Radiation biology is the study of the effects of ionizing radiation on living systems. Radiation interacts with matter through Compton and photoelectric processes, producing free radicals that can damage biological molecules like DNA, proteins and lipids. This leads to cellular effects like chromosomal aberrations, cell death, and changes in cell kinetics. The severity of radiation effects depends on factors like dose, dose rate, cell type, oxygen levels and time since exposure. In oral tissues, radiation can cause mucositis, xerostomia due to salivary gland damage, taste loss, tooth development issues, and rampant dental caries.
ORN is an inflammatory condition of bone that occurs after the bone has been exposed to therapeutic doses of radiation usually given for a malignancies.
ORN is an inflammatory condition of bone that occurs after the bone has been exposed to therapeutic doses of radiation usually given for a malignancies.
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Radiobiology (also known as radiation biology, and uncommonly as actinobiology) is a field of clinical and basic medical sciences that involves the study of the action of ionizing radiation on living things, especially health effects of radiation.
The action is very complex, involving physics, chemistry, and biology
– Different types of ionizing radiation
– Energy absorption at the atomic and molecular level
leads to biological damage
– Repair of damage in living organisms
RADIATION BIOLOGY- ORAL MEDICINE AND RADIOLOGYeducarenaac
A major theme for the radiobiology section is the use of radiation as a model agent to study cellular responses including genomic instability, cell cycle controls, DNA damage processing, oxidative stress, senescence, and apoptosis, as well as the signaling mechanisms mediating these and other stress responses.
One of the most developed cities of India, the city of Chennai is the capital of Tamilnadu and many people from different parts of India come here to earn their bread and butter. Being a metropolitan, the city is filled with towering building and beaches but the sad part as with almost every Indian city
Defecation
Normal defecation begins with movement in the left colon, moving stool toward the anus. When stool reaches the rectum, the distention causes relaxation of the internal sphincter and an awareness of the need to defecate. At the time of defecation, the external sphincter relaxes, and abdominal muscles contract, increasing intrarectal pressure and forcing the stool out
The Valsalva maneuver exerts pressure to expel faeces through a voluntary contraction of the abdominal muscles while maintaining forced expiration against a closed airway. Patients with cardiovascular disease, glaucoma, increased intracranial pressure, or a new surgical wound are at greater risk for cardiac dysrhythmias and elevated blood pressure with the Valsalva maneuver and need to avoid straining to pass the stool.
Normal defecation is painless, resulting in passage of soft, formed stool
CONSTIPATION
Constipation is a symptom, not a disease. Improper diet, reduced fluid intake, lack of exercise, and certain medications can cause constipation. For example, patients receiving opiates for pain after surgery often require a stool softener or laxative to prevent constipation. The signs of constipation include infrequent bowel movements (less than every 3 days), difficulty passing stools, excessive straining, inability to defecate at will, and hard feaces
IMPACTION
Fecal impaction results from unrelieved constipation. It is a collection of hardened feces wedged in the rectum that a person cannot expel. In cases of severe impaction the mass extends up into the sigmoid colon.
DIARRHEA
Diarrhea is an increase in the number of stools and the passage of liquid, unformed feces. It is associated with disorders affecting digestion, absorption, and secretion in the GI tract. Intestinal contents pass through the small and large intestine too quickly to allow for the usual absorption of fluid and nutrients. Irritation within the colon results in increased mucus secretion. As a result, feces become watery, and the patient is unable to control the urge to defecate. Normally an anal bag is safe and effective in long-term treatment of patients with fecal incontinence at home, in hospice, or in the hospital. Fecal incontinence is expensive and a potentially dangerous condition in terms of contamination and risk of skin ulceration
HEMORRHOIDS
Hemorrhoids are dilated, engorged veins in the lining of the rectum. They are either external or internal.
FLATULENCE
As gas accumulates in the lumen of the intestines, the bowel wall stretches and distends (flatulence). It is a common cause of abdominal fullness, pain, and cramping. Normally intestinal gas escapes through the mouth (belching) or the anus (passing of flatus)
FECAL INCONTINENCE
Fecal incontinence is the inability to control passage of feces and gas from the anus. Incontinence harms a patient’s body image
PREPARATION AND GIVING OF LAXATIVESACCORDING TO POTTER AND PERRY,
An enema is the instillation of a solution into the rectum and sig
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Antibiotic Stewardship by Anushri Srivastava.pptxAnushriSrivastav
Stewardship is the act of taking good care of something.
Antimicrobial stewardship is a coordinated program that promotes the appropriate use of antimicrobials (including antibiotics), improves patient outcomes, reduces microbial resistance, and decreases the spread of infections caused by multidrug-resistant organisms.
WHO launched the Global Antimicrobial Resistance and Use Surveillance System (GLASS) in 2015 to fill knowledge gaps and inform strategies at all levels.
ACCORDING TO apic.org,
Antimicrobial stewardship is a coordinated program that promotes the appropriate use of antimicrobials (including antibiotics), improves patient outcomes, reduces microbial resistance, and decreases the spread of infections caused by multidrug-resistant organisms.
ACCORDING TO pewtrusts.org,
Antibiotic stewardship refers to efforts in doctors’ offices, hospitals, long term care facilities, and other health care settings to ensure that antibiotics are used only when necessary and appropriate
According to WHO,
Antimicrobial stewardship is a systematic approach to educate and support health care professionals to follow evidence-based guidelines for prescribing and administering antimicrobials
In 1996, John McGowan and Dale Gerding first applied the term antimicrobial stewardship, where they suggested a causal association between antimicrobial agent use and resistance. They also focused on the urgency of large-scale controlled trials of antimicrobial-use regulation employing sophisticated epidemiologic methods, molecular typing, and precise resistance mechanism analysis.
Antimicrobial Stewardship(AMS) refers to the optimal selection, dosing, and duration of antimicrobial treatment resulting in the best clinical outcome with minimal side effects to the patients and minimal impact on subsequent resistance.
According to the 2019 report, in the US, more than 2.8 million antibiotic-resistant infections occur each year, and more than 35000 people die. In addition to this, it also mentioned that 223,900 cases of Clostridoides difficile occurred in 2017, of which 12800 people died. The report did not include viruses or parasites
VISION
Being proactive
Supporting optimal animal and human health
Exploring ways to reduce overall use of antimicrobials
Using the drugs that prevent and treat disease by killing microscopic organisms in a responsible way
GOAL
to prevent the generation and spread of antimicrobial resistance (AMR). Doing so will preserve the effectiveness of these drugs in animals and humans for years to come.
being to preserve human and animal health and the effectiveness of antimicrobial medications.
to implement a multidisciplinary approach in assembling a stewardship team to include an infectious disease physician, a clinical pharmacist with infectious diseases training, infection preventionist, and a close collaboration with the staff in the clinical microbiology laboratory
to prevent antimicrobial overuse, misuse and abuse.
to minimize the developme
Empowering ACOs: Leveraging Quality Management Tools for MIPS and BeyondHealth Catalyst
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In this session, we will explore how a robust quality management solution can empower your organization to meet regulatory requirements and improve processes for MIPS reporting and internal quality programs. Learn how our MeasureAble application enables compliance and fosters continuous improvement.
India Clinical Trials Market: Industry Size and Growth Trends [2030] Analyzed...Kumar Satyam
According to TechSci Research report, "India Clinical Trials Market- By Region, Competition, Forecast & Opportunities, 2030F," the India Clinical Trials Market was valued at USD 2.05 billion in 2024 and is projected to grow at a compound annual growth rate (CAGR) of 8.64% through 2030. The market is driven by a variety of factors, making India an attractive destination for pharmaceutical companies and researchers. India's vast and diverse patient population, cost-effective operational environment, and a large pool of skilled medical professionals contribute significantly to the market's growth. Additionally, increasing government support in streamlining regulations and the growing prevalence of lifestyle diseases further propel the clinical trials market.
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3. HISTORY
✓ First recorded experiment in radiobiology -
performed by Becquerel when he intentionally left
a radium container in his vest pocket.
2 weeks later – erythema - ulceration - several
weeks to heal
✓ Pierre Curie – 1901 – repeated the experiment –
radium burn on his forearm
5. RADIATION INTERACT WITH
MATTER : COMPTON
PROCESS AND
PHOTOELECTRIC
PROCESS
EFFECT
What does radiation do inside the body ?
PHOTONS
INTERACTION WITH
BIOLOGICAL
MOLECULES
FREE RADICAL
PRODUCTION
6. How do X-rays cause damage ?
Ionization – is a process where an atom or
group of atoms loses one or more
electrons
8. RADIATION
INTERACT WITH
MATTER : COMPTON
PROCESS AND
PHOTOELECTRIC
PROCESS
FREE RADICAL
PRODUCTION
PHOTONS
INTERACTION
WITH
BIOLOGICAL
MOLECULES
EFFECT
- The rate of loss of energy from a
particle as it moves along its track
through matter is its linear energy
transfer (LET)
- Radiations with high LET have higher
tendency for ionization à severe
damage
9. - The initial interaction between ionizing radiation and
matter occurs at the level of the electron within first
10-13 sec after exposure
- The generation of free radicals occurs in less than 10-10
sec after the passage of a photon
13. 1. Direct or Target Action Theory (1/3rd)
2. Indirect Action or Poison Chemical
Theory (2/3rd)
Interaction of radiation
with biological
molecules
RADIATION
INTERACT WITH
MATTER : COMPTON
PROCESS AND
PHOTOELECTRIC
PROCESS
FREE RADICAL
PRODUCTION
PHOTONS
INTERACTION
WITH
BIOLOGICAL
MOLECULES
EFFECT
14. ▪A secondary electron resulting
from absorption of an x-ray photon
interacts with DNA directly by
breaking the relatively weak bonds
between nucleic acids to produce
the effect
RH + x-radiation à R. + H+ + e
R. à X + Y.
R. + S. àRS
- Results in altered molecule that differs structurally and
functionally from the original molecule
Direct action
16. - Sparsely ionizing radiations such as x-rays or gamma- rays
induce damage mainly through indirect effect while densely
ionizing radiations such as neutrons and alpha particles cause
damage primarily through direct effect
- Indirect effects can be altered with use of radioprotectors
during radiotherapy while direct not
17. ▪Proteins
▪Denaturation
▪Induce Inter & intra-molecular cross-linking
▪ Disruption of secondary and tertiary structure
▪Nucleic acids
▪Primary mechanism of radiation induced cell death
▪Change or loss of base
▪Disruption of hydrogen bonds between DNA strands
▪Breakage of DNA strands
▪Cross-linking of DNA strands
Effect on biological molecules
18. ▪Intracellular
▪Nucleus – most sensitive; inhibition of cell division
▪Cell cytoplasm – increased permeability to Na & K ions,
swelling & disorganization of mitochondria, focal
cytoplasmic necrosis
▪Telomerase is produced – cell becomes immortal
▪Chromosomes – single or double armed chromosomal
aberrations
Effects at cellular levels
19. ▪Chromosome aberrations – irradiated early in interphase,
before the chromosome material has duplicated – break in
both chromatids strands
▪Chromatid aberrations – irradiated later in interphase,
after DNA material has doubled – break seen in one
chromatid strand
Aberrations
23. CELL CYCLE CHECK POINTS
-To prevent progression of
defective DNA through cell
cycle
- two check points – G1S and
G2M
24. CELL CYCLE CHECK POINTS
CHECK POINT
G 1 - S C H E C K
POINT
-Before a cell makes the final commitment to
replicate, the G1/S checkpoint checks for DNA
damage;
- if damage is present, the DNA-repair machinery
and mechanisms that arrest the cell cycle
- The delay in cell cycle progression provides the
time needed for DNA repair;
- if the damage is not repairable, apoptotic pathways
are activated to kill the cell.
25. G2-M CHECK
POINT
-The G2/M checkpoint monitors the completion
of DNA replication and checks whether the cell
can safely initiate mitosis and separate sister
chromatids.
- This checkpoint is particularly important in
cells exposed to ionizing radiation.
- Cells damaged by ionizing radiation activate
the G2/M checkpoint and arrest in G2; defects in
this checkpoint give rise to chromosomal
abnormalities
26. ▪Enzymatic repair of single-stranded breaks of DNA
▪Damage to both strands: lethal to cell.
Recovery:
27. - Radiation affects the expression
of both cyclins and cyclin
dependent kinases
- Delay in cellular progression
through cell division
Mitotic delay
29. ▪Cell death can occur by two ways
1. Mitotic death
2. apoptosis
Cell death
30. 1. Mitotic death:
- Also called reproductive death
- dominant mode of cell killing
- cell dies when they attempt to divide
- double strand breakage in DNA frequent cause of
cell death
- Mean lethal dose for loss of proliferative capacity - 2Gy
Cell death
31. 2. Apoptosis:
- Typically observe after low radiation dose
- occurs within hours after radiation exposure
- seen in lymphomas , serous cells of salivary
glands
- lead to rapid onset xerostomia after moderate
dose of radiations
Cell death
34. ▪Different cells from various organs of the same
individual may respond to irradiation quite differently
▪This variation was recognized as early as 1906 by
the French radiobiologists and is called “Law of
Bergonie and Tribondeau”.
▪“Radiation is more effective against cells that are
actively dividing, are undifferentiated and have
large dividing future”
RADIOSENSITIVITY AND CELL TYPE
35. ▪They observed that the most radiosensitive cells are
those that
(1) Have a high mitotic rate
(2) Undergo many future mitoses
(3) Are most primitive in differentiation
▪Lymphocytes and oocytes: exceptional
36. ▪MODIFIED BY ANCEL AND VITEMBERGER
▪The appearance of radiation damage is dependent
on 2 factors
▪Biologic stress on the cell (most important
biologic stress— cell division— rapidly dividing
cells express damage earlier; slowly dividing cells
express later)
▪Conditions to which the cell is exposed pre and
post irradiation
RADIOSENSITIVITY AND CELL TYPE
37. ▪Classification of cellular populations based on
reproductive kinetics into five categories
▪To explain the difference in observed cellular and tissue
radiosensitivity based on the reproductive and functional
characteristics of various cell lines
RUBIN AND CASARETT
CLASSIFICATION
38. Vegetative intermitotic
cells
-Most radiosensitive
-Primitive in differentiation
-High mitotic rate
-E.g. cells of spermatogenic and
erythroblastic series, basal cells
of oral mucous membrane.
Differentiating
intermitotic cells
-Less radiosensitive than
vegetative cells
-Divide regularly
-Undergo some differentiation
between divisions
-E.g. inner enamel epithelium of
developing teeth, cells of
haematopoietic series,
spermatocytes and oocytes.
39. Multi potential
connective tissue cells
-Have intermediate sensitivity
-Divide when there is demand
for more cells
-E.g.endothelial cells,
fibroblasts, mesenchymal cells
Reverting post mitotic
cells
-Radio resistant because they
divide infrequently
- e.g. acinar, ductal,
parenchymal cells of glands.
Fixed post mitotic cells -Most resistant
-- highly differentiated cells
--incapable of division
-- e.g. neurons, striated muscle
cells, epithelial cells,
erythrocytes.
40. H-TYPE populations
▪Tissues with hierarchial organisation : include bone marrow,
intestinal epithelium, epidermis and many others
▪Basal layer of proliferating stem cells and superficial layers of non
proliferating functional cells
▪Radiation injury appears at a predictable time determined by life
span of mature cells ; when they are depleted by normal
physiological wear and tear
▪E.g. radiation mucositis appears 2 weeks after onset of RT
Michalowski Classification
41. ▪Flexible tissues
▪Composed of functional parenchyma
▪Has very low turn over rate
▪Flexible: Regain reproductive function in event of tissue
loss
▪E.g. bone , soft tissue , endocrine tissue
▪Fibrosis of tissues following RT
F-type populations
43. 1. Somatic & Genetic Effects
2. Stochastic & Deterministic Effects
3. Short - term & Long - term Effects
Classification of
biologic effects of
radiation
RADIATION
INTERACT WITH
MATTER : COMPTON
PROCESS AND
PHOTOELECTRIC
PROCESS
FREE RADICAL
PRODUCTION
PHOTONS
INTERACTION
WITH
BIOLOGICAL
MOLECULES
EFFECT
44. 1. Somatic vs. Genetic Effects
Definition Effects Examples
Somatic Harm to person
exposed to the
radiation
Acute — large dose of
radiation absorbed in a
short time
Chronic — small amt of
radiations absorbed
repeatedly over long
time (latent period ≥20
yrs)
- Leukemia
Genetic Harm to person
in future
generation due
to ancestor’s
exposure
Radiation to reproductive
organs damage the DNA
of sperms or eggs
No threshold dose
- Congenital
abnormality
46. 2. Stochastic vs. Deterministic Effects
Traits Effects Example
Stochastic “all or none”
response; any
dose can cause
No threshold dose
Every exposure to
ionizing radiation
carries with it the
possibility of
inducing a
stochastic effect.
é Incidence of
leukaemia &
certain tumours
Deterministi
c
(non
stochastic)
“certainty effects”;
severity of effect
é with é
dose;
Threshold dose
exists below which
there will be no
effect.
Erythema: all will
experience once
minimum dose is
given; gets
worse with
édose
47. 3. Short - term vs. Long - term Effects
Traits Example
Short term
effects
Determined primarily
by sensitivity of its
parenchymal cells
Bone marrow, oral mucosa
- high radiosensitive -
mitosis-linked cell death.
Muscle - low radiosensitive
Long term
effects
Depend primarily on
extent of damage to
fine vasculature
Progressive
fibroatrophy of the
irradiated tissue
49. DOSE
▪The severity of deterministic damage seen in irradiated
tissues or organs depends on the amount of radiation
received
▪All individuals receiving doses above the threshold level
show damage in proportion to the dose
FACTORS : MODIFY EFFECT OF
RADIATION
50. Dose Rate
▪High dose rate causes more
damage than exposure to the
same total dose given at a
lower dose rate
▪When organisms are exposed
at lower dose rates, a greater
opportunity exists for repair of
damage, thereby resulting in
less net damage
51. Oxygen
▪Presence of oxygen alters
how cells process free
radicals
▪In presence of oxygen free
radicals lead to irreparable
lethal changes
52. OXYGEN ENHANCEMENT RATIO:
• magnitude of the effect of oxygen on radiosensitivity
• Ratio of hypoxic to aerated cells to achieve the same
biologic effect.
• Sparsely ionizing radiation: 2.5-3
53. Cell age in division cycle
▪Cells in late G2 – most radiosensitive
▪S phase – radioresistant
▪Late G1 and early S- intermediate sensitivity
54. ▪Higher-LET radiations (α particles) are more efficient in
damaging biologic systems because their high ionization
density is more likely than x rays to induce double strand
breakage in DNA
Linear Energy Transfer
57. Oral Mucous Membrane
Basal layer - vegetative and
differentiating inter-mitotic
cells (radiosensitive)
2nd week after therapy-
redness and Inflammation
(Mucositis)
58. PATHOPHYSIOLOGY OF ORAL MUCOSITIS
CELL EXPOSUR E
TO RADIOTHERAPY
STIMULATE
TRANSCRIPTION
FACTORS
INGRESS OF
BACTERIA, VIRUS,
FUNGI
TISSUE
INJURY
CELLULAR
PROLIFERTION
GENERATION OF
REACTIVE
OXYGEN
SPECIES
ULCERATION
DNA
DAMAGE
CYTOKINES
TISSUE
HEALING
59. ▪Radiation induces fatty degeneration, fibrosis, acinar
atrophy and cellular necrosis within the glands
▪Serous acini are more radiosensitive
▪Reduced saliva, thick and ropy
▪Xerostomia
Salivary Glands
60. ▪Viscous saliva
▪ decrease salivary flow
▪pH on unit less = 5.5
▪buffering capacity decreases by 44%
▪Irreversible effects occur at a total dose of 6,000 cGy for 5
weeks
▪Management
Frequent sips of water, sugarless chewing gum,
pilocarpine hydrochloride (5mg – 4 times a day),
Bethanechol 25-200mg/day
Salivary Glands
61. ▪Partial or complete loss of taste if taste buds are irradiated
▪Irradiation of posterior tongue à loss of bitter and acid
flavours
▪Irradiation of anterior tongue à loss of sweet and salt
flavours
▪Generally cells regenerate in 4 months
Management
Dietary consultation-improve quality of food
Zinc sulphate capsules- 220mg twice daily with food,
elemental zinc- 100mg
Effects on Taste Buds
62. Radiation effect on teeth
• Before calcification: Destroys tooth bud
• After calcification : inhibits cellular differentiation à
malformation and arrested growth
• During development: Retards growth
• Eruptive Mechanism:Radioresistant
• In adults : No effect on calcified tissue
• Pulp: Fibroatrophy
63. ▪Radiation caries is a rampant form of dental decay
that may occur in individuals who receive a course
of radiotherapy that includes exposure of the
salivary glands
▪The carious lesions result from changes in the
salivary glands and saliva, including reduced flow,
decreased pH, reduced buffering capacity, and
increased viscosity
Radiation Caries
64. ▪Because of the reduced or absent cleansing action
of normal saliva, debris accumulates quickly
▪Irradiation of the teeth by itself does not influence
the course of radiation caries
65. ▪Clinically, three types of
radiation caries exist
▪The most common is
widespread superficial
lesions attacking
buccal, occlusal, incisal,
and palatal surfaces
66. ▪Another type involves
primarily the cementum &
dentin in the cervical region
▪These lesions may progress
around the teeth
circumferentially and result
in loss of the crown
67. ▪A final type appears
as a dark
pigmentation of the
entire crown
▪The incisal edges
may be markedly
worn
Management
Hydrogen peroxide rinses 3% (maintenance of oral
hygiene)
Topical fluoride applications
68. MUSCLES & JOINTS
▪ Trismus may develop due to tumour invasion of the masticatory
muscles and/or the TMJ or
▪ be the result of radiotherapy if masticatory muscles and/or the TMJ
is included in the field of radiation, or a combination of both
69. ▪Trismus develops 3-6 mths after radiation treatment is
completed and frequently becomes a lifelong problem
Ichimura and Tanaka, 1993
▪Trismus is attributed to muscle fibrosis and scarring in
response to radiation injury as well as to fibrosis of the
ligaments around the TMJ and scarring of the
pterygomandibular raphes
70. OSTEORADIONECROSIS
-Also known as post radiation osteonecrosis (PRON)
- first described by – Regaud in 1920
- a serious deforming , debilitating complication of radiation
therapy
71. PATHOPHYSIOLOGY:
1. Meyer in 1970:
- triad of radiation , trauma and infection
- Irradiated bone à traumatic event à ingress of micro-
organisms à osteoradionecrosis
Failure :
- Failure to demonstrate bacterial invasion in compromised
bone
- ORN can occur without any definable traumatic event
- poor response to treatment with antibiotic therapy
72. PATHOPHYSIOLOGY:
2. Marx in 1983- “three H principle”
- Cumulative tissue damage induced by radiation rather than
trauma or bacterial invasion à hypocellular, hypovascular
and hypoxic tissue
73. 3. Fibroatrophic theory :
- Introduced in 2004
- Key event in progression of ORN is activation and
dysregulation of fibroblastic activity à atrophic tissue
74.
75. Three distinct phases :
1. Prefibrotic phase : acute inflammatory response à changes
in endothelial cells
2. Constitutive organized phase : abnormal fibroblastic activity
à disorganization of ECM
3. Late fibroatrophic phase: attempted tissue remodelling
àfragile tissue ; carries inherent risk of reactivation of
inflammation secondary to local trauma
76. -In head and neck region commonest site is mandible > maxilla>
temporal bone > sphenoid bone> base of skull
Signs and symptoms :
-Pain
-Swelling
-Trismus
-Halitosis
- exposed bone
- pathological fracture
- oro- cutaneous fistula
79. PREVENTION OF ORN:
-Recommendations given by Sleeper and Meyer
1. Mouth should be made as clean as possible with scaling and
irrigation
2. All infections should be eliminated
3. All infected and non vital teeth should be extracted
4. All periodontally compromised teeth should be extracted
5. all teeth in line of irradiation (good or bad)
80. MANAGEMENT OF OSTEORADIONECROSIS:
-Control of infection if present
- remove debris and gentle irrigation
- supportive treatment with fluids + diet high in
protein
- pain management – narcotic analgesics , nerve
blocks, nerve avulsion
- hyperbaric oxygen therapy
81. HYPERBARIC OXYGEN THERAPY :
-Consist of exposing a patient to intermittent short
term 100% oxygen inhalation at a pressure greater than
1 atmosphere
- HBO à induce endothelial cell proliferation à
neovascularization
Contraindications:
-Untreated pneumothorax
- pregnancy
- emphysema
- URTI, optic neuritis , ear problems
83. Whole body radiation
▪Acute Radiation Syndrome: The acute radiation
syndrome is a collection of signs and symptoms
experienced by individuals after a brief whole-body
exposure to radiation
Dose in Gy Manifestations
1-2 Prodromal symptoms
2-4 Mild hematopoietic symptoms
4-7 Severe hematopoietic symptoms
7-15 Gastrointestinal symptoms
>50 Cardiovascular and CNS
symptoms
84. ▪Prodromal symptoms
-Minutes to hours after exposure
-Anorexia, nausea, vomiting, diarrhea,
fatigue and weakness
- Higher the dose , the more rapid the
onset and greater will be severity of
symptoms
▪Latent period
extends from hours to days
patient is apparently normal
Acute Radiation Syndrome
85. • Radiation causes damage to hematopoietic cells of
bone marrow & spleen
• Remarkable fall in the no. of granulocytes, platelets
and erythrocytes
• Clinical signs: Infection, hemorrhage & anemia
• Death may occur 10 to 30 days after radiation
• Management: removal of sources of infection,
antibiotics and bone marrow transplantation
Hematopoietic Syndrome
86. ▪Rapidly proliferating basal epithelial cells of the intestinal
villi are damaged
▪Loss of epithelial layer of the intestinal mucosa àloss of
plasma & electrolytes
▪Reduced efficiency of intestinal absorption
▪Ulceration and hemorrhage in the intestines
▪Bacteria invade denuded areasà septicemia
▪diarrhea, dehydration, loss of weight
▪Death occurs within 2 weeks : combination of fluid and
electrolyte loss, infection and nutritional impairement
GI Syndrome
87. ▪Irreversible condition
▪> 50 Gy causes death in 1- 2 days
▪Circulatory system collapses, drastic fall in BP
▪Incoordination, disorientation, and
convulsions
CVS and CNS syndrome
90. 1. Skin –
i. Early or acute signs
▪ Increased susceptibility to chapping
▪ Intolerance to surgical scrub
▪ Blunting & leveling of finger ridges
▪ Brittleness & ridging of finger nails
ii. Late or chronic signs
▪ Loss of hair
▪ Dryness & atrophy of skin – sweat glands destroyed
▪ Pigmentations, telangiectasis, keratosis
▪ Indolent type of ulcerations
▪ Possibility of malignant changes in tissue
General effects of radiation
91. 2. Hematopoietic injury – leucopenia, which in some
cases may progress to leukemia, anemia, lymphopenia
3. Eyes
▪ Epilation of eyelashes
▪ Inflammation, fibrosis, & decreased flexibility of eye
lids
▪ Dryness
▪ Ulceration & cataract
92. 4. Ears
▪edema of mucosa & collection of fluid causing
obstruction of Eustachian tube – Radiation Otitis
Media
▪Deafness due to rupture of ear drum
5. Testicles
▪ Suppression of germinal activity
▪ Alteration in fertility
94. 8. Taste buds – alterations in taste
9. Salivary glands – parenchymal component
affected – fibrosis, adiposis, loss of fine
vasculature.
▪ Xerostomia
▪ Composition of saliva altered
▪ Increased Na, Cl, Ca, Mg & proteins
▪ Loses lubricating property
▪ PH decreases – decalcification of enamel
▪ Compensatory gland hypertrophy
95. 10.Teeth
▪ Adult teeth are resistant to radiation
▪ Prior to calcification tooth buds destroy
▪ During calcification defect in cellular
differentiation - ↓sed vascularity, cellularity of
pulp & prone to pulpitis
▪ Radiation caries
96. 11. Bone
▪ Loss of vasculature & hematopoietic
elements
▪ Marrow replaced by fatty marrow & fibrous
connective tissue
▪ Lack of osteoblasts & osteoblastic activity
▪ Osteoradionecrosis following radiation
97. 12.Whole body radiation
▪ Acute radiation syndrome – death within one month
▪ Late somatic effects –
▪ radiation induced cancers
99. ▪Radiation leads to modification of biologic
molecules
▪Molecular changes may lead to alteration in
cells and organisms
▪If enough cells are killed à injury or death
▪ if cells are modified à cancer
100. High Intermediate Low
- Colon
- Stomach
- lung
- bone marrow
(leukemia)
- Breast
(women)
- Esophagus
- Bladder
- Liver
- Thyroid
- Skin
- Bone surface
- Brain
- Salivary
glands
Susceptibility of different tissues to radiation induced cancer
101. ANGIOGENESIS
ALTERATION IN GENES
THAT REGULATE
APOPTOSIS
INACTIVATION OF
TUMOR SUPRESSOR
GENES
ACTIVATION OF
GROWTH PROMOTING
GENES
Mutations in the genome of somatic cell
Normal cell
DNA Damage
Failure of DNA repair
Clonal expansion
Decreased apoptosisUnregulated cell proliferation
Invasion and metastasis
Malignant neoplasm
Tumor progression
SUCCESSFUL
DNA REPAIR
ADDITIONAL MUTATIONS
ESCAPE FROM IMMUNITY
102. - Radiation induced cancers are indistinguishable
from cancers produce by other causes
- Risk is greater in childhood
- Most cancers appear approximately 10yrs after
exposure
- Risk of leukemia is higher than solid tumors ;
higher rate of cell division and differentiation of
hematopoietic stem cells
103. EFFECT ON GROWTH AND DEVELOPMENT
▪children exposed to radiation à reduced height,
weight, skeletal development
▪ developing human brain is radiosensitive : in
utero exposure à mental retardation (4%
chance of mental retardation per 100mSv at 8 to
15 weeks of gestation)
104. EFFECTS ON FETUS & UTERUS
▪Preimplantation:
dose of 0.2Gy à death of embryo
▪Organogenesis:
congenital anomalies
▪The fetal period:
Largest degree of growth retardation.
105. ▪The amount of radiation a population requires
to produce in the next generation as many
additional mutation as arise spontaneously
▪In humans : approx 2Gy
DOUBLING DOSE
107. ▪Provides greater tumour destruction than is possible
with a large single dose
▪Increased cellular repair of normal tissue
▪Increases the mean oxygen tension in an irradiated
tumour
RATIONALE OF FRACTIONATION IN
RADIOTHERAPY
108. ▪Repair of sublethal damage in normal cells
▪Reassortment of cells within the cell cycle
▪Repopulation of normal cells
▪Reoxygenation of tumour bed
4 R’s of Radiobiology
109. BIOLOGIC BASIS OF DOSE FRACTIONATION
1. REPAIR OF SUBLETHAL DAMAGE:
- Reflects the ability of cells to recover from damage that does
not cause lethality
- Repair capacity is greater in late responding tissues
- decreasing the dose per fraction will result in greater sparing
of late responding tissues
110. Cell survival curve
Curve describes the relationship
between the radiation dose & the
proportion of cells that survive
Mean lethal dose kills on average
63% of cells
Accumulation of sublethal
damage
Each additional dose reduces cell
survival to 37% of the previous
level
111. 2. REDISTRIBUTION:
- First radiation dose preferentially kills cells in the sensitive
phases
- cells surviving the first dose will resume their progression
through cell cycle into more radiosensitive phases
- Resulting in net sensitization to the next dose fraction
- redistribution results in net gain in therapeutic ratio
112. 3. REGENERATION :
- Depletion of cells following radiation or surgery triggers a
regenerative response , resulting in net increase in cell
production
- decrease normal tissue injury
113. 4. REOXYGENATION :
- Hypoxic cells are radioresistant
- Preferential elimination of more radiosensitive oxygenated
cells increases oxygen availability to surviving hypoxic cells
- increase tissue sensitivity to radiation
114. According to standard therapeutic regimen :
Dose of 200cGY is delivered ; 5 days a week
for 5-6 weeks with total dosage of 50-60 Gy
115. ALTERED FRACTIONATION SCHEMES
1. HYPERFRACTIONATION :
- Smaller fraction size ( 115-120cGy)
- Two to three times in a day
- total dosage is larger ( 74-80Gy)
- treatment duration is same
Advantages : improve therapeutic ratio
- Redistribution of tumor cells into more radiosensitive phases
due to multiple fractions
- differential sparing of late responding normal tissue
116. ALTERED FRACTIONATION SCHEMES
2. ACCELERATED FRACTIONATION :
- Same fraction size ( 180-200cGy)
- Two to three times in a day
- total dosage is similar
- treatment duration is short
Advantages :
- reduce the opportunity for accelerated repopulation of cells
117. 3. HYPOFRACTIONATION :
- Larger fraction size ( 600-800cGy)
- Fractions delivered with gap of several days
- total dosage is lower (2100-3200cGy)
- treatment duration is short
Advantages :
- Evolved for the treatment of malignant melanoma ( conventional
fractions allow tumor cells to recover between fraction
intervals , high dose per fraction will overcome the reparative
capacity of tumor cells
ALTERED FRACTIONATION SCHEMES
119. RADIOSENSITIZATION
REDUCING HYPOXIA
- Correction of anemia
- Infusion of erythropoietin
- Hyperbaric oxygen therapy
CONCOMITANT CHEMOTHERAPY
- Decrease micrometastasis
- Kill hypoxic cells ( mitomycin active in hypoxic condition )
- Kills radioresistant cells in S phase of cell cycle
120. RADIOPROTECTORS
Agents that when present prior to or shortly after radiation
exposure alter the response of normal tissues to irradiation.
- Sulfhydryl compounds such as cysteine and cysteamine have
long been known to act as radioprotectors via free radical
scavenging à Alter the indirect effect of radiation à
protection of normal tissue
121. SUMMARY
The toxic biological effects of ionizing radiation, although complex,
varied,and incompletely understood, form the basis for the use of
radiation therapy as a cancer treatment. These biological effects are
initiated when packets of energy are deposited in a volume of tissue and
remove electrons from constituent atoms through a process called
ionization. Accordingly, the physics of radiation oncology is focused on
the details of how, where, and how much energy can be deposited in
diseased tissue in the hopes of eradicating it, while simultaneously
minimizing the energy released in healthy tissue. This process requires
an understanding of the nature of the radiation and the matter through
which it passes and how that matter is changed as a result of the energy
deposition events.
122. 1. Oral radiology, principles & interpretation: white & pharoah
2. Essentials of dental radiography & radiology: whaites
3. Oral sequelae of head & neck radiotherapy: crit rev oral biol med 2003
4. Prevention & treatment of the consequences of head & neck radiotherapy:
crit rev oral biol med 2003
5. Dental management of the oncologic patient: DCNA 2008
6. Protocol for the prevention and treatment of oral sequelae resulting from
head and neck radiation therapy: cancer 1992
REFERENCES
124. ADVANCES IN RADIOTHERAPY
ALTERED
FRACTIONATION
• HYPOFRACTIONATION
•
HYPERFRACTIONATIO
N
• ACCELERATED
FRACTIONATION
- USE OF HEAVILY
CHARGED PARTICLES
- NEUTRONS ARE
USED CAUSING MORE
CELL DESTRUCTION
- Conformational
radiotherapy
• multi leaf
collimator
• 3D -CRT
125. CONFORMATIONAL RADIOTHERAPY
1. MULTI-LEAF COLLIMATOR:
- Enables flexible portal shaping
- achieve desired dose distribution with in target volume
( field- in field technique )
126. CONFORMATIONAL RADIOTHERAPY
2. 3D CRT / 3 DIMENSIONAL CONFORAMTIONAL RADIOTHERAPY
- Deliver high radiation dose in 3D volumes that conform to the
shape of the tumor and involved nodes
- Reduce dose administered to normal tissues
- Can be delivered in two ways :
A) Use of geometric field shaping analogues
B) Combination of field shaping with modulation of intensity
(IMRT)
127. CONFORMATIONAL RADIOTHERAPY
- Careful delineation of macroscopic / gross tumor volume
(GTV)
- Regions likely to harbor microscopic disease – clinically
target volume (CTV)
- Planning target volume :
: will exclude the normal tissue to be spared from radiations
: will generate the volume to compensate patient movement or
other inaccuracies
128. ▪ Hormesis is a dose response phenomenon in which small
doses of a toxin have the opposite effect of large doses
▪ E.g. exposing mice to small doses of radiation shortly before
exposing them to very high levels of radiation actually
decreases the likelihood of cancer
Hormesis
129. ▪ The initial low dose of radiation may activate certain repair
mechanisms in the body and these mechanisms are efficient enough
to not only neutralize the radiation effects but may even repair other
defects not caused by the radiation
▪ There is a lot of debate about hormesis, but the general opinion is that
this is not something that can be relied on when discussing the effects
of radiation exposure