The document discusses a parallel session from the RRI Tools Final Conference in Brussels on November 21-22, 2016. The session focused on breaking down walls and changing structures between three universities in Calabria, Italy - the University of Calabria, the University Mediterranea of Reggio Calabria, and the University Magna Graecia of Catanzaro - and the Calabria Regional Government. Through a co-design process, the groups identified major issues, pursued strategic regional cooperation over competition, and signed the first long-term agreement for collaborative projects between the universities and region.
European Open Science Cloud & EOSCsecretariat.eu
Donatella Castelli, EOSCsecretariat
Presented at the 2nd SSHOC Consortium Meeting, Florence, 14-15 October 2019
Governance models for sustainable urban construction logistics: barriers for ...Walther Ploos van Amstel
Urban construction logistics has a large impact on cities. The topic of this paper is governance strategies for realising more sustainable urban construction logistics. Although not much research has been carried out in the field of governance of construction logistics, several authors have stressed the fragmented nature of the construction industry and the importance of collaboration in urban construction logistics. A literature review was conducted to identify sensitizing concepts on barriers in processes of collaboration. Based on these barriers we identified which drivers for collaborative governance are missing and which actions are necessary to improve the governance of construction logistics. The methods for data collection were semi-structured interviews and one focus group. The collaborative governance model is applied as a strategy to overcome the identified barriers in collaboration and governance. Key findings are both formal and informal barriers hinder the governance of construction logistics. On the basis of the collaborative governance model we identify four drivers that are necessary for collaborative governance to emerge.
Visual Analytics of Smart City Data for Sustainable Quality of Life of CitizensMikolaj Leszczuk
The improvement of Quality of Life (QoL) concerns every citizen. Fostering and applying research domains in advanced acquisition, modelling and visual analysis tools will support cities’ deciders in taking informed QoL improvement decisions on (partially) uncertain big data. We acknowledge a challenge in the scalability of complexity ranging from an expert’s view to a naive citizen’s understanding of (potentially unpopular) QoL decisions that we will tackle by new approaches to open big data network acquisition, multidimensional and multidisciplinary transformation of data, extraction of decision relevant data and its associated reliability, followed by appropriate visualisation aided by a data-aware digital assistant.
Presentation of the activities of the Multimedia Labs in the MEDINS project (immaterial cultural heritage) at the Malta technical meeting in November 2007
Sofia Knowledge City - Mickael Pero - 20171028Mickael Pero
COST representative, Mickael Pero Science Officer at COST provided an overview of ongoing knowledge networks working on smart city topics, as well as the current activity of Sofia communities in those knowledge networks. Presentation available here.
European Open Science Cloud & EOSCsecretariat.eu
Donatella Castelli, EOSCsecretariat
Presented at the 2nd SSHOC Consortium Meeting, Florence, 14-15 October 2019
Governance models for sustainable urban construction logistics: barriers for ...Walther Ploos van Amstel
Urban construction logistics has a large impact on cities. The topic of this paper is governance strategies for realising more sustainable urban construction logistics. Although not much research has been carried out in the field of governance of construction logistics, several authors have stressed the fragmented nature of the construction industry and the importance of collaboration in urban construction logistics. A literature review was conducted to identify sensitizing concepts on barriers in processes of collaboration. Based on these barriers we identified which drivers for collaborative governance are missing and which actions are necessary to improve the governance of construction logistics. The methods for data collection were semi-structured interviews and one focus group. The collaborative governance model is applied as a strategy to overcome the identified barriers in collaboration and governance. Key findings are both formal and informal barriers hinder the governance of construction logistics. On the basis of the collaborative governance model we identify four drivers that are necessary for collaborative governance to emerge.
Visual Analytics of Smart City Data for Sustainable Quality of Life of CitizensMikolaj Leszczuk
The improvement of Quality of Life (QoL) concerns every citizen. Fostering and applying research domains in advanced acquisition, modelling and visual analysis tools will support cities’ deciders in taking informed QoL improvement decisions on (partially) uncertain big data. We acknowledge a challenge in the scalability of complexity ranging from an expert’s view to a naive citizen’s understanding of (potentially unpopular) QoL decisions that we will tackle by new approaches to open big data network acquisition, multidimensional and multidisciplinary transformation of data, extraction of decision relevant data and its associated reliability, followed by appropriate visualisation aided by a data-aware digital assistant.
Presentation of the activities of the Multimedia Labs in the MEDINS project (immaterial cultural heritage) at the Malta technical meeting in November 2007
Sofia Knowledge City - Mickael Pero - 20171028Mickael Pero
COST representative, Mickael Pero Science Officer at COST provided an overview of ongoing knowledge networks working on smart city topics, as well as the current activity of Sofia communities in those knowledge networks. Presentation available here.
Los recursos administrativos son actuaciones de los particulares en los que se solicita de la Administración la revisión o revocación de una resolución administrativa o de un acto de trámite
La nuova era dei robots: grandi opportunità e rischiCoppa+Landini
Andrea Forni, Fondatore di Investirobot - Osservatorio sugli Investimenti in Robotica - e del Meetup Italiano di Robotica, è intervenuto all'Explore Talk on "Ro-bots" organizzato da Coppa+Landini il 1 marzo 2017 in Copernico Milano.
Ha condiviso con il pubblico insight sul mercato della robotica fisica e di processo, sulla crescita del mercato dei roboadvisor e delle auto a guida autonoma. Sono tutti ambiti che si prospettano come opportunità di investimento interessanti per i prossimi 10 anni.
Si sta prospettando uno scenario per cui, da un lato si stima che sono stati creati 10 milioni di posti di lavoro grazie alla robotica, dall'altro che in un futuro non molto lontano circa il 47% dei lavori umani verranno sostituiti dall'intelligenza artificiale.
Una fetta consistente del nuovo mercato è costituito dalle società che producono droni e software per droni, società di servizi collegati ai droni e società assicurative collegate al rischio volo.
We had another great webinar presented by Dave Hammarberg (Director of IT and Consulting Senior Manager) and Jim Shellenberger (Senior Manager) with McKonly & Asbury! Thank you to everyone that attended and received CPE credit.
We discussed what skimming is and went into a discussion of several examples and how to detect and prevent your organization from becoming a victim of skimming.
Check out our Upcoming Events page for news and updates on our future seminars and webinars.
For more information on this topic or to submit a question for Dave or Jim, use our contact page at www.macpas.com/contact.
www.macpas.com/webinar-recap-skimming-what-the-auditors-miss
RRITools - questions pratiques pour améliorer l'ouverture et la transparenceMalvina ARTHEAU
Dans le cadre de la définition et de la compilation de « bonnes » pratiques en matière de Recherche et d’Innovation Responsables (Workpackage n°1), l’institut Athena, VU Université d’Amsterdam a produit un ensemble de questions pour accompagner la mise en place de critères et/ou d’indicateurs d’auto-évaluation pour les structures qui souhaitent améliorer leurs pratiques de recherche et/ou d’innovation responsable.
Ces questions sont issues, à la fois d’un travail bibliographique, des résultats de consultations et de l’analyse de pratiques existantes réalisés dans le cadre du projet ainsi que de l’apport de partenaires et experts.
Elles sont regroupées pour répondre aux quatre groupes de critères de définition de la recherche et l’innovation responsables dans leur mise en œuvre :
> La diversité et l’inclusion
> L’ouverture et la transparence
> L’anticipation et la réflexivité
> La réactivité et l’adaptation au changement
L’ensemble du document explicitant les objectifs de ces questions et la méthode de leur élaboration est disponible sur le site RRITools : Livrable n°1.3 – en anglais
Auteurs: RRITools, Athena Institute, Malvina Artheau
CC BY NC SA
What´s that thing called RRI? By Jacqueline Broerse RRI Tools
What´s that thing called RRI?
Jacqueline Broerse
Director of the Athena Institute, VU University Amsterdam
RRI Tools Final Conference - Brussels, 21-22 November 2016
Opening Session
RRI Tools: main goals and outcomes
Los recursos administrativos son actuaciones de los particulares en los que se solicita de la Administración la revisión o revocación de una resolución administrativa o de un acto de trámite
La nuova era dei robots: grandi opportunità e rischiCoppa+Landini
Andrea Forni, Fondatore di Investirobot - Osservatorio sugli Investimenti in Robotica - e del Meetup Italiano di Robotica, è intervenuto all'Explore Talk on "Ro-bots" organizzato da Coppa+Landini il 1 marzo 2017 in Copernico Milano.
Ha condiviso con il pubblico insight sul mercato della robotica fisica e di processo, sulla crescita del mercato dei roboadvisor e delle auto a guida autonoma. Sono tutti ambiti che si prospettano come opportunità di investimento interessanti per i prossimi 10 anni.
Si sta prospettando uno scenario per cui, da un lato si stima che sono stati creati 10 milioni di posti di lavoro grazie alla robotica, dall'altro che in un futuro non molto lontano circa il 47% dei lavori umani verranno sostituiti dall'intelligenza artificiale.
Una fetta consistente del nuovo mercato è costituito dalle società che producono droni e software per droni, società di servizi collegati ai droni e società assicurative collegate al rischio volo.
We had another great webinar presented by Dave Hammarberg (Director of IT and Consulting Senior Manager) and Jim Shellenberger (Senior Manager) with McKonly & Asbury! Thank you to everyone that attended and received CPE credit.
We discussed what skimming is and went into a discussion of several examples and how to detect and prevent your organization from becoming a victim of skimming.
Check out our Upcoming Events page for news and updates on our future seminars and webinars.
For more information on this topic or to submit a question for Dave or Jim, use our contact page at www.macpas.com/contact.
www.macpas.com/webinar-recap-skimming-what-the-auditors-miss
RRITools - questions pratiques pour améliorer l'ouverture et la transparenceMalvina ARTHEAU
Dans le cadre de la définition et de la compilation de « bonnes » pratiques en matière de Recherche et d’Innovation Responsables (Workpackage n°1), l’institut Athena, VU Université d’Amsterdam a produit un ensemble de questions pour accompagner la mise en place de critères et/ou d’indicateurs d’auto-évaluation pour les structures qui souhaitent améliorer leurs pratiques de recherche et/ou d’innovation responsable.
Ces questions sont issues, à la fois d’un travail bibliographique, des résultats de consultations et de l’analyse de pratiques existantes réalisés dans le cadre du projet ainsi que de l’apport de partenaires et experts.
Elles sont regroupées pour répondre aux quatre groupes de critères de définition de la recherche et l’innovation responsables dans leur mise en œuvre :
> La diversité et l’inclusion
> L’ouverture et la transparence
> L’anticipation et la réflexivité
> La réactivité et l’adaptation au changement
L’ensemble du document explicitant les objectifs de ces questions et la méthode de leur élaboration est disponible sur le site RRITools : Livrable n°1.3 – en anglais
Auteurs: RRITools, Athena Institute, Malvina Artheau
CC BY NC SA
What´s that thing called RRI? By Jacqueline Broerse RRI Tools
What´s that thing called RRI?
Jacqueline Broerse
Director of the Athena Institute, VU University Amsterdam
RRI Tools Final Conference - Brussels, 21-22 November 2016
Opening Session
RRI Tools: main goals and outcomes
INCEPTION enriches the European identity through the understanding of how European Cultural Heritage continuously evolves over long periods of time. INCEPTION’s Inclusive approach introduces novel solutions of 3D digital modelling:
1 - forever: INCEPTION “Time Machine” that represents an innovative use of timescale for dynamic 3D reconstruction;
2 - for everybody: portable, user-friendly and cost-effective hardware and software instruments for 3D capturing, modelling and analysis;
3 - from everywhere: INCEPTION’s proposed standard procedures for data acquisition and open-standard format for Cultural Heritage Building Information Modelling.
For further information visit the website: www.inception-project.eu
"Clean Air and Urban Landscapes Hub (CAUL)"
Cathy Oke, Knowledge Broker, Clean Air and Urban Landscapes Hub presented an overview of her group as part of the SMART Seminar Series on 24 August 2016.
For more information, visit the event page at: http://smart.uow.edu.au/events/UOW219527.html
Introduction to the Reference Framework for Sustainable Cities, illustrated with local examples. Presentation by Pamela Mühlmann (ICLEI) & Koos van Dijken (Platform31), RFSC Conference 2013.
7th Session: Workshop II on a Roadmap to Future Government
In this session we proceed to presentations and discussion concerning the the development of the new roadmap for digital government. Two projects (Gov3.0 and Big Policy Canvas) will join forces in this exciting endeavor.
Organizers: Maria Wimmer, Professor, Koblentz University, Germany; Francesco Mureddu, Associate Directorr, Lisbon Council, Belgium; Juliane Schmeling Fraunhofer Institut FOKUS, Researcher, Germany; Shoumaya Ben Dhaou, Researcher, United Nations University, PT
This presentation is a brief description of our international project on Utility Services.
This project deals with Big Data, Open Data, R analytics.
Universities and Industry working together sharing knowledge and expertise.
Only by private funding by the moment.
Knowledge and technology transfer is our main goal.
For more information about the Informed Cities initiative visit http://informed-cities.iclei-europe.org or join us on Facebook at https://www.facebook.com/InformedCities
Project Initiation Routemap - Denise Bower, United KingdomOECD Governance
Presentation made by Denise Bower, University of Leeds / Major Projects Association, United Kingdom, at the Symposium on Governance of Infrastructure held at the OECD, Paris, on 29 February 2016
Similar to Presentation by Bernardo Rondelli and Solagne Chavel, SIRIS Academic (20)
Responsible research and innovation in a rapidly changing and increasingly te...RRI Tools
Responsible research and innovation in a rapidly changing and increasingly telecoupled world
Barron Joseph Orr
Professor and Geospatial Extension Specialist, University of Alicante & University of Arizona
RRI Tools Final Conference - Brussels, 21-22 November 2016
PLENARY SESSION: Facing the societal challenges of our time
Food security - RRI in Agricultural research for development. By Pascal KosuthRRI Tools
Food security - RRI in Agricultural research for development
Pascal Kosuth
Director of the Agropolis Foundation
RRI Tools Final Conference - Brussels, 21-22 November 2016
PLENARY SESSION: Facing the societal challenges of our time
How can Open Science and responsible medical research meet the challenge of i...RRI Tools
How can Open Science and responsible medical research meet the challenge of improving health?
Trish Groves
Editor-in-chief, BMJ Open and Honorary deputy editor, The British Medical Journal
RRI Tools Final Conference - Brussels, 21-22 November 2016
PLENARY SESSION: Facing the societal challenges of our time
Going Global: international perspectives on responsibility. By María VillavecesRRI Tools
Going Global: international perspectives on responsibility
María Villaveces
Chief executive officer, Colombian Association for the Advancement of Science Based Research and Social Responsibility in Higher Education.
RRI Tools Final Conference - Brussels, 21-22 November 2016
Going Global: international perspectives on responsibility. By Rajesh Tandon RRI Tools
Going Global: international perspectives on responsibility
Rajesh Tandon
President of Participatory Research in Asia and co-Chair of the UNESCO Chair in Community
RRI Tools Final Conference - Brussels, 21-22 November 2016
The RRI HUBS: In the name of responsible research and Innovation. By Rosalia ...RRI Tools
The RRI HUBS: In the name of responsible research and Innovation
Rosalia Vargas
President of Ciência Viva - Agência Nacional para a Cultura Científica e Tecnológica
RRI Tools Final Conference - Brussels, 21-22 November 2016
Opening Session
RRI Tools: main goals and outcomes
Delivering a pan-European Programme of Training & Advocacy. By Melanie SmallmanRRI Tools
Delivering a pan-European Programme of Training & Advocacy
Melanie Smallman
Department of Science and Technology Studies, University College London
RRI Tools Final Conference - Brussels, 21-22 November 2016
Opening Session
RRI Tools: main goals and outcomes
Challenging responsibility: How to make RRI work in a sustainable manner. By ...RRI Tools
Challenging responsibility: How to make RRI work in a sustainable manner
Ulrike Felt, Professor of Science and Technology Studies, Dean of the Faculty of Social Sciences, University of Vienna
RRI Tools Final Conference
Opening Lecture
Brussels, 21 November 2016
RRI Tools webinar - Become an RRI pioneer at your schoolRRI Tools
Webinar held by European Schoolnet on 19 July 2016
Video of the webinar: https://www.youtube.com/watch?v=XaYAcQ81C0s
The integration of Responsible Research and Innovation principles in teaching and learning activities supports multidisciplinarity and stronger students' engagement as well as their acquisition of critical thinking and collaborative learning skills.
It also prepares them to make informed and evidence-based choices about society’s future and help them to better understand existing careers paths, entrepreneurship and innovation processes, and complexities of the professional world.
By Lars Klüver, Coordinator of Engage2020, CIMULACT and HBP Citizen Consultations and Director of the Danish Board of Technology Foundation
Presentation at the second RRI Tools Train the Trainers, July 2016
The European project RRI Tools aims at building a Responsible Research & Innovation Toolkit, with the concerned stakeholders, to make RRI happen in real life!
More information about RRI Tools: http://www.rri-tools.eu/
Latest news: http://blog.rri-tools.eu/
Twitter: https://twitter.com/RRITools
Facebook: https://www.facebook.com/RRI.Tools.Project?fref=ts
LinkedIn: https://www.linkedin.com/grp/home?gid=8111498
YouTube: https://www.youtube.com/channel/UCiXjh5T5uu2ka7NpbQkzFrA
Flickr: https://www.flickr.com/photos/130674936@N05/
RRI Toolkit for EU National Contact PointsRRI Tools
Overview of what is RRI and how the RRI Toolkit -developed by the RRI Tools project- can help to implement it accross Europe, especially through the National Contact Points (NCPs).
This presentation was given by Eva Zuazua and Daniel Garcia (La Caixa Foundation) in the NCPs network meeting (SiS.net) held in Madrid (Spain) on December 2nd, 2015.
Lightning talk (5') explaining the commonalities between citizen science and the Responsible Research and Innovation (RRI) approach. The talk was given by Daniel Garcia (La Caixa Foundation), from the RRI Tools project (www.rri-tools.eu) at the 2015 European Citizen Science Assembly in Barcelona.
Sparks Projects is a new EU-funded project, which through exhibitions all around Europe will spread the word about RRI in a creative, arty and innovative way.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
2. |
RRI Tools Final Conference, 21-22 November 2016, Brussels
which structure
• 3 Universities
• Univ. of Calabria
• Univ. Mediterranea of Reggio
Calabria,
• Univ. Magna Graecia of Catanzaro
• Calabria Regional Government
| Breaking Walls, changing structures
3. |
RRI Tools Final Conference, 21-22 November 2016, Brussels
• co-design process to identity major
issues and challenges
• strategic focus across the whole
regional system
• cooperation vs. competition among
the different stakeholders
| Breaking Walls, changing structures
change and impact
4. |
RRI Tools Final Conference, 21-22 November 2016, Brussels
• 1st framework agreement for a long
term project region-universities
• new methodology for designing
projects and monitoring
implementation
• increasing the awareness and
accountability in the use of public funds
for regional development
| Breaking Walls, changing structures
results