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MUSHROOM CULTURE - PREPARATION OF MEDIUM
Edible mushrooms are the fleshy and edible fruit bodies of several
species of macrofungi . They can appear either below ground
(hypogeous) or or above ground (epigeous)
1. White Button Mushroom (Agaricus bisporus) ‘; This mushroom
may be known as common mushroom, button mushroom, white
mushroom, cultivated mushroom, table mushroom, and
champignon mushroom.
2. Oyster Mushroom ((Pleurotus ostreatus)
3. Volvariella volvacea (paddy straw mushroom or straw
mushroom)
4. Shiitake (Lentinula edodes)
5. Chanterelle (Cantharellus cibaris, C. formosus, etc)
6. Enokitake (Flammulina velutipes) etc
MUSHROOM CULTURE - PREPARATION OF MEDIUM
Preservation and storage of culture
Proper maintenance of pure cultures of cultivated mushroom is
necessary to maintain vigour and productivity. There is no
satisfactory way to check and evaluate the qualities of spawn by any
rapid on the spot examination. The strains of cultivated mushroom
must be suitably preserved and carefully tested from time to time
for vigour and productivity.
Conventional methods of culture preservation
Periodic transferStock cultures are maintained by periodic transfer
on a suitable solid substrate or natural/semi -synthetic agar media.
Isolation Pure cultures are raised by using single or mass
basidiospore isolation or tissue culture technique from freshly
harvested fruit bodies.

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must.pptx

  • 1. MUSHROOM CULTURE - PREPARATION OF MEDIUM Edible mushrooms are the fleshy and edible fruit bodies of several species of macrofungi . They can appear either below ground (hypogeous) or or above ground (epigeous) 1. White Button Mushroom (Agaricus bisporus) ‘; This mushroom may be known as common mushroom, button mushroom, white mushroom, cultivated mushroom, table mushroom, and champignon mushroom. 2. Oyster Mushroom ((Pleurotus ostreatus) 3. Volvariella volvacea (paddy straw mushroom or straw mushroom) 4. Shiitake (Lentinula edodes) 5. Chanterelle (Cantharellus cibaris, C. formosus, etc) 6. Enokitake (Flammulina velutipes) etc
  • 2. MUSHROOM CULTURE - PREPARATION OF MEDIUM
  • 3.
  • 4. Preservation and storage of culture Proper maintenance of pure cultures of cultivated mushroom is necessary to maintain vigour and productivity. There is no satisfactory way to check and evaluate the qualities of spawn by any rapid on the spot examination. The strains of cultivated mushroom must be suitably preserved and carefully tested from time to time for vigour and productivity. Conventional methods of culture preservation Periodic transferStock cultures are maintained by periodic transfer on a suitable solid substrate or natural/semi -synthetic agar media. Isolation Pure cultures are raised by using single or mass basidiospore isolation or tissue culture technique from freshly harvested fruit bodies.