Sandeep Kumar is a PhD student at the Centre for Human Genetics and Molecular Medicine at Central University of Punjab in Bathinda, India. He received his MSc in Microbiology from Bundelkhand University in Bhopal, India. His research experience includes over 3 years as an SRF studying bacterial pathogens. He has published 4 papers and attended numerous workshops and courses on topics like immunology, proteomics, and structural biology. He has skills in techniques like PCR, cloning, protein purification, and bioinformatics.

1. SANDEEP KUMAR
PhD. Student
Centre for Human Genetics and Molecular Medicine
Central University of Punjab
Bathinda-151001
Father Name: Mr. BIRI SINGH
Mother Name: Mrs. JANAKI DEVI
D/O/B: 13/01/1985
Permanent Address:
Vill. Sakrain, P.O. Tenaher, Teh. Sarkaghat, Distt. Mandi, H.P.-175050
Mob. No. : +919418713764
e_mail: sandeep.kumar665@gmail.com
Educational Qualification:
S. No. Class/
Degree
Subjects/Discipline Marks
Obtained
Percentage
(%)
Board/
University
1. 10th
Eng., Math, Hindi, S. Sci.,
Sci., Sansk., Art
405/700 57.85 HPSB
Dharamsahla
2. 12th
English, Physics,
Chemistry, Biology & IT
255/500 55.00 do-
3. B.Sc. Chemistry, Zoology,
Botany, English &Hindi
1014/2000 50.70 HPU, Shimla
4. M.Sc. Microbiology 866/1200 72.16 BU, Bhopal
M. Sc. Dissertation:
”Assessment of Genetic Diversity of Pseudomonas spp. through RAPD Markers”.
PI: Dr. N.N. Malhotra
Laboratory: Centre for Excellence in Biotechnology
Institute: Madhya Pradesh Counsel of Science & Technology, Bhopal, M.P.
Research Experience:
S.No. Position Experience Research Project Name PI Laboratory/Institute/
University
1. SRF 3 years, 10
months and
28 days
“All India Network Programme on
Haemorrhagic Septicemia”
Dr.
Mandeep
Sharma
Department of Veterinary
Microbiology,
DGCNCOVAS, CSKHPKV,
Palampur, H.P.-176062
2. SRF 1 year and 8
days
“A study of serotypic differences in
innate and adaptive immune
responses in Shigellosis in humans”
Dr. Neelam
Taneja
Enteric Lab., Department
of Medical Microbiology,
PGIMER, Research Block
A, Chandigarh, India-
160012
3. SRF 9 months "Isolation, 3D structural
characterization and functional
analysis of lectins from microbial
sources"
Dr. Desh
Deepak
Singh
Centre for Genetic
Diseases and Molecular
Medicine, Central
University of Punjab,
Bathinda, Punjab-151001

2. Research Techniques Learned:
 Culture, isolation, identification and CST of bacterial pathogens from animals and human
clinical samples.
 Preservation of bacterial isolates by glycerol stock and lyophilisation.
 AGPT, RPBT, SAT and indirect ELISA.
 Isolation of DNA (genomic/plasmid) from bacteria, fungus and tissues etc. by
phenol:chloroform:isoamyl alcohol and from kits.
 Isolation of RNA from bacteria, blood and tissues by RNasy kit and Trizol methods.
 Quantification of DNA, RNA and protein samples by Nanodrop/Biodrop spectrophotometers.
 PCR (gene specific, RAPD, ERIC, nested and multiplex).
 cDNA preparation from RNA samples for qRT-PCR analysis.
 Transformation of bacterial knockout plasmid by electroporation and heat shock methods
and their screening.
 Preparation of blood samples for flow cytometery (FACS-Caliber) to analyse CD4, CD8, CD21
and WLC.
 Purification of PCR products and sending them for sequencing, sequence editing and analysis
by Bio-Edit and NCBI and Expasy tools.
 Human colon biopsy culture and immunohistochemical staining for confocal microscopy
 Biostatical analysis of research data by MS-Excel and SPSS.
 Cloning and expression of gene
 Isolation of Protein by ammonium sulphate precipitation, affinity, ion-exchange and size
exclusion chromatography.
 Quantification of protein concentration using Bradford assay and 280 nm.
 SDS-PAGE and Biofilm-assay
Computer Skills:
 Report, presentation, poster and research paper writing in MS-Office
 Data analysis using MS-Excel and SPSS.
 Data mining by different data search engines.
Publications: 4
1. Profiling of virulence associated genes of Pasteurella multocida isolated from cattle.
S Verma, M Sharma, S Katoch, L Verma, S Kumar, V Dogra, R Chahota and G Singh Veterinary
research communications 37 (1), 2013, 83-89
2. In vitro and in vivo pathogenicity studies of Pasteurella multocida strains harbouring
different ompA
S Katoch, M Sharma, RD Patil, S Kumar, S Verma Veterinary research communications, 38 (3),
2014 183-191
3. Experimental Study of the Pathogenicity of Pasteurella multocida Capsular Type B in
Rabbits
S. Katoch, L. Verma, M. Sharma, R. K. Asrani, S. Kumar, R. Chahota and S. Verma Journal of
Comparative Pathology 153, 2–3, 2015, 160–166
4. Bacterological, molecular and antibiotic sensitivity profile of Pasteurella multocida from
bovine and rabbits
Lovit Verma, Mandeep Sharma, Shailja Katoch, Vishal Dogra, Sandeep Kumar, Prasenjit
Dhar, Rajesh Chahota and Subhash Verma Indian Veterinary Journal 92 (3), 2015 38-41
Workshops/Courses Attended: 8
1. International Workshop on “Transcriptomics, Proteomics and Structural Biology” 27th
to
29th
Feb., 12. Animal Biotechnology Centre, NDRI, Karnal, Haryana, India.

3. 2. “Identification and Molecular Characterization of Dichelobacter nodusus Causative Agent
of Foot Root”. 14th
to 20th
Dec., 2009. Department of Veterinary Microbiology, DGCNCOVAS,
CSKHPKV, Palampur, H.P.-176062
3. National Workshop on “Cytokine Analysis and Phenotyping of Breast Tumor Cells Using
Flow Cytometer” 21st
to 22nd
January 2016, CHGMM, CUP, Bathinda, India-151001
4. FIMSA “13th
Advance Immunology Course” 17th
to 19th
March 2016, Department of
Immunopathology, PGIMER, Chandigarh, India
5. GIAN Course on “Molecular Underpinning of Secretion and Membrane Fusion” 19th
to 25th
June 2016, SLS, JNU, New Delhi, India
6. GIAN Course on “Immunologicals in Animal and Human Health” 4th
to 16th
July 2016, SLS,
Hyderabad University, Hyderabad, India
7. NPTEL Online Certification Course on “Protein and Gel-Based Proteomics” Sep. to Oct.,
2015, IIT Bombay, India
8. NPTEL Online Certification Course on “Mass Spectroscopy Based Proteomics” Sep. to Oct.,
2015, IIT Bombay, India
Conferences/Seminars/Symposium Attended: 8
 Life member of Indian Science Congress Association (L23380)
 Student member of Indian Crystallographic Association (SM 203)