A one-to-one function is a function where each input value is mapped to a unique output value, so that no two inputs map to the same output. A function is one-to-one if any two different inputs x1 and x2 will always produce different outputs f(x1) and f(x2). The document provides examples of relations that are and are not one-to-one functions, such as the relation between airports and their unique three-letter codes being a one-to-one function, while the relation between distance traveled on a jeepney route and fare is not one-to-one as different distances can have the same fare.