DNA fingerprinting

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DNA fingerprinting

  1. 1. February 25, 2011 DNA Fingerprinting:This lab was meant to help us identify a few samples of DNA retrieved from a crimescene. We had 5 samples from suspects and one from the crime scene. The first partconsists of cutting the DNA at specific sites using a certain restriction enzyme thatspecifically binds to the desired segment to cut. The second part consists of the actualelectrophoresis of the samples. Here the DNA will displace itself according to the size ofthe molecules in each well. Since the restriction enzyme cuts at certain segments, thenit should cut the criminals DNA in the same place it would on any other molecule. Thedifference is that when the DNA is cut, the variation among humans will create differentlengths of DNA meaning that, if the criminal’s DNA is cut by the same enzyme 20 times,then you will get the same results all 20 times. After electrophoresis, the DNA thatmoved to the exact same spot that the crime scene sample moved to was suspect 3.This means that this suspect’s DNA is the same to the DNA sample found in the crimescene.

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