Accordind to my research

1. Regional Food Research and Analysis centre
Lucknow Uttar Pradesh
Under Guidance of- Submitted by-
Dr. S.K. Chauhan Mayank Agnihotri
Director, R-Frac Lucknow Chhatrapati Shahu Ji
Maharaj University
Kanpur
Uttar Pradesh

2. introduction
 Juice is the liquid that is naturally enclosed in fruit or
vegetable tissues
 To determine the levels of total aerobic viable bacteria,
staphylococci, total aerobic spore forming bacteria,
total coliforms, fecal coliforms and yeast and mold in
the fresh fruit juice samples

3. Methods and materials
 To analyzing juice quality with in microbial parameter
 Two fruit juice samples (apple and orange) were
collected from 4 randomly selected area in lucknow
(charabag railway station and shapru marg ) between
may 2017 to July 2017
s.no sample Collection area
1 apple Charbagh railway station
2 Orange Shapru marg

4. Microbial analysis
Under Microbiological Analysis we make following
Tests:
 T.P.C ( total plate count )
 Coli form
 Yeast & Mold count
 E.coli
 Salmonella

5. Total plate count
 Reference IS: 5402, 2002
 Media used- plate count agar
 Orange Juice -
 Result : 4.3 x 102 cfu/ml
 Apple juice –
 Results : 3.8 x 102 cfu/ml

6. Coliforms
 Reference IS: 5887(P-1) 1976
 Pour plate method:-
 Media used: violet red bile agar media
 Orange juice:-
 Result:-Coli form in orange juice is absent
 Apple juice:-
 Result:-Coli form in Apple juice is absent

7. Yeast & molds
 Reference IS: 5401(P-1) 1998
 Pour plate method:-
 Media used:- YXT Agar media.
 Orange juice:-
 Results:- Y & M = 1.3 x 103 cfu/ml
 Apple Juice : -
 Results:- Y & M = 2.1 x 103CFU/ml

8. E.coli
 Reference IS: 5887(P-2) 1976
 Pour plate method:-
 Media used:- EMB Agar media
 Orange juice :-
 Result :-E coli in orange juice is absent
 Apple juice:-
 Result :-E coli in Apple juice is absent

9. salmonella
 Reference IS: 5887(P-3) 1976
 Strike plate method:-
 Media used:- Brilliant Green Agar media.
 Orange juice:-
 Result :- Salmonella is present
 Apple juice:-
 Result :- Salmonella is present

10. Results & discussion
sample T.P.C Coli form Yeast &
molds
E.coli salmonell
a
Apple 3.8 x 102
cfu/ml
Absent Y & M = 2.1
x
103CFU/ml
Absent Present
orange 4.3 x 102
cfu/ml
Absent Y & M = 1.3
x 103 cfu/ml
Absent Present

11. conclusion
 The fruit juices investigated in this study had higher microbial load than the
specifications set for fruit juices in some parts of the world.
 On the basis of the gulf standards, it is clear that the colony counts of the microbial
groups in our fruit juices exceeded the standard by considerable margin.
 The presence of pathogenic microorganisms in juices is clearly indication of food
borne outbreaks.