Allergy, Inflammation, Radiation, Acute Radiation Syndromes, Histamine, Diamine Oxidase, Antiradiation Vaccine, Radiation Toxins, Diagnosis.

1. D M I T R I P O P O V . P H D , R A D I O B I O L O G Y . M D
( R U S S I A )
A D V A N C E D M E D I C A L T E C H N O L O G Y A N D
S Y S T E M S I N C . C A N A D A .
I N T E R V A C C I N E @ G M A I I L . C O M
Rapid Bioassessment Protocol
for Acute Radiation Syndromes.
Histamine Intolerance Test.

2. Radiation: Histamine Intolerance Test.
 Acute Radiation Allergic Reactions (ARAR) is a
mechanism of development of pathophysiological
and clinical sequela after moderate and high doses of
Radiation with a variety of symptoms ranging from
localised rash to life-threatening condition.
 ARAR is a pathological condition resulting from a
Radiation Disease, Radiation Injury, Radiation
Therapy.

3. Radiation:Histamine Intolerance Test.
 Histamine intolerance is due to a (temporary)
 deficiency or an inhibition of the enzyme diamine
 oxidase (DAO). DAO is the enzyme responsible
 for the degradation of histamine. Although DAO
 is virtually found in the whole body, the bowels
 constitute its most important site of action. The
 enzymatic activity of DAO determines the rate
 of degradation of histamine. If a DAO deficiency
 and / or inhibition is present, the organism will not
 be able to degrade quickly enough the histamine
 taken in with food or released from body cells and
 the symptoms of histamine intolerance will occur.

4. Radiation:Histamine Intolerance Test.
 Diamine oxidase (DAO) is a flavoprotein/enzyme
which catalyzes the aerobic oxidation of amines to
the corresponding aldehyde and ammonia.
 Diamine oxidase is synthesized primarily in the
gastrointestinal mucosal cells.
 Serum levels of DAO have been used as an indicator
of the integrity and/or functional mass of the
intestinal mucosa.

5. Radiation:Histamine Intolerance Test.
 Histamine intolerance results from a disequilibrium
of accumulated and activated histamine and the
capacity for histamine degradation.
 Histamine is a biogenic amine that occurs to various
degrees in tissues, blood and lymph as a result of
inflammation or allergic reactions.

6. Radiation:Histamine Intolerance Test.
 Diamine oxidase (DAO) is the main enzyme for the
metabolism of ingested histamine. It has been
proposed that DAO, when functioning as a secretory
protein, may be responsible for scavenging
extracellular histamine after mediator release.
Conversely, histamine N-methyltransferase, the
other important enzyme inactivating histamine, is a
cytosolic protein that can convert histamine only in
the intracellular space of cells.

7. Radiation:Histamine Intolerance Test.
 An impaired histamine degradation based on
reduced DAO activity and the resulting histamine
excess may cause numerous symptoms mimicking
an allergic reaction.
Am J Clin Nutr May 2007 vol. 85 no. 5 1185-1196

8. Radiation : Histamine Intolerance Test.
 Histamine intolerance test can be effective
for Diagnosis of Acute Radiation Syndromes.
 Histamine intolerance, also called histamine
incompatibility, can be detected beyond doubt by
means of a blood test.
 The Histamine intolerance test measures the activity
of the DAO contained in the blood determines the
individual intensity of histamine intolerance makes
possible further bio-assessment selective diagnostics
and therapy recommendations.

9. Radiation : Histamine Intolerance Test.
 In-vitro diagnostic procedure for quantitative
determination of diamine oxidase activity in
the serum.
 The test is based on a radio immuno assay (RIA) or ELISA
principles. The determination of the DAO activity is achieved
by determining the concentration of a reaction product.
Radiolabelled putrescine is used as substrate. The reaction
product is radioactive 1-pyrroline that is transferred into an
organic solvent by means of liquid phase extraction.
 After adding scintillator, radioactivity is determined by a beta
counter. The quantity of radioactivity measured is directly
proportional to the DAO activity of the sample.

10. Radiation : Histamine Intolerance Test.
 The histamine intolerance test measures the activity
of the DAO contained in the blood, since the DAO
circulates through the body in the blood. The blood
is mixed with a substance similar to histamine
and the degradation of this substance is measured.
The larger the quantity of DAO in the blood, the
larger is the portion of the substance that is
degraded. For Biological assessment of doses
radiation levels of Histamine and DAO activity
should be measured.

11. Radiation : Histamine Intolerance Test.
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