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Chapter 2
Sexual Reproduction in flowering
plants
Pushpinderjeet Kaur 1
Summary-
• Male reproductive unit
• Female reproductive unit
• Pollination
• Out breeding devices
• Pollen pistil Interaction
• Artificial Hybridisation
• Double fertilisation
• Endosperm
• Embryo development
• Seed & its types
• Fruit formation
• Apomixis
• Polyembryony
Pushpinderjeet Kaur 2
*A flower is a shoot modified for reproduction.
*It contain reproductive organs that forms gametes.
ANDROCEIUM – male gamete—pollen grain
GYNOCEIUM– Female gamete– ova/egg cell
Pushpinderjeet Kaur 3
Parts of Flower
• Calyx (sepal)
• Corolla(petal)
• Androceium
• Gynoceium
• Receptacle
• Peduncle
Pushpinderjeet Kaur 4
Male Reproductive Organ
Structure of anther-
i)Bilobed
ii)Dithecous
iii)4 microsporangia (pollen
sacs)
iv) Tetragonal (4 sided)
•Connective tissue
•Dehiscence of anthers
Pushpinderjeet Kaur 5
Structure of Microsporangia
• Microsporangium is
surrounded by four walls-
1. Epidermis
2. Endothecium protection
3. Middle layers
4. Tapetum- nourishment
Pushpinderjeet Kaur 6
Pushpinderjeet Kaur 7
Pushpinderjeet Kaur 8
POLLEN GRAIN DEVELOPMENT – MICROSPOROGENESIS
Male Gametophyte
Pollen grain can withstand-
• High temperature
• Strong acids and alkali
• Enzymes
Pollen grains are
-decay and chemical resistant
Pushpinderjeet Kaur 9
Structure of pollen grains
Pollen grain has two layers
• Exine- sporopollenin
• Intine- cellulose &pectin
Pollen grain mature and
contain two cells-
• Vegetative cell- food reserve
• Generative cell-forms two
male gametes
Pushpinderjeet Kaur 10
2 celled stage
Pollen grain
• Can cause severe allergies (eg. Parthenium)
• Bronchial afflictions
• Can lead to asthma & bronchitis
Pushpinderjeet Kaur 11
POLLEN PRODUCTS
• Rich in nutrients.
• Increase the performance of athletes (claim).
• Taken in form of tablets & syrups.
Pushpinderjeet Kaur 12
VIABILITY
• Viability of pollen grains depends on temperature & humidity
• Pollen grains can be stored in liquid nitrogen at -196 ˚C in
POLLEN BANKS as part of PLANT BREEDING PROGRAM.
Pushpinderjeet Kaur 13
SPECIES VIABILITY
Rice & Wheat 30 min
Rosaceae, Leguminoseae &
Solanaceae
Months
(Cryopreservation)
Pushpinderjeet Kaur 14
FEMALE REPRODUCTIVE UNIT - CARPEL
Gynoecium
• Female reproductive unit- Stigma , style and
ovary.
• Monocarpellary – Gynoecium with single
pistil.
• Multicarpellary – Gynoecium with more
than one pistil.
• Syncarpous - Fused pistils
• Apocarpous – Free pistils
Pushpinderjeet Kaur 15
Apocarpous ovary
Female Reproductive Unit
Pushpinderjeet Kaur 16
@Sargunpreet Singh
MEGASPORANGIUM
• Ovule attach to placenta by a stalk – Funicle
• Ovlue fuses with funicle at a region – Hilum
• Protective envelopes-Integuments
• A small opening at tip – Micropyle
• The basal part of ouvle, opposite to
micropyle- chalaza
• Mass of cells
with abundant
food reserve –
Nucellus
Pushpinderjeet Kaur 17
MEGASPOROGENESIS
• The process of formation of megaspore from
megaspore mother cell is known as
megasporogenesis.
• Megaspore (ovule) – Is the female
gametophyte.
Pushpinderjeet Kaur 18
Pushpinderjeet Kaur 19
Monosporic development
Free Nuclear Division
Pushpinderjeet Kaur 20
Diagram of a mature embryo sac (8 nucelate 7 celled)
Pushpinderjeet Kaur 21
Pollination
Self
pollination
Autogamy Geitnogamy
Cross
pollination
Xenogamy
*Xenogamy – ensures genetic
recombination
*Cleistogamous flowers are
invariably autogamous.
Pushpinderjeet Kaur 22
• Cleistogamous flowers- bisexual which never open
(commelina,oxalis, viola)
• Assured set of seeds.
Pushpinderjeet Kaur 23
Water pollination
• Water pollination is seen in 30 genera
of mocotyledons.
• Pollens are long , ribbon like
• Female flower stalk is coiled to reach
the water surface to receive the pollen
grains carried by water currents
• Nectar and odour is absent
• Pollen grains are light and unwettable
due to presence of mucilage layer
• Example – vallisneria, Hydrilla, Zostera
Pushpinderjeet Kaur 24
Insect pollination
• Colourful petals
• Nectar
• Fragrant
• Sticky pollen
• Some flower
secrete foul odour
to attract flies and beetles.
Pushpinderjeet Kaur 25
Wind Pollination
• Pollen grains are _______ (sticky/non sticky)
• (heavy/light)
• (dry/wet)
• Stamens are _______ ( exposed/covered)
• Stigma is ____ ( feathery / non – feathery)
Pushpinderjeet Kaur 26
Pushpinderjeet Kaur 27
Yucca plant and Moth
• Moth lays eggs in the locule of the ovary and
flower and inturn get pollinated.
• The larvae of the moth comes out of the ggs
as the seeds starts developing.
Pushpinderjeet Kaur 28

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Reproduction in flowering plants chapter 2

  • 1. Chapter 2 Sexual Reproduction in flowering plants Pushpinderjeet Kaur 1
  • 2. Summary- • Male reproductive unit • Female reproductive unit • Pollination • Out breeding devices • Pollen pistil Interaction • Artificial Hybridisation • Double fertilisation • Endosperm • Embryo development • Seed & its types • Fruit formation • Apomixis • Polyembryony Pushpinderjeet Kaur 2
  • 3. *A flower is a shoot modified for reproduction. *It contain reproductive organs that forms gametes. ANDROCEIUM – male gamete—pollen grain GYNOCEIUM– Female gamete– ova/egg cell Pushpinderjeet Kaur 3
  • 4. Parts of Flower • Calyx (sepal) • Corolla(petal) • Androceium • Gynoceium • Receptacle • Peduncle Pushpinderjeet Kaur 4
  • 5. Male Reproductive Organ Structure of anther- i)Bilobed ii)Dithecous iii)4 microsporangia (pollen sacs) iv) Tetragonal (4 sided) •Connective tissue •Dehiscence of anthers Pushpinderjeet Kaur 5
  • 6. Structure of Microsporangia • Microsporangium is surrounded by four walls- 1. Epidermis 2. Endothecium protection 3. Middle layers 4. Tapetum- nourishment Pushpinderjeet Kaur 6
  • 8. Pushpinderjeet Kaur 8 POLLEN GRAIN DEVELOPMENT – MICROSPOROGENESIS
  • 9. Male Gametophyte Pollen grain can withstand- • High temperature • Strong acids and alkali • Enzymes Pollen grains are -decay and chemical resistant Pushpinderjeet Kaur 9
  • 10. Structure of pollen grains Pollen grain has two layers • Exine- sporopollenin • Intine- cellulose &pectin Pollen grain mature and contain two cells- • Vegetative cell- food reserve • Generative cell-forms two male gametes Pushpinderjeet Kaur 10 2 celled stage
  • 11. Pollen grain • Can cause severe allergies (eg. Parthenium) • Bronchial afflictions • Can lead to asthma & bronchitis Pushpinderjeet Kaur 11
  • 12. POLLEN PRODUCTS • Rich in nutrients. • Increase the performance of athletes (claim). • Taken in form of tablets & syrups. Pushpinderjeet Kaur 12
  • 13. VIABILITY • Viability of pollen grains depends on temperature & humidity • Pollen grains can be stored in liquid nitrogen at -196 ˚C in POLLEN BANKS as part of PLANT BREEDING PROGRAM. Pushpinderjeet Kaur 13 SPECIES VIABILITY Rice & Wheat 30 min Rosaceae, Leguminoseae & Solanaceae Months (Cryopreservation)
  • 14. Pushpinderjeet Kaur 14 FEMALE REPRODUCTIVE UNIT - CARPEL
  • 15. Gynoecium • Female reproductive unit- Stigma , style and ovary. • Monocarpellary – Gynoecium with single pistil. • Multicarpellary – Gynoecium with more than one pistil. • Syncarpous - Fused pistils • Apocarpous – Free pistils Pushpinderjeet Kaur 15 Apocarpous ovary
  • 16. Female Reproductive Unit Pushpinderjeet Kaur 16 @Sargunpreet Singh
  • 17. MEGASPORANGIUM • Ovule attach to placenta by a stalk – Funicle • Ovlue fuses with funicle at a region – Hilum • Protective envelopes-Integuments • A small opening at tip – Micropyle • The basal part of ouvle, opposite to micropyle- chalaza • Mass of cells with abundant food reserve – Nucellus Pushpinderjeet Kaur 17
  • 18. MEGASPOROGENESIS • The process of formation of megaspore from megaspore mother cell is known as megasporogenesis. • Megaspore (ovule) – Is the female gametophyte. Pushpinderjeet Kaur 18
  • 19. Pushpinderjeet Kaur 19 Monosporic development Free Nuclear Division
  • 20. Pushpinderjeet Kaur 20 Diagram of a mature embryo sac (8 nucelate 7 celled)
  • 21. Pushpinderjeet Kaur 21 Pollination Self pollination Autogamy Geitnogamy Cross pollination Xenogamy *Xenogamy – ensures genetic recombination *Cleistogamous flowers are invariably autogamous.
  • 23. • Cleistogamous flowers- bisexual which never open (commelina,oxalis, viola) • Assured set of seeds. Pushpinderjeet Kaur 23
  • 24. Water pollination • Water pollination is seen in 30 genera of mocotyledons. • Pollens are long , ribbon like • Female flower stalk is coiled to reach the water surface to receive the pollen grains carried by water currents • Nectar and odour is absent • Pollen grains are light and unwettable due to presence of mucilage layer • Example – vallisneria, Hydrilla, Zostera Pushpinderjeet Kaur 24
  • 25. Insect pollination • Colourful petals • Nectar • Fragrant • Sticky pollen • Some flower secrete foul odour to attract flies and beetles. Pushpinderjeet Kaur 25
  • 26. Wind Pollination • Pollen grains are _______ (sticky/non sticky) • (heavy/light) • (dry/wet) • Stamens are _______ ( exposed/covered) • Stigma is ____ ( feathery / non – feathery) Pushpinderjeet Kaur 26
  • 28. Yucca plant and Moth • Moth lays eggs in the locule of the ovary and flower and inturn get pollinated. • The larvae of the moth comes out of the ggs as the seeds starts developing. Pushpinderjeet Kaur 28