This document summarizes a lecture on requirements engineering. It discusses defining functional and non-functional requirements, writing user and system requirements, and techniques for gathering requirements such as interviews and questionnaires. The key aspects of requirements engineering are establishing customer needs, analyzing and documenting system constraints and services, and checking requirements for validity, consistency and completeness.
Extracting Quality Scenarios from Functional ScenariosProf. Amir Tomer
Discovering quality attributes by applying "how well?" questions to functions in Use-Case structured scenarios, and then enriching the software architecture with additional functionality.
Extracting Quality Scenarios from Functional ScenariosProf. Amir Tomer
Discovering quality attributes by applying "how well?" questions to functions in Use-Case structured scenarios, and then enriching the software architecture with additional functionality.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
This pdf is about the Schizophrenia.
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Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
2. The requirements for a system are the descriptions of what the
system should do the services that it provides and the constraints on
its operation.
These requirements reflect the needs of customers for a system that
serves a certain purpose such as controlling a device, placing an
order, or finding information.
The process of finding out, analyzing, documenting and checking
these services and constraints is called requirements engineering
(RE).
Introduction
3. Requirements engineering
The process of establishing the services that a
customer requires from a system and the
constraints under which it operates and is
developed.
5. Requirements definition/specification
1. Requirement definition: A statement in natural language plus
diagrams of the services the system provides and its operational
constrains. Written for customers(User requirements).
2. Requirement specification: A structured document setting out
detailed descriptions of the system services, written as a contract
between customer and contractor(System requirements).
3. Software specification: A detailed software description which can
serve as a basis for a design or implementation. Written for
developers.
6. Requirements Checking
1. Validity: Does the system provide the functions which best support
the customer’s needs?
2. Consistency : Are there any requirements conflicts?
3. Completeness: Are all functions required by the customer included?
4. Realism: Can the requirements be implemented given the available
budget and technology?
8. Functional and non-functional
requirements
Functional requirements:
▪ Statements of services the system should provide, how the system should react to particular inputs
and how the system should behave in particular situations.
▪ May state what the system should not do.
Non-functional requirements:
▪ Constraints on the services or functions offered by the system such as timing constraints,
constraints on the development process, standards, etc.
▪ Often apply to the system as a whole rather than individual features or services.
Domain requirements
▪ Constraints on the system from the domain of operation
9. Functional requirements
Describe functionality or system services.
Depend on the type of software, expected users and the type of
system where the software is used.
Functional user requirements may be high-level statements of
what the system should do.
Functional system requirements should describe the system
services in detail.
10. Mentcare system: functional
requirements
A user shall be able to search the appointments lists for all
clinics.
The system shall generate each day, for each clinic, a list of
patients who are expected to attend appointments that day.
Each staff member using the system shall be uniquely identified
by his or her 8-digit employee number.
11. Non-functional requirements
These define system properties and constraints e.g. reliability, response time
and storage requirements. Constraints are I/O device capability, system
representations, etc.
Process requirements may also be specified mandating a particular IDE,
programming language or development method.
Non-functional requirements may be more critical than functional
requirements. If these are not met, the system may be useless.
13. Non-functional requirements
implementation
Non-functional requirements may affect the overall architecture of a
system rather than the individual components.
▪ For example, to ensure that performance requirements are met,
you may have to organize the system to minimize communications between
components.
A single non-functional requirement, such as a security requirement, may
generate a number of related functional requirements that define system
services that are required.
▪ It may also generate requirements that restrict existing requirements.
14. Non-functional classifications
Product requirements
▪ Requirements which specify that the delivered product must behave in a
particular way e.g. execution speed, reliability, etc.
Organisational requirements
▪ Requirements which are a consequence of organizational policies and
procedures e.g. process standards used, implementation requirements, etc.
External requirements
▪ Requirements which arise from factors which are external to the system
and its development process e.g. interoperability requirements, legislative
requirements, etc.
15. Examples of nonfunctional
requirements in the Mentcare system
Product requirement
The Mentcare system shall be available to all clinics during normal
working hours (Mon–Fri, 0830–17.30). Downtime within normal
working hours shall not exceed five seconds in any one day.
Organizational requirement
Users of the Mentcare system shall authenticate themselves using
their health authority identity card.
External requirement
The system shall implement patient privacy provisions as set out in
HStan-03-2006-priv.
16. The software requirements document
The software requirements document (sometimes called the software
requirements specification or SRS)
official statement of what the system developers should implement.
It should include both the user requirements for a system and a detailed
specification of the system requirements.
17.
18. Requirements specification
Requirements specification is the process of writing down the user and
system requirements in a requirements document.
the user and system requirements should be:
1.clear,
2.unambiguous,
3.easy to understand,
4.complete,
5.and consistent
19. Requirement Notes
1. The user requirements for a system should describe the functional
and nonfunctional requirements.
2. The requirements document should not include details of the system
architecture or design.
3. the system requirements should simply describe the external
behavior of the system and its operational constraints.
4. User requirements are almost always written in natural language
supplemented by appropriate diagrams and tables in the
requirements document.
5. Graphical models are most useful when you need to show how a state
changes or when you need to describe a sequence of actions.
20. Requirements engineering processes
The processes used for RE vary widely depending on the application domain,
the people involved and the organization developing the requirements.
However, there are a number of generic activities common to all processes
▪ Requirements elicitation;
▪ Requirements analysis;
▪ Requirements validation;
▪ Requirements management.
In practice, RE is an iterative activity in which these processes are interleaved.