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PRESENTATION ON ROLE OF 
NUCLEOTIDE 
BY-MUSHARRAF 
ALI 
BRANCH-BT-4YR
WHAT IS NUCLEOTIDE? 
• NUCLEOTIDE = NUCLEOSIDE + 
PHOSPHATE 
• NUCLEOSIDE = SUGAR + N.B. 
• sugar-pentose sugar either ribose sugar(in 
RNA) & deoxyribose sugar(in DNA) 
• N.B.=Nitrogenous Base may be 
Purine(Adenine & Guanine) & 
Pyrimidine( Cytosine, Thymine & Uracil)
MAINLY TWO MAJOR IMP. ROLE OF 
NUCLEOTIDES ARE- 
1- AFTER POLYMERISATION-it 
form either DNA OR RNA. 
2- ACT AS PRECURSOR FOR- 
• energy currency( like ATP ) 
• coenzyme(like NAD+,NADP+,FAD,Co-A) 
• secondary messenger( cAMP, cGMP) 
• regulatory molecule( like ppGpp)
1- AFTER POLYMERISATION-it 
form either DNA OR RNA 
• In case of cellular organism DNA act as a 
genetic material 
• In case of non-cellular organism( like 
viruses, viroid & virusoid)- either DNA or 
RNA as a genetic material
2- ACT AS PRECURSOR FOR-A- 
ENERGY CURRENCY-Like- 
ATP( Adenosine Tri 
Phosphate)
Energy of hydrolysis of phosphate bonds 
• hydrolysis of high-energy phosphate bond 
of ATP releases free energy of about 
-7.3 kcal/mol 
Energy used- 
-formation of new bond 
-change in conformation of molecules 
-signal amplification 
-transport molecules across membranes
B-Coenzyme(likeNAD+,FAD+,Co-A) 
• Nicotinamide adenine dinucleotide 
(NAD+) 
• structure-nicotinamide- 
ribose-p-p-adenosine 
IN CASE OF NADP+ 
- at 2`-OH of ribose of adenosine one 
phoshate group attached
• Flavin adenine dinucleotide (FAD)- 
Structure-riboflavin- 
p-p-adenosine 
• Co-A-Structure-beta- 
Mercaptoethylamine-Pantothenic 
acid-3`-Phosphoadenosine 
diphosphate(3`-P-ADP)
C- Secondary msngr( cAMP, cGMP) 
• like- cAMP(adenosine 3`,5`-cyclic monophosphate
D-Regulatory molecule( like ppGpp) 
like-ppGpp( 
5` diphospho Guanosine 3` di 
phosphate) 
• is produced in bacteria in response to a 
slowdown in protein synthesis during 
amino acid starvation 
• This nucleotide inhibits the synthesis of 
the rRNA and tRNA molecules
Structure of ppGpp
CONCLUSION & REFERENCES- 
• CONCLUSION- this information is only 
about the basic function/role of nucleotide. 
Here information is coded in such a 
manner to make concept clear. For detail 
information of Function of nucleotide 
kindly go for references. 
• REFERENCES- Lehninger Biochemistry 
book 
- cambell Biochem book

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Presentation on role of nucleotide

  • 1. PRESENTATION ON ROLE OF NUCLEOTIDE BY-MUSHARRAF ALI BRANCH-BT-4YR
  • 2. WHAT IS NUCLEOTIDE? • NUCLEOTIDE = NUCLEOSIDE + PHOSPHATE • NUCLEOSIDE = SUGAR + N.B. • sugar-pentose sugar either ribose sugar(in RNA) & deoxyribose sugar(in DNA) • N.B.=Nitrogenous Base may be Purine(Adenine & Guanine) & Pyrimidine( Cytosine, Thymine & Uracil)
  • 3. MAINLY TWO MAJOR IMP. ROLE OF NUCLEOTIDES ARE- 1- AFTER POLYMERISATION-it form either DNA OR RNA. 2- ACT AS PRECURSOR FOR- • energy currency( like ATP ) • coenzyme(like NAD+,NADP+,FAD,Co-A) • secondary messenger( cAMP, cGMP) • regulatory molecule( like ppGpp)
  • 4. 1- AFTER POLYMERISATION-it form either DNA OR RNA • In case of cellular organism DNA act as a genetic material • In case of non-cellular organism( like viruses, viroid & virusoid)- either DNA or RNA as a genetic material
  • 5. 2- ACT AS PRECURSOR FOR-A- ENERGY CURRENCY-Like- ATP( Adenosine Tri Phosphate)
  • 6. Energy of hydrolysis of phosphate bonds • hydrolysis of high-energy phosphate bond of ATP releases free energy of about -7.3 kcal/mol Energy used- -formation of new bond -change in conformation of molecules -signal amplification -transport molecules across membranes
  • 7. B-Coenzyme(likeNAD+,FAD+,Co-A) • Nicotinamide adenine dinucleotide (NAD+) • structure-nicotinamide- ribose-p-p-adenosine IN CASE OF NADP+ - at 2`-OH of ribose of adenosine one phoshate group attached
  • 8. • Flavin adenine dinucleotide (FAD)- Structure-riboflavin- p-p-adenosine • Co-A-Structure-beta- Mercaptoethylamine-Pantothenic acid-3`-Phosphoadenosine diphosphate(3`-P-ADP)
  • 9. C- Secondary msngr( cAMP, cGMP) • like- cAMP(adenosine 3`,5`-cyclic monophosphate
  • 10. D-Regulatory molecule( like ppGpp) like-ppGpp( 5` diphospho Guanosine 3` di phosphate) • is produced in bacteria in response to a slowdown in protein synthesis during amino acid starvation • This nucleotide inhibits the synthesis of the rRNA and tRNA molecules
  • 12. CONCLUSION & REFERENCES- • CONCLUSION- this information is only about the basic function/role of nucleotide. Here information is coded in such a manner to make concept clear. For detail information of Function of nucleotide kindly go for references. • REFERENCES- Lehninger Biochemistry book - cambell Biochem book