PCR (Polymerase Chain Reaction)

2.  PCR - stands for Polymerase Chain Reaction.
 Nicknamed as " "
 First developed by in 1983.
 Major Impact on many areas of Molecular Cloning and Genetics.
 A target sequence of DNA can be amplified a billion fold in several hours.
 Procedure-carried out entirely invitro.
 Carried out in
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 Target sequence of DNA is heated to denature the target DNA into
single strands that can act as a template for DNA synthesis

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 are short, single-stranded sequences of nucleic acid
(i.e., oligonucleotides usually 20 to 30 nucleotides long)
 The DNA is then cooled to allow the primers to anneal,that
is,bind the appropriate complementary strand.
Complementary sequence to C-A-T-G ...Can anyone try??
G-T-A-C

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NOTE :-
Taq Polymerase-
📌 Thermostable DNA
Polymerase
📌 Obtained from
Thermus aquaticus
📌 Bacterial species
📌 Grows in hot
springs(90degree
Celsius)
📌 Not denatured by
any heating steps

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 In the presence of Mg2+,DNA polymerase extends the
primers on both the template strands from 5' to 3' by its
polymerase activity.
 Primer extension is performed at a temperature for the
particular polymerase that is used.

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 The cycle of heating,cooling,and replication repeated 25 or 30
times over a few hours,the segment of DNA can be amplified
to approximately billion times i.e., 1 billion copies are made.
 The amplified fragment if desired can now be used to ligate
with vector for further cloning.

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