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4. Admission Open-Offer30% # Job Placement consultancy # Projects & Training – All Biotech/Life Science/MBA/MCA/Engg/Medical/ Pharma Etc..UG/PG/Ph.D Contact:9952968059 [email_address]

9. CRC- TRAINING COURSES MODULE 1: Basic and Clinical Microbiology Techniques 1. Designing of Microbiology laboratory & Handling of instruments 2. Media preparation 3. Isolation of Microbes from various sources 4. Characterization of Microbes using conventional and modern techniques 5. Maintenance and Transport of culture 6. Phage titration assay 7. Growth curve 8. Bacterial conjugation DURATION – 1 week, Rs 4500 MODULE 2: Mycotechnology 1. Setting up of Mycology lab 2. Preparation of Various culture media 3. Isolation of Fungus from various environmental & clinical sample 4. Identification of fungus using conventional and molecular techniques

10. 5. Maintenance of fungal cultures 6. Extraction of DNA from Fungus and characterization using AGE 7. Extraction and characterization of Fungal proteins using SDSPAGE DURATION – 2 weeks Rs 6500 MODULE 3: Molecular Biology and Molecular Genetics Basic Module 1. Isolation of DNA from Bacteria, Fungi, Yeast & Plant 2. Isolation of RNA from Bacteria & Yeast 3. Agarose gel electrophoresis 4. DNA quantification using spectrophotometer 5. Plasmid DNA isolation from Bacteria 6. DNA elution technique 7. PCR 8. SDS-PAGE DURATION – 10 DAYS, Rs 3500

11. Advanced Module I 1. DNA fingerprinting i. RFLP ii. AFLP iii. RAPD iv. ERIC PCR 2. Different types of PCR i. RT PCR ii. Nested PCR 3. Protein profiling DURATION – 1 week Rs 6000 Advanced Module II 1.PCR detection of SNP & PNP 2.Detection of DNA contamination using PCR 3.Blotting techniques i. Southern Blotting ii. Northern Blotting iii. Western Blotting 4.Transformation DURATION – 1 week Rs 5000

12. Sophisticated Techniques* 1.Detection by autoradiography using radiolabelled probes 2.Transformation of DNA from prokaryotes to eukaryotes * - Enquire for details MODULE 4: Immunotechnology 1. Immunization and Schedule (theory) 2. Antigen and Antibody characterization Single Radial Immuno Diffusion ODD pattern Immunoelectrophoresis Rocket Immunoelectrophoresis Counter current Immunoelectrophoresis 3. Lymphocyte Preparation 4. Dot- ELISA

13. DURATION –1 week Rs 4000 MODULE 5: Plant Tissue Culture Techniques 1. Tissue Culture media preparation 2. Inoculation of Stem, Leaf, Root and Stem 3. Anther Culture 4. Embryo Culture 5. Agrobacterium Mediated Transformation 6. Protoplast Isolation 7. Identification of herbal plants for Anti-cancer, Anti-diabetic and Antimicrobial study 8. Phytochemical analysis of bioactive compounds 9. Detection of bioactive compounds using TLC,HPTLC & HPLC

14. DURATION – 1 week Rs 8000 MODULE 6: Animal Tissue Culture Techniques 1. Primary cell culture 2. Cryopreservation & Revival of cell lines 3. Maintenance of Cell lines 4. Subculture and Passaging of Cell lines 5. Identification of Virus – HA or HAI 6. Fixing of Cells for Staining 7. Determination of Cytotoxicity of Cell lines 8. Screening for Antiviral and Anticancer activity of Plant Extract 9. Estimation of Cytotoxicity by Biochemical Analysis (MTT assay)

15. DURATION – 1 week Rs 9000 MODULE 7: Chromatographic Techniques 1. Thin Layer (TLC) and Paper Chromatography 2. Column Chromatography 3. Gel Filtration chromatography 4. Ion exchange Chromatography 5. High Performance Thin Layer Chromatography (HPTLC) 6. High Performance Liquid Chromatography (HPLC) DURATION – 7 DAYS, Rs 8000

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19. CRC – Group RESEARCH CENTRE Microbiological analysis of samples with reference to bacteria, fungi, yeast, moulds & Actinomycetes Virulence anlaysis of pathogens PCR detection of pathogens Gene specific PCR Genetic diversity of the microbes RAPD PCR ERIC PCR Rep PCR BOX PCR 16 srRNA PCR analysis SDS PAGE analysis of proteins Western Blot analysis Enzyme profiling & all enzyme related studies

20. Extraction of compounds from plants & microbes Biological activity of compounds Antibacterial activity Antifungal activity Purification of biomolecules, proteins & enzymes Gel filtration chromatography Ion Exchange Chromatography Affinity Chromatography TLC & Bioautography Plant tissue culture based works Standardization & optimization of tissue culture plants for specific compounds Optimization of tissue culture raised plants Animal cell culture Cytotoxic analysis of compounds Antitumour evalution of compounds Evalution of Ahderence & invasiness of pathogens