Creative Biolabs is glad to show a series of unique de novo antibody sequencing services for research, diagnostic, and therapeutic industries. To overcome the current drawbacks of sequencing based on traditional methods, Creative Biolabs has developed the proprietary Database Assisted Shotgun Sequencing (DASS) technology, which based on our next generation antibody sequencing platform. Our services consist of variable region, variable plus leader region, as well as full-length heavy- and light-chain antibody sequencing for all species, isotypes, and allotypes. Purified monoclonal antibodies in multivalent forms can be sequenced with 100% coverage of the desired regions as well as excellent accuracy. Numerous successful cases from Creative Biolabs have confirmed our qualification to provide antibody sequencing with 100% accuracy and satisfaction guarantee to meet our customers’ needs.

1. next generation antibody sequencing
• Creative Biolabs is glad to show a series of unique de novo antibody
sequencing services for research, diagnostic, and therapeutic industries. To
overcome the current drawbacks of sequencing based on traditional methods,
Creative Biolabs has developed the proprietary Database Assisted Shotgun
Sequencing (DASS) technology, which based on our next generation antibody
sequencing platform. Our services consist of variable region, variable plus
leader region, as well as full-length heavy- and light-chain antibody
sequencing for all species, isotypes, and allotypes. Purified monoclonal
antibodies in multivalent forms can be sequenced with 100% coverage of the
desired regions as well as excellent accuracy. Numerous successful cases from
Creative Biolabs have confirmed our qualification to provide antibody
sequencing with 100% accuracy and satisfaction guarantee to meet our
customers’ needs.

2. • Our world-leading DASS system provides reliable supporting proof for
• Confirmation & validation of produced mAb
• QC analysis
• IND/NDA application

3. • Instrumentation
• We could use a high-field Orbitrap Elite hybrid MS (Thermo Scientific) with 240.000 resolution and <1 ppm mass accuracy to perform our services. The available fragmentation
techniques will include CID, HCD, and ETD. The combined usage of these technologies could ensure the maximum coverage to generate high-quality results for our clients.
• Sequence Validation Guarantee
• As we are aware that your projects have tight timelines we validate the sequences to ensure that your antibody will be functional with the first attempt.
• (1) The masses of the reduced light- and heavy-chain will be determined to validate the determined sequences. The light chain sequence must match within a +/- 1.2 Da range.
For the heavy chain, +/- 1.8 Da is allowed. If the determined sequences do not match with the protein mass, you will not be charged. (Please note that this deviation is less than
the weight of two protons.)
• (2) Each mutation in the antibody will be validated by at least two meaningful spectra. This ensures that your sequence does not contain isobaric exchanges. Do not rely on
peptide maps alone. [Isobaric exchanges are exchanges of identical mass like GG=N, YM=FM (Ox). The peptide maps used by some competitors are often not sensitive to this kind
of error.]