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This document discusses Escherichia coli (E. coli), a type of bacteria commonly found in the intestines of humans and animals. It can be part of normal intestinal flora but some strains cause illness. The document describes E. coli's characteristics such as being a gram-negative bacillus and facultative anaerobe. It also discusses different types of pathogenic E. coli and their virulence factors. The second half of the document focuses on methods for detecting and enumerating beta-glucuronidase-positive E. coli using a tryptone-bile-glucuronic medium at 44°C on a petri dish. It provides the procedure, incubation times, and formulas for calculating colony
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This document provides information on Enterobacteriaceae and pathogenic E. coli:
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The document describes several biochemical tests used to identify bacteria, including the catalase test, coagulase test, DNase test, oxidase test, indole test, urease test, citrate test, and triple sugar iron (TSI) test. It provides the purpose, principle, method, and results for each test. It also discusses how to specifically identify Staphylococcus aureus and Streptococcus pyogenes through microscopic examination, culture characteristics, and biochemical properties.
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This document discusses calf diarrhea, a major cause of economic loss in the cattle industry. It notes that infectious agents like viruses (rotavirus, coronavirus), bacteria (E. coli, Salmonella species, Clostridium perfringens), and protozoa (Eimeria, Cryptosporidium) are leading causes of neonatal calf diarrhea. Clinical signs can include anorexia, fever, depression, watery to bloody diarrhea. Diagnosis involves isolating and identifying the causal bacteria or virus through culturing fecal samples and performing biochemical tests. Management practices like ensuring adequate colostrum intake and hygiene can help prevent calf diarrhea.
Staphylococcus aureus bacteria ppt
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2. It covers the major clinical manifestations of enteric infections like diarrhea, vomiting, and abdominal pain.
3. Laboratory diagnosis of enteric bacteria involves using differential and selective media like MacConkey agar and Eosin methylene blue agar to differentiate lactose fermenters from non-fermenters. Tests like triple sugar iron agar also help identify bacteria.
Intestinal coli infection PPT
Intestinal Coli Infection, also known as Escherichiosis, is caused by the Escherichia coli bacterium entering the body through the mouth and traveling to the small bowel. In the small bowel, the pathogens reproduce and produce enterotoxins and endotoxins. This leads to inflammatory changes and damage to the small intestine epithelium. The endotoxins cause toxic symptoms while the bacteria can spread to other parts of the body. Antibiotics are used to treat the infection but the choice depends on diagnosing the specific strain. Treatment also involves dietary changes in the acute phase.
E. coli
I am Tariq Bin Aziz, From Southeast University, Bangladesh. I made this presentation on E.coli. I think you will be benefited by my presentation. Thanks All.
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Medi 4303-i-bacterial infections of the gastrointestinal tract-i--diarrhoea
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Guest Speaker Segment with Hannah Barrington: Dive into the world of dynamic real estate marketing with Hannah, the Marketing Manager at Workspace Group. Hear firsthand how their team generates engaging descriptions for thousands of office units by integrating diverse data sources—from PDF floorplans to web pages—using FME transformers, like OpenAIVisionConnector and AnthropicVisionConnector. This use case will show you how GenAI can streamline content creation for marketing across the board.
Ollama Use Case: Learn how Scenario Specialist Dmitri Bagh has utilized Ollama within FME to input data, create custom models, and enhance security protocols. This segment will include demos to illustrate the full capabilities of FME in AI-driven processes.
Custom AI Models: Discover how to leverage FME to build personalized AI models using your data. Whether it’s populating a model with local data for added security or integrating public AI tools, find out how FME facilitates a versatile and secure approach to AI.
We’ll wrap up with a live Q&A session where you can engage with our experts on your specific use cases, and learn more about optimizing your data workflows with AI.
This webinar is ideal for professionals seeking to harness the power of AI within their data management systems while ensuring high levels of customization and security. Whether you're a novice or an expert, gain actionable insights and strategies to elevate your data processes. Join us to see how FME and AI can revolutionize how you work with data!
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みなさんこんにちはこれ何文字まで入るの？40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの？えこ...
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POWERPOINT templet - Copy.pptx
- 2. 2 • The most famous family member of Enterobacteriaceae. • It known to be a part of normal intestinal flora but can also be the cause of intestinal and extraintestinal illness in humans. • Some versions of E. coli make you sick by making a toxins, this toxins can damage the lining of your intestine One especially bad strain, O157:H7 Escherichia coli
- 3. 3 Normal intestinal flora •Gram-negative bacillus Flexible flagella Escherichia coli
- 4. 4 Facultative anaerobes •Oxidase Negative Catalase Positive Escherichia coli
- 5. People usually get infected by : 5 Escherichia coli
- 6. 6 Escherichia coli E. coli Types : •Enteropathogenic E. coli •(EPEC) •Enterotoxigenic E. coli •(ETEC) •Enteroinvasive E. coli •(EIEC) •Shiga-Toxin Producing E. coli (STEC)
- 7. Virulence factors of E. coli : 7 Escherichia coli • Endotoxins • Exotoxins • Phili • Capsule
- 8. 8 Escherichia coli EMB Blue-black colonies with a green metallic sheen MacConkey Agar pink to dark pink colonies
- 9. Horizontal method for the detection and enumeration of ß- glucuronidase-positive Escherichia coli Colony-count technique at 44 °C using 5-bromo-4chloro-3-indolyl ß-glucuronide 9 ISO 16649-2 Escherichia coli
- 10. 10 • Scope o Applicable to products intended for human and the feeding of animals at 44 °C using solid medium containing a chromogenic ingredient for detection of the enzyme ß-glucuronidase. • Terms and definitions ß-glucuronidase-positive Escherichia coli : o Bacteria which form typical blue colony on TBX at 44 °C Escherichia coli
- 11. 11 • Culture medium Tryptone-bile-glucuronic medium (TPX) Enzymatic Digest of Casein Provides amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Bile Salts selective agent inhibiting most Gram-positive bacteria. X-Glucuronide the chromogenic substrate cleaved by the enzyme β-glucuronidase of E. coli. Agar is the solidifying agent. Agar The solidifying agent. Escherichia coli
- 12. 12 • Culture medium Tryptone-bile-glucuronic medium (TPX) o Chromogenic selective medium o Adjust the pH after sterilization at 7.2 ± 0,2 at 25 °C o Sterilize at 121 °C for 15 min in an autoclave o Cool the medium in the water bath at 44 °C to 47 °C . Escherichia coli
- 13. 13 • Procedure o Homogenize the test representative sample with Saline Peptone Water (9:1) o Add 1ml of the appropriate dilution to petri dish (Inoculate two plates per dilution) o Repeat with further dilution, using a new sterile pipette o Pour 15ml of the TBX medium into each petri dish Escherichia coli
- 14. 14 Incubation : o Incubate at 44 °C for 18h to 24h o The total incubation time shall not longer than 24h o Invert the inoculated dished The time elapses between the distribution of the inoculum in the dishes and pouring the medium shall not exceed 15min If the presence of stressed cells is suspected, incubate for initial period of 4h at 37 C and then raise the incubation temp. to 44 for 18h to 24h. Escherichia coli
- 15. 15 Blue to blue-green colonies • Result Escherichia coli
- 16. 16 • Counting the colony-forming unit Escherichia coli o Count the typical CFU in each dish containing less than 150 typical CFU and less than 300 total (typical and non-typical) CFU o The CFU at least on one dish containing as a minimum 15 blue CFU o 𝑁 = 𝞢𝑎 𝑉 𝑛1+0.1𝑛2 𝑑
- 17. 17 Special cases : o When the number of the blue colonies is higher than 150 for the two dishes from the first dilution 𝑑1 , and the number of the blue colonies is less than 15 for the subsequent dilution 𝑑2 : - If the number of blue CFU for the two dishes from 𝑑1 is within the range 150 to 167 use the calculation method : 𝑁 = 𝞢𝑎 𝑉 𝑛1+0.1𝑛2 𝑑 - If the number of blue CFU for the two dishes from 𝑑1 is higher than 167, only take into account the low number count, of 𝑑2 : 𝑁 = 𝞢𝑐 𝑉 . 𝑛 . 𝑑
- 18. 18