This document describes a methodology for isolating cimetidine, a histamine H2 receptor antagonist used to treat stomach ulcers, from human plasma using HPLC for quantitative analysis. The objectives are to understand cimetidine's mode of action and effects of overdose, and to develop an HPLC method for analyzing spiked plasma samples. The methodology uses HPLC with a C18 column, UV-Vis detector at 230nm, and mobile phase flow rate of 1.0 mL/min to separate and quantify cimetidine. Sample preparation involves preparing standard solutions and extracting cimetidine from plasma using solid phase, liquid-liquid, or protein precipitation extraction for HPLC analysis.

1. Isolation of Cimetidine fromHuman Plasma for HPLC
Analysis

2. Contents
 Introduction
 Objectives
 Methodology
 Conclusion

3. Introduction
 Cimetidine is a histamine H2 receptor
antagonist.
 H2-receptors located on the gastric parietal
cells.
 Cures stomach ulcer.

5. Properties of Cimetidine

6. But How Can Over dosage of Cimetidine Effect
Human Plasma ???

7. Cont.…..
 Increase the serum concentration when
interact with other Drugs.
 Decreases the level of Iron (Fe).

8. Objectives
 Mode of action of cimetidine
 Preparation of standard solutions and
sample solution.
 HPLC method used for the analysis of
spiked plasma samples.
 Quantitative determination of cimetidine
by spectroscopic techniques( HPLC).

9. Methodology
 HPLC is highly selective and sensitivity
analytical technique.
 Prefer for the qualitative and quantitative
analysis of Cimetidine.
 It separates, identifies, and quantifies
components in a mixture.

10. Chromatographic condition
 HPLC system will be used for detection.
 C18 column
 UV-Vis detector at 230 nm wavelength.
 Programme-controlled auto injector 20μL
sample will be injected.
 Chromatographic temperature and flow
rate at 1.0 mL/min.

11. Standards
 0.1 mg/mL stock standard solutions of
cimetidine are prepared.
 Human plasma samples will be prepared
by spiking suitable amount of the working
standard solutions into drug free human
plasma.

12. Extraction Methods
 Solid Phase Extraction (SPE)
 Liquid Liquid Extraction (LLE)
 Protein Precipitation (PP)

13. Solid Phase Extraction
HPLC Analysis

14. Liquid Liquid Extraction

15. Protein Precipitation

16. Conclusion

17. References
 Jacqueline B, Rosenthal L (2 December 2014). Lehne's Pharmacology for
Nursing Care. Elsevier Health Sciences. pp. 952–. ISBN 978-0-323-34026-7.
 Fischer J, Ganellin CR (2006). Analogue-based Drug Discovery. John
Wiley & Sons. p. 444. ISBN 9783527607495.
 https://www.sciencedirect.com/science/article/pii/S2666166721005621
 Dart RC (2004). Medical Toxicology. Lippincott Williams & Wilkins. pp. 402–
. ISBN 978-0-7817-2845-4.
 Rosenfeld GC, Loose DS (2007). Pharmacology. Lippincott
Williams & Wilkins. pp. 202–. ISBN 978-0-7817-8074-2.