⦿ This document discusses key considerations for establishing a successful tissue culture laboratory. It addresses the importance of strategic planning, proper location, and laboratory design to maintain aseptic conditions. A standard tissue culture lab should have areas for media preparation, sterilization, glassware washing, storage, aseptic transfer, and a primary growth room with environmental controls. Careful attention to traffic flow, lighting, temperature, humidity and other factors can optimize the lab's function. Networking, quality control, and market research are also important for a profitable tissue culture business.
Seed Bank Design: Seed Drying Rooms
`
For more information, Please see websites below:
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Organic Edible Schoolyards & Gardening with Children =
http://scribd.com/doc/239851214 ~
`
Double Food Production from your School Garden with Organic Tech =
http://scribd.com/doc/239851079 ~
`
Free School Gardening Art Posters =
http://scribd.com/doc/239851159 ~
`
Increase Food Production with Companion Planting in your School Garden =
http://scribd.com/doc/239851159 ~
`
Healthy Foods Dramatically Improves Student Academic Success =
http://scribd.com/doc/239851348 ~
`
City Chickens for your Organic School Garden =
http://scribd.com/doc/239850440 ~
`
Simple Square Foot Gardening for Schools - Teacher Guide =
http://scribd.com/doc/239851110 ~
Weiber manufactures various types of controlled environment chambers for agricultural research, including greenhouses, growth chambers, climate chambers, and specialized chambers for experiments on plants, insects, seeds, and more. The chambers precisely control temperature, humidity, air, lighting, and other environmental factors to enable research and testing under different environmental conditions without regard for natural outdoor variations. Weiber offers customized chamber solutions and a wide range of standard chamber models tailored for different research applications.
Bentall Rowlands Storage Systems Limited is a leading UK manufacturer in complete storage and processing equipment solutions for the agricultural and industrial markets.
EnviroChemie offers thermal wastewater sterilization systems called SteriFix that use high temperatures to inactivate pathogens and contaminants in wastewater. SteriFix systems are designed for decentralized or continuous wastewater treatment for volumes from 100L to 20m3 daily. They reliably sterilize wastewater through precisely controlled thermal processes and have over 35 years of industry experience and reference installations.
Shaker incubator manufacturer in chennai|india|bangalore|hyderabad|pune|delhi...velswamy
The Top Roofing contractors in Chennai are Sole Proprietorship (Individual) based firm, engaged as the trader and wholesaler of Roofing Contractors, Roofing Shed, Terrace Roofing and many more. Our Unique Selling Point is providing the best quality and Durable Goods in Reasonable Price.
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Types of incubators parts and their operationsAshiq Toor
This document discusses different types of incubators and their operations. It describes still air incubators which do not have fans and forced air incubators which circulate air using internal fans. It also discusses basic incubators, advanced incubators, cabinet incubators, and multi-setting incubators. Multi-setting incubators provide different environmental conditions for different egg development stages. The document focuses on the Chick Master incubator system as an example and describes its laminar air flow system and multi-zone controls.
The document discusses various advances in seed storage methods. It notes that proper storage is important to maintain seed quality until planting. Methods discussed include drying seeds to safe moisture levels, using structurally sound storage structures in cool areas, hermetic bags, metal bins, treated jute and paper bags, and modern fumigation techniques using gases like methyl bromide to kill insects in stored seeds and grains. Good storage practices involve cleaning, pest control, and regular inspection and treatment if needed.
Seed Bank Design: Seed Drying Rooms
`
For more information, Please see websites below:
`
Organic Edible Schoolyards & Gardening with Children =
http://scribd.com/doc/239851214 ~
`
Double Food Production from your School Garden with Organic Tech =
http://scribd.com/doc/239851079 ~
`
Free School Gardening Art Posters =
http://scribd.com/doc/239851159 ~
`
Increase Food Production with Companion Planting in your School Garden =
http://scribd.com/doc/239851159 ~
`
Healthy Foods Dramatically Improves Student Academic Success =
http://scribd.com/doc/239851348 ~
`
City Chickens for your Organic School Garden =
http://scribd.com/doc/239850440 ~
`
Simple Square Foot Gardening for Schools - Teacher Guide =
http://scribd.com/doc/239851110 ~
Weiber manufactures various types of controlled environment chambers for agricultural research, including greenhouses, growth chambers, climate chambers, and specialized chambers for experiments on plants, insects, seeds, and more. The chambers precisely control temperature, humidity, air, lighting, and other environmental factors to enable research and testing under different environmental conditions without regard for natural outdoor variations. Weiber offers customized chamber solutions and a wide range of standard chamber models tailored for different research applications.
Bentall Rowlands Storage Systems Limited is a leading UK manufacturer in complete storage and processing equipment solutions for the agricultural and industrial markets.
EnviroChemie offers thermal wastewater sterilization systems called SteriFix that use high temperatures to inactivate pathogens and contaminants in wastewater. SteriFix systems are designed for decentralized or continuous wastewater treatment for volumes from 100L to 20m3 daily. They reliably sterilize wastewater through precisely controlled thermal processes and have over 35 years of industry experience and reference installations.
Shaker incubator manufacturer in chennai|india|bangalore|hyderabad|pune|delhi...velswamy
The Top Roofing contractors in Chennai are Sole Proprietorship (Individual) based firm, engaged as the trader and wholesaler of Roofing Contractors, Roofing Shed, Terrace Roofing and many more. Our Unique Selling Point is providing the best quality and Durable Goods in Reasonable Price.
seo
https://bit.ly/30cp4dK
https://bit.ly/2zaIa9g
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https://seotechsolution.in/
https://bit.ly/2YbSFTx
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reliance
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vibromag
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https://bit.ly/3gjaRSS
https://bit.ly/3vtgdk1
https://bit.ly/2TsPMh4
https://bit.ly/3pDp4ya
Types of incubators parts and their operationsAshiq Toor
This document discusses different types of incubators and their operations. It describes still air incubators which do not have fans and forced air incubators which circulate air using internal fans. It also discusses basic incubators, advanced incubators, cabinet incubators, and multi-setting incubators. Multi-setting incubators provide different environmental conditions for different egg development stages. The document focuses on the Chick Master incubator system as an example and describes its laminar air flow system and multi-zone controls.
The document discusses various advances in seed storage methods. It notes that proper storage is important to maintain seed quality until planting. Methods discussed include drying seeds to safe moisture levels, using structurally sound storage structures in cool areas, hermetic bags, metal bins, treated jute and paper bags, and modern fumigation techniques using gases like methyl bromide to kill insects in stored seeds and grains. Good storage practices involve cleaning, pest control, and regular inspection and treatment if needed.
Hot air ovens use dry heat between 50 to 300 degrees Celsius to sterilize items, with a double-walled insulation and circulating fan helping to uniformly distribute heat inside while conserving energy. They were originally developed by Pasteur and include adjustable trays, temperature and time controls, with efficacy tested using biological indicators during a complete cycle of heating, maintaining temperature for a set time, and cooling contents.
International Journal of Computational Engineering Research(IJCER) is an intentional online Journal in English monthly publishing journal. This Journal publish original research work that contributes significantly to further the scientific knowledge in engineering and Technology.
This document outlines the pre-brooding management practices for a poultry farm. It discusses selecting and preparing the shed, removing litter, cleaning processes, making repairs, installing equipment like feeders and sensors, and fumigating. The goal is to avoid disease, remove pathogens, and get the farm ready to receive new chicks. Calculations are provided for materials needed like disinfectants, litter, and equipment based on the size of the shed. Thorough cleaning and disinfection of the farm is essential before the chicks arrive.
Hot Air Oven, Laboratory Incubator, Bacteriological IncubatorSmita Scientific
Smita Scientific is a manufacturer and supplier of laboratory equipment established in 2006. They offer a range of products including laboratory ovens, incubators, hot plates, water baths, and digestion and distillation units. The company has a team of qualified professionals and a sound infrastructure to help meet production targets and clients' needs. Smita Scientific prides itself on using high-quality materials and an R&D process to manufacture reliable, durable equipment in various sizes.
Freeze Drying is the process of removing the moisture from a biological product while maintaining the Integrity of the products biological & chemical structure and activities.
This document provides recommendations for integrated pest management (IPM) in various areas. It identifies key areas to inspect that are prone to pest infestations, such as warehouses, trash rooms, kitchens, restrooms, and outdoor areas. Specific recommendations are given to seal cracks and crevices, clean drains, wash garbage cans, install air curtains at entrances, remove standing water and old tires, rodent-proof doors, clean under equipment, and properly store mops to avoid attracting and harboring various pests. Maintaining cleanliness and sanitation in facilities is emphasized.
Daftar Harga Pemanas Air Water Heater Ariston Pemanas Air Tenaga Listrik Kapasitas 15,30,50,80,100 Liter Tipe Pro R - RS - AN R - Shape ECO - TI Pro - Slim Harga Murah Bergaransi Resmi dari Ariston Harga Sudah Pasang Cara Pembayaran Bisa COD atau bayar di tempat dan setelah terpasang Hubungi Kami Sekarang ?
This document provides guidelines for designing an effective plant tissue culture laboratory. Careful strategic planning is needed when designing or remodeling a lab space. Key areas that are addressed include the location, general laboratory design, glassware washing and storage, media preparation and sterilization, the primary growth room, and the aseptic transfer area. Maintaining aseptic conditions, controlled environmental factors, and an efficient workflow are priorities in the lab design.
To develop a project for establishment of commercial tissue culture laboratoryHORTIPEDIA INDIA
The document provides guidelines for establishing a commercial tissue culture laboratory, including:
- The laboratory should have separate rooms for media preparation, glassware washing, sterilization, aseptic transfer, and primary culture growth to maintain cleanliness and prevent contamination.
- Facilities are needed for washing and sterilizing glassware, preparing and storing media, conducting aseptic procedures, and maintaining cultures under controlled conditions.
- Proper location, ventilation, lighting, temperature and humidity control are important considerations for laboratory design.
To study plant tissue culture laboratory design and set upHORTIPEDIA INDIA
This document provides guidelines for setting up a plant tissue culture laboratory, including necessary equipment and design considerations. It discusses key areas of the lab such as the culture room, media preparation area, and glassware washing area. Maintaining aseptic conditions is a primary focus of the design. Proper airflow, traffic flow, and separation of clean and dirty areas are emphasized. Necessary equipment includes a laminar hood, autoclave, incubator shaker, and supplies for tissue culture work. Adherence to safety protocols is also covered.
The document provides guidance on designing seed drying rooms for seed banks. Key points include:
- Drying seeds prior to storage is important to prolong lifespan and prevent germination. The ideal is 15% relative humidity at 15°C.
- A purpose-built drying room allows large quantities of seeds to dry simultaneously and provides storage space. The size depends on the volume of seeds but is typically 25-125 cubic meters.
- Proper insulation, airflow, temperature control and monitoring equipment are needed to maintain the optimal drying conditions for seeds. Technical expertise is required to design an effective drying room.
Equipment essential for animal cell culture includes an incubator for maintaining optimal temperature and CO2 levels, a sterile work area like a laminar flow hood, and storage equipment like refrigerators and freezers. Additional beneficial equipment includes microscopes, cell counters, centrifuges, and sterilization tools. Proper cell culture requires maintaining sterile conditions at all times using these various pieces of equipment.
This document discusses the key considerations for designing a successful plant micropropagation laboratory. It emphasizes that careful planning of facilities, location, and equipment is important. The ideal lab design includes separate areas for glassware washing and storage, media preparation and sterilization, aseptic transfer under laminar flow hoods, and a primary growth room that maintains optimal temperature, lighting, and humidity for plant cultivation. Proper environmental controls and isolation of lab areas are vital for achieving aseptic conditions needed for micropropagation.
The document outlines the key considerations for designing an effective plant tissue culture laboratory. It discusses that the laboratory should have separate areas for washing and storage, media preparation, aseptic transfer, and primary growth. The aseptic transfer area and primary growth room require stringent cleanliness, with HEPA filters, UV lights, and laminar flow hoods. Other areas require equipment for media preparation, sterilization, and storage. The primary growth room needs temperature, humidity, and lighting controls to suit the plants being cultured. Careful planning of traffic flow and separation of clean and dirty areas is important for success.
The title of this presentation is :-Establishment of animal tissue culture la...KESHAVPOSWAL
This presentation indicates that how to establish the animal tissue culture lab , what are the necessary conditions that should be taken into consideration while designing the animal tissue culture lab layout. It also emphasize on various instruments that generally used in any tissue culture lab.
Plant tissue culture is a trending and a promising area due to its applications. hence knowledge of its basics and history would be helpful for the pioneers. ans this presentation also discusses about the structure and establishment of a commercial tissue culture laboratory.
The document discusses the requirements and considerations for building and designing various sections of a clinical diagnostic laboratory. It covers architectural and engineering factors like lab module sizes, flexibility for expansion, and placement of utilities. It also describes the specific equipment, instruments, and facilities needed in different diagnostic areas like microbiology, parasitology, pathology, hematology, ELISA testing, PCR, and bacteriology sections. Proper ventilation, security, waste disposal, and health and safety measures are important across all laboratory areas.
Introduction and history, basic requirements forof PTC.pptxSujata Koundal
This document discusses plant tissue culture. It begins by defining plant tissue culture as the in vitro cultivation of plant cells or tissues under aseptic conditions. It then describes the basic requirements for a plant tissue culture laboratory, including equipment for media preparation, sterilization, culture incubation, and data collection. The document outlines general procedures for plant tissue culture, such as sterilizing glassware and explants, producing callus from explants, proliferating and sub-culturing callus, and creating suspension cultures. It provides details on surface sterilization methods and media used at different stages of culture.
Laboratory designed notes. For studyingElias910858
This document provides guidelines for designing laboratory spaces safely. It addresses considerations for general laboratory design including area layout, ergonomics, maintainability, infrastructure, and more. Specific guidance is provided for electrical systems, biosafety cabinets, ventilation, gas systems, waste handling, and other laboratory equipment and features. The document emphasizes compliance with codes and involving Environmental Health and Safety in the design process to ensure all laboratory needs and hazards are appropriately addressed.
The document lists and describes various laboratory equipment used for the mass production of biofertilizers, including autoclaves, hot air ovens, laminar air flow hoods, and other equipment. It provides details on the components, working principles, and uses of autoclaves, hot air ovens, and laminar air flow hoods, which are key pieces of equipment for sterilizing media, containers, and maintaining sterile environments for biofertilizer production.
Hot air ovens use dry heat between 50 to 300 degrees Celsius to sterilize items, with a double-walled insulation and circulating fan helping to uniformly distribute heat inside while conserving energy. They were originally developed by Pasteur and include adjustable trays, temperature and time controls, with efficacy tested using biological indicators during a complete cycle of heating, maintaining temperature for a set time, and cooling contents.
International Journal of Computational Engineering Research(IJCER) is an intentional online Journal in English monthly publishing journal. This Journal publish original research work that contributes significantly to further the scientific knowledge in engineering and Technology.
This document outlines the pre-brooding management practices for a poultry farm. It discusses selecting and preparing the shed, removing litter, cleaning processes, making repairs, installing equipment like feeders and sensors, and fumigating. The goal is to avoid disease, remove pathogens, and get the farm ready to receive new chicks. Calculations are provided for materials needed like disinfectants, litter, and equipment based on the size of the shed. Thorough cleaning and disinfection of the farm is essential before the chicks arrive.
Hot Air Oven, Laboratory Incubator, Bacteriological IncubatorSmita Scientific
Smita Scientific is a manufacturer and supplier of laboratory equipment established in 2006. They offer a range of products including laboratory ovens, incubators, hot plates, water baths, and digestion and distillation units. The company has a team of qualified professionals and a sound infrastructure to help meet production targets and clients' needs. Smita Scientific prides itself on using high-quality materials and an R&D process to manufacture reliable, durable equipment in various sizes.
Freeze Drying is the process of removing the moisture from a biological product while maintaining the Integrity of the products biological & chemical structure and activities.
This document provides recommendations for integrated pest management (IPM) in various areas. It identifies key areas to inspect that are prone to pest infestations, such as warehouses, trash rooms, kitchens, restrooms, and outdoor areas. Specific recommendations are given to seal cracks and crevices, clean drains, wash garbage cans, install air curtains at entrances, remove standing water and old tires, rodent-proof doors, clean under equipment, and properly store mops to avoid attracting and harboring various pests. Maintaining cleanliness and sanitation in facilities is emphasized.
Daftar Harga Pemanas Air Water Heater Ariston Pemanas Air Tenaga Listrik Kapasitas 15,30,50,80,100 Liter Tipe Pro R - RS - AN R - Shape ECO - TI Pro - Slim Harga Murah Bergaransi Resmi dari Ariston Harga Sudah Pasang Cara Pembayaran Bisa COD atau bayar di tempat dan setelah terpasang Hubungi Kami Sekarang ?
This document provides guidelines for designing an effective plant tissue culture laboratory. Careful strategic planning is needed when designing or remodeling a lab space. Key areas that are addressed include the location, general laboratory design, glassware washing and storage, media preparation and sterilization, the primary growth room, and the aseptic transfer area. Maintaining aseptic conditions, controlled environmental factors, and an efficient workflow are priorities in the lab design.
To develop a project for establishment of commercial tissue culture laboratoryHORTIPEDIA INDIA
The document provides guidelines for establishing a commercial tissue culture laboratory, including:
- The laboratory should have separate rooms for media preparation, glassware washing, sterilization, aseptic transfer, and primary culture growth to maintain cleanliness and prevent contamination.
- Facilities are needed for washing and sterilizing glassware, preparing and storing media, conducting aseptic procedures, and maintaining cultures under controlled conditions.
- Proper location, ventilation, lighting, temperature and humidity control are important considerations for laboratory design.
To study plant tissue culture laboratory design and set upHORTIPEDIA INDIA
This document provides guidelines for setting up a plant tissue culture laboratory, including necessary equipment and design considerations. It discusses key areas of the lab such as the culture room, media preparation area, and glassware washing area. Maintaining aseptic conditions is a primary focus of the design. Proper airflow, traffic flow, and separation of clean and dirty areas are emphasized. Necessary equipment includes a laminar hood, autoclave, incubator shaker, and supplies for tissue culture work. Adherence to safety protocols is also covered.
The document provides guidance on designing seed drying rooms for seed banks. Key points include:
- Drying seeds prior to storage is important to prolong lifespan and prevent germination. The ideal is 15% relative humidity at 15°C.
- A purpose-built drying room allows large quantities of seeds to dry simultaneously and provides storage space. The size depends on the volume of seeds but is typically 25-125 cubic meters.
- Proper insulation, airflow, temperature control and monitoring equipment are needed to maintain the optimal drying conditions for seeds. Technical expertise is required to design an effective drying room.
Equipment essential for animal cell culture includes an incubator for maintaining optimal temperature and CO2 levels, a sterile work area like a laminar flow hood, and storage equipment like refrigerators and freezers. Additional beneficial equipment includes microscopes, cell counters, centrifuges, and sterilization tools. Proper cell culture requires maintaining sterile conditions at all times using these various pieces of equipment.
This document discusses the key considerations for designing a successful plant micropropagation laboratory. It emphasizes that careful planning of facilities, location, and equipment is important. The ideal lab design includes separate areas for glassware washing and storage, media preparation and sterilization, aseptic transfer under laminar flow hoods, and a primary growth room that maintains optimal temperature, lighting, and humidity for plant cultivation. Proper environmental controls and isolation of lab areas are vital for achieving aseptic conditions needed for micropropagation.
The document outlines the key considerations for designing an effective plant tissue culture laboratory. It discusses that the laboratory should have separate areas for washing and storage, media preparation, aseptic transfer, and primary growth. The aseptic transfer area and primary growth room require stringent cleanliness, with HEPA filters, UV lights, and laminar flow hoods. Other areas require equipment for media preparation, sterilization, and storage. The primary growth room needs temperature, humidity, and lighting controls to suit the plants being cultured. Careful planning of traffic flow and separation of clean and dirty areas is important for success.
The title of this presentation is :-Establishment of animal tissue culture la...KESHAVPOSWAL
This presentation indicates that how to establish the animal tissue culture lab , what are the necessary conditions that should be taken into consideration while designing the animal tissue culture lab layout. It also emphasize on various instruments that generally used in any tissue culture lab.
Plant tissue culture is a trending and a promising area due to its applications. hence knowledge of its basics and history would be helpful for the pioneers. ans this presentation also discusses about the structure and establishment of a commercial tissue culture laboratory.
The document discusses the requirements and considerations for building and designing various sections of a clinical diagnostic laboratory. It covers architectural and engineering factors like lab module sizes, flexibility for expansion, and placement of utilities. It also describes the specific equipment, instruments, and facilities needed in different diagnostic areas like microbiology, parasitology, pathology, hematology, ELISA testing, PCR, and bacteriology sections. Proper ventilation, security, waste disposal, and health and safety measures are important across all laboratory areas.
Introduction and history, basic requirements forof PTC.pptxSujata Koundal
This document discusses plant tissue culture. It begins by defining plant tissue culture as the in vitro cultivation of plant cells or tissues under aseptic conditions. It then describes the basic requirements for a plant tissue culture laboratory, including equipment for media preparation, sterilization, culture incubation, and data collection. The document outlines general procedures for plant tissue culture, such as sterilizing glassware and explants, producing callus from explants, proliferating and sub-culturing callus, and creating suspension cultures. It provides details on surface sterilization methods and media used at different stages of culture.
Laboratory designed notes. For studyingElias910858
This document provides guidelines for designing laboratory spaces safely. It addresses considerations for general laboratory design including area layout, ergonomics, maintainability, infrastructure, and more. Specific guidance is provided for electrical systems, biosafety cabinets, ventilation, gas systems, waste handling, and other laboratory equipment and features. The document emphasizes compliance with codes and involving Environmental Health and Safety in the design process to ensure all laboratory needs and hazards are appropriately addressed.
The document lists and describes various laboratory equipment used for the mass production of biofertilizers, including autoclaves, hot air ovens, laminar air flow hoods, and other equipment. It provides details on the components, working principles, and uses of autoclaves, hot air ovens, and laminar air flow hoods, which are key pieces of equipment for sterilizing media, containers, and maintaining sterile environments for biofertilizer production.
A tissue culture lab requires specific equipment and facilities to successfully generate new plants through culturing cells and tissues. The lab needs separate rooms for transferring tissues, culturing under controlled conditions, refrigerating media and reagents, washing equipment, and transplanting new plants. Essential equipment includes glassware for sterilizing media, biosafety cabinets for sterile work, incubators for maintaining ideal growth conditions, centrifuges for media preparation, and microscopes for observations. Precise environmental controls and aseptic techniques are necessary throughout the process to produce healthy plants in the laboratory setting.
This document discusses the requirements and layout for an animal cloning laboratory. It provides answers to four questions:
1) The student chose animal cloning lab 502 because it is well-located and has proper ventilation and safety equipment.
2) A layout of the lab is provided including dimensions for tissue culture facilities, laminar flow hoods, incubation areas, and storage for sterile and non-sterile materials.
3) Future expansions could include replacing equipment with more advanced models, adding another laminar flow hood or sterile media preparation room.
4) Limitations for the lab include authorized entry only, double door entry, proper protective equipment, sterilization of the laminar flow hood, and proper ventilation
This document provides guidelines for maintaining proper biosafety and aseptic technique in a cell culture laboratory. It outlines considerations for laboratory layout and design such as maintaining asepsis, dust control, and limited traffic. Major areas of a cell culture lab include media preparation, wash-up, and sterilization areas. Key points for planning the layout include the number of users, available space, and location of preparation and storage areas. Guidelines are provided for handling cell lines and cultures, maintaining equipment, using proper labeling and protective equipment, and avoiding contamination.
Changing lifestyle and nuclear families are leading to exponential increase in the demand of ready to eat processed food in developing countries like India. A consumer would prefer traditional food on a daily basis, provided a safe, tasty and processed option is available. Therefore, this modern urban dynamic lifestyle leads to the genesis of discovery of Ready to Eat, Ready to serve and convenient food.
Retorting is one of the major technique used for the thermal processing of food products which are packed either in semi rigid flexible laminates or in metal or alloy cans. Retort Technology provides the advantage of processing the food and packaging together which makes the filled product more commercial stable. This technique is used for commercialization of traditional or ethnic dishes which are important due to their delicacy and are high in demand. It can be defined as the heating of low acid food prone to microbiological spoilage in hermetically sealed container to extend shelf
life.
Basic Facilities Required for Establishing Production Unit of Antagonistic Ba...Arunodaya Maji
Basic Facilities Required for Establishing Production Unit of Antagonistic Bacteria - There are many requirements to meet for establishing a proper production unit for mass production of biopesticides like antagonistic bacteria like pseudomonas.
Controlled atmosphere storage involves regulating oxygen, carbon dioxide, nitrogen, temperature and humidity levels to extend the shelf life of stored food products. Controlled atmosphere storage employs lower oxygen and higher carbon dioxide levels than ambient air. Controlled atmosphere storage inhibits bacterial and mould growth, protects against insects, reduces moisture loss and controls biochemical processes to slow ripening. Modern storage facilities include silos and bins of various materials and sizes. Controlled atmosphere storage can be combined with cold storage, which involves maintaining low temperatures to further extend shelf life. The design of a controlled atmosphere cold store involves considerations for insulation, refrigeration equipment, ventilation, storage chambers and maintenance procedures.
Similar to Dr. Yogesh Sumthane Assistant Professor College of Forestry Banda University of Agriculture and Technology (20)
This document provides an overview of wound healing, its functions, stages, mechanisms, factors affecting it, and complications.
A wound is a break in the integrity of the skin or tissues, which may be associated with disruption of the structure and function.
Healing is the body’s response to injury in an attempt to restore normal structure and functions.
Healing can occur in two ways: Regeneration and Repair
There are 4 phases of wound healing: hemostasis, inflammation, proliferation, and remodeling. This document also describes the mechanism of wound healing. Factors that affect healing include infection, uncontrolled diabetes, poor nutrition, age, anemia, the presence of foreign bodies, etc.
Complications of wound healing like infection, hyperpigmentation of scar, contractures, and keloid formation.
Beyond Degrees - Empowering the Workforce in the Context of Skills-First.pptxEduSkills OECD
Iván Bornacelly, Policy Analyst at the OECD Centre for Skills, OECD, presents at the webinar 'Tackling job market gaps with a skills-first approach' on 12 June 2024
The chapter Lifelines of National Economy in Class 10 Geography focuses on the various modes of transportation and communication that play a vital role in the economic development of a country. These lifelines are crucial for the movement of goods, services, and people, thereby connecting different regions and promoting economic activities.
This presentation was provided by Racquel Jemison, Ph.D., Christina MacLaughlin, Ph.D., and Paulomi Majumder. Ph.D., all of the American Chemical Society, for the second session of NISO's 2024 Training Series "DEIA in the Scholarly Landscape." Session Two: 'Expanding Pathways to Publishing Careers,' was held June 13, 2024.
Elevate Your Nonprofit's Online Presence_ A Guide to Effective SEO Strategies...TechSoup
Whether you're new to SEO or looking to refine your existing strategies, this webinar will provide you with actionable insights and practical tips to elevate your nonprofit's online presence.
Walmart Business+ and Spark Good for Nonprofits.pdfTechSoup
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Level 3 NCEA - NZ: A Nation In the Making 1872 - 1900 SML.pptHenry Hollis
The History of NZ 1870-1900.
Making of a Nation.
From the NZ Wars to Liberals,
Richard Seddon, George Grey,
Social Laboratory, New Zealand,
Confiscations, Kotahitanga, Kingitanga, Parliament, Suffrage, Repudiation, Economic Change, Agriculture, Gold Mining, Timber, Flax, Sheep, Dairying,
Dr. Yogesh Sumthane Assistant Professor College of Forestry Banda University of Agriculture and Technology
1. Current Avenues of Tissue Culture Laboratory
Development and Enhancement for Entrepreneurs
Speaker:- Dr. Yogesh Y. Sumthane
Assistant Professor
College of Forestry,BUAT, Banda.
2.
3. 3
Aim of this Training :-
The aim of this Tissue Culture (Training) in the subject for development of
skills for a successful career in an entrepreneurship and as well as to
generate technically trained human resource for Tissue Culture Industry.
4. Contents
1. Introduction
2. what is the current status of tissue culture
industry in India?
3. who set up the first tissue culture lab in India?
4. what are the general lab practices of tissue
culture lab?
5. Set Up (Place, Land, Building, Instruments, etc.)
6. Opportunities
5. What is the current status of tissue culture
industry in India?
At present, there are around 200 commercial tissue culture
companies in India with gross installed production capacity of about
500 million plantlets per annum.
The industry witnesses an actual production of approximately 350
million plants.
6. 6
"Business tissue culture was conceived in India in 1987 when AY. Thomas
and Organization Kerala (A VT) set up their first creation unit in Cochin
for clonal spread of prevalent genotypes of chose cardamom plants.
An ideal tissue culture laboratory should have at least two big rooms and a
small room. One big room is for general laboratory work such as
preparation of media, autoclaving, distillation of water etc. The other
big room is for keeping cultures under controlled light, temperature and
humidity.
Who set up the first tissue culture lab in India?
8. INTRODUCTION:
⦿ In designing any laboratory, big or small,certain elements
are essential for a successful operation.
⦿ The correct design of a laboratory will not only help
maintain asepsis, but it will also achieve a high standard of
work.
⦿ A convenient location is necessary for a
proper functional
laboratory.
9. STRATEGIC PLANNING:
⦿ Careful planning is required while remodeling old
structure or building a new laboratory facility.
⦿ The size of tissue culture lab and the amount and type of
equipment used depend upon the nature of the work to be
undertaken.
10. A standard tissue culture laboratory should provide
facilities for:
Washing and storage of glassware and plastic-ware
Preparation, sterilization and storage of nutrient media
Aseptic manipulation of plant material Maintenance of cultures
under controlled temperature, light and humidity
Observation of cultures, data collection and photographic
facility
Acclimatization of in vitro developed plants. The overall
design must focus on maintaining aseptic conditions.
13. LOCATION:
A good location includes the following:
1. Isolation from foot traffic.
2. No contamination from adjacent rooms.
3. Thermostatically controlled heat.
4. Water and drains for a sink.
5. Adequate electrical service.
6. Provisions for a fan and intake blower for ventilation.
7. Good lighting.
14. ⦿ Labs are frequently built as free-standing
buildings. Although more expensive to build, the
added isolation form adjacent activities will keep
the laboratory cleaner.
⦿ Prefabricated buildings make convenient lowcost
laboratories. They are readily available 9 in many
sizes in most parts of the country.
Built-in-place frame buildings can also be
used.
Consideration should be given to the following:
15. 1) Check with local authorities about zoning and building permits.
2) Locate the building away from sources of contamination such as a gravel driveway or parking lot,
soil mixing area, shipping dock, pesticide storage, or dust and chemicals from fields.
3) A clear span building allows for a flexible arrangement of walls.
4) The floor should be concrete or capable of carrying 50 pounds per square foot.
5) Walls and ceiling should be insulated to at least R-15 and be covered inside with a water-
resistant material.
6) Windows, if desired, may be placed wherever convenient in the media preparation and glassware
washing rooms.
7) The heating system should be capable of maintaining a room
temperature at 70 F in the coldest part of winter.
8) A minimum 3/4 in. water service is needed.
9) Connection to a septic system or sanitary sewer should be provided.
10) Air conditioning for summer cooling may be necessary.
11) Electric service capacity for equipment, lights and future expansion should be calculated.A
minimum 100 amp service is recommended.
16. GENERAL LABORATORY DESIGN
Laboratories should have easy to washwalls and floors.
Acrylic or urethane epoxy wall paints can be used; cement
floors can be painted with an epoxy or urethane floor
enamel or have an inlaid linoleum installed.
The traffic pattern and work flow in a laboratory must be
considered in order to maximize cleanliness.
17. The cleanest rooms or areas are the culture room, i.e.
primary growth room, and the aseptic transfer area. It is
best to design these rooms so they are not entered
directly from the outside of a building.
The media preparation area, glassware washing
area, or storage area should be located outside
these rooms.
The primary growth room and aseptic transfer room
should be enclosed with doors leading to each. Traffic
through these areas can be minimized by installing pass-
through windows
18. Ideally, the media preparation area wouldlead to the
sterilization area, which would lead to the aseptic
transfer room and eventually the primary growth room.
Unusual requirements for electricity and fire safety
dictate that power installation be done by professional
electricians. Most wiring will require 110 volts.
Temperature and fire 10 alarms are to be connected directly
to telephone lines to give fast warnings of problems. An
emergency generator should be available to operate
essential equipment during power outages.
19. GLASSWARE WASHING AND STORAGE AREA :
⦿ The glassware washing area should be located near the
sterilization and media preparation areas. When culture
vessels are removed from the growth area, they are often
autoclaved to kill contaminants or to soften semi-solid
media.
⦿ Locate the glassware storage area closeto the wash area
to expedite storage; these areas also need to be
accessible to the media preparation area.
20. The glassware area should be equipped withat least one
large sink; two sinks are preferable. Adequate work
space is required on both sides of the sink; this space will
be used for glassware soaking tubs and drainage trays.
Plastic netting can be placed on surfaces near the sink
to reduce glassware breakage and enhance water
drainage.
The pipes leading from the sink can be PVCto resist
damage from acids and alkalis.
21. Both hot and cold water should be available with water
distillation and/or deionization devices nearby.
Mobile drying racks can be stored nearby and lined with
cheesecloth to prevent water dripping and loss of small objects.
Locate ovens or hot air cabinets (75 C) close to the glassware
washing and storage area.
Dust-proof cabinets, low enough to allow easy access, can be
used in the storage area.
22. MEDIA PREPARATION AND STERILIZATION AREA
The water source and glassware storage area should be
convenient to the media preparation area.
Benches, suitable for comfortable working while standing (34
to 36in.) and deep enough (24 in.) to hold equipment listed
below are essential.
Their tops should be made with molded plastic laminate
surfaces that can tolerate frequent cleanings.
23.
24. ⦿ There is a variety of equipment available formicropropagation
laboratories; this equipment is generally located in the media
preparation area.
⦿ All laboratories need the following basics:
1. Refrigerator/freezer
2. High quality water
3. Balances
4. Hot plate/stirrer
5. pH meter
6. Aspirator or vacuum pump
7. Autoclave
25.
26. Optional equipment:
⦿ Microwave ovens are convenient for defrosting frozen
stocks and heating agar media.
⦿ Dissecting microscopes are useful to have in the laboratory for
meristeming, dissecting floral and shoot apices, and
observing plant culture growth.
⦿ Labwashers, or regular dishwashers, can be useful.
⦿ Automatic media dispensers are helpful when pipetting
large volumes of media.
27. PRIMARY GROWTH ROOM
⦿ Temperature, relative humidity, lighting units, and shelves need to be
considered in the culture room. All of these environmental considerations
will vary depending on the size of the growth room, its location, and the
type of plants grown within it.
⦿ Generally, temperatures are kept 76 +/-2 F.Heating can be accomplished by
traditional heating systems supplemented with heat from light ballasts or
space heaters. Cooling the room is usually a greater problem than heating;
cooler temperatures can be obtained by installing heat pumps, air
conditioners, or exhaust fans.
⦿ Using outside windows to cool culture rooms invites contamination problems
in the summer and humidity problems in the winter.
28. Some plant cultures can be kept in complete darkness; however, most
culture rooms are lighted at 1 klux (approximately 100ft-c) with some
going up to 5 to 10 klux. The plant species being micropropagated will
determine the intensity used.
Rooting has been shown to increase with far-red light; therefore, wide
spectrum lights should be used during stage III and cool-white lights can be
used during Stages I and II.
Automatic timers are needed to maintain desired photoperiods.
Reflectors can be placed over bulbs to direct their light.
Heat generated by the lights may cause condensation and temperature
problems.
In addition to using procedures previously mentioned, small fans with or
without polyethylene tubes attached, can be placed at the ends of shelves to
increase air flow and decrease heat accumulation.
29. Relative humidity (RH) is difficult to control inside growing vessels,
but fluctuations in the culture room may have a deleterious effect;
humidifiers can be used to correct this problem.
If the RH becomes too high, a dehumidifier is recommended.
Shelving within primary growth rooms can vary depending upon the
situation and the plants grown. Wood is recommended for
inexpensive, easy-to-build shelves.
The wood for shelves should be exterior particleboard or plywood
and should be painted white to reflect the room's light.
30.
31. ⦿ Expanded metal is more expensive than wood, but provides better
air circulation; wire mesh of 1/4 or 1/2 in.
⦿ Hardware cloth can be used but tends to sag under load.
⦿ Tempered glass is sometimes used for shelves to increase light
penetration, but it is more prone to breaking.
⦿ Air spaces, 2 to 4 in., between the lights and shelves will
decrease bottom heat on upper shelves and condensation in
culture vessels.
⦿ A room that is 8 ft high will accommodate 5 shelves, each 18 in.
apart, when the bottom shelf is 4 in. off the floor. The top and
bottom shelves may be difficult to work.
32. ASEPTIC TRANSFER AREA
▪ ⦿ The aseptic transfer area needs to be as clean as
possible.
▪ ⦿ It is preferable to have a separate room for aseptic
transfer; this decreases spore circulation and allows
personnel to leave shoes outside the room.
▪ ⦿ Special laboratory shoes and coats should be worn in
this area.
▪ ⦿ Laminar flow hoods or still-air boxes can b
e placed in this
room and used for all aseptic work.
33.
34. ⦿ Ultraviolet (UV) lights are sometimes installed in transfer areas to
disinfect the room; these lights should only be used when people
and plant material are not in the room.
⦿ Safety switches can be installed to shut off the UV lights when
regular room lights are turned on.
⦿ Surfaces inside the aseptic transfer area should be smooth to
minimize the amount of dust that settles.
⦿ Several electric outlets are to be installed to accommodate
balances, flow hoods, bacti- cinerators, and microscopes.
35. 35
How can you skyrocket the profits in your tissue
culture business?
1. Do proper market analysis
2. Improve Market Strategy
3. Networking and Communication
4. Follow up with the customers
5. Quality and effective delivery of Products
36. 36
Is tissue culture a Profitable Business?
1.In depth study of the National and International market
2.Proper estimation of costs to set up a lab
A. How to build your own tissue culture lab
B. The cost of setting up your own lab
3. Experience and Skill
38. 38
WHERE TO APPROACH FOR R&D FUNDS?
1. University Grants Commission (UGC)
2. All India Council for Technical Education (AICTE)
3. Council of Scientific and Industrial Research (CSIR)
4. Defence Research and Development Organisation (DRDO)
5. Department of Biotechnology (DBT)
6. Department of Science and Technology (DST)
7. Department of Scientific and Industrial Research (DSIR)
8. Ministry of Environment and Forests (MOEF)
9. NABARD
47. REFERENCES
⦿ Designing a Plant Micropropagation Laboratory Mark P. Bridgen
and John W. Bartok, Jr. Departments of Plant Science and
Agricultural Engineering University of Connecticut Storrs,
Connecticut 06268
Originally published in the Proceedings of the International Plant
Propagators Society, Vol. 37 (1987), pp. 462-467.
Reprinted here and distributed by permission of the senior author.
March 4, 1996. http://aggie-
horticulture.tamu.edu/tisscult/microprop/faciliti es/microlab.html