This document discusses experimental research methodology, specifically focusing on variance, sources of error, and control techniques. It defines variance as a measure of dispersion among scores and explains how research design aims to maximize systematic variance from the experimental manipulation while controlling extraneous and minimizing error variance. Extraneous variance comes from irrelevant variables and can be controlled through randomization, elimination, matching, or statistical control. Error variance results from uncontrollable individual differences among subjects and errors of measurement. The goal is for research design to provide valid answers to research questions in an accurate and cost-effective manner.
Experimental Research Design (True, Quasi and Pre Experimental Design)Alam Nuzhathalam
Experimental Research Design., Introduction, Definition, Characteristics and Classification (True Experimental Research Design, Quasi Experimental Research Design and Pre Experimental Research Design)..
Field Study : learning objectives -
Explains what is field work?
Explains different field study variants
Identity principal of field work
How field work differ from laboratory work
Explains different methods used in field work
Explains different sampling methods
Know how to prepare and run a field session
Identity good interview questions
Know what to do after captured data
01 parametric and non parametric statisticsVasant Kothari
Definition of Parametric and Non-parametric Statistics
Assumptions of Parametric and Non-parametric Statistics
Assumptions of Parametric Statistics
Assumptions of Non-parametric Statistics
Advantages of Non-parametric Statistics
Disadvantages of Non-parametric Statistical Tests
Parametric Statistical Tests for Different Samples
Parametric Statistical Measures for Calculating the Difference Between Means
Significance of Difference Between the Means of Two Independent Large and
Small Samples
Significance of the Difference Between the Means of Two Dependent Samples
Significance of the Difference Between the Means of Three or More Samples
Parametric Statistics Measures Related to Pearson’s ‘r’
Non-parametric Tests Used for Inference
Experimental Research Design (True, Quasi and Pre Experimental Design)Alam Nuzhathalam
Experimental Research Design., Introduction, Definition, Characteristics and Classification (True Experimental Research Design, Quasi Experimental Research Design and Pre Experimental Research Design)..
Field Study : learning objectives -
Explains what is field work?
Explains different field study variants
Identity principal of field work
How field work differ from laboratory work
Explains different methods used in field work
Explains different sampling methods
Know how to prepare and run a field session
Identity good interview questions
Know what to do after captured data
01 parametric and non parametric statisticsVasant Kothari
Definition of Parametric and Non-parametric Statistics
Assumptions of Parametric and Non-parametric Statistics
Assumptions of Parametric Statistics
Assumptions of Non-parametric Statistics
Advantages of Non-parametric Statistics
Disadvantages of Non-parametric Statistical Tests
Parametric Statistical Tests for Different Samples
Parametric Statistical Measures for Calculating the Difference Between Means
Significance of Difference Between the Means of Two Independent Large and
Small Samples
Significance of the Difference Between the Means of Two Dependent Samples
Significance of the Difference Between the Means of Three or More Samples
Parametric Statistics Measures Related to Pearson’s ‘r’
Non-parametric Tests Used for Inference
WHAT IS METHODOLOGY?
WHAT IS RESEARCH?
WHAT IS RESEARCH METHODOLOGY?
STUDY DESIGNS
WHAT IS DESCRIPTIVE STUDY?
WHAT IS ANALYTICAL STUDY?
CONCLUSION
REFERENCES
The need for good research is to find the best evidence for clinical
practice, for specific problems, and to address methods in reducing the
burden of illness on a larger scale.
It should reflect the aspirations and expectations of the research topic.
The slides will help you in knowing the components of research design in brief what is research design, components of research design, differnt types of research design
Understanding The Experimental Research Design(Part I)DrShalooSaini
This Power Point Presentation has been made while referring to the research books written by eminent, renowned and expert authors as mentioned in the references section. The purpose of this Presentation is to help the research students in developing an insight about the Experimental Research Design(Part –I).
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
2. INDEX PAGE
1. Introduction 3
2. Research Design 4
Meaning of Research Design 4
Function of a Research Design 5
3. Concept of variance 5
4. Partitioning of variance 6
Systematic variance
Extraneous variance 6
Randomization 7
Elimination 7
Matching 8
Statistical Control 8
Error variance 9
5. Source of error variance 9
6. Control Techniques 10
7. Conclusion 11
8. Reference 11
3. INTRODUCTION
Research in common parlance referred to as search for knowledge. It can also be defined as a
scientific and systematic search for pertinent information on a specific topic. In fact, research is
an art of scientific information. Redman and Mory defines research as a “systematized effort to
gain new knowledge.”Some people consider research as a movement from the known to the
unknown. According to Clifford Woody research comprises defining and redefining problems,
formulating hypothesis or suggesting solution; collecting, organizing and evaluating data;
making deductions and reaching conclusions; and at last carefully testing the conclusion to
determine whether they fit the formulating hypothesis. Research is, thus, an original
contribution to the existing stock of knowledge making for its advancement. It is the persuit of
truth with the help of study, observation, comparison and experiment. In short, the search for
knowledge through objective and systematic method of finding solution to a problem is
research. As such the term ‘research’ refers to the systematic method consisting of enunciating
the problem, formulating a hypothesis, collecting the facts or data, analyzing the facts and
reaching certain conclusions either in the form of solutions(s) towards the concerned problem
or in certain generalizations for some theoretical formulation.
The purpose of research is to discover answers to questions through the
application of scientific procedures. The main aim of research is to find out the truth which is
hidden and which has not been discovered as yet.
4. Every science has goals. In physics, the goals are concerned with learning how the
physical world works. In astronomy, the goals are to chart the universe and understand both
how it came to be and what it is becoming.
The goals of psychologist conducting basic research are to describe, explain, predict
and control behavior.
Description: What is Happening?
Explanation: Why is it Happening?
Prediction: When Will it Happen Again?
Control: How can it be Changed?
RESEARCH DESIGN
MEANING OF RESEARCH DESIGN
Research design is the plan, structure and strategy of investigation conceived so as to obtain
answers to research questions and to control variance. The plan is the overall scheme or the
program of the research. It includes an outline of what the investigator will do from writing the
hypothesis and their operational implications to the final analysis of data. Structure of the
research is outline of the research design, and the scheme is the paradigm of operation of the
variable. Strategy includes the methods to be used to gather and analyze the data. In other
words, strategy implies how the research objective will be reached and how the problems
encountered in the research will be tackled. (Kerlinger, 2007)
5. A traditional research design is a blueprint or detailed plan as to how a research
study is to be completed. That is, how it would operationalise variables so that they can be
measured, how to select a sample of interest to the research topic, how to collect data to be
used as a basis for testing hypothesis, and how to analyze the results. (Thyer, 1993)
FUNCTIONOF A RESEARCH DESIGN
The function of a research design is:
To provide answer to research question and
To enable the researcher to answer question as validly, accurately and as economically
as possible.
CONCEPTOF VARIANCE
Variance is a measure of the dispersion or spread of a set of scores. It describes the extent to
which the scores differ from each other. Variance and variation, though used by synonymously,
are not identical terms. Variation is a more general term which includes variance as one of the
statistical methods of representing methods. The main technical function of research design is
to control variance. Research design is a set of instructions to the investigator together analyze
data in certain ways. Therefore, research design acts as control mechanism and enables the
researcher to control unwanted variances. Variance control is a central theme of research
design.
6. PARTITIONING OF VARIANCE
The researcher is directly concerned with three types of variance namely experimental
variance, extraneous variance and error variance. Main functions of research design are to
maximize the effect of systematic variance, control extraneous variance and minimize error
variance. A discussion of these variances is presented below.
SYSTEMATIC VARIANCE: By constructing an efficient research design the investigator attempts
to maximize the variance of the variable of substantive research hypotheses. Systematic
variance is the variability in the dependent measure due to the manipulation of the
experimental variable by the experimenter. An important task of the experimenter is to
maximize this variance. This objective is achieved by making the level of the experimental
variable as unlike as possible. Suppose an experimenter is interested in studying the effect of
intensity of light on visual acuity. The experimenter decides to study the effect by manipulating
three levels of light intensity, i.e. 10ml, 15ml, 20ml. as the differences between any two levels
of the experimental variable is not substantial, and there is little chance of separating its effect
from the total variance. Thus, in order to maximize systematic variances, it is desirable to make
the experimental conditions (levels) as different as possible. In this experiment it would be
appropriate, then to modify the levels of light intensity to 10ml, 20ml, 30ml so that the
difference between any two levels is substantial.
EXTRANEOUS VARIANCE
Extraneous variance is produced by the extraneous variables or the relevant variables. An
experimenter always tries to control the relevant variables and thus, also wants to eliminate
7. the variances produced by these variables. For elimination of extraneous variance it is essential
that the extraneous variables be properly controlled. There are four ways to control the
extraneous variances.
1. Randomization: It is considered to be the most effective way to control the variability
due to all possible extraneous sources. It is a procedure for equating groups with
respect to secondary variable. Randomization means random selection of the
experimental units from the larger population. Random assignment means that every
experimental unit has an equal chance of being placed in any of the treatment
conditions or group. In using randomization method some problems may be
encountered. It is possible to select a random sample from a population, but then
assignment of experimental units to groups may get biased. Random assignment of
subjects is critical to internal validity. If subjects are not assigned randomly, confounding
may occur.
Randomized group design and randomized block design are the examples of research
design in which randomization is used to control the extraneous variable.
2. Elimination: this procedure is the easiest way to controlling the unwanted extraneous
variable through elimination of variable. Suppose, the sex of the subject as unwanted
secondary variable, is found to influence the variable in an experiment. Therefore the
variable of sex has to be controlled. The researcher may decide to take either all males
8. and all females in an experiment and thus, controlled through elimination the variability
due to the sex variable.
By using elimination for controlling the extraneous variables, researcher looses
the power of generalization. If the researcher selects the subject from a restricted range then
the researcher can generalize the results within restricted range and not outside it. Elimination
procedure is used in non-experimental design.
3. Matching: is also a non experimental design procedure, is used to control the
extraneous source of variance. In case of controlling organismic and background
variable matching is used in this procedure, the relevant variable are equated or held
constant across all conditions of experiments. Suppose if the researcher finds that the
variable of intelligence is highly correlated with the dependent variable, it is better to
control the variance through matching on the variable of intelligence. However as a
method of control matching limits the availability of subjects. If the researcher decides
to match subjects on two or three variables he may not find enough subjects for the
experiment. Besides this the method of matching biases the principles of randomization.
4. Statistical Control: in this approach, no attempt is made to restrain the influence of
secondary variables. In this technique, one or more concomitant secondary variables
(covariates) are measured and the dependent variable is statistically adjusted to remove
the effect of the uncontrolled sources of variation. Analysis of covariances is one such
technique. It is used to remove statistically the possible amount of variation in the
concomitant secondary variable.
9. ERROR VARIANCE
The third function of a research design is to minimize the error variance. The error variance is
defined as those variance or viabilities in the measures, which occurs as a function of the
factors not controllable by the experimenter. Such factors may be related to the individual
differences among the subjects themselves such as to their attitude, motivation, need, ability
etc. They may be related to what is commonly called the errors of measurements such as the
differences in trials, differences in conditions of experiment, temporary emotional state of the
subject, fatigability etc.
Statistical control can be applied to minimize such error variance. For example,
repeated measures design can be used to minimize the experimental error. By this technique
the variability due to the individual differences is taken out from the total variability, and thus,
the error variance is reduced. Analysis of covariance is also a technique to reduce the error
variance. Further, error variance can be controlled by increasing the reliability of measurement
by giving clear and unambiguously instructions and by using a reliable measuring instrument etc
SOURCES OF ERROR VARIANCE
Poorly designed measurement instruments ( instrumental error)
Error emanating from study subjects ( e.g., response error)
10. Contextual factors that reduce a sound/ accurate measurement instrument’s
capacity to measure accurately
CONTROL TECHNIQUES
Controlling variance of confounding variables:
It can produce undesirable variation in the study’s dependent variables, and
cause misleading or weird results
In Experimental Settings :
1. Conducting the experiment in a controlled environment ( e.g.,
laboratory), where we can hold values of potential confounding
variables constant
2. Subject selection (e.g., matching subjects in experiments)
3. Random assignments of subjects ( variation of confounding variables
are evenly distributed between the experimental and control group )
In Survey Research:
1. Sample selection (e.g., including only subjects with appropriate
characteristics – using male college graduates as subjects will
control for potential confounding effects of gender and
education)
2. Statistical Control
11. CONCLUSION
There are several research designs and the researcher must decide in advance of collection and
analysis of data as to which design would prove to be more appropriate for his research project.
An Effective research design is a function of - 1) Adequate variability in values of research
variables 2) Precise and accurate measurement 3) Identifying and controlling the effects of
confounding variables and 4) Appropriate subject selection
REFERENCE
1. Kerlinger, F N (1986). Foundations of Behavioural Research. New York: Holt Rinehart and
Winston.
2. Thyer, B.A. (1993) ‘ Single-systems Research Design’ in R.M. Grinnell (ed), Social Work,
Research and Evaluation ( 4th ed), Itasca Illionois:Peacock
3. Kothari, C.R. (2004). Research Methodology: Methods and Techniques. New Delhi: New
Age International Publishers.