This study analyzed virulence factors and phylogenetic grouping of Escherichia coli strains isolated from diabetic and non-diabetic patients with urinary tract infections in Tripoli, Libya. Using PCR, the study found that E. coli from diabetics contained fewer virulence genes and were more commonly in phylogenetic group A, while E. coli from non-diabetics contained more virulence genes and were more commonly in group D. E. coli resistant to ciprofloxacin also more commonly fell into group A. The study concludes that E. coli causing UTIs in diabetics are generally less virulent than in non-diabetics, and that virulence factors differ between the two host populations.
This mouse model has been build up and tested to evaluate anticancer agents in hepatocellular carcinoma. This transgenic model can also be tested for the testing of checkpoint inhibitors and other immunoactive agents.
Discovery of novel immunotherapy represents a main and intense focus of reseach in oncology. Proof of concept studies in animal represent a challenge and require a well characterized and appropriate animal models with most of the time customized approaches. Some recent developments and data generated for immune checkpoint modulators, adoptive cell transfer therapy, vaccines and bispecific T cell engagers will be presented.
This mouse model has been build up and tested to evaluate anticancer agents in hepatocellular carcinoma. This transgenic model can also be tested for the testing of checkpoint inhibitors and other immunoactive agents.
Discovery of novel immunotherapy represents a main and intense focus of reseach in oncology. Proof of concept studies in animal represent a challenge and require a well characterized and appropriate animal models with most of the time customized approaches. Some recent developments and data generated for immune checkpoint modulators, adoptive cell transfer therapy, vaccines and bispecific T cell engagers will be presented.
Effect of a specific combination of mannan oligosaccharides and β-glucans ext...Kawkab Abd El Aziz
Effect of a specific combination of mannan oligosaccharides and β-glucans extracted from yeast cell wall on the health status and growth performance of ochratoxicated broiler chickens
Predict the therapeutic effect of HDAC inhibitor panobinostat on leukemia by ...eilosei
Psanobinostat (LBH589) is a drug candidate in the process of Phase I to III clinical trials for various cancers including acute myeloid leukemia (AML) (DeWoskin and Million, 2013). It also showed anti-cancer effect in acute lymphoblastic leukemia (ALL) (Vilas-Zornoza A et al., 2012). The goal of our project is to predict the therapeutic effect of panobinostat on leukemia through the comparison of gene expression profiles in disease and drug treated cells. Our results predict that many disease signature gene expressions can be reversed upon panobinostat treatment. Moreover, we identified many leukemia-related oncogenes and tumor suppressor genes as the potential targets regulated by panobinostat.
Chair, David M. O'Malley, MD, Ana Oaknin, MD, PhD, and Matthew A. Powell, MD, prepared useful Practice Aids pertaining to endometrial cancer for this CME/MOC/AAPA activity titled “Endometrial Cancer Care in the Age of Immunotherapy: Translating Clinical Evidence Into Meaningful Improvements in Patient Outcomes Across the Disease Continuum.” For the full presentation, downloadable Practice Aids, and complete CME/MOC/AAPA information, and to apply for credit, please visit us at https://bit.ly/40bmalK. CME/MOC/AAPA credit will be available until July 3, 2024.
Overcoming the challenges of molecular diagnostics in government health insti...Yakubu Sunday Bot
overcoming the challenges of molecular diagnostics in government owned health institution in nigeria.Several challenges abound in the Nigerian health sector ranging from financial,political and lack of commitment.Its obvious and no wonder the state of health care deliveryy, vis a vis its quality of care to its citizenry.
Analyses of Risk Factors of Diarrhea in Patients with Esophagectomysemualkaira
Esophageal cancer is one of the most common
cancers of the world and surgery is an effective treatment for that.
However, long-term complications, such as diarrhea, are the focus
on the postoperative quality of life. Until now, the etiologies of
diarrhea after esophagectomy are still ill-defined.
Analyses of Risk Factors of Diarrhea in Patients with Esophagectomysemualkaira
Esophageal cancer is one of the most common cancers of the world and surgery is an effective treatment for that. However, long-term complications, such as diarrhea, are the focus on the postoperative quality of life. Until now, the etiologies of diarrhea after esophagectomy are still ill-defined.
Robert P. Edwards, MD, Chair of OB/GYN/RS, Co-Director of Women's Cancer Program at University of Pittsburgh, offers information about the current state of immunotherapy for recurrent ovarian cancer patients.
Effect of a specific combination of mannan oligosaccharides and β-glucans ext...Kawkab Abd El Aziz
Effect of a specific combination of mannan oligosaccharides and β-glucans extracted from yeast cell wall on the health status and growth performance of ochratoxicated broiler chickens
Predict the therapeutic effect of HDAC inhibitor panobinostat on leukemia by ...eilosei
Psanobinostat (LBH589) is a drug candidate in the process of Phase I to III clinical trials for various cancers including acute myeloid leukemia (AML) (DeWoskin and Million, 2013). It also showed anti-cancer effect in acute lymphoblastic leukemia (ALL) (Vilas-Zornoza A et al., 2012). The goal of our project is to predict the therapeutic effect of panobinostat on leukemia through the comparison of gene expression profiles in disease and drug treated cells. Our results predict that many disease signature gene expressions can be reversed upon panobinostat treatment. Moreover, we identified many leukemia-related oncogenes and tumor suppressor genes as the potential targets regulated by panobinostat.
Chair, David M. O'Malley, MD, Ana Oaknin, MD, PhD, and Matthew A. Powell, MD, prepared useful Practice Aids pertaining to endometrial cancer for this CME/MOC/AAPA activity titled “Endometrial Cancer Care in the Age of Immunotherapy: Translating Clinical Evidence Into Meaningful Improvements in Patient Outcomes Across the Disease Continuum.” For the full presentation, downloadable Practice Aids, and complete CME/MOC/AAPA information, and to apply for credit, please visit us at https://bit.ly/40bmalK. CME/MOC/AAPA credit will be available until July 3, 2024.
Overcoming the challenges of molecular diagnostics in government health insti...Yakubu Sunday Bot
overcoming the challenges of molecular diagnostics in government owned health institution in nigeria.Several challenges abound in the Nigerian health sector ranging from financial,political and lack of commitment.Its obvious and no wonder the state of health care deliveryy, vis a vis its quality of care to its citizenry.
Analyses of Risk Factors of Diarrhea in Patients with Esophagectomysemualkaira
Esophageal cancer is one of the most common
cancers of the world and surgery is an effective treatment for that.
However, long-term complications, such as diarrhea, are the focus
on the postoperative quality of life. Until now, the etiologies of
diarrhea after esophagectomy are still ill-defined.
Analyses of Risk Factors of Diarrhea in Patients with Esophagectomysemualkaira
Esophageal cancer is one of the most common cancers of the world and surgery is an effective treatment for that. However, long-term complications, such as diarrhea, are the focus on the postoperative quality of life. Until now, the etiologies of diarrhea after esophagectomy are still ill-defined.
Robert P. Edwards, MD, Chair of OB/GYN/RS, Co-Director of Women's Cancer Program at University of Pittsburgh, offers information about the current state of immunotherapy for recurrent ovarian cancer patients.
Jessica Maria Atrio, MD, has demonstrated that a safe but less-frequently prescribed birth control "mini-pill" is effective for women taking HIV medications. Study title: Effect of protease inhibitors on pharmacokinetics of oral norethindrone contraception in hiv+ women
Read the full story: http://sc-ctsi.org/index.php/news/sc-ctsi-supported-research-drives-global-health-policy#.Ut2b6WTTktU
Dr. Timothy Bowen (Cardiff University) - Data-driven systems medicinemntbs1
The summary of Dr. Timothy Bowen's presentation from the Jun 11-12th 2019 event Data-driven systems medicine at Cardiff University Brain Research Imaging Centre.
Connector Corner: Automate dynamic content and events by pushing a buttonDianaGray10
Here is something new! In our next Connector Corner webinar, we will demonstrate how you can use a single workflow to:
Create a campaign using Mailchimp with merge tags/fields
Send an interactive Slack channel message (using buttons)
Have the message received by managers and peers along with a test email for review
But there’s more:
In a second workflow supporting the same use case, you’ll see:
Your campaign sent to target colleagues for approval
If the “Approve” button is clicked, a Jira/Zendesk ticket is created for the marketing design team
But—if the “Reject” button is pushed, colleagues will be alerted via Slack message
Join us to learn more about this new, human-in-the-loop capability, brought to you by Integration Service connectors.
And...
Speakers:
Akshay Agnihotri, Product Manager
Charlie Greenberg, Host
Let's dive deeper into the world of ODC! Ricardo Alves (OutSystems) will join us to tell all about the new Data Fabric. After that, Sezen de Bruijn (OutSystems) will get into the details on how to best design a sturdy architecture within ODC.
Search and Society: Reimagining Information Access for Radical FuturesBhaskar Mitra
The field of Information retrieval (IR) is currently undergoing a transformative shift, at least partly due to the emerging applications of generative AI to information access. In this talk, we will deliberate on the sociotechnical implications of generative AI for information access. We will argue that there is both a critical necessity and an exciting opportunity for the IR community to re-center our research agendas on societal needs while dismantling the artificial separation between the work on fairness, accountability, transparency, and ethics in IR and the rest of IR research. Instead of adopting a reactionary strategy of trying to mitigate potential social harms from emerging technologies, the community should aim to proactively set the research agenda for the kinds of systems we should build inspired by diverse explicitly stated sociotechnical imaginaries. The sociotechnical imaginaries that underpin the design and development of information access technologies needs to be explicitly articulated, and we need to develop theories of change in context of these diverse perspectives. Our guiding future imaginaries must be informed by other academic fields, such as democratic theory and critical theory, and should be co-developed with social science scholars, legal scholars, civil rights and social justice activists, and artists, among others.
PHP Frameworks: I want to break free (IPC Berlin 2024)Ralf Eggert
In this presentation, we examine the challenges and limitations of relying too heavily on PHP frameworks in web development. We discuss the history of PHP and its frameworks to understand how this dependence has evolved. The focus will be on providing concrete tips and strategies to reduce reliance on these frameworks, based on real-world examples and practical considerations. The goal is to equip developers with the skills and knowledge to create more flexible and future-proof web applications. We'll explore the importance of maintaining autonomy in a rapidly changing tech landscape and how to make informed decisions in PHP development.
This talk is aimed at encouraging a more independent approach to using PHP frameworks, moving towards a more flexible and future-proof approach to PHP development.
UiPath Test Automation using UiPath Test Suite series, part 4DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 4. In this session, we will cover Test Manager overview along with SAP heatmap.
The UiPath Test Manager overview with SAP heatmap webinar offers a concise yet comprehensive exploration of the role of a Test Manager within SAP environments, coupled with the utilization of heatmaps for effective testing strategies.
Participants will gain insights into the responsibilities, challenges, and best practices associated with test management in SAP projects. Additionally, the webinar delves into the significance of heatmaps as a visual aid for identifying testing priorities, areas of risk, and resource allocation within SAP landscapes. Through this session, attendees can expect to enhance their understanding of test management principles while learning practical approaches to optimize testing processes in SAP environments using heatmap visualization techniques
What will you get from this session?
1. Insights into SAP testing best practices
2. Heatmap utilization for testing
3. Optimization of testing processes
4. Demo
Topics covered:
Execution from the test manager
Orchestrator execution result
Defect reporting
SAP heatmap example with demo
Speaker:
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
DevOps and Testing slides at DASA ConnectKari Kakkonen
My and Rik Marselis slides at 30.5.2024 DASA Connect conference. We discuss about what is testing, then what is agile testing and finally what is Testing in DevOps. Finally we had lovely workshop with the participants trying to find out different ways to think about quality and testing in different parts of the DevOps infinity loop.
Neuro-symbolic is not enough, we need neuro-*semantic*Frank van Harmelen
Neuro-symbolic (NeSy) AI is on the rise. However, simply machine learning on just any symbolic structure is not sufficient to really harvest the gains of NeSy. These will only be gained when the symbolic structures have an actual semantics. I give an operational definition of semantics as “predictable inference”.
All of this illustrated with link prediction over knowledge graphs, but the argument is general.
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...
PCR detection of virulence factors in Escherichia coli-Tripoli-Libya
1. PCR Detection of Virulence Factors in
Escherichia coli from
Diabetic and Non-Diabetic Patients
with Urinary Tract Infections
in Tripoli-Libya
Khalifa Sifaw Ghenghesh, Einass El-Kateb,
Nuri Berbash, Rania Abdel-Khalek,
Salwa F. Ahmed, John D. Klena
Faculty of Medicine, Tripoli University;
Faculty of Sciences; Tripoli University, Tripoli, Libya and
US Naval Medical Research Unit#3, Cairo, Egypt.
2. Introduction
• Uropathogenic Escherichia coli (UPEC) is the most
common cause of urinary tract infections (UTIs) in
humans.
• UPEC colonization of the UT is made possible by several
virulence factors (VF):
–
–
–
–
–
fimbrial adhesins >> e.g. type-1 fimbriae >> adherence
nonfimbrial adhesins >> adherence
sidrophores >> iron-acquisition systems
secreted toxins >> hemolysin
cytotoxic necrotizing factor 1 >> acts on epithelial or endothelial
cells, or monocyte-macrophages via
activation of GTP-binding proteins
– capsule-forming polysaccharides >> immune evasion
3. Introduction
• Phylogenetic groups of E. coli strains:
– Groups A and B1 >> commensal strains.
– Groups B2 and D >> mainly associated with E. coli
strains causing extraintestinal infections.
– Most VFs-containing UPECs cluster mainly either in
phylogeny group B2 or shared within groups B2 and
D
• Fluoroquinolones (e.g. ciprofloxacin):
– Drugs of choice in empirical treatment of
uncomplicated UTI.
– Studies have shown that resistance of UPEC to
ciprofloxacin and to may be associated with
decreased expression or loss of some virulence
factors
4. Introduction
• In developed countries:
– Several investigators have reported that infections
including UTIs are more frequently reported among
diabetic patients than the general population, while
others found no such difference.
– Studies have shown that hosts with predisposing
factors, such as diabetes, can acquire UTIs caused
by less virulent E. coli strains.
• In developing countries.
– Lack of information on the phylogenetic grouping
and virulence factors of UPEC from diabetic and
non-diabetic patients.
5. Objectives of the Study
• To determine the phylogenetic grouping and
the virulence factors of UPEC isolated from
diabetic and non-diabetic patients in Tripoli,
Libya using PCR techniques.
6. Methods
• Using multiplex PCR
– 48 E. coli isolates from Libyan patients with UTIs:
• 19 from diabetics and 29 from non-diabetics
• Examined for the presence of genetic determinants of
19 extraintestinal virulence factors.
• The primer pairs for the 19 virulence genes and
amplification conditions have been described
previously (Johnson & Stell 2000).
• Examined for phylogenetic grouping (A, B1, B2, or D) as
previously reported (Clermont et al., 2000).
• Reactions were performed in a GeneAmp PCR System
9700 (Applied Biosystems, Foster City, CA, USA).
• DNA sequencing was used to confirm the identity of
amplified PCR products and establish positive controls.
8. Results
• Of the virulence genes examined, 3 or more
were found in 37.5% of E. coli studied.
• Only genes fimH (coding for type 1 fimbriae)
and traT (coding for serum resistance) were
both found significantly more common in E.
coli from non-diabetic than in diabetic patients
(P<0.02, OR=7.88 and P<0.00007, OR=14.38,
respectively).
• several of the VFs detected in the present study
were not reported previously in E. coli isolates
from diabetics with UTI and these include fyuA,
kpsMT II, and traT.
11. Phylogeny groups among UPEC isolated from diabetics and
non-diabetics and in relation to ciprofloxacin resistance
Non
Phlyogeny
Diabetics
diabetics
Ciprosusceptible
Ciproresistant
group
(n=19)
(n=29)
(n=38)
(n=10)
---------------------------------------------------------------------------------------------------------------------
A
B1
B2
D
14 (74)1
0 (0.0)
3 (16)
2 (11)
4 (14)
1 (3.4)
8 (28)
16 (55)3
10 (26)
1 (3)
10 (26)
17 (45)4
8 (80)2
0 (0.0)
1 (10)
1 (10)
12. Results
• E. coli from diabetics is significantly statistically
associated with phylogeny group A than E. coli from
non-diabetics (P<0.00003, OR=17.50).
• E. coli from non-diabetics is significantly statistically
associated with phylogeny group D than E. coli from
diabetics (P<0.002, OR=10.46).
• Ciprofloxacin-resistant E. coli is significantly
statistically associated with phylogeny group A than
ciprofloxacin-susceptible E. coli (P<0.002, OR=11.20).
• Ciprofloxacin-susceptible E. coli is significantly
statistically associated with phylogeny group D than
ciprofloxacin-resistant E. coli (P<0.05, OR=7.29).
13. Conclusion
• In developing countries, UPEC causing UTIs in
diabetics are less virulent than UPEC causing UTIs
in non-diabetics which confirms the findings of
previously published studies from developed
countries.
• Studies are needed on the prevalence of VFs and
phylogenetic groups in UPEC from diabetic patients
in developing areas.
• These studies are important in understanding the
roles of these factors in causing UTI in diabetics as
well as in non-diabetics, which in turn may lead to
development of universal vaccines to prevent such
infections.