This study evaluated the anti-acne potential of essential oils (EOs) from seven Mediterranean plants against acne-causing bacteria. Oregano EO showed the strongest antimicrobial activity against Propionibacterium acnes and Staphylococcus epidermidis based on disc diffusion, MIC, MBC, and MBIC assays. Oregano oil was formulated into a nanoemulsion and demonstrated superior healing and antimicrobial effects compared to a reference antibiotic in a mouse model of acne. The results suggest oregano oil nanoemulsion is a potential natural alternative for treating acne and combating emerging antibiotic resistance.

1. UNIVERSITY INSTITUTE OF PHARMACY
CSJM UNIVERSITY,KANPUR
Origanum vulgare L. Essential Oil as a Potential Anti-Acne
Topical Nanoemulsion—In Vitro and In Vivo Study.
Authors: Mohammed H. Taleb, Nourtan F. Abdeltawab , Rehab N. Shamma , Sherein
S.Abdelgayed , Sarah S. Mohamed , Mohamed A. Farag and Mohammed A. Ramadan.
Journal: Molecules (Impact factor: 3.2)
Supervisor
Dr. Nisha Sharma
Associate professor
University Institute of
Pharmacy, C.S.J.M. University,
Kanpur.
Presented By-
Ruchi pal
Roll no.-9561008
M.pharm.(Pharmaceutics)
3rd Sem.2019-20 Batch 1

2. CONTENTS
Introduction
Objective of
study
Methodology
Results
Conclusion
References
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3. INTRODUCTION
• Acne vulgaris is the 8th most prevalent disease and
second top skin disease globally.
• It results in the formation of non-inflammatory
comedones and inflammatory lesions such as
papules, pustules, and nodules.
• Two bacteria are associated with acne
pathogenesis: Propionibacterium acnes and
Staphylococcus epidermidis.
• Anti-acne therapy includes systemic and topical
therapies.
• Topical therapies include comedolytic agents, anti-
inflammatory agents and antibiotics .
• Bacterial resistance accompanies topical anti-acne
antibiotics.
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4. •Resistance to topical anti-acne antibiotics is attributed to multiple factors,
including use of repeated single antibiotic, sub-inhibitory concentrations, or
use over extended time .
•To overcome the emerging resistance to conventional antibiotics, alternative
natural antimicrobial agents have been investigated.
•Essential oils (EOs) of aromatic plants such as oregano, tea tree oil,
lemongrass, and thyme have antimicrobial activities that can be used as a
natural alternative.
•Therefore, this study tested the anti-acne potential of seven EOs
used in Mediterranean folk medicine: Oregano (Origanum vulgare),
thyme (Thymus vulgaris), lemongrass (Cymbopogon citratus), tea
tree (alternifolia), mentha (Mentha piperita), lavender (Lavendula
anguestifolia), and chamomile (Matricaria recutita).
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5. Objective of study
To assessed the antimicrobial
activity of the selected EOs
against acne-causing bacteria
in-vitro.
To develop a pharmaceutical
formulation of the EO with the
highest antimicrobial effect.
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6. Methodology
Bacterial strains and
culturing
Screening of
Antibacterial Activities
of Eos by Disc
Diffusion Method
Determination of the
MIC and MBC of the
EOs
Minimum Biofilm
Inhibitory
Concentration(MBIC)
Determination of Kill
Kinetics
Gas Chromatography-
Mass Spectroscopy
Development of
Nanoemulsion
In-Vivo Antiacne
Experiment
Statistical Analysis
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7. RESULTS
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8. Screening of Antibacterial Activity of Tested EOs Using Agar
Disc Diffusion Method
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9. Antimicrobial Activity of the Tested EOs Using
Minimal Inhibitory Concentration (MIC) Method
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10. The Bactericidal and Anti-Biofilm Activities
of the Tested EOs
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11. Figure 2. Oregano EO inhibited the growth of S. epidermidis faster than P. acnes.
Oregano EO at concentrations of 0.672, 1.34, 2.68 mg/mL (1, 2, 4 MIC) and no EO
control was used for assaying the killing rate of bacterial cells at 0, 2, 4, 8 and 12 h and
expressed as the surviving bacteria (log10 CFU/mL) at different exposure times. We
used starting bacterial suspension concentration 10 8 CFU/mL. Oregano EO killing rate
against (a) S. epidermidis and (b) P.acnes.
Killing Dynamics of Oregano EO against
S. epidermidis and P. acnes
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12. Chemical Composition of Oregano and Thyme
EOs
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13. Antimicrobial Activity of Thymol Using In Vitro Disc
Diffusion, MIC, MBC and MBIC Assays
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14. 14

15. Assessment of In Vivo Antimicrobial and Healing Activities of Oregano
EO Nanoemulsion Using In Vivo Acne Mouse Model
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16. 16

17. 17

18. Conclusion
Oregano EO exhibited the strongest antimicrobial and
antibiofilm effect against the tested acne-causing
bacteria.
Both tested acne-causing bacteria were resistant to
chamomile, lavender, and mentha EOs.
Oregano oil’s nanoemulsion showed superior healing
and antimicrobial effects compared to the reference
antibiotic.
Oregano oil nanoemulsion is a potential natural and
effective alternative for treating acne and overcoming
the emerging antibiotic resistance. 18

19.  Oregano, the most effective EO, was formulated and tested as a nanoemulsion in
an acne animal mouse model. The formulation showed superior healing and
antimicrobial effects compared to the reference antibiotic. Collectively, this data
suggested that oregano oil nanoemulsion is a potential natural and effective
alternative for treating acne and overcoming the emerging antibiotic resistance.
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20. References
• Nishijima, S.; Kurokawa, I.; Katoh, N.; Watanabe, K. The bacteriology
of acne vulgaris and antimicrobial susceptibility of Propionibacterium
acnes and Staphylococcus epidermidis isolated from acne lesions. J.
Dermatol. 2000, 27, 318–323.
• Kinney, M.A.; Yentzer, B.A.; Fleischer, A.B., Jr.; Feldman, S.R. Trends in
the treatment of acne vulgaris: Are measures being taken to avoid
antimicrobial resistance? J. Drugs Dermatol. JDD 2010, 9, 519–524.
• Gavaric, N.; Mozina, S.S.; Kladar, N.; Bozin, B. Chemical profile,
antioxidant and antibacterial activity of thyme and oregano essential
oils, thymol and carvacrol and their possible synergism. J. Essent. Oil
Bear. Plants 2015, 18, 1013–1021.
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21. THANK YOU
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