Mrs Hyacinth Bernadette Lobo has over 30 years of experience in laboratory science and science education. She holds an MSc in Medical Molecular Biology and a BSc in Biochemistry/Microbiology. Her work experience includes positions as a science technician at various schools, a microbiology technician for Procter & Gamble, and research roles at University College London investigating dental diseases and rheumatoid arthritis. She has experience in techniques such as cell culture, DNA extraction, gel electrophoresis, and bacterial transformation. Currently she is interested in pursuing opportunities relating to health, nutrition, and molecular biology education.

1. CURRICULUM VITAE
Name: Mrs Hyacinth Bernadette Lobo
Address 7 Beechwood Drive Tel. No: 01689 823 194
Keston Mobile: 07954 177 170
Kent BR2 6HN Email: flobo0312@hotmail.com
Degrees: MSc. Medical Molecular Biology (Ratna Das Prize)
BSc. (Hons) Science – Biochemistry / Microbiology
Date of Birth: 1st July 1961 Nationality: British
WORK EXPERIENCE
Sept 2011 – July 2014 Science Technician
St Saviours & St Olaves, NewKent Road, London SE1 4AN
Covered all aspects of the delivery of science practicals from KS3 up to KS5,
specialising in Biology.
Having completed a training course on techniques and safety aspects in
Further School Microbiology run by CLEAPSS, the cloning of cauliflower
using aseptic techniques was successfully implemented as a class practical.
This was the first time this had been achieved and a couple of the plants
continued growing naturally once moved from the test tube to soil.
Also implemented basic molecular biology practicals for A level students
using Bio-Rad kits for: Forensic DNA Fingerprinting, pGLO Bacterial
Transformation and Green Fluorescent Protein (GFP) Chromatography.
Techniques introduced to the students included Horizontal Gel
Electrophoresis, SDS Polyacrylamide Gel Electrophoresis (PAGE) and
bacterial transformation with pGLO plasmid DNA which they found
interesting and inspiring.
Sept 2010 – July 2011 Science Technician
Kingsdale Foundation School, Alleyn Park Road Dulwich SE21 8SQ
Worked on all aspects of the delivery of science practicals from KS3 to KS5
as well as helping to demonstrate practicals such as the screaming jelly baby,
DNA extraction from onions, the Thermite reaction. Helped as cover
supervisor when science teaching staff were absent. Undertook all activities
pertaining to a school science prep room such as preparing practicals,
ordering and maintaining materials and equipment and preparing stock
solutions as well as making appropriate glassware.
May 2010 – Jul 2010 Microbiology Technician
Proctor & Gamble, Headley Avenue, Grays, Essex RM20 4AL
(Temp Via Manpower Services)
Worked in the P&G industrial complex at Thurrock in the Microbiology
sterile/clean lab, collecting fairy liquid samples and analysing for microbes

2. for quality analysis and quality control (QA/QC). Based on the analysis,
batch shipments were approved or rejected before export. Samples were also
analysed from throughout the process from raw materials, through to
production lines and packaging facilities for the presence of microbiological
contaminants. The techniques used included the RapiScreen Celsis method
and conventional plating out methods. Microbe species were identified using
gram positive and gram negative stains and standard identification kits. The
results were input into computer databases.
Oct 2009 – Mar 2010 Science Technician
Priory School, Tintagel Road, Orpington
Worked in a team of 5 technicians responsible for all laboratory activities
including HSE, using CLEAPSS procedures to prepare, set up and clear
materials, solutions, apparatus and specimens required for
experiments/demonstrations as requested by teaching staff. Ensured that
adequate supplies of reagents were available and designed and improved
various practicals such as the teabag diffusion practical and fixed apparatus
such as stopclocks, electrical leads and the laminator. Also maintained
bacterial cultures and agar for basic microbiology practicals.
Oct 2003 – Jan 2005 Science Laboratory Technician
Cator Park School for Girls, Lennard Road, Beckenham BR3 1QR
Responsible for all laboratory activities including: HSE using CLEAPSS
procedures, preparing, setting up and clearing of materials, solutions,
apparatus, specimens required for experiments / demonstrations as requested
by teaching staff and setting up and removal of teaching aids and ensuring
that adequate supplies of materials were available. Preparation of lesson
sheets and maintaining files relating to the same. Maintenance of good
records of all relevant activities.
Oct 1994 – Apr 1995 Research Technician
UCL Eastman Dental Institute 256 Gray’s Inn Road,
London WC1 8LD
Used histopathological staining of paraffin embedded sections of dental
biopsies after sectioning the tissues using a microtone. Also radioactive
probes were used in DNA and protein electrophoresis. Helped with various
analytical techniques to investigate and identify markers associated with
dental diseases/conditions.
Jan 1990 - Jan 1992 Research Assistant, Dept. ofClinical Immunology
UCLMS, Arthur Stanley House,Goodge St, London
(also London Hospital, Whitechapel, London )
I was sent on a one month long training program to Erasmus Hospital in
Bruxelles, Belgium under the supervision of Dr Marian Ludgate to train and
implement new microbial techniques in the Molecular Genetics Department

3. with Dr Ezio Bonifacio’s group at the London Hospital. On my return I
purchased and setup the equipment and reagents required for this research.
The title of my project was "Molecular Cloning in the identification of Islet
Cell Autoantigens in Type 1 diabetes. The work involved screening λgt11
thyroid and islet cDNA libraries as plaques on a lawn of transfected E.coli
Y1090 with diabetic sera and radioactive 32P nucleotide probes.
In conjunction with this study, serum of patients with high Islet Cell
Autoantibodies (ICA) were used to screen plaques using nitrocellulose filters
impregnated with IPTG and then detected using NBT/BCIP colour method.
Once the autoantigens were isolated, DNA sequencing would be carried out
to identify the sequences involved.
Oct 1987 - Dec 1989 Laboratory Scientific Officer
Rheumatology Unit, Dept. ofMedicine, Guys Hospital, London
Worked on the identification of HLA Class II antigens in Rheumatoid
Arthritis (became expert with tissue culture and protein chemistry
techniques). Commonly used procedures were 1 and 2D isoelectric focusing
(IEF), Polyacrylamide Gel Electrophoresis (PAGE), DNA hybridisation and
PCR. DNA was extracted from blood samples of Rheumatoid Arthritis
patients and restriction enzymes were used to analyse different markers
prevalent in the sera of patients from various ethnic backgrounds.
June 1983 - Oct 1983 Laboratory Assistant, Met. Police Forensic Science HQ,
Lambeth, London
Analysed, identified and catalogued glass samples from crime scenes using
oil and double diffraction techniques with a light microscope. Carried out
titrations and helped with fingerprint analysis as requested by senior staff.
Sept 1981 - June 1982 Laboratory Assistant, Hillcrest Chemicals, Sole Street, Kent
Tested resins and lubricants in a micro manufacturing facility.
EDUCATION
1972 - 1975 Gesc. Ermano Marchetti, Sao Paulo, Brazil
1976 - 1979 Upbury Manor Secondary School, Gillingham, Kent
1979 - 1980 Rainham Mark Grammar School, Rainham, Kent
1980 - 1981 Medway College of Design (Foundation Diploma in Art and Design)
1983 – 1985 Middlesex Polytechnic (DipHE)
1985 - 1987 University of Westminster (Bsc.)
2014 - 2015 University of Westminster (Msc.)
QUALIFICATIONS
1979 GCE 'O' Level - Maths, English, Portuguese, Art, Physics
1980 GCE 'O' Level - Chemistry, Human Biology, Biology
GCE 'A' Level - Art
1981 Foundation Diploma in Art and Design

4. 1985 Diploma of Higher Education (Science)
1987 BSc. (Hons) Biochemistry/Microbiology.
Modules taken: Microbiology, Biochemistry, Analytical Chemistry,
Antibiotics, Biotechnology, Immunology and Applied Enzymology. My 3rd
Year Project was: “The Development of a Plate Assay for detection of fungal
B-lactamases”.
2015 MSc. Medical Molecular Biology - Merit. Awarded the Ratna Das Prize.
This course covered cutting-edge methods used to study the molecular
mechanisms of diseases and their diagnosis and treatment using genetics, cell
signalling and molecular medicine. Bioinformatics was covered from
genome sequencing through to rational protein engineering for the
development of new therapeutics and personalized medicine. Other modules
covered were: Molecular and Cellular Therapeutics, Molecular Sciences and
Diagnostics, Principles of Molecular Medicine, Cell Signalling and Genetics.
Undertook an Extended Postgraduate Project (5 months) following my
interest in health and nutrition. The project investigated Adipose tissue
“browning” by Short Chain Fatty Acids and the effect of genes-environment
interactions in adipose tissue metabolism and their influence on optimal
health and chronic diseases. Techniques covered included cell culture,
protein extraction and Western blotting, RNA extraction, cDNA synthesis,
Real Time PCR, light microscopy, confocal microscopy and the Seahorse
Analyser.
SKILLS
Full Current Driving Licence.
Fluent in written and spoken Portuguese.
Illustrated three scientific booklets for Mr B. Shutes, published by Middlesex Polytechnic in 1983 and 1984:
 Trent Park Natural History Guide.
 The Vegetation of Icehouse Wood, Trent Park.
 The Invertebrates of Dew Pond and Icehouse Wood.
INTERESTS
Mother of 3 children (aged 24, 21 and 18), Hobbies include reading books on Health/Nutrition,
Painting/Drawing, Cooking, Gardening and listening to music.

5. REFEREES
Ms Maja Sabic Ms Evelyn Eagyepong
Head of Science Head of Biology (2nd
in charge Science)
Wilmington Grammar School for Girls Christ’s College
Parsons Lane East End Road
Wilmington Finchley
Kent DA2 7BB London N2 0SE
Email: smsabic@wgsg.co.uk Email: ema@ccfplus.com
majas76@yahoo.com Tel: 0208 3493581 ext.266
Tel: 01322 226351 Mob: 07712047391
Fax: 01322 22607
Mob: 07748860347
Personal Referees
Ms Lily Monteiro Mrs Tejal Inzani
Art Teacher Teaching Assistant
Kingsdale Foundation School 6 Norman Close
Alleyn Park Road Orpington
Dulwich Kent BR6 8EH
Email: mmr@kingsdale.co.southwark.sch.uk Email: Tejalinzani@hotmail.com
guche@btinternet.com Tel : 01689 861342
Mob: 07534 852721