DNA profiling was used to solve a simulated crime in a laboratory experiment. The goal was to match DNA samples from a crime scene to suspects using PCR to amplify DNA segments and electrophoresis gel to visualize DNA fragments. Analysis of the gel showed the crime scene DNA matched the DNA of suspect three, identifying them as responsible for the crime.

1. University of Puerto Rico<br />Cayey Campus<br />Jessica Díaz Rivera<br />Prof. Elena Gonzalez<br />Laboratory #5: DNA Profiling<br /> The laboratory that Dr. Belinda Roman gave was about DNA Profiling of how to solve a crime or medical problem. The objective of the experiment was to solve a crime, using DNA Profiling otherwise known as Forensic DNA Fingerprinting. DNA Profiling is a technique used by scientists to distinguish between individuals of the same species using only samples of their DNA. It is used to solve crime or medical problems. The DNA profile of each individual is highly specific. We did a PCR (Polymerase Chain Reaction) to amplify specific segments of DNA and electrophoresis gel to see DNA fragments. We had the crime scene samples as a positive control and the samples of suspects one to five. In the agarose gel electrophoresis we analyzed the different bands of DNA and compared them to the crime scene DNA. At the end, we concluded that the DNA from the crime scene was similar to the DNA of the third suspect. Therefore suspect number three was responsible for the crime. <br />