Monkeypox is a zoonotic disease endemic in the Democratic Republic of Congo (DRC) but prevalent also in other countries of Central and Western Africa. The clinical presentation of monkeypox closely resembles the one of smallpox. The mortality rate is officially about 11% however rates as high as 17% have been observed. The disease has been considered rare and not much attention is paid to it. Nonetheless, the incidence of monkeypox increased 20-fold from 1981-1986 to 2005-2007 (two active surveillance programs). More research, surveillance and effective interventions are needed to ensure it would not gain the potential to become the next global pandemic.
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Monkeypox update
1. Epidemiology and
laboratory diagnosis of
Monkeypox virus in Africa
Nikola Sklenovská
Master of Biomedical Sciences
KU Leuven
Supervisor: prof. dr. Marc VAN RANST
Co-supervisor: dr. Piet MAES
Instructor: Valentijn VERGOTE
9. 9
1. Assess epidemiological characteristics of the
most recent monkeypox data.
2. Develop a diagnostic assay for differentiation of
MPXV and VZV suitable for field conditions.
Aims
10. 10
Democratic Republic of Congo
• Provided by prof. dr. Muyembe
• September 2014 – February 2016
• Passive surveillance
• 223 laboratory confirmed monkeypox cases
Materials and Methods
19. 19
1. Assess epidemiological characteristics of the
most recent monkeypox data.
2. Develop a diagnostic assay for differentiation of
MPXV and VZV suitable for field conditions.
Aims
27. 27
Conclusions
• Monkeypox incidence is increasing need for effective
interventions, surveillance and research
• Multiplex qPCR diagnostic assay suitable for field
conditions was developed
• VZV and MPX coinfections are not rare events?
28. 28
Future work
• Prior DNA extraction
• Lyophilization of reagents (cold chain)
• Testing cidofovir as treatment for monkeypox in humans
(clinical trial)