The document discusses safety procedures for working in a class II laminar flow cabinet. It describes the components and functioning of 2-filter vs 3-filter cabinets, including air flow and filtration. Guidelines are provided for working with pathogens, such as cleaning surfaces before and after use and not using the cabinet for storage. Detailed cleaning procedures are outlined for cabinet use including disinfecting in the morning before starting work and thoroughly cleaning in the evening before decontamination. Potentially contaminated areas of the cabinet are identified.

1. A Difference in Life Science
Safety work
in a
laminarflow
cabinet class II

2. A Difference in Life Science
- Function of a laminar flow 2 filters vs 3 filters
- How to work with pathogens
- Use and clean a cabinet from the start to the end
Safety work in a laminarflow cabinet class II

3. A Difference in Life Science
- Function of a laminar flow 2 filters vs 3 filters
- How to work with pathogens
- Use and clean a cabinet from the start to the end
Safety work in a laminarflow cabinet class II

4. Laboratory devices
Cabinet selection / types
• Clean Bench
- Open fronted exhaust protective hood
- Work protection only
• Class I
- Open fronted exhaust protective hood
- Operator / environmental protection
• Class II
- Vertical laminar flow recirculating air cabinet
- Operator / work / environmental protection
• Class III
- Enclosed glove box with filtered air in and out
- Operator / work / environmental protection
A Difference in Life Science

5. A Difference in Life Science
Laboratory devices
Cabinet selection / types
• Clean Bench
- Open fronted exhaust protective hood
- Work protection only
• Class I
- Open fronted exhaust protective hood
- Operator / environmental protection
• Class II
- Vertical laminar flow recirculating air cabinet
- Operator / work / environmental protection
• Class III
- Enclosed glove box with filtered air in and out
- Operator / work / environmental protection

6. HEPA filter
A Difference in Life Science

7. HEPA stands for High Efficiency Particulate Air filter.
They are normally 99.999% efficient against a particle
size of 0.3µm. (Eurovent EN 13091:1999, EN1822-1
Class H-14 or higher.)
A Difference in Life Science
HEPA filter

8. HEPA Filter construction
Air flow
Filter Medium
Gasket
Filter Frame
A Difference in Life Science

9. Pre-filter Blower Plenum HEPA Work Area
Filter
Filter Technology - Airflow Principle
A Difference in Life Science

10. Filter Technology - Airflow Principle
A Difference in Life Science
Blower
Plenum
HEPA Filter
Work Area

11. A Difference in Life Science
Blower
HEPA Filter
Work Area
2-Filter Cabinet
Plenum
Bio Safety Cabinet Class II
- Air flow 70% recirculation 30% exhaust

12. A Difference in Life Science
Blower
HEPA Filter
Work Area
2-Filter Cabinet
Plenum
Pre-Filter
Bio Safety Cabinet Class II
- Air flow 70% recirculation 30% exhaust

13. Bio Safety Cabinet Class II
- Vertical laminar flow recirculating air cabinet
- Air flow 70% recirculation 30% exhaust
- Operator / work / environmental protection
A Difference in Life Science
2-Filter Cabinet 3-Filter Cabinet
Pre-Filter

14. Bio Safety Cabinet Class II
- Air flow 70% recirculation 30% exhaust
A Difference in Life Science
2-Filter Cabinet 3-Filter Cabinet
Pre-Filter
1st-Filter
H14

15. Effects by down flow speed
0.25 - 0.50m/s According EN 12469
• Too low speed (< 0.25 m/s) - less clean air at work place
• Too high speed (> 0.50 m/s) - turbulences at work table
A Difference in Life Science

16. A Difference in Life Science
Positive pressure
Working principle
Negative - Positive pressure A2 Cabinet
Negative pressure
Blower
Filters
2-Filter Cabinet 3-Filter Cabinet

17. Common particle generation
Sitting or standing no movements
Sitting small movements
of head, hands and arms
Siitting small movements
of upper body, arms and feet
Standing up
and sitting down
Slow walk (ca. 0.9 m/s)
Normal walk (ca. 1.6 m/s)
Fast walk (ca. 2.3m/s)
Particle size per m3 / Min
(0.3 um) (0.5 um)
004 Mio 001 Mio
0
17 Mio 005 Mio
0
35 Mio 010 Mio
87Mio 026 Mio
175 Mio 052 Mio
262 Mio 073 Mio
350 Mio 105 Mio

19. A Difference in Life Science
- Function of a laminarflow 2 filters vs 3 filters
- How to work with pathogens
- Use and clean a cabinet from the start to the end
Safety work in a laminarflow cabinet class II

20. Biosafety Level
BSL 1 - Little or no risk
BSL 2 - Moderate risk Prophylaxis and treatment available
BSL 3 - High risk. Prophylaxis and treatment if treated early enough available
BSL 4 - Very high risk. No treatment available
Typical Examples:
Group 2 - Botulism, Legionella, E.coli strains
Group 3 - Anthrax, Typhoid, HIV, Hepatitis, BSE
Group 4 - Lassa fever, Ebola, Green Monkey Disease
A Difference in Life Science

21. BSL 1 - Little or no risk
BSL 2 - Moderate risk Prophylaxis and treatment available
BSL 3 - High risk. Prophylaxis and treatment if treated early enough available
BSL 4 - Very high risk. No treatment available
Typical Examples:
Group 2 - Botulism, Legionella, E.coli strains
Group 3 - Anthrax, Typhoid, HIV, Hepatitis, BSE
Group 4 - Lassa fever, Ebola, Green Monkey Disease
A Difference in Life Science
Biosafety Level

22. Working safely with pathogens
• Before bring in and take out - clean with ETOH 70%
• Use 2 gloves together (specially with nanoparticle)
• Do not operate the cabinet if any of the alarms are activated
• A bunsen burner should not be used in a cabinet at all
• Do not use the cabinet as a storage area
• Minimize disturbances to airflow barrier
• Observe correct sash opening height (20 cm)
• Observe surface – decontamination (periodically clean with ETOH 70%)
• The Cabinet must be controlled annually
(down flow speed, leektesting of the hepa filter etc)
A Difference in Life Science

23. Working safely with pathogens
• Before bring in and take out - clean with ETOH 70%
• Use 2 gloves together (specially with nanoparticle)
• Do not operate the cabinet if any of the alarms are activated
• A bunsen burner should not be used in a cabinet at all
• Do not use the cabinet as a storage area
A Difference in Life Science

24. Working safely with pathogens
• Before bring in and take out - clean with ETOH 70%
• Use 2 gloves together (specially with nanoparticle)
• Do not operate the cabinet if any of the alarms are activated
• A bunsen burner should not be used in a cabinet at all
• Do not use the cabinet as a storage area
Do not use the cabinet as a storage area
A Difference in Life Science

25. Working safely with pathogens
• Before bring in and take out - clean with ETOH 70%
• Use 2 gloves together (specially with nanoparticle)
• Do not operate the cabinet if any of the alarms are activated
• A bunsen burner should not be used in a cabinet at all
• Do not use the cabinet as a storage area
• Minimize disturbances to airflow barrier
• Observe correct sash opening height (20 cm)
• Observe surface – decontamination (periodically clean with ETOH 70%)
• The Cabinet must be controlled annually
(down flow speed, leektesting of the hepa filter etc)
A Difference in Life Science

26. A Difference in Life Science
- Function of a laminarflow 2 filter vs 3 filter
- How to work with nanoparticles and pathogens
- Use and clean a cabinet from the start to the end
Safety work in a laminarflow cabinet class II

27. Cabinet work procedures
Morning
• Clean work table surface with 70% alcohol
• Cabinet must run min. 15 min before working
During work day
• Minimum stand by mode
Evening
• Clean down work surface & spill tray with 70% alcohol
• Cabinet must run 15 min after cleaning
• Decontaminate with UV-light for min. 15 min
• Cabinet can run on stand by mode during night
A Difference in Life Science

28. Cabinet work procedures
Morning
• Clean work table surface with 70% alcohol
• Cabinet must run min. 15 min before working
During work day
• Minimum stand by mode
Evening
• Clean down work surface & spill tray with 70% alcohol
• Cabinet must run 15 min after cleaning
• Decontaminate with UV-light for min. 15 min
• Cabinet can run on stand by mode during night
A Difference in Life Science

29. A Difference in Life Science
2-Filter Cabinet 3-Filter Cabinet
possible contaminated areas
Cabinet work procedures:
Morning procedure – before first start

30. A Difference in Life Science
2-Filter Cabinet 3-Filter Cabinet
possible contaminated areas
Cabinet work procedures:
Morning procedure – ready for work

31. Cabinet work procedures
Morning
• Clean work table surface with 70% alcohol
• Cabinet must run min. 15 min before working
During work day
• Minimum stand by mode
Evening
• Clean down work surface & spill tray with 70% alcohol
• Cabinet must run 15 min after cleaning
• Decontaminate with UV-light for min. 15 min
• Cabinet can run on stand by mode during night
A Difference in Life Science

32. Cabinet work procedures
Morning
• Clean work table surface with 70% alcohol
• Cabinet must run min. 15 min before working
During work day
• Minimum stand by mode
Evening
• Clean down work surface & spill tray with 70% alcohol
• Cabinet must run 15 min after cleaning
• Decontaminate with UV-light for min. 15 min
• Cabinet can run on stand by mode during night
A Difference in Life Science

33. A Difference in Life Science
2-Filter Cabinet 3-Filter Cabinet
Still possible contaminated areas
Cabinet work procedures:
Evening procedure

34. A Difference in Life Science
2-Filter Cabinet 3-Filter Cabinet
Still possible contaminated areas
Cabinet work procedures:
Evening procedure – after cleaning below table top

35. A Difference in Life Science
2-Filter Cabinet 3-Filter Cabinet
Cabinet work procedures:
Evening procedure – after last run

36. Cabinet work procedures
Morning
• Clean work table surface with 70% alcohol
• Cabinet must run min. 15 min before working
During work day
• Minimum stand by mode
Evening
• Clean down work surface & spill tray with 70% alcohol
• Cabinet must run 15 min after cleaning
• Decontaminate with UV-light for min. 15 min
• Cabinet can run on stand by mode during night
A Difference in Life Science

37. A Difference in Life Science
Thanks for your attetion

38. A Difference in Life Science
For any further question,
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