S15UPRC-RISEPR30M01 S15UPRC-RISESCDM30T01B

ANTIBIOTIC RESISTANCETEST RESULTS
Antibiotic CLRV30TSA01A CLRV30M01B JCR30TSA01A
Kanamycin 2.0 cm 1.0 cm 2.0 cm
Penicillin 0.0 cm 0.0 cm -
Tetracycline 1.6 cm 2.4 cm 2.2 cm
Gentamicin 1.9 cm 0.9 cm -
Chloramphenicol 0.0 cm 0.0 cm 2.5 cm
Bacitracin 0.0 cm 0.0 cm 1.5 cm
Vancomycin 0.0 cm 0.0 cm -
Streptomycin 1.6 cm 1.0 cm 1.9 cm

ANTIBIOTIC RESISTANCETEST RESULTS
Antibiotic S15UPRC-RISESCDM30T01B
Streptomycin 2.2 cm
Chloramphenicol 2.3 cm
Erythromycin 1.0 cm
Kanamycin 1.6 cm
Gentamicin 0.6 cm
Antibiotic S15UPRC-RISEPR30M01
Rifampin 0.13 cm
Kanamycin 0.16 cm
Vancomycin 0.17cm
Streptomycin 0.30 cm
Tetracyclin 0.14 cm

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Bacteria Isolation and Characterization Presentation

1. ISOLATING BACTERIA FROM TROPICAL CONTAMINATED SOILS IN PUERTO RICO RISE SPRING 2015 Sara Diaz Patricia Rodriguez Camille Ruiz Justin Cotto

2. SAMPLING LOCATIONS Sample Coordinates Environmental description Location Date/Time Ruiz C - #1 18°6’55.1”N; 66°9’36.2”W Moist (underneath grass), 5 cm; 72°F Cayey, PR January/28/15 7:30 p.m. Cotto J - #2 18.15º N 66.18ºW Moist environment (above grass), 76ºF Cidra, PR January/29/15 8:00 a.m.

3. SAMPLING LOCATIONS Sample Coordinates Environmental description Location Date/Time Diaz S -#1 18°15'15.7"N 66°06'06.9"W Moist environment 80°F Aguas Buenas, P.R. January/27/1 5 4:26 p.m. Rodríguez P -#2 18°15'15.7"N 66°06'06.9"W Moist environment 80°F Aguas Buenas, P.R. January/27/1 5 4:26 p.m.

4. RESULTS FROM DIFFERENT PROCEDURES Process CLRV30TSA01A CLRV30M01B JCR30TSA01A Gram Stain Negative Positive Positive Bacterial Morphology Coccus Bacillus Bacillus PCR/Electrophoresis #1 Negative Negative Negative PCR/Electrophoresis #2 Positive Negative - Antibiotic Production Negative Negative Negative

5. GRAM STAIN RESULTS CLRV30TSA01A CLRV30M01B JCR30TSA01A

6. POSITIVE ELECTROPHORESIS

7. RESULTS FROM DIFFERENT PROCEDURES Process S15UPRC-RISESCDM30T01B S15UPRC-RISEPR30M01 Gram Stain Positive Positive Bacterial Morphology Bacillus Bacillus PCR/Electrophoresis #1 Negative Negative PCR/Electrophoresis #2 Negative Negative Antibiotic Production Negative Negative

8. GRAM STAIN RESULTS  S15UPRC-RISEPR30M01 S15UPRC-RISESCDM30T01B

9. ANTIBIOTIC RESISTANCETEST RESULTS Antibiotic CLRV30TSA01A CLRV30M01B JCR30TSA01A Kanamycin 2.0 cm 1.0 cm 2.0 cm Penicillin 0.0 cm 0.0 cm - Tetracycline 1.6 cm 2.4 cm 2.2 cm Gentamicin 1.9 cm 0.9 cm - Chloramphenicol 0.0 cm 0.0 cm 2.5 cm Bacitracin 0.0 cm 0.0 cm 1.5 cm Vancomycin 0.0 cm 0.0 cm - Streptomycin 1.6 cm 1.0 cm 1.9 cm

10.

11.

12. ANTIBIOTIC RESISTANCETEST RESULTS Antibiotic S15UPRC-RISESCDM30T01B Streptomycin 2.2 cm Chloramphenicol 2.3 cm Erythromycin 1.0 cm Kanamycin 1.6 cm Gentamicin 0.6 cm Antibiotic S15UPRC-RISEPR30M01 Rifampin 0.13 cm Kanamycin 0.16 cm Vancomycin 0.17cm Streptomycin 0.30 cm Tetracyclin 0.14 cm

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