RFLP (Restriction Fragment Length Polymorphism) is a DNA profiling technique that involves using restriction enzymes to cut DNA into fragments of varying lengths, separating the fragments via gel electrophoresis, and comparing fragment patterns to detect differences between individuals' DNA. It was an early and important method for genome mapping, disease risk determination, and forensic identification but has been replaced by newer techniques as it is expensive, time-consuming, and requires large DNA amounts. RFLP analysis cuts DNA with restriction enzymes, separates fragments by size via gel electrophoresis, and compares patterns to detect length polymorphisms indicating sequence differences between individuals.