- Short term storage of semen involves storing semen at ambient or refrigerated temperatures for a few days using extenders. Long term storage involves cryopreservation of semen in liquid nitrogen. - Proper handling and storage of frozen semen involves keeping straws submerged in liquid nitrogen, regularly topping off liquid nitrogen, and minimizing temperature fluctuations during transport. - Transport of liquid semen involves using insulated containers with ice to keep temperatures below 10°C, while frozen semen requires special cryogenic containers and keeping the semen in liquid nitrogen during transport. - Proper maintenance of liquid nitrogen containers involves regularly checking liquid nitrogen levels, minimizing openings to reduce evaporation, and avoiding thermal and physical damage to