This presentation is fetures the basic introduction to Genome mosaicism in humans and nature, with some examples of its harmful effects on humans, with
In this presentation, I talk about the various forms of chimerism observed in humans like tetragametic chimerism, twin chimerism and fetomaternal microchimerism and some case studies related to chimerism.
Karyotyping is the process by which photographs of chromosomes are taken in order to determine the chromosome complement of an individual, including the number of chromosomes and any abnormalities.
The term is also used for the complete set of chromosomes in a species or in an individual organism and for a test that detects this complement or measures the number.
A cytological technique to detect the nature of adjacent chromosomal regions by using different staining technique assisted with some pre treatment of metaphase chromosomes prepared on the slides
This presentation is fetures the basic introduction to Genome mosaicism in humans and nature, with some examples of its harmful effects on humans, with
In this presentation, I talk about the various forms of chimerism observed in humans like tetragametic chimerism, twin chimerism and fetomaternal microchimerism and some case studies related to chimerism.
Karyotyping is the process by which photographs of chromosomes are taken in order to determine the chromosome complement of an individual, including the number of chromosomes and any abnormalities.
The term is also used for the complete set of chromosomes in a species or in an individual organism and for a test that detects this complement or measures the number.
A cytological technique to detect the nature of adjacent chromosomal regions by using different staining technique assisted with some pre treatment of metaphase chromosomes prepared on the slides
Mitochondrial Inheritance
Apart from the nucleus DNA is also found within another cellular organelle, the mitochondrion.
Each mitochondrion contains multiple copies of a double-stranded, circular DNA molecule of 16,569 base pairs.
This DNA has 37 genes out of which encodes 13 peptides that are subunits of proteins required for oxidative phosphorylation.
There is a complete set of 22 transfer RNAs and two ribosomal RNAs.
Mitochondrial Inheritance
Apart from the nucleus DNA is also found within another cellular organelle, the mitochondrion.
Each mitochondrion contains multiple copies of a double-stranded, circular DNA molecule of 16,569 base pairs.
This DNA has 37 genes out of which encodes 13 peptides that are subunits of proteins required for oxidative phosphorylation.
There is a complete set of 22 transfer RNAs and two ribosomal RNAs.
Introduction, Types-somatic and germinal; Mechanism of meiotic crossing oversynapsis, duplication of chromosomes, breakage and union, terminalization;
Cytological basis of crossing over - Stern’s experiment in Drosophila; Creighton
and McClintock’s experiment in Maize; Crossing over in Drosophila, Construction
of genetic maps in Drosophila - two point and three-point crosses; Interference and
coincidence.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
3. MOSAICISM AND CHIMERISM
• Mosaicism and chimerism refers to one organism
with two or more distinct populations of cells.
• But they are two different concepts.
• Because mosaics start with the same genome, but
chimeras is a fusion of two different genomes.
• Mosaicism and chimerism differ in the mechanisms
by which each is prevented
4. MOSAICISM
• Mosaicism is a condition in which presence of two or more
chromosomal complements found in the same tissue of an
individual where cells derived from the same genetic
origin/same zygotic genotype.
• This can occur in any type of cells.
5. • Mosaicism can arise when there is a mutation in early
development so there can be group of cells that
behave differently.
• This can happen on a gene level or even whole
chromosomes.
• For example, a girl has a mixture of 45X and 46XY
cells in her body. One cell early in development
dropped the Y chromosome and the majority of her
cells are 45X.
• Pattern of inheritance: Both autosomal dominant
and recessive
8. Types of mosaicism
• Germinal mosaicism
• Somatic mosaicism
• Germinal and somatic mosaicism
Germinal mosaicism:
Asymptomatic,
only germinal cells are affected,
could be transmitted to progeny
E.g.: DMD, Turner syndrome, Hemophilia
Somatic mosaicism:
Symptomatic
Somatic cells are affected
It is not inherited
10. • E.g: cancer, down syndrome
Germinal and somatic mosaicism: presence of both somatic
and germline mosaic.
Diagnosis: FISH, Karyotype
Impact of mosaic on disease: depends on stage of mutation,
nature of chromosomal abnormality, proportion between mosaic
and normal cells, nature of affected cells.
Disease:
Heteroplasmy: somatic mosaicism results in random segregation
and propagation of mutant mitochondria.
Cancer: several recent studies shows that probability of cancer
progression depends on the degree of mosaicism.
11. CHIMERISM
• Chimerism is a condition in which presence of two or more
chromosomal complements found in the same tissue of an
individual where cell line not derived from a single zygote.
• Chimeras can happen with organ transplantation (because the
donor cells are different from recipient), twin (a pregnancy
may start out with two or more zygotes but not all survive and
are then absorbed by remaining fetus).
• In chimerism the divergent genotypes are usually found in all
across the genome. Chimerism leads to the dilemma in sex
determination.
12. Types of chimerism
• Artificial chimerism: via blood transfusion, organ, stem cell
and bone marrow transplantation.
• Twin/ multiple gestation: via trans placental passage of
second cell line.
• In naturally occurring chimerism XX/XY is highly frequent.
It is increases with increase in IVF birth
• Tetragametic: via fusion of two fertilized zygote.
13. Types of natural chimera:
• Fetal- maternal chimera
• Blood sharing and twin chimera
• Whole body or dispermic chimera
• Tumor chimera
• Germ cell chimera
14. • Test to done before transplantation are, HLA, red
cells phenotype studies, peripheral blood and skin
fibroblast karyotyping, FISH study.
15. References
• Mosaicism and chimerism as components of intraorganismal
genetic heterogeneity, B.SANTELICES, J . EVOL. B IOL. 17
(2004 ) 1187–1188
• Mechanisms of mosaicism, chimerism and uniparental disomy
identified by single nucleotide polymorphism array analysis
Laura K. Conlin, Brian D. Thiel, Carsten G. Bonnemann,
Livija Medne, Linda M. Ernst, Elaine H. Zackai, Matthew A.
Deardorff, Ian D. Krantz, Hakon Hakonarson and Nancy B.
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