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Dna extraction review
- 1. Christiane’s Super CrazyComplicated Difficult Challenging Super Crazy Professional Guide to DNA Extraction
- 2. Christiane’s Guide toDNA Extraction 1. 2. 3. 4. Spit in a cup Add a drop of soap Add a pinch of salt and mix Add three spoonfuls of alcohol
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- 19. Christiane’s Guide toDNA Extraction • Spit in a cup (~1mL of saliva) • Add a drop of soap and mix You do not need a lot • Add a pinch of salt and mix • Add three spoonfuls of alcohol – Cover the top of the container – Swirl the cup, but DO NOT mix the contents with a spoon, otherwise you will break up the DNA into fragments – Allow for the contents to settle – If DNA is not visible after a few minutes, add another spoonful of alcohol
Editor's Notes
- #7 The DNA is inside of the cells in your cheeks which are constantly being moved around by saliva.
- #8 Step 2: SoapBreak open the cells the saliva contains. The detergents in the dish soap destabilize the membranes of the cells, spilling their contents into the rest of the solution of saliva. This includes all of the proteins, sugars, and DNA.However, all of this stuff is still dissolved in the saliva and can't see the DNA yet. DNA is free but still dissolved in solution.
- #15 Step 3: SaltDNA loves water!Adding salt to the solution will interact To get the DNA to precipitate and solidify, we need to do something about each DNA molecule's negatively-charged phosphate backbone. When we dissolve the table salt in the solution, some of the positively-charged sodium ions will interact with the negatively-charged regions of the DNA molecules and effectively shield other nearby DNA molecules from their repulsive force - this will help them all aggregate and clump together in the next step. To visualize the idea here, imagine the resistance you feel when you begin to push the south poles of two magnets together - this is sort of like what's going on between the individual DNA molecules. Now imagine inserting the north pole of a third magnet between the south poles of the first two - the resistance is reduced. The north pole of the third magnet is sort of like the sodium ion in our solution
- #19 Step 4: AlcoholDNA is not soluble in alcohol however, so the addition of alcohol will cause the DNA to come out of solution and appear as a solid. You should see some cloudy, snot-like white stuff suddenly appearing. This is your DNA precipitating out of solution!