1. The document discusses bacteriophages and their ability to infect bacteria. It describes experiments using radioactive labeling to track phage DNA and proteins during infection.
2. The structure of DNA is explained, including its phosphate backbone, nitrogenous bases, and sugar molecules. The four bases in DNA - adenine, guanine, cytosine, and thymine - are also defined.
3. DNA replication and protein synthesis are summarized, noting that replication occurs during S phase while synthesis happens during G0, G1, and G2 phases. The processes of transcription and translation are also overviewed.
This document discusses cells and their role in living organisms. It begins by explaining that organisms need to exchange nutrients through their surface area, and keeping cells small helps maintain a high surface area to volume ratio to allow for efficient nutrient exchange. It then discusses the key characteristics of prokaryotic cells, the simplest type of cell, including their lack of internal compartmentalization, circular DNA structure, rapid reproduction through binary fission, and ability to transfer genes through plasmids. The document concludes by contrasting prokaryotic and eukaryotic cells, noting key differences like prokaryotes lacking nuclei and organelles while eukaryotes have internal membrane-bound structures.
Chris Ullman, Isogenica, 'The use of CIS display for drug discovery'Cresset
The document discusses the use of CIS display for drug discovery. CIS display is an acellular, in vitro display technology that uses biochemical processes of E. coli to generate and screen large protein libraries (over 1013) without cloning. It provides advantages over other display methods like phage display by allowing larger libraries to be rapidly generated and screened. Next generation sequencing is used to monitor CIS display selections, allowing millions of sequences to be analyzed. Top hits from CIS display can then be synthesized and further matured using additional library selections and techniques to optimize binding properties.
This document summarizes research characterizing DNA methylation in the Pacific oyster Crassostrea gigas. High-throughput bisulfite sequencing was used to analyze DNA methylation patterns at high resolution. Several genes were found to have different levels and patterns of methylation across tissues and developmental stages. The results provide evidence that DNA methylation plays an important regulatory role and may be involved in environmental responses in C. gigas. Future work will investigate how epigenetic mechanisms are affected by environmental stressors.
This presentation has been moved. To view this presentation, please visit http://pubs.acs.org/iapps/liveslides/pages/index.htm?mscNo=jz300725d
Vibrational Circular Dichroism Shows Reversible Helical Handedness Switching in Peptidomimetic lValine Fibrils
A significant amount of time could be saved by cutting out the unnecessary steps from traditional cloning and moving into gene synthesis. Gene synthesis has become a costeffective, time- and resource-saving method for obtaining nearly any desired DNA construct with 100% accuracy. It outperforms conventional molecular biology techniques in terms of time and cost, while providing equivalent or better expression performance, and construct stability and quality. GeneArt® gene synthesis tools gobeyond traditional synthesis and enable expression optimization and maximum performance. Watch this webinar with audio at: http://owl.li/jppYn
Understanding and classifying metabolite space and metabolite likenessVodafoneZiggo
This document summarizes a study on classifying molecules as metabolite-like or non-metabolite using machine learning models. The study used metabolite data from HMDB and non-metabolite data from ZINC. Multiple descriptors and fingerprints were used as features to train support vector machines, random forests, and naive Bayes classifiers. The random forest model using MDL public keys achieved the best performance with high sensitivity and specificity on an external test set. The top-performing models were also able to predict metabolite-likeness for unknown structures generated in silico. However, the study found prediction was more accurate than interpretation, and local models may be needed.
This document outlines 10 hot topics in biomanufacturing: 1) product mix and business models in biopharma, 2) high-titer processes in mammalian cell culture, 3) downstream processing being driven by mass rather than volume, 4) facility fit considerations, 5) cost of goods and price margin pressures, 6) biosimilars and biobetters, 7) offshore manufacturing, 8) technology platforms and implications of high titer, 9) emerging technologies for biomass removal and contaminant clearance, and 10) single-use technologies and integrated processing. The speaker concludes by emphasizing the need for new perspectives and solutions to address challenges in biomanufacturing.
This document discusses how genetic information flows from DNA to proteins. It explains that DNA is transcribed into mRNA, which is then translated into proteins. Key points covered include:
1) The central dogma that genetic information flows from DNA to RNA to proteins.
2) Beadle and Tatum's "one gene, one enzyme" hypothesis helped establish that genes code for proteins.
3) Transcription involves using DNA as a template to make complementary mRNA strands in the nucleus, which are then processed and can leave the nucleus.
This document discusses cells and their role in living organisms. It begins by explaining that organisms need to exchange nutrients through their surface area, and keeping cells small helps maintain a high surface area to volume ratio to allow for efficient nutrient exchange. It then discusses the key characteristics of prokaryotic cells, the simplest type of cell, including their lack of internal compartmentalization, circular DNA structure, rapid reproduction through binary fission, and ability to transfer genes through plasmids. The document concludes by contrasting prokaryotic and eukaryotic cells, noting key differences like prokaryotes lacking nuclei and organelles while eukaryotes have internal membrane-bound structures.
Chris Ullman, Isogenica, 'The use of CIS display for drug discovery'Cresset
The document discusses the use of CIS display for drug discovery. CIS display is an acellular, in vitro display technology that uses biochemical processes of E. coli to generate and screen large protein libraries (over 1013) without cloning. It provides advantages over other display methods like phage display by allowing larger libraries to be rapidly generated and screened. Next generation sequencing is used to monitor CIS display selections, allowing millions of sequences to be analyzed. Top hits from CIS display can then be synthesized and further matured using additional library selections and techniques to optimize binding properties.
This document summarizes research characterizing DNA methylation in the Pacific oyster Crassostrea gigas. High-throughput bisulfite sequencing was used to analyze DNA methylation patterns at high resolution. Several genes were found to have different levels and patterns of methylation across tissues and developmental stages. The results provide evidence that DNA methylation plays an important regulatory role and may be involved in environmental responses in C. gigas. Future work will investigate how epigenetic mechanisms are affected by environmental stressors.
This presentation has been moved. To view this presentation, please visit http://pubs.acs.org/iapps/liveslides/pages/index.htm?mscNo=jz300725d
Vibrational Circular Dichroism Shows Reversible Helical Handedness Switching in Peptidomimetic lValine Fibrils
A significant amount of time could be saved by cutting out the unnecessary steps from traditional cloning and moving into gene synthesis. Gene synthesis has become a costeffective, time- and resource-saving method for obtaining nearly any desired DNA construct with 100% accuracy. It outperforms conventional molecular biology techniques in terms of time and cost, while providing equivalent or better expression performance, and construct stability and quality. GeneArt® gene synthesis tools gobeyond traditional synthesis and enable expression optimization and maximum performance. Watch this webinar with audio at: http://owl.li/jppYn
Understanding and classifying metabolite space and metabolite likenessVodafoneZiggo
This document summarizes a study on classifying molecules as metabolite-like or non-metabolite using machine learning models. The study used metabolite data from HMDB and non-metabolite data from ZINC. Multiple descriptors and fingerprints were used as features to train support vector machines, random forests, and naive Bayes classifiers. The random forest model using MDL public keys achieved the best performance with high sensitivity and specificity on an external test set. The top-performing models were also able to predict metabolite-likeness for unknown structures generated in silico. However, the study found prediction was more accurate than interpretation, and local models may be needed.
This document outlines 10 hot topics in biomanufacturing: 1) product mix and business models in biopharma, 2) high-titer processes in mammalian cell culture, 3) downstream processing being driven by mass rather than volume, 4) facility fit considerations, 5) cost of goods and price margin pressures, 6) biosimilars and biobetters, 7) offshore manufacturing, 8) technology platforms and implications of high titer, 9) emerging technologies for biomass removal and contaminant clearance, and 10) single-use technologies and integrated processing. The speaker concludes by emphasizing the need for new perspectives and solutions to address challenges in biomanufacturing.
This document discusses how genetic information flows from DNA to proteins. It explains that DNA is transcribed into mRNA, which is then translated into proteins. Key points covered include:
1) The central dogma that genetic information flows from DNA to RNA to proteins.
2) Beadle and Tatum's "one gene, one enzyme" hypothesis helped establish that genes code for proteins.
3) Transcription involves using DNA as a template to make complementary mRNA strands in the nucleus, which are then processed and can leave the nucleus.
The document discusses using DNA barcoding to identify cinnamon species. It finds that the TrnH locus amplified more successfully than MatK from cinnamon leaf DNA. Sequencing of TrnH produced high quality sequences for some species, though not all matched known sequences. Extracting DNA from cinnamon bark was unsuccessful for barcoding due to low chloroplast content and inhibitory compounds. Overall, TrnH barcoding shows promise for identifying cinnamon leaf varieties but not bark.
1) Green Pacific Biologicals has developed a genetic engineering platform that enables more stable and efficient nuclear gene expression in eukaryotic algae.
2) The platform allows for rapid genetic engineering of algae to increase carbon fixation and oil production through techniques such as increasing carbon flux into oil and implementing efficient oil secretion.
3) Modeling by Green Pacific Biologicals and a professor at Cal Poly suggests their oil secretion approach could lower the cost of algal biofuels production to $50 per barrel, compared to over $400 per barrel for conventional approaches.
The document describes tools and methods for screening compounds to find effective drugs for anxiety and depression. A typical screening process involves chemical synthesis of compounds, followed by screening assays to test binding and functional activity. Data is analyzed to select hit compounds, which then undergo further testing through ex vivo occupancy studies, in vitro ADME assays, and in vivo safety and efficacy models. Key methods discussed include radioligand binding assays to test if compounds compete for the same binding site as labeled ligands, and saturation binding assays to determine receptor affinity (Kd) and maximum binding (Bmax). Comparing results between species and receptor subtypes can provide insights into a compound's potential and selectivity.
Variability in gene expression underlies incomplete penetranceArjun Raj
This document summarizes research on the role of random gene expression variability in incomplete penetrance of mutant phenotypes in Caenorhabditis elegans. The researchers found that mutations in the skn-1 gene, which is involved in intestinal cell fate specification, resulted in variable expression of downstream genes like end-1 and elt-2 in C. elegans embryos. This led to a bimodal and probabilistic expression of the elt-2 gene, resulting in some embryos developing the mutant intestinal phenotype while others appeared normal. The variability was due to "bursty" expression of end-1, which acts as a threshold or controller of elt-2 expression levels. Reducing the variability of end-1 expression by knocking down
This study analyzed the genetic diversity of two spotted salamander populations that breed in different vernal pools near Gordon College. DNA was extracted from egg masses and adult toe clippings using phenol-chloroform purification and ethanol precipitation. An optimized amplified fragment length polymorphism (AFLP) analysis revealed higher genetic diversity and three times as many egg masses in one population compared to the other, suggesting a correlation between genetic diversity and breeding success. This molecular approach provides insights into factors influencing salamander breeding patterns.
The document discusses the future prospects for bioplastics. It notes that while demand for bioplastics is projected to increase significantly in the coming years, current bioplastics mainly serve disposable applications and do not meet the needs for durable applications due to issues with hydrolytic stability, heat resistance, and other properties. However, blends of PLA and PHBV show promise for improving heat resistance over pure materials. The development of new renewable building blocks and monomers may open up new frontiers for high-performance bioplastics that can serve durable applications. The future of bioplastics will depend on moving beyond single-use applications and improving performance and prices through technology and infrastructure developments.
SBS 2011: Sensitive Cell-based and Biochemical Assays Using Epic(R) Label-fre...PerkinElmer, Inc.
Here we show how the PerkinElmer EnSpire®Multimode Plate Reader with Corning®Epic®label-free technology can be used in 96-and 384-well microplate formats to non-invasively identify and characterize multiple G protein-coupled receptor (GPCR) pathways in living cells using an orthogonal approach. Furthermore, this orthogonal platform enables the use of both label-free and labeled technologies to comprehensively identify and characterize target and ligand behavior in both cell-based and biochemical assays, with greater confidence. By successfully monitoring the ligand-induced dynamic mass redistribution (DMR) in living cells and the ligand-dependent response interrogating protein:proteinand protein:smallmolecule biomolecularinteractions, we demonstrate that this label-free technology is a comprehensive and versatile tool for GPCR research enabling the generation of physiologically-relevant data.
The document provides an overview of molecular biology and discusses several key topics:
1) It discusses how cells organize and package DNA within the nucleus and replicate it during cell division.
2) Genome organization, including introns, exons, satellites and repetitive DNA is covered. Chromatin structure and packaging is also discussed.
3) DNA interacts with proteins through specific and non-specific binding. DNA is organized into chromatin through interactions with histone proteins in eukaryotes and other proteins in prokaryotes.
Chromosomes are organized structures that package DNA and proteins in eukaryotic cells. Bacterial genetic material is concentrated in the nucleoid as a single circular DNA chromosome. Eukaryotic cells contain linear chromosomes housed within the nucleus. Chromosomes are made up of DNA, histone proteins, and non-histone proteins. They contain genes and regulatory elements and vary in structure between species.
The document provides information on the basics of molecular biology. It begins with a table comparing key attributes of eukaryotes and prokaryotes. It then defines molecular biology as the study of the molecular underpinnings of processes like DNA replication, transcription, and translation. The basic components involved are described as DNA, RNA, and proteins. DNA stores genetic information. RNA and proteins are involved in building and regulating cells. The processes of DNA replication, transcription, translation, and their roles are summarized.
This document discusses various molecular techniques used for diagnosis of infectious diseases. It notes that molecular methods are most important for pathogens that are difficult to detect by conventional methods, like Mycobacterium tuberculosis and Chlamydia trachomatis. Several amplification techniques are described, including PCR, NASBA, TBA, SDA, and LAMP. These allow detection of pathogens in clinical samples and identification of antibiotic resistance. The document also discusses probe-based methods like hybrid capture, signal amplification techniques like branched DNA, and other methods like plasmid profiling, nucleotide sequencing, and RFLP for microbial classification and epidemiological analysis.
Molecular biology is the study of molecular underpinnings of biological processes like replication, transcription, and translation. It overlaps with genetics which studies the effect of genetic differences, and biochemistry which examines the chemical processes in living things. Researchers use techniques from all three fields including expression cloning, PCR, gel electrophoresis, and various blotting techniques to study interactions between DNA, RNA, proteins, and how their functions are regulated.
This document defines key concepts in molecular biology including DNA, genes, chromosomes, loci, alleles, genetic traits, genotype and phenotype. It explains that DNA contains genetic instructions and is packaged into chromosomes. Genes are segments of DNA that carry traits from one generation to the next. The location of a gene is its locus, and variants are alleles. Genetic traits are physical characteristics resulting from gene expression. Genotype refers to genetic makeup while phenotype describes observable traits. The document also discusses homologous chromosomes, homozygosity, heterozygosity, polymorphisms, mutations, sequence repeats, SNPs, and methods for detecting SNPs including DNA extraction, PCR, and RFLP analysis.
Chromosomes contain an organism's genetic material and come in different structures depending on the organism. Bacteria typically have a single circular chromosome while eukaryotes have multiple linear chromosomes in the nucleus. Genetic material is highly compacted through various mechanisms to fit inside cells. In eukaryotes, DNA is wrapped around histone proteins to form nucleosomes, which further compact to form a 30nm fiber and loop domains that attach to a nuclear matrix, compacting the DNA over 1000-fold to fit in the nucleus.
well, dis z again another ppt on molecular biology..
I know dis kinda luks boring bt pretty informative
thanks
let me know wat you think abt dis
don't forget to comment
This document provides an introduction to the subject of cell biology. It outlines the learning objectives which are to understand basic cell biology concepts, how molecules cooperate to create living systems, and core cell biology principles. The document also describes the various topics that will be covered in the course, including cellular structures and functions, as well as expectations for students to gain essential knowledge and apply concepts in cell biology.
RNA- A polymer of ribonucleotides, is a single stranded structure. There are three major types of RNA- m RNA,t RNA and r RNA. Besides that there are small nuclear,micro RNAs, small interfering and heterogeneous RNAs. Each of them has a specific structure and performs a specific function.
UiPath Test Automation using UiPath Test Suite series, part 5DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 5. In this session, we will cover CI/CD with devops.
Topics covered:
CI/CD with in UiPath
End-to-end overview of CI/CD pipeline with Azure devops
Speaker:
Lyndsey Byblow, Test Suite Sales Engineer @ UiPath, Inc.
Maruthi Prithivirajan, Head of ASEAN & IN Solution Architecture, Neo4j
Get an inside look at the latest Neo4j innovations that enable relationship-driven intelligence at scale. Learn more about the newest cloud integrations and product enhancements that make Neo4j an essential choice for developers building apps with interconnected data and generative AI.
The document discusses using DNA barcoding to identify cinnamon species. It finds that the TrnH locus amplified more successfully than MatK from cinnamon leaf DNA. Sequencing of TrnH produced high quality sequences for some species, though not all matched known sequences. Extracting DNA from cinnamon bark was unsuccessful for barcoding due to low chloroplast content and inhibitory compounds. Overall, TrnH barcoding shows promise for identifying cinnamon leaf varieties but not bark.
1) Green Pacific Biologicals has developed a genetic engineering platform that enables more stable and efficient nuclear gene expression in eukaryotic algae.
2) The platform allows for rapid genetic engineering of algae to increase carbon fixation and oil production through techniques such as increasing carbon flux into oil and implementing efficient oil secretion.
3) Modeling by Green Pacific Biologicals and a professor at Cal Poly suggests their oil secretion approach could lower the cost of algal biofuels production to $50 per barrel, compared to over $400 per barrel for conventional approaches.
The document describes tools and methods for screening compounds to find effective drugs for anxiety and depression. A typical screening process involves chemical synthesis of compounds, followed by screening assays to test binding and functional activity. Data is analyzed to select hit compounds, which then undergo further testing through ex vivo occupancy studies, in vitro ADME assays, and in vivo safety and efficacy models. Key methods discussed include radioligand binding assays to test if compounds compete for the same binding site as labeled ligands, and saturation binding assays to determine receptor affinity (Kd) and maximum binding (Bmax). Comparing results between species and receptor subtypes can provide insights into a compound's potential and selectivity.
Variability in gene expression underlies incomplete penetranceArjun Raj
This document summarizes research on the role of random gene expression variability in incomplete penetrance of mutant phenotypes in Caenorhabditis elegans. The researchers found that mutations in the skn-1 gene, which is involved in intestinal cell fate specification, resulted in variable expression of downstream genes like end-1 and elt-2 in C. elegans embryos. This led to a bimodal and probabilistic expression of the elt-2 gene, resulting in some embryos developing the mutant intestinal phenotype while others appeared normal. The variability was due to "bursty" expression of end-1, which acts as a threshold or controller of elt-2 expression levels. Reducing the variability of end-1 expression by knocking down
This study analyzed the genetic diversity of two spotted salamander populations that breed in different vernal pools near Gordon College. DNA was extracted from egg masses and adult toe clippings using phenol-chloroform purification and ethanol precipitation. An optimized amplified fragment length polymorphism (AFLP) analysis revealed higher genetic diversity and three times as many egg masses in one population compared to the other, suggesting a correlation between genetic diversity and breeding success. This molecular approach provides insights into factors influencing salamander breeding patterns.
The document discusses the future prospects for bioplastics. It notes that while demand for bioplastics is projected to increase significantly in the coming years, current bioplastics mainly serve disposable applications and do not meet the needs for durable applications due to issues with hydrolytic stability, heat resistance, and other properties. However, blends of PLA and PHBV show promise for improving heat resistance over pure materials. The development of new renewable building blocks and monomers may open up new frontiers for high-performance bioplastics that can serve durable applications. The future of bioplastics will depend on moving beyond single-use applications and improving performance and prices through technology and infrastructure developments.
SBS 2011: Sensitive Cell-based and Biochemical Assays Using Epic(R) Label-fre...PerkinElmer, Inc.
Here we show how the PerkinElmer EnSpire®Multimode Plate Reader with Corning®Epic®label-free technology can be used in 96-and 384-well microplate formats to non-invasively identify and characterize multiple G protein-coupled receptor (GPCR) pathways in living cells using an orthogonal approach. Furthermore, this orthogonal platform enables the use of both label-free and labeled technologies to comprehensively identify and characterize target and ligand behavior in both cell-based and biochemical assays, with greater confidence. By successfully monitoring the ligand-induced dynamic mass redistribution (DMR) in living cells and the ligand-dependent response interrogating protein:proteinand protein:smallmolecule biomolecularinteractions, we demonstrate that this label-free technology is a comprehensive and versatile tool for GPCR research enabling the generation of physiologically-relevant data.
The document provides an overview of molecular biology and discusses several key topics:
1) It discusses how cells organize and package DNA within the nucleus and replicate it during cell division.
2) Genome organization, including introns, exons, satellites and repetitive DNA is covered. Chromatin structure and packaging is also discussed.
3) DNA interacts with proteins through specific and non-specific binding. DNA is organized into chromatin through interactions with histone proteins in eukaryotes and other proteins in prokaryotes.
Chromosomes are organized structures that package DNA and proteins in eukaryotic cells. Bacterial genetic material is concentrated in the nucleoid as a single circular DNA chromosome. Eukaryotic cells contain linear chromosomes housed within the nucleus. Chromosomes are made up of DNA, histone proteins, and non-histone proteins. They contain genes and regulatory elements and vary in structure between species.
The document provides information on the basics of molecular biology. It begins with a table comparing key attributes of eukaryotes and prokaryotes. It then defines molecular biology as the study of the molecular underpinnings of processes like DNA replication, transcription, and translation. The basic components involved are described as DNA, RNA, and proteins. DNA stores genetic information. RNA and proteins are involved in building and regulating cells. The processes of DNA replication, transcription, translation, and their roles are summarized.
This document discusses various molecular techniques used for diagnosis of infectious diseases. It notes that molecular methods are most important for pathogens that are difficult to detect by conventional methods, like Mycobacterium tuberculosis and Chlamydia trachomatis. Several amplification techniques are described, including PCR, NASBA, TBA, SDA, and LAMP. These allow detection of pathogens in clinical samples and identification of antibiotic resistance. The document also discusses probe-based methods like hybrid capture, signal amplification techniques like branched DNA, and other methods like plasmid profiling, nucleotide sequencing, and RFLP for microbial classification and epidemiological analysis.
Molecular biology is the study of molecular underpinnings of biological processes like replication, transcription, and translation. It overlaps with genetics which studies the effect of genetic differences, and biochemistry which examines the chemical processes in living things. Researchers use techniques from all three fields including expression cloning, PCR, gel electrophoresis, and various blotting techniques to study interactions between DNA, RNA, proteins, and how their functions are regulated.
This document defines key concepts in molecular biology including DNA, genes, chromosomes, loci, alleles, genetic traits, genotype and phenotype. It explains that DNA contains genetic instructions and is packaged into chromosomes. Genes are segments of DNA that carry traits from one generation to the next. The location of a gene is its locus, and variants are alleles. Genetic traits are physical characteristics resulting from gene expression. Genotype refers to genetic makeup while phenotype describes observable traits. The document also discusses homologous chromosomes, homozygosity, heterozygosity, polymorphisms, mutations, sequence repeats, SNPs, and methods for detecting SNPs including DNA extraction, PCR, and RFLP analysis.
Chromosomes contain an organism's genetic material and come in different structures depending on the organism. Bacteria typically have a single circular chromosome while eukaryotes have multiple linear chromosomes in the nucleus. Genetic material is highly compacted through various mechanisms to fit inside cells. In eukaryotes, DNA is wrapped around histone proteins to form nucleosomes, which further compact to form a 30nm fiber and loop domains that attach to a nuclear matrix, compacting the DNA over 1000-fold to fit in the nucleus.
well, dis z again another ppt on molecular biology..
I know dis kinda luks boring bt pretty informative
thanks
let me know wat you think abt dis
don't forget to comment
This document provides an introduction to the subject of cell biology. It outlines the learning objectives which are to understand basic cell biology concepts, how molecules cooperate to create living systems, and core cell biology principles. The document also describes the various topics that will be covered in the course, including cellular structures and functions, as well as expectations for students to gain essential knowledge and apply concepts in cell biology.
RNA- A polymer of ribonucleotides, is a single stranded structure. There are three major types of RNA- m RNA,t RNA and r RNA. Besides that there are small nuclear,micro RNAs, small interfering and heterogeneous RNAs. Each of them has a specific structure and performs a specific function.
UiPath Test Automation using UiPath Test Suite series, part 5DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 5. In this session, we will cover CI/CD with devops.
Topics covered:
CI/CD with in UiPath
End-to-end overview of CI/CD pipeline with Azure devops
Speaker:
Lyndsey Byblow, Test Suite Sales Engineer @ UiPath, Inc.
Maruthi Prithivirajan, Head of ASEAN & IN Solution Architecture, Neo4j
Get an inside look at the latest Neo4j innovations that enable relationship-driven intelligence at scale. Learn more about the newest cloud integrations and product enhancements that make Neo4j an essential choice for developers building apps with interconnected data and generative AI.
Climate Impact of Software Testing at Nordic Testing DaysKari Kakkonen
My slides at Nordic Testing Days 6.6.2024
Climate impact / sustainability of software testing discussed on the talk. ICT and testing must carry their part of global responsibility to help with the climat warming. We can minimize the carbon footprint but we can also have a carbon handprint, a positive impact on the climate. Quality characteristics can be added with sustainability, and then measured continuously. Test environments can be used less, and in smaller scale and on demand. Test techniques can be used in optimizing or minimizing number of tests. Test automation can be used to speed up testing.
HCL Notes and Domino License Cost Reduction in the World of DLAUpanagenda
Webinar Recording: https://www.panagenda.com/webinars/hcl-notes-and-domino-license-cost-reduction-in-the-world-of-dlau/
The introduction of DLAU and the CCB & CCX licensing model caused quite a stir in the HCL community. As a Notes and Domino customer, you may have faced challenges with unexpected user counts and license costs. You probably have questions on how this new licensing approach works and how to benefit from it. Most importantly, you likely have budget constraints and want to save money where possible. Don’t worry, we can help with all of this!
We’ll show you how to fix common misconfigurations that cause higher-than-expected user counts, and how to identify accounts which you can deactivate to save money. There are also frequent patterns that can cause unnecessary cost, like using a person document instead of a mail-in for shared mailboxes. We’ll provide examples and solutions for those as well. And naturally we’ll explain the new licensing model.
Join HCL Ambassador Marc Thomas in this webinar with a special guest appearance from Franz Walder. It will give you the tools and know-how to stay on top of what is going on with Domino licensing. You will be able lower your cost through an optimized configuration and keep it low going forward.
These topics will be covered
- Reducing license cost by finding and fixing misconfigurations and superfluous accounts
- How do CCB and CCX licenses really work?
- Understanding the DLAU tool and how to best utilize it
- Tips for common problem areas, like team mailboxes, functional/test users, etc
- Practical examples and best practices to implement right away
Communications Mining Series - Zero to Hero - Session 1DianaGray10
This session provides introduction to UiPath Communication Mining, importance and platform overview. You will acquire a good understand of the phases in Communication Mining as we go over the platform with you. Topics covered:
• Communication Mining Overview
• Why is it important?
• How can it help today’s business and the benefits
• Phases in Communication Mining
• Demo on Platform overview
• Q/A
Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!SOFTTECHHUB
As the digital landscape continually evolves, operating systems play a critical role in shaping user experiences and productivity. The launch of Nitrux Linux 3.5.0 marks a significant milestone, offering a robust alternative to traditional systems such as Windows 11. This article delves into the essence of Nitrux Linux 3.5.0, exploring its unique features, advantages, and how it stands as a compelling choice for both casual users and tech enthusiasts.
Observability Concepts EVERY Developer Should Know -- DeveloperWeek Europe.pdfPaige Cruz
Monitoring and observability aren’t traditionally found in software curriculums and many of us cobble this knowledge together from whatever vendor or ecosystem we were first introduced to and whatever is a part of your current company’s observability stack.
While the dev and ops silo continues to crumble….many organizations still relegate monitoring & observability as the purview of ops, infra and SRE teams. This is a mistake - achieving a highly observable system requires collaboration up and down the stack.
I, a former op, would like to extend an invitation to all application developers to join the observability party will share these foundational concepts to build on:
Best 20 SEO Techniques To Improve Website Visibility In SERPPixlogix Infotech
Boost your website's visibility with proven SEO techniques! Our latest blog dives into essential strategies to enhance your online presence, increase traffic, and rank higher on search engines. From keyword optimization to quality content creation, learn how to make your site stand out in the crowded digital landscape. Discover actionable tips and expert insights to elevate your SEO game.
TrustArc Webinar - 2024 Global Privacy SurveyTrustArc
How does your privacy program stack up against your peers? What challenges are privacy teams tackling and prioritizing in 2024?
In the fifth annual Global Privacy Benchmarks Survey, we asked over 1,800 global privacy professionals and business executives to share their perspectives on the current state of privacy inside and outside of their organizations. This year’s report focused on emerging areas of importance for privacy and compliance professionals, including considerations and implications of Artificial Intelligence (AI) technologies, building brand trust, and different approaches for achieving higher privacy competence scores.
See how organizational priorities and strategic approaches to data security and privacy are evolving around the globe.
This webinar will review:
- The top 10 privacy insights from the fifth annual Global Privacy Benchmarks Survey
- The top challenges for privacy leaders, practitioners, and organizations in 2024
- Key themes to consider in developing and maintaining your privacy program
Dr. Sean Tan, Head of Data Science, Changi Airport Group
Discover how Changi Airport Group (CAG) leverages graph technologies and generative AI to revolutionize their search capabilities. This session delves into the unique search needs of CAG’s diverse passengers and customers, showcasing how graph data structures enhance the accuracy and relevance of AI-generated search results, mitigating the risk of “hallucinations” and improving the overall customer journey.
GraphRAG for Life Science to increase LLM accuracyTomaz Bratanic
GraphRAG for life science domain, where you retriever information from biomedical knowledge graphs using LLMs to increase the accuracy and performance of generated answers
In his public lecture, Christian Timmerer provides insights into the fascinating history of video streaming, starting from its humble beginnings before YouTube to the groundbreaking technologies that now dominate platforms like Netflix and ORF ON. Timmerer also presents provocative contributions of his own that have significantly influenced the industry. He concludes by looking at future challenges and invites the audience to join in a discussion.
Unlocking Productivity: Leveraging the Potential of Copilot in Microsoft 365, a presentation by Christoforos Vlachos, Senior Solutions Manager – Modern Workplace, Uni Systems
3. Fig. 10-1b
Radioactive Empty
Phage
protein Radioactivity
protein shell
in liquid
Bacterium Phage
DNA
DNA
Batch 1
Radioactive
protein Centrifuge
Pellet
2 Agitate in a blender to 3 Centrifuge the mixture 4 Measure the
1 Mix radioactively
separate phages so bacteria form a
labeled phages with radioactivity in
outside the bacteria pellet at the bottom of
bacteria. The phages the pellet and
from the cells and the test tube.
infect the bacterial cells. the liquid.
their contents.
Radioactive
DNA
Batch 2
Radioactive
DNA Centrifuge
Radioactivity
in pellet
Pellet
20. Fig. 10-9b
RNA polymerase
DNA of gene
Promoter Terminator
DNA DNA
1
Initiation
Area shown
2
in Figure 10.9A
Elongation
Growing
3
RNA
Termination
Completed
RNA RNA
polymerase
22. Fig. 10-12a
Growing
tRNA polypeptide
molecules
Large
subunit
mRNA Small
subunit
23. Fig. 10-12c
Next amino acid
to be added to
polypeptide
Growing
polypeptide
tRNA
mRNA
Codons
24. Fig. 10-14-4
Amino
acid
Polypeptide
A site
P site
Anticodon
mRNA Codons
1 Codon recognition
mRNA
movement
Stop
codon
2 Peptide bond
formation
New
peptide
bond
3 Translocation