Marimuthu Chinnarasu is seeking a career in biological research. He has a M.Sc. in Biotechnology and 5 years of work experience as a research fellow in molecular diagnostics and transplant immunology. His areas of research interest include molecular biology, cell biology, stem cell biology, and oncology. He has technical skills in various laboratory techniques including PCR, flow cytometry, gene expression analysis, and stem cell culture.

1. MARIMUTHU CHINNARASU PROFILE
Present Address: 6/32, Somasundaram Avenue, 3 cross street,Sakthi nagar, Porur,chennai-600116
Tamil nadu,India.
Permanent Address: Cauvery nagar(via), Kulathur(TK),Pudukkottai(Dt), Sathiyamangalam-622501,
Tamil nadu,IndiaT.amil na
CONTACT : +91 9003431833 MAIL : mariroshan@gmail.com
OBJECTIVE :
To explore my research knowledge and technical skills to build my career in a progressive institute that offers opportunitie s
to utilize my analytical skills and creativity to perform research work in the field of biological sciences.
Educational Qualifications:
PG : 2009-2011
M.Sc., Biotechnology, Department of Biotechnology, Bishop Heber College, Bharathidasan University, Trichy – 620 017,
Tamil nadu, India. CGPA 8.76 (88%) .
PG Diploma : 2009-2010
PG Diploma in Bioinformatics, Department of Biotechnology, Bharathidasan University, Bishop Heber College,
Trichy – 620 017,Tamil nadu, India, CGPA 8.57 (86%) .
UG : 2006-2009
B.Sc., Biotechnology, Department of Biotechnology, Bharathidasan University, Trichy - 620001,Tamil nadu, India ,
CGPA 7.56 (76 %) .
Work Experience
Total experience : 5 Years 6 Months
Current organization : Global Hospitals and Health city - Chennai,India
Designation : Senior Technical Officer and research fellow in Dept of Molecular Diagnostics & Transplant
immunology.
Duration : June 2013 topresent
Previous Designation : Research Assistant
Previous organization : Bharathidasan University
Research Areas & Interests :
Molecular Biology , Cell Biology , stem cell biology and Oncology.
ONCOLOGY: Oncogenetics , mutational studies and epigenetics analysis of cancer.
STEM CELLS: Induced Pluripotent Stem Cells(IPSCs) development for cancer treatment and organ transplantation.
Neutralization of harmful leukemic cells into harmless cells by transcription factors and Monoclonal Antibodies

2. Technical Skills :
Flow Cytometry , PCR, Q- PCR , RT-PCR
Sequencer, NGS , Gene xpert ,
Electrophoresis, SDS-page
DNA and RNA Extraction
Viral Nucleic Acid extraction
Nucleic Acid Purification
Infectious viral diseases screening and quantification
Cytogenetics , FISH
NGS library preparation
C-DNA Library Preparation
Elisa and Elispot, Immuno fluorescence
Western Blotting
Leukemia and lymphoma phenotype
All type of stem cells and other cells phenotype
Lymphocyte cross match
CDC cross match ,Cell cycle Analysis
Cell Viability Analysis , Stem Cell culture
Cyto toxicity analysis
Gene expression analysis
DNA Methylation Analysis
HLA typing
Clone development methods
Gene expression analysis
Protein separation & Purification
MolecularBiology andgenetics
DNA isolation, RNA isolation, plasmid and genomic DNA isolation, gene cloning from bacteria, site-directed mutagenesis,
Cloning, PCR, qPCR, DNA sequence analysis, Southern blotting, Northern blotting ,Vector design , primers design, digestions,
ligations, cloning. Cloning: transformation, reverse transcription, DNA purification, DNA gel analysis, quantification and
extraction ,Cytogenetics and FISH.
Immunological techniques :
Flow cytometery, ELISPOT, cytotoxic T cell assay, ELISA, lymphocyte proliferation assay, Estimation of various cytokines,
Neutrophil activation assay, Macrophage activation assay, Western blotting , Dot blot and Immunoblotting, raising
antibodies, purification of antibodies.
Cell Culture and Animal Models
Cell line maintenance, transfection optimization, plasmid transfection, RNAi knockdowns, Mouse and rat handling, oral
gavage, intraperitoneal and tail injections, small animal surgery
Current Clinical research Areas:
Epigenetics Analysis of disc degenerative disorders
Molecular analysis of infectious diseases
Flow cytometry cell lineage Analysis and phenotyping of different lineages
Role of hepatocyte stem cells in liver regeneration
In-vivo and In-vitro monitoring of Drug activities and it’s gene expression analysis

3. Assay Development and validation Skills:
Expert in Assay preparation ,standardization and validation : Enzyme Immunoassays, Cell and Molecular Biology assays
including PCR , Quantitative real time PCR and NGS, Flowcytometry , serological and other therapeutic assays.
 Identify the purpose ,Select the method used, Identify the method steps, Determine product specification limits,
Perform a risk assessment , Characterize the method , Complete method validation and transfer, Define the control
strategy, Train all analysts, Impact of the analytical method
PG Dissertation:
Antitumor effect of cisplatin on EAC(Ehrlich Ascites Carcinoma) Induced tumors in mice and it’s gene expression
analysis under the guidance of Dr.K. Premkumar , Department of Biomedical science Bharathidasan University-India.
PUBLICATIONS:
Thermostable alkaline protease from thermophilic and alkaliphilic Bacillus licheniformis and its application in International
Journal of Medicine and Biosciences -Int J Med Biosci. 2012; 1(3): 18 – 26 ,www.ijmbonline.com.
Waiting publications:
Molecular Profiling of genes involved in liver and cardiac engraftment rejection .
Eubacterial and panfungal genome analysis and it’s applications in clinical diagnosis.
A parametric study on the bixin oil suspensions produced using Annatto seeds (Bixia orella) and its potential application .
REFERENCES:
Name
First referee Second referee Third referee
Dr.Mrs.Violet Dhayabaran Dr.R.Jasmine Dr. Beatrice Valdaris
Position/
institution
Head & Coordinator,
Dept of Biotechnology,
Bishop Heber College,
Trichy.
Associate professor,
Dept of Biotechnology,
Bishop Heber College,
Trichy.
Associate professor,
Dept of Biotechnology,
Bishop Heber College,
Trichy.
Phone (+91)9994291637, (+91)9500423739 (+91)9655775337
E-mail violetstaff@yahoo.co.in jasmine_selvakumar@yahoo.com beatricevaldaris@gmail.com
DECLARATION:
I hereby declare that the above-mentioned information is correct up to my knowledge and I bear the
responsibility for the correctness of the above- mentioned particulars.
Place : Chennai
Date :
Yours truly
MARIMUTHU CHINNARASU