Mr. G. Santhosh Kumar is a research associate who has developed real-time PCR diagnostic kits for diseases like malaria, tuberculosis, hepatitis B, dengue, rabies, HIV, and hepatitis C. He has experience designing primers and probes for infectious diseases, isolating nucleic acids, and standardizing real-time PCR assays. Previously, he purified proteins including streptolysin O, insulin, and interleukin-2.

1. Mr. G. Santhosh Kumar
House no: 149, 6th cross, KEB layout
Geddalahalli, Sanjaynagar, Bangalore.
Mobile: +91-9342296454, 9019724796
e-mail: santhoshmbt@gmail.com
Present Position:
Research Associate (Since May 2009 to date)
Achievement:
 A METHOD FOR ISOLATION OF NUCLEIC ACIDS AND A KIT THEREOF
United States Patent Application –PCT/IB2011/050044, WO/2011/083429
Inventors: Phani Kumar Pullela, Manoj MN, Santhosh Kumar G, Pinto MP, Chandrasekhar
Nair B, Subba Rao PV.
 Santhosh Kumar G, Manjula J, Rajasekaran C, Manoj MN, Subbarao PV, Chandrasekhar B
Nair. TRENDS IN THE DIAGNOSIS OF HUMAN IMMUNODEFICIENCY VIRUS, Review, Asian
journal of pharmaceutical and clinical research, Vol 6, Suppl 4, 2013.
Present work:
Development of real time PCR based diagnostic kits
 Isolation of DNA and RNA – (from blood, plasma and sputum) using standard kits (spin
column based) and in-house method (silica coated iron nano particles based).
 Design and development of real time PCR based diagnostic assays on Applied Biosystems
real time PCR and in-house fabricated chip based real time micro PCR device.
 Developed qPCR kits for malaria, tuberculosis and hepatitis B.
 Developed of Reverse Transcription qPCR for the diagnosis of Chikungunya, Dengue,
Rabies, HIV and Hepatitis C virus.
 Melt curve analysis for the detection rifampicin resistance in Mycobacterium tuberculosis
and differentiation of HCV genotypes.
 Standardization and immobilization of PCR master mix buffers.
 Analysis of PCR product by agarose gel electrophoresis and melt curve.

2. Bioinformatics
 Hands on experience in designing primers and Taqman probe for infectious disease.
 Designed internal positive control for real time PCR.
 Hands on experience in epitope prediction to develop immunoassay kits for infectious
diseases.
Cloning
 Cloning of PCR products using TA cloning kit for positive control.
 Prepared RNA transcripts for developing standard curve.
Detection of small molecules (Proteins and peptides)
 Purification of insulin from yeast culture medium.
 Purification of Recombinant Protein IL-2.
 Purification of antibodies from serum and cell culture medium using affinity, ion exchange
and gel permeation chromatography.
 Coupling of small proteins and peptides to BSA or KLH for immunization.
 Production of polyclonal antibodies against peptides and proteins. Coupling of peptides,
antigens to CNBr Sepharose for purification of antibodies.
 ELISA for acute kidney injury markers like NGAl and Cystatin C.
 Double immuno diffusion, electrophoresis, radial immuno diffusion, SDS PAGE and western
blot assay.
Collaborative Work
 National Institute of Virology (NIV) – Assay development on real time PCR assay for
screening/detection of Swine flu, Chikungunya.
 Kasturibha medical college, Mangalore – Diagnosis of HIV using rotor gene qPCR.
 Defense Research Development and Establishment (DRDE) – Development of chip based
real time micro PCR based assay for Dengue and Malaria as part of technology transfer.
Previous project:

3. Mediclone Biotech, Chennai (August 2008 to April 2009)
Purification of Streptolysin O from Streptococcus pyogenes using Ion exchange, gel
permeation, HIC and Affinity chromatography.
Academic Profile:
2006 – 08 M.Tech. (Medical Biotechnology)
University of Hyderabad, Hyderabad
2003 – 05 M.Sc. (Biochemistry)
GITAM, Affiliated to Andhra University, Visakhapatnam
2000 –03 B.Sc. (Biochemistry, Microbiology, Chemistry)
SSN Degree College, Affiliated to Nagarjuna University
 M.Tech Project : Protective effect of plant extract against alloxan induced diabetes in rats
 M.Sc. Dissertation : Radio Immuno Assay
 Qualified GATE in 2006 Life Sciences Branch.
 Qualified state eligibility test for Assistant professor/lectureship in 2012
Areas of Interest:
 Protein Work
Down Stream processing, Purification and Characterization.
 Molecular Biology
Cloning, Screening, Gene Expression.
 Diagnostics
Immunoassays and Real-time PCR (DNA/RNA) based kit development.
Personal Profile:
DOB: 14 / 09 / 1982.
Languages Known: English, Hindi, Telugu, Kannada and Tamil.

4. Marital Status: Married.