This document discusses DNA extraction from cheek cells. It explains that all human cells contain the same DNA organized into chromosomes. While cheek cells and liver cells contain identical DNA, different genes are turned on or off in each cell type. The document then describes the steps involved in extracting DNA from cheek cells, including collecting cells, dissolving cell membranes, breaking down proteins, and precipitating the DNA.

1. SBT 3013BIOTECHNOLOGY TITLE: CHEEK CELL DNA EXTRACTION

3. DNA we get is determined from the egg and sperm that combined to make us.

6. For 23 chromosomes, there are over 10,000,000,000,000 possibilities*

10. Transcription and Translation An intermediate, mRNA is needed to copy the information from the genomic DNA to traslated into proteins.

11. Why intermediate (mRNA) is needed? The order of amino acids in polypeptide is determined by the sequence of 3-letteer codes in mRNA.

12. RESULT

14. Types of other molecules:

15. ATP

16. RNA

17. amino acids

18. proteins including enzymes lipids

19. carbohydrates including sugars

20. Agent used to break up unwanted:

21. Soap or detergent

23. It is doubtful that a protease that works best at 50˚C would be found in a bacterium that lives at typical body temperature.

25. Meat is often tenderized before cooking, to make it less tough and more suitable for consumption. Meat tenderizers often come in a powdered form that can be sprinkled directly on the meat. Meat tenderizers dissolve some of the sinewy connections within the meat at room temperature. If meat tenderizers are allowed to act for too long, the meat can become squishy and lose its special texture.

27. CONCLUSION Collect the cells Dissolve cell membrane Break down proteins Precipitate the DNA Make DNA less soluble in water

28. Reference http://faculty.clintoncc.suny.edu/faculty/michael.gregory/files/bio%20101/bio%20101%20lectures/protein%20synthesis/protein.htm