Pesticide residues in foods can pose risks if present at unsafe levels. This document discusses pesticide residues, including definitions and classifications of different pesticide types. It also covers factors that influence residue formation, such as application rate and environmental conditions. Risk assessment and monitoring programs set maximum residue limits and estimate exposure and intake to ensure pesticide residues do not exceed safe levels. International and national organizations monitor residues and set limits to protect public health.
Health hazards of agrochemical inputs in our foodJeebit Singh
This ppt deals with the health problems being faced due to various agro-chemical inputs being used in agriculture and its subsequent residue entering the human food chain
Pesticide residue analysis by simran.pptxSimran Bhatia
INTRODUCTION
The importance of food quality has become a serious issue due to the widespread use of pesticides. Though, the farmers have a conventional understanding of agriculture; they lack in the technical understanding of pesticides, their uses and safety aspects which makes them vulnerable (FAO, 2011).
Pesticide Residue: - The amount of insecticide left over after a lapse of time.” Disappearance of pesticide residue takes place by two ways i.e. Dissipation (In which the disappearance of the residue is fast) and Persistence (ability of pesticide to remain present for a long time).
Steps in Pesticide residue analysis:-
Sampling (collection, transport & storage)
Sample Preparation (Chopping/grinding/blending)
Extraction : Removal of pesticide residue from components that are present naturally
Clean-up : Removal of constituents that interfere with analysis of the pesticide residue of interest (Partitioning and purification )
Identification (detection), Quantification (determination) & Confirmation
4. EXTRACTION__________________________________________________________
Extraction is the process by which toxicant is transferred from the treated bulky biological material in to a solvent.
Principle : LIKE DISSOLVES LIKE and OPPOSITES DO NOT ATTRACT
QuEChERS_________________________________________________________________
Quick, Easy, Cheap, Effective, Rugged, and Safe.
◦ Developed in 2001 by American scientist Michelangelo
◦ Based on Acetonitrile extraction
◦ cleanup using dispersive-solid phase extraction (d-SPE) using MgSO4 and further purification with PSA and silica sorbent C18
◦ This method became popular because of its microscale extraction procedure which is simpler, consumes less time and organic solvent than all the previous methods.
Flow chart for QuEChERS sample preparation technique for pesticide residue analysis in FRUITS AND VEGETABLES
Status of Maximum Residue Limits in India:
The Central Insecticides Board and Registration Committee (CIBRC) registers pesticides in India and recommends them for various crops.
A total of 234 pesticides have been registered by CIBRC (including Endosulfan* the use of which was banned in India in May 2011).
Food Safety and Standard Authority of India (FSSAI) is responsible for setting MRLs for the pesticides that have been registered by CIBRC.
The MRLs for all registered pesticides should be set for all the crops they have been registered.
Pesticides have been linked to a number of health problems, including neurologic and endocrine (hormone) system disorders, birth defects, cancer, and other diseases.
Health hazards of agrochemical inputs in our foodJeebit Singh
This ppt deals with the health problems being faced due to various agro-chemical inputs being used in agriculture and its subsequent residue entering the human food chain
Pesticide residue analysis by simran.pptxSimran Bhatia
INTRODUCTION
The importance of food quality has become a serious issue due to the widespread use of pesticides. Though, the farmers have a conventional understanding of agriculture; they lack in the technical understanding of pesticides, their uses and safety aspects which makes them vulnerable (FAO, 2011).
Pesticide Residue: - The amount of insecticide left over after a lapse of time.” Disappearance of pesticide residue takes place by two ways i.e. Dissipation (In which the disappearance of the residue is fast) and Persistence (ability of pesticide to remain present for a long time).
Steps in Pesticide residue analysis:-
Sampling (collection, transport & storage)
Sample Preparation (Chopping/grinding/blending)
Extraction : Removal of pesticide residue from components that are present naturally
Clean-up : Removal of constituents that interfere with analysis of the pesticide residue of interest (Partitioning and purification )
Identification (detection), Quantification (determination) & Confirmation
4. EXTRACTION__________________________________________________________
Extraction is the process by which toxicant is transferred from the treated bulky biological material in to a solvent.
Principle : LIKE DISSOLVES LIKE and OPPOSITES DO NOT ATTRACT
QuEChERS_________________________________________________________________
Quick, Easy, Cheap, Effective, Rugged, and Safe.
◦ Developed in 2001 by American scientist Michelangelo
◦ Based on Acetonitrile extraction
◦ cleanup using dispersive-solid phase extraction (d-SPE) using MgSO4 and further purification with PSA and silica sorbent C18
◦ This method became popular because of its microscale extraction procedure which is simpler, consumes less time and organic solvent than all the previous methods.
Flow chart for QuEChERS sample preparation technique for pesticide residue analysis in FRUITS AND VEGETABLES
Status of Maximum Residue Limits in India:
The Central Insecticides Board and Registration Committee (CIBRC) registers pesticides in India and recommends them for various crops.
A total of 234 pesticides have been registered by CIBRC (including Endosulfan* the use of which was banned in India in May 2011).
Food Safety and Standard Authority of India (FSSAI) is responsible for setting MRLs for the pesticides that have been registered by CIBRC.
The MRLs for all registered pesticides should be set for all the crops they have been registered.
Pesticides have been linked to a number of health problems, including neurologic and endocrine (hormone) system disorders, birth defects, cancer, and other diseases.
Classification of insecticides based on chemical natureVinodkumar Patil
Classification of insecticides based on chemical nature, insecticides classified based on nature of inorganic insecticides, Organic insecticides, Synthetic organic insecticides, and Miscellaneous compounds
ppt of fate of pesticides in environment or environmental polution by pesticidesSundaresh Kalal
Nature is blessed with wide range of organisms where it perfectly balances the food chain and the ecosystem. Pseudomonas fluorescens, P. putida and some fungi antagonists are important biocontrol agents used against plant pathogenic bacteria which are known to produce special structures known as siderophores. Siderophores are defined as relatively low molecular weight, ferric ion specific chelating agents elaborated by bacteria and fungi growing under low iron stress (Neilands, 1981). The role of these compounds is to scavenge iron from the environment and to make the mineral. There are three main kinds of Siderophores known as hydroxamate, catecholate and carboxylate. Most organisms require iron as an essential element in a variety of metabolic and informational cellular pathways. In the aerobic environment iron exists mainly as Fe (III) and tends to form insoluble hydroxides and oxyhydroxides, making it largely unavailable to microorganisms. Therefore, they need mechanisms to solubilize Fe (III) to make it available for uptake; these mechanisms usually involve the production of siderophores.
A pesticide can be defined as any substance or mixture of substances intended for preventing, destroying, repelling, or mitigating any pest.
Pesticides like insecticides, herbicides, fungicides, and various other substances are used to control or inhibit plant diseases and insect pests.
The positive aspect of application of pesticides renders enhanced crop/food productivity and drastic reduction of vector-borne diseases.
However excessive use of these chemicals leads to the microbial imbalance, environmental pollution and health hazards.
Due to these problems, development of technologies that guarantee their elimination in a safe, efficient and economical way is important.
Classification of insecticides based on chemical natureVinodkumar Patil
Classification of insecticides based on chemical nature, insecticides classified based on nature of inorganic insecticides, Organic insecticides, Synthetic organic insecticides, and Miscellaneous compounds
ppt of fate of pesticides in environment or environmental polution by pesticidesSundaresh Kalal
Nature is blessed with wide range of organisms where it perfectly balances the food chain and the ecosystem. Pseudomonas fluorescens, P. putida and some fungi antagonists are important biocontrol agents used against plant pathogenic bacteria which are known to produce special structures known as siderophores. Siderophores are defined as relatively low molecular weight, ferric ion specific chelating agents elaborated by bacteria and fungi growing under low iron stress (Neilands, 1981). The role of these compounds is to scavenge iron from the environment and to make the mineral. There are three main kinds of Siderophores known as hydroxamate, catecholate and carboxylate. Most organisms require iron as an essential element in a variety of metabolic and informational cellular pathways. In the aerobic environment iron exists mainly as Fe (III) and tends to form insoluble hydroxides and oxyhydroxides, making it largely unavailable to microorganisms. Therefore, they need mechanisms to solubilize Fe (III) to make it available for uptake; these mechanisms usually involve the production of siderophores.
A pesticide can be defined as any substance or mixture of substances intended for preventing, destroying, repelling, or mitigating any pest.
Pesticides like insecticides, herbicides, fungicides, and various other substances are used to control or inhibit plant diseases and insect pests.
The positive aspect of application of pesticides renders enhanced crop/food productivity and drastic reduction of vector-borne diseases.
However excessive use of these chemicals leads to the microbial imbalance, environmental pollution and health hazards.
Due to these problems, development of technologies that guarantee their elimination in a safe, efficient and economical way is important.
Food Safety- WHO Theme 2015 calls for a new perspective in not just knowing the nutrient content of our food but also understanding how safe (or unsafe) it is! The ppt discusses some of the common chemicals in our food
TLC for chlorinated pesticide determination Bapi Mondal Bapi Mondal
In this assignment file i will demonstrate the process involved in Thin layer chromatography for Chlorinated Pesticide
determination. if u like this work feel free to share this file. thank you.
This lecture exposes students to food irradiation, the source of radiation, discusses whether it is save to consume irradiated foods and the effects of irradiation to food quality.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
2. Outline
• Introduction- Definitions
• Overview of different classes of pesticides
• Factors influencing the residue formation
• Risk Assessment & Monitoring Programmes on
Pesticide Residues in Food
3. Pesticide & Residues-
Definitions• Biocide by definition is any substance used with the intention
of killing living organisms whether these are pests or not.
• Pesticides are compounds that man uses to control, meaning
to reduce in number or to eradicate, organisms that interact
negatively with his activities such as crop production and
gardening, or to control disease in people, animals, etc.
• Pesticide Residues
Any substance or mixture of substances in food for man or
animals resulting from the use of a pesticide including any
specified derivatives, such as degradation and conversion
products, metabolites, reaction products and impurities
considered to be of toxicological significance.
4. Toxicity LabellingLabel Name Level of
toxicity
Oral lethal dose mg
per kg body weight of
test animal
Listed chemicals
Red label Extremely
toxic
1-50 Monocrotophos, zinc
phosphide, ethyl mercury acetate,
and others.
Yellow label Highly toxic 51-500 Endosulfan, carbaryl,quinalphos,
and others.
Blue label Moderately
toxic
501-5000 Malathion, thiram,glyphosate, and
others.
Green label Slightly toxic More than 5000 Mancozeb, oxyfluorfen, mosquito
repellant oils and liquids, and
most other household
insecticides.
Wikipedia
6. Organochlorine
• Organic compound containing at least one covalently
bonded atom of chlorine (the diphenylethanes, the
cyclodienes and the Cyclohexanes)- Highly Effective against
various Insects
• High persistence and Highly lipophilic
DDT
Dicolfol
Methoxychlor
X-Cl Y=H
X=Cl Y=OH
X=OCH3 Y=H
Aldrin Dialdrin
& Endrin
Endosulfan Lindane
7. Organophosphate
• acetylcholinesterase (AChE) in ganglia and in the parasympathetic
nervous system. [SLUD Syndrome]
• Highly Toxic , Less lipophilicity, Fast degradation
• Parathion (parathion-ethyl) introduced in 1944 -higher environmental
stability LD 50:3–13 mg kg−1BW
Common name Rat oral LD50 Rabbit dermal LD50
Chlorpyrifos 96–270 2,000
Diazinon 1,250 2,020
Dimethoate 235 400
Ethoprop 61.5 2.4
Fenamiphos 10.6–24.8 71.5–75.7
Malathion 5,500 >2,000
Methamidophos 13 (female only) 122
Methyl parathion 6 45
Ref,
Mello et al., 2003 : Food Safety:
Comtaminants and toxins
9. Pyrethroids
• Pyrethroids~ Pyrethrin (Tanacetum cinerariae-
folium)
Deltametrin-
LD50 for the fly
is 0.0003 µg
Common name Rat oral LD50 Rabbit dermal LD50
Allethrin 860 11,332
Bifenthrin 375 >2,000
Cyfluthrin 869–1271 >5,000 (rat)
Cyhalothrin 79 632 (rat)
Cypermethrin 250 >2,000
Deltamethrin 31–139 (female) >2,000
Esfenvalerate 451 2,500
Fenpropathrin 70.6–164 >2,000
Fluvalinate 261–282 >20,000
Permethrin 430–4,000 >2,000
10. Benzoylureas
• Synthesized between dichlobenil derivatives and
fenuron
• Act on the formation of chitin,hindering the
development of larvae during moult (by causing
the imperfect formationof the new cuticle) and
causing their death.
• They are not selective , affect CNS of mammals.
• Eg., Diflubenzuron, Flufenoxuron, Hexaflumuron
11. Fungicides
• Inorganic Fungicides-
Copper Salts
• Organic Fungicides in
Table
• DithiocarbomatesEthyl
ene thio urea
• Benzimidazoles- Systemic
Fungicide
• Dicarboximides- Resistant
• Triazoles - biosynthesis of
ergosterol
• Anilinopyrimidines- Act on
biosynthesis of AA
Ref,
Mello et al., 2003 : Food Safety:
Comtaminants and toxins
12. Herbicides
• mechanism of action of
herbicides is an
interaction with the
biochemical processes
of vegetables, they have
no toxicity for animals,
But
• Dermitis
• Paraquat- Toxic to lungs
• Percolate into soil
Ref,
Mello et al., 2003 : Food Safety:
Comtaminants and toxins
13. Formulation of Pesticides
WHY WE NEED
FORMULATION?
• Active Ingredient (AI)
shows activity at low doses
• Even Distribution
• Easy and safe distribution
TYPES OF FORMULATION
• Wettable powders
• Emulsifiable concentrates
• Granules
• Flowable powders
• Microcapsules
Points to remember:
• Dose Precision
• Contaminants
COMPOUNDS USED IN
FORMULATION:
• Adhesive Agent
• Anti Evaporative Agents
• Diluents for solids
• Dispersants
Penetrants
• Solvents
• Surfactants
Ref,
Mello et al., 2003 : Food Safety:
Comtaminants and toxins
14. Factors influencing in formation of
Residues in Food
• Disappearance rate
o Environmental Factors
o Crops
o Enzymatic degradation
o Washing
• Initial deposit
o Application rate
o Formulation
o Application techniques
o Influence of cultivar
o Shape of the cultivar
15. Initial Deposit
• Application rate(Per Hectare): 1st Generation –
1kg/, 2nd Generation – few Hundred Grams, Last
Generation- Few tens of grams
• Formulation: If granules gives progressive absorption
and a distribution effect
Cabras et.al., 1999
21. Residues in Processing of Foods:
• 1 l of wine is obtained from 1.5 kg of grapes,
• 1 l ofolive oil from 5 kg of olives
• 1 kg of dried prunes from 3 kg of plums,
22. Risk Assessment & Monitoring Programmes on
Pesticide Residues in Food
• Maximum Residue Limit (MRL)- maximum concentration of a
pesticide residue (expressed as mg/kg) that the CAC recommends be
legally permitted in food commodities and animal feeds
• MRLs recommended by the JMPR
TOXICOLOGICAL DOSSIER:
• ADI (Accetable Daily Intake) derived from
biochemical, metabolic, pharmacological, and toxicological properties
of the pesticide derived from studies of experimental animals and
observations in humans.
• no-observed-adverse-effect level (NOAEL)
ADI= NOAEL/Safety Factor (10 to 1000)
ENVIRONMENTAL DOSIER:
• predicting pesticide residue intake include the residue levels found in
practice, their distribution in the commodity, and the effect on
residues of the various processes used in the preparation of food
23. Exposure assessment:
Exposure to a pesticide residue present, or likely to be present under
GAP should be less than ADI
Risk characterization:
• Theoretical Maximum Daily Intake (TMDI) is used as a convenient screening
tool for assessing dietary intake
• the International Estimated Daily Intake (IEDI) is used to obtain a better
estimate of dietary exposure.
• TMDI and IEDI, the risk characterization is based on an average adult
weighing 60 kg
Ref,
Guidelines for predicting
dietary intake of pesticide
residues, WHO Publication
25. National Level
National Estimated Daily Intake differs from IEDI by
• Proportion of crop or commodity treated
• Proportions of crop or commodity produced domestically and imported
• Monitoring and surveillance data
• Total diet studies
Editor's Notes
these characteristicswere considered ideal for an insecticide ini-tially, they were soon found to be negativebecause of their persistence in the environ -ment,andtheirtendencytoaccumulateinthefood chain. Though not lethal, they directlyor indirectly affected the fertility and repro -ductionofmanywildspecies.Forthisreason,DDT and organochlorine compounds havebeen banned in agriculture since 1973 andheavily limited in the fight against thecarriers of diseases of mankind. Since themid-1980s, the use of DDT has been bannedin agriculture in all countries of the world.Aldrin, which degrades rapidly andforms its epoxide dieldrin by hydroxylation,is very stable in the environment. A half-lifeof 5 years in the soil has been calculated fordieldrin. Endrin is a stereoisomer of dieldrin.They are now used only in a very few specialcases such as the control of termites. Unlikethe other cyclodienes, endosulphan showsmoderate stability; in fruit and vegetables ittendstodegradeandformthecorrespondingsulphate with half-lives mostly rangingbetween 3 and 7 days.Hexachlorocyclohexane (HCH) mainlycontains four isomers ( α, β, γ and δ). Theisomer γ, lindane, which is the active isomer,has been isolated by crystallization from thisproduct.Lindaneistheleastpersistentamongthe organochlorine compounds.