RFLP

1. Restriction Fragment Length
Polymorphism (RFLP)
Unlocking the Secrets of DNA
BY: Suman

2. Introduction
• - RFLP = DNA Detective Tool!
• - Detects differences in DNA by cutting it with
restriction enzymes.
• - Used in forensic science, medical diagnosis,
and genetic research.

3. Principle
• - DNA cut at specific sequences → fragments
of varying lengths.
• - Variations create unique patterns.
• - Separated by gel electrophoresis for analysis.

4. Steps in RFLP
• 1. Extract DNA
• 2. Cut with Restriction Enzymes
• 3. Gel Electrophoresis
• 4. Southern Blotting
• 5. Hybridization with Probe
• 6. Visualization

5. Applications
• - Forensics: Criminal ID & Paternity Testing
• - Medicine: Detect genetic disorders
• - Evolution: Study genetic variations
• - Agriculture: Breed better crops/animals

6. Conclusion
• - RFLP = Powerful tool in genetics.
• - Helps us read the hidden codes of life.
• - Still relevant for teaching, research, and
diagnostics.