This document discusses cellular respiration in plants. It covers:
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2) There are three main stages of respiration - glycolysis, the TCA cycle, and the electron transport chain. Glycolysis occurs in the cytoplasm and partially breaks down glucose, the TCA cycle fully breaks down pyruvate in the mitochondria, and the electron transport chain generates ATP using an proton gradient across mitochondrial membranes.
3) The efficiency of aerobic respiration is around 40% while anaerobic respiration is only around 2% efficient due to the lower ATP yield. A variety of factors like
in details about the transport mechanism of Plants as per NCERT syllabus Class- XI. it includes why transport in plants is required and necessary,active n passive transport, diffusion, osmosis, facilitated diffusion, mass flow hypothesis, transpiration and its related theory,plant water relationship, water potential, symport,antiport ,uniport. proper diagrams and important definitions
in details about the transport mechanism of Plants as per NCERT syllabus Class- XI. it includes why transport in plants is required and necessary,active n passive transport, diffusion, osmosis, facilitated diffusion, mass flow hypothesis, transpiration and its related theory,plant water relationship, water potential, symport,antiport ,uniport. proper diagrams and important definitions
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This presentation explains DPD (diffusion pressure deficit), Plasmolyses and Imbibition in details. this also include the numericals related to Water potential. difference between DPD & water potential.
This presentation is about gaseous exchange. Especially about gases exchange in plants. in this presentation we discussed about that how gases exchange occur in different plant and through which organs gases exchange occur in plants.
Students able to understand that who helps to transport in plants, Mechanism of transport in plants, physical forces involved in transport, Behavior with different solutions.
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photosynthesis, the process by which green plants and certain other organisms transform light energy into chemical energy. During photosynthesis in green plants, light energy is captured and used to convert water, carbon dioxide, and minerals into oxygen and energy-rich organic compounds.
This ppt covers all the basic and important points of chapter respiration in higher plants .
It will help to revise all the important concepts and points of this chapter quickly .
As this is one of the most important and long chapter of plant physiology so se, concised it into short to make easy to ready.
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Respiration in plants
1. Respiration
• Biological process whereby the energy
stored in carbohydrates from PS is released
in a step-wise, controlled manner.
• Energy released is coupled to the synthesis
of ATP.
• ATP is essential for plant cell maintenance,
growth and development
3. Equation for Aerobic Respiration
C6H12O6 + 6O2 + 6H2O 6CO2 + 12H2O + energy
(glucose) (ATP)
1 mole glucose 36 ATP
4. Efficiency of Aerobic Respiration
• ADP-P bond releases -7.6 kcal/mol ATP
when bond is broken
• Theoretical energy yield from burning 1mol
glucose in a calorimeter = -686 kcal/mol
• Practical yield from burning 1mol of glucose
in the cell with oxygen = 36ATP
36 ATP X -7.6 kcal/mol = -274 kcal/mol glucose
274/686 kcal/mol X 100 = 40% efficiency
5. Efficiency of Anaerobic Respiration
• ADP-P bond releases -7.6 kcal/mol ATP
when bond is broken
• Theoretical energy yield from burning 1mol
glucose in a calorimeter = -686 kcal/mol
• Practical yield from burning 1mol of glucose
in the cell without oxygen = 2 ATP
2 ATP X -7.6 kcal/mol = -15.2 kcal/mol glucose
15.2/686 kcal/mol X 100 = 2.2% efficiency
6. 3 Stages of Respiration
• Glycolysis
• TCA Cycle
• Electron Transport Chain
7. Glycolysis
• Occurs in all living organisms
• Only stage which can occur without oxygen
• Oldest stage of respiration
operated for billions of years in anaerobic organisms
• Converts glucose to 2 pyruvates in cytosol
with O2 goes on to TCA cycle
without O2 pyruvate is converted to lactate or ethanol
(fermentation)
• Yields 2ATP/mole glucose in the absence of O2
15. 3 Stages of Respiration
• Glycolysis
cytoplasm
with or without oxygen present
breaks glucose (6C) into 2 pyruvates (3C)
• TCA Cycle
mitochondrial matrix
only if oxygen present
converts pyruvate via acetyl CoA into CO2; generates
NADH and FADH2
• Electron Transport Chain
mitochondrial membranes = cristae
transfers electrons from NADH and FADH2 to reduce
O to H O and generate ATP
16. Mitochondria
• Spherical to oval
about 1 micron diameter
# mito./cell increases with demand for
respiration; 300-1000/root tip cell
• Double-membrane bound
outer smooth
inner folds forming cristae
controls movement in/out
site of electron transportm
• Matrix
soluble phase
site of TCA cycle; DNA, RNA, ribosomes
matrix
cristae
17. Alternate Fates of Glucose C
• Not all C respired to CO2
• Intermediates of respiration branch off:
amino acids
pentoses for cell wall structure
nucleotides
porphyrin biosynthesis
fatty acid synthesis
lignin precursors
precursors for carotenoid synthesis, hormones
18. Factors Affecting Resp. Rate
• [Substrate]
• [ATP]
• [Oxygen]
• Temperature
• Plant type
• Plant organ
• Plant age
19. Factors: Substrate Availability
• Resp. higher right after sundown compared
to right before sunrise due to [S]
• Shaded leaves respire slower than lighted
leaves
• Starvation of plant tissue results in
utilization of proteins
• High [ATP] in cell and get negative
feedback on resp.
20. Factors: [Oxygen]
• No effect until [O2] < 1%
Cyt oxidase not sensitive to O2 until 0.05%
• O2 diffuses in water 10,000 X slower than in air
• Some plants have intercellular air system, e.g.,
aerenchyma in shoots and roots (rice)
• Very low levels of O2 see accelerated
breakdown of sugars to ethanol and CO2
evolved = Pasteur Effect
21. Factors: Temperature
• Q10 for respiration is 2.0 - 2.5 between 5 and
25C
• Q10 = rate of process at one temperature
divided by the rate at 10C lower temp.
Decreases with most plant tissues at 30-35C
O2 being used so fast, it can’t diffuse fast enough
into tissues
• Tropical regions - 70-80% PS C lost to resp.
due to high night temperatures and resp.
rates
22. Factors: Plant Type/Organ/Age
• Resp. rate tends to increase with age of plant
Young trees lose about 1/3 daily PS C to resp. and
doubles with older trees as ratio of PS/Non-PS
tissue decreases
• Greater metabolic activity = greater resp. rates
Root tips, dev. buds and meristematic regions in
general have higher respiration rates
In veg. tissues, resp. decreases from the tip to the
mature regions
• Seeds - low resp. rates, dormant, desiccation
results in slowdown of respiration
23. Factors: Plant Type/Organ/Age (cont.)
• Ripening Fruit
Resp. high when young cells are dividing and growing
• Climacteric Fruit (apples, tomatoes)
Sharp increase in rate immediately before fruit ripening
= climacteric rise in respiration
Coincides with full ripeness and flavor and preceded by
huge increase in ethylene production
This leads to senescence and decrease in respiration
• Non-climacteric Fruit
Citrus, cherries, grapes, pineapple, strawberries
Insensitive to ethylene
24. Controlled Atmosphere Storage
• Lower O2 (2% - 3%) & raise CO2(5% - 10%)
slows down resp.
• No ethylene
high CO2 also inhibits ethylene synthesis
• Temps. typically about -1 to -0.5C
• Pick apples in Sept./Oct. when green and
immature and store in CA
expose to normal air with ethylene when ready to
sell fresh apples in March
25. Cyanide Resistant Respiration
• Aerobic resp. (cyt oxidase) in plants and
animals inhibited by CN-
and N3
-
(azide)
bind to Fe in enzyme and halts e-
transport
• Animals: CN causes resp. to decrease fast,
virtually irreversible and fatal
• Plants: display a 10-25% CN-resistant resp.
and alternate pathway for electron flow
electron flow branches off to alternate oxidase
less ATP produced
26. Cyanide Resistant Respiration (cont.)
• Metabolic Role?
No clear role
Operates when cyt oxidase poisoned
Energy overflow hypothesis
overflow for electrons when resp. rate exceeds
demand for ATP; high with high carbo. levels
Skunk cabbage, Voodoo lily, Stinking lily: CN-
res. pathway causes temp. of spadix to increase
10-20C.
volatilization of odiferous cmpds which attract
pollinators
Biological process whereby reduced organic compounds are mobilized and oxidized, energy released is stored in ATP. Glucose considered the direct substrate, but other CHO’s are degraded to form glucose