Jeffrey H. Stanwix is seeking a position in disease pathology research applying his passion for human biology. He has a Bachelor's degree in Biology from Northeastern University where he gained experience with various lab techniques including DNA sequencing, cell culture, and protein analysis. His professional experience includes research roles at Pfizer, Genzyme, and Woods Hole Oceanographic Institution where he conducted protein analysis, optimized antibody use, developed cell testing methods, and assisted with phytoplankton sampling and analysis related to red tide toxicity.

1. Jeffrey
H.
Stanwix
Email:
jeffrey.h.stanwix@gmail.com
Phone:
860-­‐395-­‐8081
Objective: Apply my passion for understanding human biology to a challenging position in disease pathology research.
EDUCATION
Northeastern University, Boston, MA
Bachelor of Science in Biology, May 2015
Relevant Coursework: Ecology, Physics, Organic Chemistry, Genetics and Molecular Biology, Psychobiology, Behavioral Endocrinology, Cell
Biology, Biochemistry, Neurobiology
Academic Awards: Academic Scholarship, Dean’s List, Northeastern University Honors College Student
SKILLS & TECHNIQUES
DNA Preparation and Sequencing, SDS-PAGE, Western Blots, PCR, Agarose Gel Preparation, Gel Electrophoresis, Sequencing Reactions,
mRNA Sample Preparation and Quantitation, iScript cDNA Preparation, Cell Culture Maintenance and Transfection
Additional Skills: Fluorescent In Situ Whole Cell Hybridization, Growing and Plating Bacteria Cultures, Experience with Field Biology,
Sterile Techniques, Dissection, Experience Operating Sequencing Machines
PROFESSIONAL EXPERIENCE
Pfizer, Cambridge, MA
Co-op and Part-time Research Assistant, Rare Disease Research Unit
Research Objective: Understanding of the Molecular Pathways in Duchenne’s Muscular Dystrophy
! Conducted protein analysis of in vivo and in vitro samples
! Dissected and removed tissue samples from C57 Black 10 mice and assisted in the preparation and storage of samples for H&E
staining, protein quantification and fluorescent microscopy
! Optimized an array of antibodies used in Western Blot analysis
! Performed Western Blot analysis of proteins from mdx mouse skeletal muscle tissue samples
! Maintained cultures of C2C12 cells for viability and protein analysis
! Assisted in the development of a novel in vitro cell testing method for extracellular binding assays
! Harvested sets of treated cell culture samples for RNA quantification, cDNA preparation, and Real Time PCR to examine the
levels of target gene up regulation in treated groups compared to controls
Genzyme, A Sanofi Company, Framingham, MA
Undergraduate Research Co-op
Research Objective: Therapeutic Antibody Development
! Humanized mouse antibodies using PCR
! Synthesized working DNA samples from mini- and medi-preps of cultuered bacteria cells
! Confirmed DNA sequence changes in antibody constructs
! Performed Western Blot analysis of DNA samples for quality control testing of newly generated antibody sequences
! Transfected CHO cells with DNA constructs and detected antibody expression via Western Blot
Woods Hole Oceanographic Institution, Woods Hole, MA
Co-op Student and Research Assistant
Research Objective: Seasonal Observation of Red Tide Toxicity
! Assisted in the microscopic enumeration of archived phytoplankton samples for red tide organism Alexandrium fundyense
! Labeled and counted whole cell fluorescent in situ hybridization (FISH) assay of A. fundyense samples for analysis of real time
population levels in the Nauset system estuary
! Collected saltwater samples from the field at multiple depths and prepared the samples for short-term and long-term storage
INTERESTS & ACTIVITIES
Northeastern University Club Lacrosse, Guitar Player, Boston Sports Fan, Multi-Sport Athlete, Outdoor Exploration and Film
References Available Upon Request