planning of semen bank.pptx
•Download as PPTX, PDF•
0 likes•21 views
The document outlines the key components and requirements for constructing an effective semen bank, including separate areas for semen collection, evaluation, storage, animal housing and exercise, quarantine, and staff housing. It emphasizes the need for clean and restricted access laboratories, proper housing and care of bulls, and equipment for semen collection, processing, evaluation and storage.
Report
Share
Report
Share
Recommended
Housing and Caging.pptx
The document discusses guidelines for housing laboratory animals including the following:
1. The location of the animal house should be separated from noise, pollutants, and wild animals. Rooms are needed for isolation, quarantine, cage washing, storage, diet preparation, and offices.
2. Cages should allow for easy observation and cleaning of animals while minimizing disease transmission. Common cage types include solid floor, wire grid floor, and metabolic cages.
3. Proper bedding, feeding devices, water sources, and diets must be provided depending on the animal species. Wastes must be collected and disposed of carefully along with regular cleaning and vermin control in the animal house.
Meagan Roberts "Pickin The Right Chicken" SSAWG2018
This document provides information on housing, processing, and marketing poultry cooperatively. It discusses artificial brooding methods for chicks, including box brooders, area brooders, and hover brooders. Recommendations are given for space, heat, bedding, ventilation, and feeding requirements for chicks. Housing guidelines are outlined for adult chickens, including proper space, roosts, ranges, yards, and free range options. The document also covers sanitary processing practices and required equipment for killing, scalding, plucking, evisceration, and chilling birds. Finally, it discusses models for cooperative chick, meat, and egg sales through coordinated production and marketing.
Ubaid afzal (4)
A tissue culture lab requires specific equipment and facilities to successfully generate new plants through culturing cells and tissues. The lab needs separate rooms for transferring tissues, culturing under controlled conditions, refrigerating media and reagents, washing equipment, and transplanting new plants. Essential equipment includes glassware for sterilizing media, biosafety cabinets for sterile work, incubators for maintaining ideal growth conditions, centrifuges for media preparation, and microscopes for observations. Precise environmental controls and aseptic techniques are necessary throughout the process to produce healthy plants in the laboratory setting.
Plant Tissue Culture- Introduction & History
Plant tissue culture is a trending and a promising area due to its applications. hence knowledge of its basics and history would be helpful for the pioneers. ans this presentation also discusses about the structure and establishment of a commercial tissue culture laboratory.
Growel' Commercial Layers Management Guide
The golden rule of management is to have one age and one breed per site to ensure the "all-in, all-out" principle is followed at all times.
The choice of the site for the farm, including the layout of the houses, must prioritise the elimination of all possible sources of contamination. Biosecurity protection is reinforced by hygiene controls.
A changing room should be made available at the entrance of the site. It must be used by everybody entering the farm (incorporating both a shower and a change of clothes).
When the old flock is removed and before the arrival of the new flock, all houses and equipment must be thoroughly cleaned and disinfected according to strict procedures and protocols. This should be followed by a rest period of at least 10 days.
Between each flock, cleaning and disinfection of the houses, their annexes,surroundings and access ways are essential to ensure the optimal health conditions required for the incoming flock to maximise its profitability.
Dr. Yogesh Sumthane Assistant Professor College of Forestry Banda University ...
⦿ This document discusses key considerations for establishing a successful tissue culture laboratory. It addresses the importance of strategic planning, proper location, and laboratory design to maintain aseptic conditions. A standard tissue culture lab should have areas for media preparation, sterilization, glassware washing, storage, aseptic transfer, and a primary growth room with environmental controls. Careful attention to traffic flow, lighting, temperature, humidity and other factors can optimize the lab's function. Networking, quality control, and market research are also important for a profitable tissue culture business.
final poultry (1) [Autosaved].pptx
Poultry Housing System FOR BETTER FARMING OF POULTRY AND APPLICATION OF UV LIGHT AND ENZYME TECHNOLOGY FOR POULTRY PRODUCTION
control shed rearing of poultry.pptx
This document discusses different poultry housing systems and management practices. It describes free range, semi-intensive, and intensive systems where birds are totally confined to houses. Intensive systems include cage rearing using different types of cages. The document also outlines the equipment, growth phases from brooding to adult layers, farm management practices like litter management, and benefits of cage rearing like high stock density and clean egg production. It concludes by noting future trends toward more organic and free range farming systems.
Recommended
Housing and Caging.pptx
The document discusses guidelines for housing laboratory animals including the following:
1. The location of the animal house should be separated from noise, pollutants, and wild animals. Rooms are needed for isolation, quarantine, cage washing, storage, diet preparation, and offices.
2. Cages should allow for easy observation and cleaning of animals while minimizing disease transmission. Common cage types include solid floor, wire grid floor, and metabolic cages.
3. Proper bedding, feeding devices, water sources, and diets must be provided depending on the animal species. Wastes must be collected and disposed of carefully along with regular cleaning and vermin control in the animal house.
Meagan Roberts "Pickin The Right Chicken" SSAWG2018
This document provides information on housing, processing, and marketing poultry cooperatively. It discusses artificial brooding methods for chicks, including box brooders, area brooders, and hover brooders. Recommendations are given for space, heat, bedding, ventilation, and feeding requirements for chicks. Housing guidelines are outlined for adult chickens, including proper space, roosts, ranges, yards, and free range options. The document also covers sanitary processing practices and required equipment for killing, scalding, plucking, evisceration, and chilling birds. Finally, it discusses models for cooperative chick, meat, and egg sales through coordinated production and marketing.
Ubaid afzal (4)
A tissue culture lab requires specific equipment and facilities to successfully generate new plants through culturing cells and tissues. The lab needs separate rooms for transferring tissues, culturing under controlled conditions, refrigerating media and reagents, washing equipment, and transplanting new plants. Essential equipment includes glassware for sterilizing media, biosafety cabinets for sterile work, incubators for maintaining ideal growth conditions, centrifuges for media preparation, and microscopes for observations. Precise environmental controls and aseptic techniques are necessary throughout the process to produce healthy plants in the laboratory setting.
Plant Tissue Culture- Introduction & History
Plant tissue culture is a trending and a promising area due to its applications. hence knowledge of its basics and history would be helpful for the pioneers. ans this presentation also discusses about the structure and establishment of a commercial tissue culture laboratory.
Growel' Commercial Layers Management Guide
The golden rule of management is to have one age and one breed per site to ensure the "all-in, all-out" principle is followed at all times.
The choice of the site for the farm, including the layout of the houses, must prioritise the elimination of all possible sources of contamination. Biosecurity protection is reinforced by hygiene controls.
A changing room should be made available at the entrance of the site. It must be used by everybody entering the farm (incorporating both a shower and a change of clothes).
When the old flock is removed and before the arrival of the new flock, all houses and equipment must be thoroughly cleaned and disinfected according to strict procedures and protocols. This should be followed by a rest period of at least 10 days.
Between each flock, cleaning and disinfection of the houses, their annexes,surroundings and access ways are essential to ensure the optimal health conditions required for the incoming flock to maximise its profitability.
Dr. Yogesh Sumthane Assistant Professor College of Forestry Banda University ...
⦿ This document discusses key considerations for establishing a successful tissue culture laboratory. It addresses the importance of strategic planning, proper location, and laboratory design to maintain aseptic conditions. A standard tissue culture lab should have areas for media preparation, sterilization, glassware washing, storage, aseptic transfer, and a primary growth room with environmental controls. Careful attention to traffic flow, lighting, temperature, humidity and other factors can optimize the lab's function. Networking, quality control, and market research are also important for a profitable tissue culture business.
final poultry (1) [Autosaved].pptx
Poultry Housing System FOR BETTER FARMING OF POULTRY AND APPLICATION OF UV LIGHT AND ENZYME TECHNOLOGY FOR POULTRY PRODUCTION
control shed rearing of poultry.pptx
This document discusses different poultry housing systems and management practices. It describes free range, semi-intensive, and intensive systems where birds are totally confined to houses. Intensive systems include cage rearing using different types of cages. The document also outlines the equipment, growth phases from brooding to adult layers, farm management practices like litter management, and benefits of cage rearing like high stock density and clean egg production. It concludes by noting future trends toward more organic and free range farming systems.
Central sterile supply department(cssd)
The Central Sterile Supply Department (CSSD) is responsible for receiving, processing, sterilizing, storing and distributing sterile supplies and equipment for hospitals. Key functions of the CSSD include cleaning, packaging, sterilizing items using autoclaves or ethylene oxide, storing sterile supplies, and issuing items to different hospital units. CSSDs aim to provide safe sterile supplies efficiently while standardizing equipment and assisting in infection control.
design and operations of Hatchery.pptx
Hatchery design
Hatchery should be constructed in such a manner that the hatching eggs may be taken in one end and the chicks removed at the other.
In other words, eggs and chicks should flow through the hatchery from one room to the one next needed in the hatching process.
There should not be no backtracking.
Size of hatchery depends on no.of hatchings per week and no.of chicks hatched at one time determines the capacity of the machines required no.of machines and working space
Hatchery construction
Width: The width of the setter and hatcher rooms is to be determined by the type of the incubator used. Find the depth of the incubators; then allow space for the working aisles, behind the machines and the walls.
Height of the ceiling: The height of the ceiling should be at least 16 ft.
Ceiling material: Most hatchery rooms have a high humidity, and during cold weather condensation of moisture on the ceilings is common. Hence, the ceiling material is to be waterproof.
Walls: Fireproof material should be used in constructing the walls of the hatchery building as possible. Because the interior of the hatchery building is continually being washed and disinfected, the inside walls should be covered with a glazed hard nonabsorbent finish. This finish also prevents the growth of molds common to walls that are porous and absorbent.
Doors: wide enough for easy movement of trolleys, chick boxes etc. The door openings should be 8 ft high and at least 4 ft wide, and doors double-swinging.
Floor: All floors must be concrete, preferably with imbedded steel to prevent cracking. The concrete must be given a glazed finish. Slope of the floor should never be greater than 0.5 inch in 10 feet.
Sewers: Since very large amount of water is utilized for hatchery operations and the possibilities for settling of broken eggshells in the sewer lines, it is necessary to have larger sewers in hatchery than those used in most industrial buildings.
Waterlines
Docks
Electricity lines
Egg receiving room – 4sqft per 1000 eggs ( should not be < 600 Sq ft)
Egg storage room –
Fumigation room
Prewarming room – 22-23ºC & 45% RH
Setter room – 25-26ºC & 45-55% RH
Candling space
Hatcher room – 25-26ºC & 60-70% RH
Chick handling room – 23-29 ºC & 50-60% RH
Growel Layer Farming Management Guide
The golden rule of management is to have one age and one breed per site to ensure the "all-in, all-out" principle is followed at all times.
The choice of the site for the farm, including the layout of the houses, must prioritise the elimination of all possible sources of contamination. Biosecurity protection is reinforced by hygiene controls.
Storage and_shipment_off_semen__1_.pdf
- Short term storage of semen involves storing semen at ambient or refrigerated temperatures for a few days using extenders. Long term storage involves cryopreservation of semen in liquid nitrogen.
- Proper handling and storage of frozen semen involves keeping straws submerged in liquid nitrogen, regularly topping off liquid nitrogen, and minimizing temperature fluctuations during transport.
- Transport of liquid semen involves using insulated containers with ice to keep temperatures below 10°C, while frozen semen requires special cryogenic containers and keeping the semen in liquid nitrogen during transport.
- Proper maintenance of liquid nitrogen containers involves regularly checking liquid nitrogen levels, minimizing openings to reduce evaporation, and avoiding thermal and physical damage to
microbiology_sample_collection.pptx
This document provides guidance on specimen management for nursing students. It discusses the importance of proper specimen collection, handling, transportation and storage to ensure quality laboratory test results. Key points include:
- Specimen management is important to reduce laboratory errors and involves proper instructions, collection, handling, transportation and storage.
- Universal precautions should be followed when collecting all specimens to prevent exposure to biohazards. Samples must be properly labeled and packaged for transport.
- The type of specimen and collection method depends on the infection or test being performed. Examples provided include blood, urine, stool and sputum collection procedures.
- Transport media is used to maintain specimen viability during transit to the laboratory and varies based on specimen type. Strict
To study plant tissue culture laboratory design and set up
This document provides guidelines for setting up a plant tissue culture laboratory, including necessary equipment and design considerations. It discusses key areas of the lab such as the culture room, media preparation area, and glassware washing area. Maintaining aseptic conditions is a primary focus of the design. Proper airflow, traffic flow, and separation of clean and dirty areas are emphasized. Necessary equipment includes a laminar hood, autoclave, incubator shaker, and supplies for tissue culture work. Adherence to safety protocols is also covered.
yasir labs designing
The document discusses the requirements and considerations for building and designing various sections of a clinical diagnostic laboratory. It covers architectural and engineering factors like lab module sizes, flexibility for expansion, and placement of utilities. It also describes the specific equipment, instruments, and facilities needed in different diagnostic areas like microbiology, parasitology, pathology, hematology, ELISA testing, PCR, and bacteriology sections. Proper ventilation, security, waste disposal, and health and safety measures are important across all laboratory areas.
Peculiarities of Abattoir and Meat Industry
An abattoir, or slaughterhouse, is a facility that processes animals for food products. Approximately 45-50% of the animal can be used for food, while 15% is waste and 40-45% becomes byproducts. Abattoirs must follow religious practices for slaughtering animals. They come in modern, semi-modern and traditional varieties. Proper site selection, infrastructure, hygiene practices and layout are important for efficient and sanitary operations. Key areas of an abattoir include lairage, slaughtering, processing, inspection, packaging and storage.
Isolation of algae
This document discusses methods for collecting and isolating microalgae samples. It describes collecting algae from water sources using nets or deep water samplers, and from soils using basters or scoops. Five isolation techniques are outlined: 1) micromanipulation using pipettes under a microscope, 2) centrifugal washing to remove bacteria, 3) spraying algae onto agar plates, 4) streak plating using loops, and 5) serial dilution plating in growth media. The document concludes that isolating local algae species can support environmental and industrial uses like biofuel production.
To develop a project for establishment of commercial tissue culture laboratory
The document provides guidelines for establishing a commercial tissue culture laboratory, including:
- The laboratory should have separate rooms for media preparation, glassware washing, sterilization, aseptic transfer, and primary culture growth to maintain cleanliness and prevent contamination.
- Facilities are needed for washing and sterilizing glassware, preparing and storing media, conducting aseptic procedures, and maintaining cultures under controlled conditions.
- Proper location, ventilation, lighting, temperature and humidity control are important considerations for laboratory design.
INSTRUMENTS USED IN MICROBIOLOGY LAB WITH PRINCIPLE AND.ppt
The document discusses various instruments used in microbiology labs, including their principles of operation and common uses. It describes analytical balances, autoclaves, Bunsen burners, centrifuges, colony counters, deep freezers, homogenizers, hot plates, hot air ovens, incubators, laminar flow hoods, magnetic stirrers, microscopes, pH meters, spectrophotometers, vortex mixers, water baths, water distillers, wire loops, Bijoh bottles, glassware, and Durham tubes. It also covers proper collection and transport of clinical specimens for microbiological analysis.
Cell culture
Equipment essential for animal cell culture includes an incubator for maintaining optimal temperature and CO2 levels, a sterile work area like a laminar flow hood, and storage equipment like refrigerators and freezers. Additional beneficial equipment includes microscopes, cell counters, centrifuges, and sterilization tools. Proper cell culture requires maintaining sterile conditions at all times using these various pieces of equipment.
Chicken broiler.pptx
This document provides information on chicken broiler and egg production in the Philippines. It discusses selecting chicken breeds, housing requirements, common diseases, and feeding management. The key points covered are guidelines for selecting foundation stocks, housing specifications based on stock type and production capacity, symptoms and prevention of common poultry diseases like Newcastle disease and fowl pox, and feeding recommendations for broiler and layer chickens at different growth stages.
Seed storage
This document discusses principles and methods for storing seeds to preserve their viability and quality until planting. It outlines the various stages and types of seed storage, as well as factors that affect seed longevity in storage like moisture content, temperature, and pests. Proper storage requires drying seeds to a safe moisture level before placing them in a cool, dry warehouse protected from fluctuations and pests. Cryopreservation in liquid nitrogen at -196°C can also preserve seeds indefinitely by halting biological processes.
Cpcsea guidelines
This document summarizes the CPCSEA (Committee for the Purpose of Control and Supervision of Experiments on Animals) guidelines for animal experimentation. It discusses the goals of humane animal care and avoiding unnecessary pain. It provides recommendations for housing, veterinary care, disease control, and husbandry practices. These include maintaining hygienic facilities, providing social environments, and disposing of waste properly. The document also outlines functions of the CPCSEA like registering facilities, approving protocols, and enforcing compliance. Standard operating procedures, transportation, anesthesia, and euthanasia methods are also addressed to ensure ethical treatment of laboratory animals.
Sample collection, Storage III.ppt
This document provides guidelines for sample collection, storage, transportation and labelling for laboratory testing. Key points include:
- Samples should be properly labelled with animal ID, date, owner details and tests requested.
- Transportation conditions like temperature and containers used must maintain sample integrity.
- Storage conditions vary by sample type but generally include refrigeration, freezing or using transport media.
- Guidelines are provided for collecting different sample types from live/dead animals including blood, serum, tissues and ensuring adequate documentation.
Equipments
Animal cell culture involves removing cells from an animal and growing them in an artificial conductive medium. It is used to produce therapeutic proteins and research diseases. Key equipment for animal cell culture includes biosafety cabinets for sterility, CO2 incubators to maintain temperature and pH, centrifuges to prepare cells, refrigerators/freezers for storage, and microscopes for analysis. Proper equipment is necessary to successfully culture cells outside their natural environment.
Housing management for better productivity.pptx
This document discusses housing management for livestock to improve productivity. It describes two main types of housing systems - loose housing and conventional barns. Loose housing involves keeping animals loose in an open paddock with a common shelter, while conventional barns confine animals together on a platform and secure them with stanchions for individual care and feeding. The document provides advantages and disadvantages of each system and discusses considerations for orientation, space requirements, roofing materials, and sanitation of livestock houses.
P (6) Autoclave and ovens.pdf
The document discusses two common laboratory sterilization instruments: autoclaves and dry ovens. Autoclaves use high-pressure steam to sterilize materials, while dry ovens use dry heat. There are different types of autoclaves including gravity displacement and pre-vacuum models. Sterilization cycles in autoclaves involve purging air, exposing items to high temperature/pressure, and exhausting steam. Dry ovens operate at temperatures over 180°C to kill bacterial spores using hot air, but are not suitable for heat-sensitive items. Proper use, maintenance and packaging are important for effective sterilization with both instruments.
M 1 sterlization
The document discusses various methods of sterilization including physical and chemical methods. Physical sterilization methods include heat (dry and moist), radiation, and filtration. Heat-based methods involve the use of dry heat through processes like flaming or hot air ovens, and moist heat through autoclaving, boiling, or tyndallization. Radiation methods include the use of ionizing radiation like gamma rays and non-ionizing radiation like UV light. Filtration uses filters of varying pore sizes to remove microorganisms from solutions. Chemical sterilization employs chemicals like alcohols, phenols, and oxidizing agents. The document outlines the factors influencing each sterilization method and their appropriate applications.
Handling of LN2.pptx
This document discusses the handling of cryogenic containers containing frozen semen for artificial insemination. It outlines the necessary equipment, including liquid nitrogen containers, dip sticks, artificial insemination guns, plastic sheaths, and forceps. It describes the process of thawing frozen semen straws and loading the straw into an AI gun with a plastic sheath for insemination. Safety precautions are provided for transporting and working with cryogenic containers to safely preserve frozen biological materials like semen.
breeding soundness exam in bulls.pptx
Breeding soundness evaluation (BSE) assesses a bull's reliability and capability for breeding through physical examination and evaluation of semen quality, libido, and freedom from disease. The evaluation has several components: physical exam; examination of external genitalia and reproductive organs; semen evaluation for motility, morphology, and infectious agents; and tests of mating ability and libido. Bulls are classified as satisfactory, deferred, or unsatisfactory breeders based on their scores on factors like scrotal circumference, semen motility, and morphology. Regular BSE can increase pregnancy rates by eliminating less fertile bulls.
More Related Content
Similar to planning of semen bank.pptx
Central sterile supply department(cssd)
The Central Sterile Supply Department (CSSD) is responsible for receiving, processing, sterilizing, storing and distributing sterile supplies and equipment for hospitals. Key functions of the CSSD include cleaning, packaging, sterilizing items using autoclaves or ethylene oxide, storing sterile supplies, and issuing items to different hospital units. CSSDs aim to provide safe sterile supplies efficiently while standardizing equipment and assisting in infection control.
design and operations of Hatchery.pptx
Hatchery design
Hatchery should be constructed in such a manner that the hatching eggs may be taken in one end and the chicks removed at the other.
In other words, eggs and chicks should flow through the hatchery from one room to the one next needed in the hatching process.
There should not be no backtracking.
Size of hatchery depends on no.of hatchings per week and no.of chicks hatched at one time determines the capacity of the machines required no.of machines and working space
Hatchery construction
Width: The width of the setter and hatcher rooms is to be determined by the type of the incubator used. Find the depth of the incubators; then allow space for the working aisles, behind the machines and the walls.
Height of the ceiling: The height of the ceiling should be at least 16 ft.
Ceiling material: Most hatchery rooms have a high humidity, and during cold weather condensation of moisture on the ceilings is common. Hence, the ceiling material is to be waterproof.
Walls: Fireproof material should be used in constructing the walls of the hatchery building as possible. Because the interior of the hatchery building is continually being washed and disinfected, the inside walls should be covered with a glazed hard nonabsorbent finish. This finish also prevents the growth of molds common to walls that are porous and absorbent.
Doors: wide enough for easy movement of trolleys, chick boxes etc. The door openings should be 8 ft high and at least 4 ft wide, and doors double-swinging.
Floor: All floors must be concrete, preferably with imbedded steel to prevent cracking. The concrete must be given a glazed finish. Slope of the floor should never be greater than 0.5 inch in 10 feet.
Sewers: Since very large amount of water is utilized for hatchery operations and the possibilities for settling of broken eggshells in the sewer lines, it is necessary to have larger sewers in hatchery than those used in most industrial buildings.
Waterlines
Docks
Electricity lines
Egg receiving room – 4sqft per 1000 eggs ( should not be < 600 Sq ft)
Egg storage room –
Fumigation room
Prewarming room – 22-23ºC & 45% RH
Setter room – 25-26ºC & 45-55% RH
Candling space
Hatcher room – 25-26ºC & 60-70% RH
Chick handling room – 23-29 ºC & 50-60% RH
Growel Layer Farming Management Guide
The golden rule of management is to have one age and one breed per site to ensure the "all-in, all-out" principle is followed at all times.
The choice of the site for the farm, including the layout of the houses, must prioritise the elimination of all possible sources of contamination. Biosecurity protection is reinforced by hygiene controls.
Storage and_shipment_off_semen__1_.pdf
- Short term storage of semen involves storing semen at ambient or refrigerated temperatures for a few days using extenders. Long term storage involves cryopreservation of semen in liquid nitrogen.
- Proper handling and storage of frozen semen involves keeping straws submerged in liquid nitrogen, regularly topping off liquid nitrogen, and minimizing temperature fluctuations during transport.
- Transport of liquid semen involves using insulated containers with ice to keep temperatures below 10°C, while frozen semen requires special cryogenic containers and keeping the semen in liquid nitrogen during transport.
- Proper maintenance of liquid nitrogen containers involves regularly checking liquid nitrogen levels, minimizing openings to reduce evaporation, and avoiding thermal and physical damage to
microbiology_sample_collection.pptx
This document provides guidance on specimen management for nursing students. It discusses the importance of proper specimen collection, handling, transportation and storage to ensure quality laboratory test results. Key points include:
- Specimen management is important to reduce laboratory errors and involves proper instructions, collection, handling, transportation and storage.
- Universal precautions should be followed when collecting all specimens to prevent exposure to biohazards. Samples must be properly labeled and packaged for transport.
- The type of specimen and collection method depends on the infection or test being performed. Examples provided include blood, urine, stool and sputum collection procedures.
- Transport media is used to maintain specimen viability during transit to the laboratory and varies based on specimen type. Strict
To study plant tissue culture laboratory design and set up
This document provides guidelines for setting up a plant tissue culture laboratory, including necessary equipment and design considerations. It discusses key areas of the lab such as the culture room, media preparation area, and glassware washing area. Maintaining aseptic conditions is a primary focus of the design. Proper airflow, traffic flow, and separation of clean and dirty areas are emphasized. Necessary equipment includes a laminar hood, autoclave, incubator shaker, and supplies for tissue culture work. Adherence to safety protocols is also covered.
yasir labs designing
The document discusses the requirements and considerations for building and designing various sections of a clinical diagnostic laboratory. It covers architectural and engineering factors like lab module sizes, flexibility for expansion, and placement of utilities. It also describes the specific equipment, instruments, and facilities needed in different diagnostic areas like microbiology, parasitology, pathology, hematology, ELISA testing, PCR, and bacteriology sections. Proper ventilation, security, waste disposal, and health and safety measures are important across all laboratory areas.
Peculiarities of Abattoir and Meat Industry
An abattoir, or slaughterhouse, is a facility that processes animals for food products. Approximately 45-50% of the animal can be used for food, while 15% is waste and 40-45% becomes byproducts. Abattoirs must follow religious practices for slaughtering animals. They come in modern, semi-modern and traditional varieties. Proper site selection, infrastructure, hygiene practices and layout are important for efficient and sanitary operations. Key areas of an abattoir include lairage, slaughtering, processing, inspection, packaging and storage.
Isolation of algae
This document discusses methods for collecting and isolating microalgae samples. It describes collecting algae from water sources using nets or deep water samplers, and from soils using basters or scoops. Five isolation techniques are outlined: 1) micromanipulation using pipettes under a microscope, 2) centrifugal washing to remove bacteria, 3) spraying algae onto agar plates, 4) streak plating using loops, and 5) serial dilution plating in growth media. The document concludes that isolating local algae species can support environmental and industrial uses like biofuel production.
To develop a project for establishment of commercial tissue culture laboratory
The document provides guidelines for establishing a commercial tissue culture laboratory, including:
- The laboratory should have separate rooms for media preparation, glassware washing, sterilization, aseptic transfer, and primary culture growth to maintain cleanliness and prevent contamination.
- Facilities are needed for washing and sterilizing glassware, preparing and storing media, conducting aseptic procedures, and maintaining cultures under controlled conditions.
- Proper location, ventilation, lighting, temperature and humidity control are important considerations for laboratory design.
INSTRUMENTS USED IN MICROBIOLOGY LAB WITH PRINCIPLE AND.ppt
The document discusses various instruments used in microbiology labs, including their principles of operation and common uses. It describes analytical balances, autoclaves, Bunsen burners, centrifuges, colony counters, deep freezers, homogenizers, hot plates, hot air ovens, incubators, laminar flow hoods, magnetic stirrers, microscopes, pH meters, spectrophotometers, vortex mixers, water baths, water distillers, wire loops, Bijoh bottles, glassware, and Durham tubes. It also covers proper collection and transport of clinical specimens for microbiological analysis.
Cell culture
Equipment essential for animal cell culture includes an incubator for maintaining optimal temperature and CO2 levels, a sterile work area like a laminar flow hood, and storage equipment like refrigerators and freezers. Additional beneficial equipment includes microscopes, cell counters, centrifuges, and sterilization tools. Proper cell culture requires maintaining sterile conditions at all times using these various pieces of equipment.
Chicken broiler.pptx
This document provides information on chicken broiler and egg production in the Philippines. It discusses selecting chicken breeds, housing requirements, common diseases, and feeding management. The key points covered are guidelines for selecting foundation stocks, housing specifications based on stock type and production capacity, symptoms and prevention of common poultry diseases like Newcastle disease and fowl pox, and feeding recommendations for broiler and layer chickens at different growth stages.
Seed storage
This document discusses principles and methods for storing seeds to preserve their viability and quality until planting. It outlines the various stages and types of seed storage, as well as factors that affect seed longevity in storage like moisture content, temperature, and pests. Proper storage requires drying seeds to a safe moisture level before placing them in a cool, dry warehouse protected from fluctuations and pests. Cryopreservation in liquid nitrogen at -196°C can also preserve seeds indefinitely by halting biological processes.
Cpcsea guidelines
This document summarizes the CPCSEA (Committee for the Purpose of Control and Supervision of Experiments on Animals) guidelines for animal experimentation. It discusses the goals of humane animal care and avoiding unnecessary pain. It provides recommendations for housing, veterinary care, disease control, and husbandry practices. These include maintaining hygienic facilities, providing social environments, and disposing of waste properly. The document also outlines functions of the CPCSEA like registering facilities, approving protocols, and enforcing compliance. Standard operating procedures, transportation, anesthesia, and euthanasia methods are also addressed to ensure ethical treatment of laboratory animals.
Sample collection, Storage III.ppt
This document provides guidelines for sample collection, storage, transportation and labelling for laboratory testing. Key points include:
- Samples should be properly labelled with animal ID, date, owner details and tests requested.
- Transportation conditions like temperature and containers used must maintain sample integrity.
- Storage conditions vary by sample type but generally include refrigeration, freezing or using transport media.
- Guidelines are provided for collecting different sample types from live/dead animals including blood, serum, tissues and ensuring adequate documentation.
Equipments
Animal cell culture involves removing cells from an animal and growing them in an artificial conductive medium. It is used to produce therapeutic proteins and research diseases. Key equipment for animal cell culture includes biosafety cabinets for sterility, CO2 incubators to maintain temperature and pH, centrifuges to prepare cells, refrigerators/freezers for storage, and microscopes for analysis. Proper equipment is necessary to successfully culture cells outside their natural environment.
Housing management for better productivity.pptx
This document discusses housing management for livestock to improve productivity. It describes two main types of housing systems - loose housing and conventional barns. Loose housing involves keeping animals loose in an open paddock with a common shelter, while conventional barns confine animals together on a platform and secure them with stanchions for individual care and feeding. The document provides advantages and disadvantages of each system and discusses considerations for orientation, space requirements, roofing materials, and sanitation of livestock houses.
P (6) Autoclave and ovens.pdf
The document discusses two common laboratory sterilization instruments: autoclaves and dry ovens. Autoclaves use high-pressure steam to sterilize materials, while dry ovens use dry heat. There are different types of autoclaves including gravity displacement and pre-vacuum models. Sterilization cycles in autoclaves involve purging air, exposing items to high temperature/pressure, and exhausting steam. Dry ovens operate at temperatures over 180°C to kill bacterial spores using hot air, but are not suitable for heat-sensitive items. Proper use, maintenance and packaging are important for effective sterilization with both instruments.
M 1 sterlization
The document discusses various methods of sterilization including physical and chemical methods. Physical sterilization methods include heat (dry and moist), radiation, and filtration. Heat-based methods involve the use of dry heat through processes like flaming or hot air ovens, and moist heat through autoclaving, boiling, or tyndallization. Radiation methods include the use of ionizing radiation like gamma rays and non-ionizing radiation like UV light. Filtration uses filters of varying pore sizes to remove microorganisms from solutions. Chemical sterilization employs chemicals like alcohols, phenols, and oxidizing agents. The document outlines the factors influencing each sterilization method and their appropriate applications.
Similar to planning of semen bank.pptx (20)
To study plant tissue culture laboratory design and set up
To study plant tissue culture laboratory design and set up
To develop a project for establishment of commercial tissue culture laboratory
To develop a project for establishment of commercial tissue culture laboratory
INSTRUMENTS USED IN MICROBIOLOGY LAB WITH PRINCIPLE AND.ppt
INSTRUMENTS USED IN MICROBIOLOGY LAB WITH PRINCIPLE AND.ppt
More from BabuMGowda1
Handling of LN2.pptx
This document discusses the handling of cryogenic containers containing frozen semen for artificial insemination. It outlines the necessary equipment, including liquid nitrogen containers, dip sticks, artificial insemination guns, plastic sheaths, and forceps. It describes the process of thawing frozen semen straws and loading the straw into an AI gun with a plastic sheath for insemination. Safety precautions are provided for transporting and working with cryogenic containers to safely preserve frozen biological materials like semen.
breeding soundness exam in bulls.pptx
Breeding soundness evaluation (BSE) assesses a bull's reliability and capability for breeding through physical examination and evaluation of semen quality, libido, and freedom from disease. The evaluation has several components: physical exam; examination of external genitalia and reproductive organs; semen evaluation for motility, morphology, and infectious agents; and tests of mating ability and libido. Bulls are classified as satisfactory, deferred, or unsatisfactory breeders based on their scores on factors like scrotal circumference, semen motility, and morphology. Regular BSE can increase pregnancy rates by eliminating less fertile bulls.
estrus detection aids.pptx
This document discusses various methods of estrus detection in farm animals. It begins by defining estrus as the period when female animals become sexually receptive. Accurate estrus detection is important for achieving optimal fertility and conception rates. Common methods mentioned include using identification tags or markings and keeping daily records through heat expectancy charts, breeding wheels, or computer programs. Additional aids discussed are mount detectors, tailhead markings, activity monitors, and vaginal probes. Regular observation and record keeping are emphasized as the foundation for a successful reproductive program.
record keeping.pptx
1. Farm animal health and breeding records are important for evaluating reproductive performance, selecting and culling animals, and implementing breeding programs.
2. Records can be kept manually in notebooks or on index cards, or electronically on computer systems. They include information like estrus detection, breeding dates, pregnancy checks, calving events, milk production, health treatments, and vaccinations.
3. Veterinarians work with farmers and herdsmen to review animal records during farm visits in order to monitor herd health, fertility, and provide recommendations. Regular record keeping and communication between the veterinarian and farm manager is important for effective herd management.
caesarean operation.pptx
This document provides information on performing a caesarean section in cattle. It discusses the necessary supplies, potential indications for the surgery including fetal issues and birthing difficulties. It describes three common positions for the surgery - standing, dorsal recumbency, and lateral recumbency. It then details the steps of the surgery including anesthetizing the animal, making the incision, exteriorizing and delivering the fetus, closing the uterine incision, and post-operative care. Common surgical approaches and complications are also summarized.
BCS.pptx
Body condition scoring involves visually assessing and handling cows to determine their fat reserves based on a 1-5 point scale. It is conducted at key stages of lactation, including before drying off and calving, to ensure cows are in the ideal condition and fertility is not compromised. Regular scoring allows adjustments to feeding to maintain targets of 2.75-3 at calving and >2.5-2.75 at peak lactation, linking cow condition to milk production and reproductive health.
synchronization and superovulation.pptx
This document discusses synchronization of estrus and superovulation in cattle. It describes:
1) The objectives of synchronization to create a uniform calf crop through timed AI.
2) Hormones used for synchronization including prostaglandins and protocols using prostaglandins alone or with progesterone, estrogens or GnRH.
3) Superovulation objectives to increase embryo yields for transfer and hormones used including FSH, eCG and protocols for cattle, sheep and goats.
4) Factors influencing superovulatory response related to the dominant follicle and nutrition/lactation.
CRYOPRESERVATION.pptx
Cryopreservation is the use of very low temperatures to preserve living cells and tissue. Cryoprotectants like glycerol and DMSO are used to reduce ice formation and allow cells to withstand freezing. They work by penetrating cells, lowering the freezing point, and minimizing solution effects. Glycerol is often used for most cell types due to lower toxicity, while DMSO is preferred for larger cells. Cryoprotectants must be soluble, able to penetrate cells, and minimally toxic at high concentrations required for effective cryopreservation.
vaginal cytology in bitches.pptx
This document discusses vaginal cytology, which is the study of the structure, shape, and contour of exfoliated vaginal epithelial cells using staining techniques. Vaginal cytology can help characterize a bitch's estrus cycle to determine the best time for breeding, evaluate any diseases of the genital tract like vaginitis or pyometra, and identify some neoplastic conditions of the vagina and urethra such as transmissible vaginal tumors.
faulty fetal disposition.pptx
This document discusses different fetal positions during childbirth. It identifies three main types of fetal positions: longitudinal where the baby's length is aligned with the mother's body, transverse where the baby is lying sideways, and vertical where the baby is standing upright. It further defines two sub-types of longitudinal presentation as dorso-sacral where the baby's back is to the mother's sacrum and dorso-pubic where the baby's back is to the mother's pubic bone.
follicular dynamics ppt.pptx
The ovary has two main functions: gametogenesis, which is the cyclic production of fertilizable ova, and steroidogenesis, which is the production of steroid hormones. Folliculogenesis is the process by which mature follicles capable of ovulation develop from primordial follicles in the ovary. It involves several stages: primary follicle, secondary follicle, tertiary follicle, and graafian or antral follicle. Follicular growth occurs in waves, with each wave culminating in the formation of a large follicle. Factors like diet, age, parity, and lactation status can influence the number of waves per estrous cycle, which is usually two waves for lactating dairy cows and three waves for nullipar
infertility.pptx
This document describes common reproductive tract disorders in cattle, including ovarian hypoplasia, uterus unicornis, uterine didelphys, hydrometra, oophoritis, granulose cell tumor, ovariobursal adhesions, follicular cysts, luteal cysts, paraovarian cysts, hydrosalpinx, pyometra, cystic endometrial hyperplasia, mucometra, cervicitis, fetal mummification, and fetal maceration. It provides details on the pathogenesis, clinical signs, and diagnosis of each condition.
sperm abnormalities.pptx
The document compares the dimensions of bull and buffalo sperm. Bull sperm have a longer, narrower head and a longer, thinner midpiece than buffalo sperm. The head and midpiece are parts of sperm that were measured in microns and compared between bulls and buffalos.
pregnancy diagnosis.pptx
This document discusses various methods for diagnosing pregnancy in livestock, including management, clinical, radiographic, ultrasound, and laboratory-based methods. Clinical methods include rectal palpation to check for uterine horn enlargement, fetal membranes, cotyledons, and fetal bump. Ultrasound uses a linear rectal probe to diagnose cow pregnancy. Laboratory methods involve analyzing blood, milk, or urine samples to detect pregnancy hormones like progesterone. Differential diagnosis considers conditions that could be confused for pregnancy, such as tumors, mummified fetuses, or uterine infections.
CRYOBIOLOGY.pptx
Cryopreservation is a process that preserves cells, tissues, or other substances by cooling them to very low sub-zero temperatures, usually below -150°C. This prevents damage from chemical reactions or the passage of time by effectively stopping all enzymatic or chemical activity. Cryopreservation methods seek to reach these low temperatures without additional damage caused by the formation of ice during freezing. Traditionally, the material is coated with cryoprotectant molecules to preserve it through freezing and thawing.
More from BabuMGowda1 (15)
Recently uploaded
Adhd Medication Shortage Uk - trinexpharmacy.com
The UK is currently facing a Adhd Medication Shortage Uk, which has left many patients and their families grappling with uncertainty and frustration. ADHD, or Attention Deficit Hyperactivity Disorder, is a chronic condition that requires consistent medication to manage effectively. This shortage has highlighted the critical role these medications play in the daily lives of those affected by ADHD. Contact : +1 (747) 209 – 3649 E-mail : sales@trinexpharmacy.com
Top Effective Soaps for Fungal Skin Infections in India
Swisschem Dermacare has mentioned the List of The Best Antifungal Soap In India 2022. All of these soaps are trusted by various Dermatology Experts.
The Electrocardiogram - Physiologic Principles
These lecture slides, by Dr Sidra Arshad, offer a quick overview of the physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar lead (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
6. Describe the flow of current around the heart during the cardiac cycle
7. Discuss the placement and polarity of the leads of electrocardiograph
8. Describe the normal electrocardiograms recorded from the limb leads and explain the physiological basis of the different records that are obtained
9. Define mean electrical vector (axis) of the heart and give the normal range
10. Define the mean QRS vector
11. Describe the axes of leads (hexagonal reference system)
12. Comprehend the vectorial analysis of the normal ECG
13. Determine the mean electrical axis of the ventricular QRS and appreciate the mean axis deviation
14. Explain the concepts of current of injury, J point, and their significance
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. Chapter 3, Cardiology Explained, https://www.ncbi.nlm.nih.gov/books/NBK2214/
7. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
REGULATION FOR COMBINATION PRODUCTS AND MEDICAL DEVICES.pptx
It includes regulation of combination products and medical devices. FDA and industry liaisons.
Clinic ^%[+27633867063*Abortion Pills For Sale In Tembisa Central
Clinic ^%[+27633867063*Abortion Pills For Sale In Tembisa Central Clinic ^%[+27633867063*Abortion Pills For Sale In Tembisa CentralClinic ^%[+27633867063*Abortion Pills For Sale In Tembisa CentralClinic ^%[+27633867063*Abortion Pills For Sale In Tembisa CentralClinic ^%[+27633867063*Abortion Pills For Sale In Tembisa Central
A Classical Text Review on Basavarajeeyam
Basavarajeeyam is a Sreshta Sangraha grantha (Compiled book ), written by Neelkanta kotturu Basavaraja Virachita. It contains 25 Prakaranas, First 24 Chapters related to Rogas& 25th to Rasadravyas.
TEST BANK For An Introduction to Brain and Behavior, 7th Edition by Bryan Kol...
TEST BANK For An Introduction to Brain and Behavior, 7th Edition by Bryan Kol...rightmanforbloodline
TEST BANK For An Introduction to Brain and Behavior, 7th Edition by Bryan Kolb, Ian Q. Whishaw, Verified Chapters 1 - 16, Complete Newest Versio
TEST BANK For An Introduction to Brain and Behavior, 7th Edition by Bryan Kolb, Ian Q. Whishaw, Verified Chapters 1 - 16, Complete Newest Version
TEST BANK For An Introduction to Brain and Behavior, 7th Edition by Bryan Kolb, Ian Q. Whishaw, Verified Chapters 1 - 16, Complete Newest VersionRole of Mukta Pishti in the Management of Hyperthyroidism
Muktapishti is a traditional Ayurvedic preparation made from Shoditha Mukta (Purified Pearl), is believed to help regulate thyroid function and reduce symptoms of hyperthyroidism due to its cooling and balancing properties. Clinical evidence on its efficacy remains limited, necessitating further research to validate its therapeutic benefits.
Cell Therapy Expansion and Challenges in Autoimmune Disease
There is increasing confidence that cell therapies will soon play a role in the treatment of autoimmune disorders, but the extent of this impact remains to be seen. Early readouts on autologous CAR-Ts in lupus are encouraging, but manufacturing and cost limitations are likely to restrict access to highly refractory patients. Allogeneic CAR-Ts have the potential to broaden access to earlier lines of treatment due to their inherent cost benefits, however they will need to demonstrate comparable or improved efficacy to established modalities.
In addition to infrastructure and capacity constraints, CAR-Ts face a very different risk-benefit dynamic in autoimmune compared to oncology, highlighting the need for tolerable therapies with low adverse event risk. CAR-NK and Treg-based therapies are also being developed in certain autoimmune disorders and may demonstrate favorable safety profiles. Several novel non-cell therapies such as bispecific antibodies, nanobodies, and RNAi drugs, may also offer future alternative competitive solutions with variable value propositions.
Widespread adoption of cell therapies will not only require strong efficacy and safety data, but also adapted pricing and access strategies. At oncology-based price points, CAR-Ts are unlikely to achieve broad market access in autoimmune disorders, with eligible patient populations that are potentially orders of magnitude greater than the number of currently addressable cancer patients. Developers have made strides towards reducing cell therapy COGS while improving manufacturing efficiency, but payors will inevitably restrict access until more sustainable pricing is achieved.
Despite these headwinds, industry leaders and investors remain confident that cell therapies are poised to address significant unmet need in patients suffering from autoimmune disorders. However, the extent of this impact on the treatment landscape remains to be seen, as the industry rapidly approaches an inflection point.
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...
Overall life span (LS) was 1671.7±1721.6 days and cumulative 5YS reached 62.4%, 10 years – 50.4%, 20 years – 44.6%. 94 LCP lived more than 5 years without cancer (LS=2958.6±1723.6 days), 22 – more than 10 years (LS=5571±1841.8 days). 67 LCP died because of LC (LS=471.9±344 days). AT significantly improved 5YS (68% vs. 53.7%) (P=0.028 by log-rank test). Cox modeling displayed that 5YS of LCP significantly depended on: N0-N12, T3-4, blood cell circuit, cell ratio factors (ratio between cancer cells-CC and blood cells subpopulations), LC cell dynamics, recalcification time, heparin tolerance, prothrombin index, protein, AT, procedure type (P=0.000-0.031). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and N0-12 (rank=1), thrombocytes/CC (rank=2), segmented neutrophils/CC (3), eosinophils/CC (4), erythrocytes/CC (5), healthy cells/CC (6), lymphocytes/CC (7), stick neutrophils/CC (8), leucocytes/CC (9), monocytes/CC (10). Correct prediction of 5YS was 100% by neural networks computing (error=0.000; area under ROC curve=1.0).
Promoting Wellbeing - Applied Social Psychology - Psychology SuperNotes
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
8 Surprising Reasons To Meditate 40 Minutes A Day That Can Change Your Life.pptx
8 Surprising Reasons To Meditate 40 Minutes A Day That Can Change Your Life.pptxHolistified Wellness
We’re talking about Vedic Meditation, a form of meditation that has been around for at least 5,000 years. Back then, the people who lived in the Indus Valley, now known as India and Pakistan, practised meditation as a fundamental part of daily life. This knowledge that has given us yoga and Ayurveda, was known as Veda, hence the name Vedic. And though there are some written records, the practice has been passed down verbally from generation to generation.The Best Ayurvedic Antacid Tablets in India
Treat the symptoms of indigestion, heartburn and stomach reflux with the 10 Best Ayurvedic Antacid Tablets in India.
Osteoporosis - Definition , Evaluation and Management .pdf
Osteoporosis is an increasing cause of morbidity among the elderly.
In this document , a brief outline of osteoporosis is given , including the risk factors of osteoporosis fractures , the indications for testing bone mineral density and the management of osteoporosis
Recently uploaded (20)
Top Effective Soaps for Fungal Skin Infections in India
Top Effective Soaps for Fungal Skin Infections in India
REGULATION FOR COMBINATION PRODUCTS AND MEDICAL DEVICES.pptx
REGULATION FOR COMBINATION PRODUCTS AND MEDICAL DEVICES.pptx
Clinic ^%[+27633867063*Abortion Pills For Sale In Tembisa Central
Clinic ^%[+27633867063*Abortion Pills For Sale In Tembisa Central
TEST BANK For An Introduction to Brain and Behavior, 7th Edition by Bryan Kol...
TEST BANK For An Introduction to Brain and Behavior, 7th Edition by Bryan Kol...
Role of Mukta Pishti in the Management of Hyperthyroidism
Role of Mukta Pishti in the Management of Hyperthyroidism
Cell Therapy Expansion and Challenges in Autoimmune Disease
Cell Therapy Expansion and Challenges in Autoimmune Disease
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...
Promoting Wellbeing - Applied Social Psychology - Psychology SuperNotes
Promoting Wellbeing - Applied Social Psychology - Psychology SuperNotes
8 Surprising Reasons To Meditate 40 Minutes A Day That Can Change Your Life.pptx
8 Surprising Reasons To Meditate 40 Minutes A Day That Can Change Your Life.pptx
Osteoporosis - Definition , Evaluation and Management .pdf
Osteoporosis - Definition , Evaluation and Management .pdf
planning of semen bank.pptx
- 2. Introduction
- 3. Objectives • To know the different components of a semen bank. • To know the important factors to be considered while constructing a semen bank. • To acquaint with the equipments required in a semen bank.
- 4. 1. Semen collection yard 2. Semen evaluation and processing laboratory 3. Quality control/Assurance lab 4. Frozen semen storage area 5. Store for feed and fodders 6. Bull shed 7. Protected open area for exercise with facility of bull exerciser 8. Segregation ward for sick animals 9. Quarantine shed for newly introducing animals 10. Residential quarters
- 8. Two row system of housing should be in N-W to S-E direction The bull shed area 16 ft x 6 ft open yard 16 ft x20 ft ceiling 10-12 ft height ventilation 3-4 ft gap between roof & ventilator
- 9. Do’s: 1. Should be next to the bull shed 2. Should have a floor space of 8x9 meters which can accommodate two service crates . 3. Floor should be non-slippery
- 10. Dont’s: 1. Direct exposure to sunlight, dirt cold and hot wind and also from rain. 2. Overcrowding
- 11. • Should be close to the collection shed • Should have restricted entry • Should have a. Ante room, b. Sterilization room, c. Washing room, d. AV room, e. Buffer and diluent preparation room, f. evaluation and processing room, g. freezing room, h. office cum record room, i. store room and computer room
- 13. The frozen semen should be evaluated for the quality after 24 hours of initial storage and again after 30 days of quarantine before distribution to the AI centers
- 14. • Store sufficient feed for lean season. • The floor should be free from moisture to avoid spoilage of feed/fodder; the concentrate feed should be kept over the wooden flanks at least 6” above the floor. • The entry of rats and bandicoots should be prevented.
- 15. Sick bulls are housed for regular treatment
- 16. • The bulls should be given daily regular exercise in bull exerciser to maintain its health and kept in a place near to the bull sheds
- 17. • A separate quarantine shed for the newly introduced bulls for daily observation and screening for diseases before allowing for collection.
- 18. • Phase contrast microscope with stage warmer • Laminar air flow • Accucell photometer/Calorimeter • Pipettes with tips • Test tube stand • Water bath • Semen dilutor • Sugar tubes • Refrigerator • Air conditioner • Wide mouth container • Freezing racks
- 19. • Magnetic stirrer • Conical flask, measuring cylinder • Glass distillation unit/Millipore water apparatus • Glass funnels • Plastic trays • Enamel trays • Nose rings for bulls • Nose punch for bulls
- 20. • Instrument cabinet to store the sterilized materials • UV lamb for sterilization • Ethylene oxide sterilizer • Autoclave • Hot air oven • Artificial vagina • Graduated semen collection vials • Bio-freezer • Cold handling cabinet • Aluminum foil • Haemocytometer
- 21. • Glass slides, cover slips • Spirit lamp • pH meter • Watch glasses • Test tubes • Filter paper • Forceps • Gum boots • Thermometer