1) Invasive mussels are a major problem for water facilities and their shells can clog pipes and screens. Chemical controls kill mussels all at once, releasing shell debris.
2) A new product derived from soil bacteria selectively kills mussels over time, reducing shell debris impacts. Extensive testing shows no effects on fish, plants, or other species.
3) Treatment programs are designed to further mitigate debris by limiting mussel size or slowing mortality, allowing facilities to better manage shell buildup. Pilot studies demonstrate the product's efficacy in controlling mussels and debris.
This document discusses the selection of biological methods for assessing the quality of industrial effluents. It identifies existing bioassays for evaluating acute and chronic toxicity of wastewater. Biological methods can be divided into microbiological, limnological, and ecotoxicological tests. Common ecotoxicological tests identified include those using algae, microcrustaceans like Daphnia, fish, and bacteria to analyze effluent toxicity. The document compares different test methods and variables for routinely monitoring effluents and researching their impacts.
Improving microalgae for biotechnology - from genetics to synthetic biologynirvarna gr
The document discusses genetic engineering approaches for microalgae. It describes forward genetics methods like chemical and physical mutagenesis that generate random mutants for screening. It also describes reverse genetics methods like insertional mutagenesis that allow targeted modification of specific genes. The goal is to generate strains with desired traits like increased lipid or starch production for industrial applications.
8- IJRANSS-ANTIMICROBIAL POTENTIAL OF MARINE ACTINOMYCETESRavindragouda Patil
- 47 actinomycetes were isolated from mangrove swamp samples in Tamil Nadu, India, with most isolated from sediment.
- 34 isolates exhibited antagonism against shrimp pathogens Vibrio alginolyticus, V. harveyi, and V. parahaemolyticus.
- Isolate A10 showed the strongest inhibitory activity against all three pathogens, inhibiting growth by ≥20mm.
- Isolate A10 was identified as Streptomyces spp. based on chemotaxonomic and microscopic analysis.
This document discusses how biomarkers found in saliva can benefit research programs in behavioral health and sports sciences. It describes how saliva is a minimally invasive sample that is easy and safe to collect. The document outlines various analytes found in saliva, such as cortisol, cytokines, antibodies, and drugs. It discusses the movement of biomarkers from blood into saliva and notes that some analytes are locally produced in the oral cavity. The collection, storage, and analysis of salivary samples is also reviewed. Overall, the document promotes the integration of salivary bioscience into behavioral research.
Few species are standardized and have been used as test organisms around the world in
ecotoxicological assays. In the case of sediment assessment, there are only two amphipod species
(Tiburonella viscana and Grandidierella bonnieroides) standardized protocols for toxicity test in South
Atlantic region.
This document provides information about freshwater mussels in the Chehalis River Basin. It discusses that the Chehalis Basin is home to several species of native freshwater mussels, including the Western Pearlshell and Western Ridged Mussel. Mussels play an important ecological role by filtering water to improve quality. They are also an indicator of habitat health but many species are endangered or extinct across North America due to factors like dams, pollution, and habitat loss. Conservation efforts aim to map and monitor mussel populations to inform protection strategies. The document warns about invasive mussel species not currently found in Washington that could harm local ecosystems.
The zebra mussel is a small freshwater mussel that has invaded waterways across North America. It attaches using strong fibers and can filter large amounts of water, removing phytoplankton that forms the base of the aquatic food web. This has negatively impacted native species and ecosystems by reducing food sources, encrusting surfaces of other mussels and infrastructure, and allowing algal blooms. The zebra mussel was introduced from parts of Russia and has caused extensive environmental damage since becoming established outside its native range.
Dick Sternberg Zebra Mussel PresentationGlen Simons
This document summarizes information about zebra mussels, an invasive species that has caused significant ecological and economic damage after being introduced to North American waters. Some key points:
- Zebra mussels were introduced in the late 1980s and have since spread rapidly across many waterways, attaching to surfaces and filtering plankton that native species rely on.
- Their proliferation has disrupted aquatic food webs and ecosystems. It has reduced populations of native mussels, fish, and birds that have been poisoned by toxins concentrated up the food chain.
- They clog pipes, damage boats and infrastructure, and harm industries like power generation that rely on intake water. Their estimated U.S. economic costs
This document discusses the selection of biological methods for assessing the quality of industrial effluents. It identifies existing bioassays for evaluating acute and chronic toxicity of wastewater. Biological methods can be divided into microbiological, limnological, and ecotoxicological tests. Common ecotoxicological tests identified include those using algae, microcrustaceans like Daphnia, fish, and bacteria to analyze effluent toxicity. The document compares different test methods and variables for routinely monitoring effluents and researching their impacts.
Improving microalgae for biotechnology - from genetics to synthetic biologynirvarna gr
The document discusses genetic engineering approaches for microalgae. It describes forward genetics methods like chemical and physical mutagenesis that generate random mutants for screening. It also describes reverse genetics methods like insertional mutagenesis that allow targeted modification of specific genes. The goal is to generate strains with desired traits like increased lipid or starch production for industrial applications.
8- IJRANSS-ANTIMICROBIAL POTENTIAL OF MARINE ACTINOMYCETESRavindragouda Patil
- 47 actinomycetes were isolated from mangrove swamp samples in Tamil Nadu, India, with most isolated from sediment.
- 34 isolates exhibited antagonism against shrimp pathogens Vibrio alginolyticus, V. harveyi, and V. parahaemolyticus.
- Isolate A10 showed the strongest inhibitory activity against all three pathogens, inhibiting growth by ≥20mm.
- Isolate A10 was identified as Streptomyces spp. based on chemotaxonomic and microscopic analysis.
This document discusses how biomarkers found in saliva can benefit research programs in behavioral health and sports sciences. It describes how saliva is a minimally invasive sample that is easy and safe to collect. The document outlines various analytes found in saliva, such as cortisol, cytokines, antibodies, and drugs. It discusses the movement of biomarkers from blood into saliva and notes that some analytes are locally produced in the oral cavity. The collection, storage, and analysis of salivary samples is also reviewed. Overall, the document promotes the integration of salivary bioscience into behavioral research.
Few species are standardized and have been used as test organisms around the world in
ecotoxicological assays. In the case of sediment assessment, there are only two amphipod species
(Tiburonella viscana and Grandidierella bonnieroides) standardized protocols for toxicity test in South
Atlantic region.
This document provides information about freshwater mussels in the Chehalis River Basin. It discusses that the Chehalis Basin is home to several species of native freshwater mussels, including the Western Pearlshell and Western Ridged Mussel. Mussels play an important ecological role by filtering water to improve quality. They are also an indicator of habitat health but many species are endangered or extinct across North America due to factors like dams, pollution, and habitat loss. Conservation efforts aim to map and monitor mussel populations to inform protection strategies. The document warns about invasive mussel species not currently found in Washington that could harm local ecosystems.
The zebra mussel is a small freshwater mussel that has invaded waterways across North America. It attaches using strong fibers and can filter large amounts of water, removing phytoplankton that forms the base of the aquatic food web. This has negatively impacted native species and ecosystems by reducing food sources, encrusting surfaces of other mussels and infrastructure, and allowing algal blooms. The zebra mussel was introduced from parts of Russia and has caused extensive environmental damage since becoming established outside its native range.
Dick Sternberg Zebra Mussel PresentationGlen Simons
This document summarizes information about zebra mussels, an invasive species that has caused significant ecological and economic damage after being introduced to North American waters. Some key points:
- Zebra mussels were introduced in the late 1980s and have since spread rapidly across many waterways, attaching to surfaces and filtering plankton that native species rely on.
- Their proliferation has disrupted aquatic food webs and ecosystems. It has reduced populations of native mussels, fish, and birds that have been poisoned by toxins concentrated up the food chain.
- They clog pipes, damage boats and infrastructure, and harm industries like power generation that rely on intake water. Their estimated U.S. economic costs
This document discusses using aquatic macroinvertebrates to monitor water quality. It outlines how macroinvertebrates can indicate the health of bodies of water, explains how they are classified by their tolerance to pollution, and describes how to sample, sort, identify, and calculate a Benthic Index of Biotic Integrity score. The sampling process involves collecting macroinvertebrates from different habitats using nets and grab samplers, preserving the samples, sorting and identifying the organisms, and calculating the score to assess the water quality and detect problems.
This document provides information from a presentation on internal parasites for sheep given at the Meat Sheep Alliance of Florida Fall Symposium. The presentation covered topics including the American Consortium for Small Ruminant Parasite Control, an overview of common parasites, anthelmintics (dewormers), anthelmintic resistance, and alternative parasite control methods. It discussed common gastrointestinal parasites of sheep, described various anthelmintic drug classes and resistance issues, and emphasized the importance of integrated parasite management to prevent further development of anthelmintic resistance.
This document discusses strategies for controlling gastrointestinal parasites like Haemonchus contortus (barber pole worm) in small ruminants. It emphasizes using integrated and targeted approaches like selective deworming based on FAMACHA scores, pasture management, and limiting drug use to reduce anthelmintic resistance. Proper administration and alternating between drug classes is also important for effective control. Sustainable practices involve preventing environmental contamination through various animal health and grazing techniques.
Role of Bio Markers In Water MonitoringMOHTISHIM CH
Biological monitoring uses organisms to assess environmental changes, especially those caused by human activity. Biomarkers are species that indicate ecosystem status through physiological or behavioral responses. Biomonitoring involves using indicator species like benthic macroinvertebrates, fish, algae, and plants to monitor water quality at individual, population, and community levels. Common biomonitoring techniques sample organisms or their tissues to detect genetic, reproductive, or other biological impacts from pollution.
The document discusses managing common greenhouse pests like whiteflies, aphids, and thrips using integrated pest management strategies, which include monitoring pest populations, identifying pests, and using cultural, biological, and chemical control methods like beneficial insects, insecticides applied through drip irrigation systems, and sanitation to control pests below economic thresholds. It provides examples of specific IPM programs for managing pests on cucumbers, tomatoes, and brassicas using drip-applied neonicotinoid and diamide insecticides.
This document discusses potential future solutions for managing aquatic weeds based on experiences in Australia and New Zealand. It describes how aquatic plants can be both useful and problematic in overgrowth. Common aquatic weeds and reasons for controlling them are outlined. Methods for control include various herbicides as well as mechanical and physical removal techniques. Case studies demonstrate successful control of specific weeds like Egeria, Cabomba, and Hydrilla using herbicides in hydrogel formulations or whole lake treatments. Overall, herbicides are seen as useful when applied correctly by experts, but all aquatic weed control requires a tailored approach.
Integrated vector control approach Dr Kulrajat Bhasin.drkulrajat
The document discusses integrated vector control (IVC), which combines multiple control methods to maximize results while minimizing effort. IVC is the trend for mosquito control, utilizing appropriate technologies and management techniques cost-effectively while avoiding overuse of any single method. Selection of control measures requires detailed field information on vector ecology and disease transmission role. Key elements of an IVC strategy include source reduction, biological controls like larvivorous fish, chemical controls such as insect growth regulators, and health education on personal protection measures.
MANAGEMENT OF WETLANDS THROUGH BIOMANIPULATIONSalmashaik26
This document summarizes management of wetlands through biomanipulation, which involves top-down and bottom-up methods to control algal blooms and restore water quality. Biomanipulation techniques include increasing predators to control planktivorous fish and zooplankton populations. Bottom-up methods aim to reduce nutrient levels, while top-down methods alter higher trophic levels to control lower ones. Both approaches together can effectively address eutrophication issues in wetlands and shallow waters.
The document discusses parasites and goats at the Dakota Goat Association's statewide conference. It provides an overview of the American Consortium for Small Ruminant Parasite Control, which develops novel parasite control methods and educates producers. Key topics covered include the biology of parasites, anthelmintics (dewormers), anthelmintic resistance, and integrated parasite control strategies like combination treatments and copper oxide wire particles.
Living machine: The Ecological Wastewater TreatmentSagar Patel
1. Living machine technology is a patented form of ecological wastewater treatment that mimics the cleansing functions of wetlands. It was developed by Dr. John Todd and is based on fixed-film ecology and the processes of natural tidal wetlands.
2. The technology uses 12 principles of ecological design, including mineral and nutrient diversity, steep gradients, high exchange rates, periodic pulses, a cellular structure, subecosystems, microbial communities, and photosynthetic and animal diversity.
3. Examples of how these principles are applied include using rocks to provide minerals, ecological fluidized beds for exchange rates, plant and algae systems for photosynthesis, and snails and other animals that graze algae and
Plankton are small organisms that drift or float in aquatic environments such as oceans, seas and bodies of fresh water. They play an important role in aquatic food webs as primary producers (phytoplankton) or primary consumers (zooplankton). Phytoplankton include algae like diatoms, dinoflagellates and cyanobacteria which produce oxygen and serve as the base of the food chain. Zooplankton include protozoa, rotifers, crustaceans and copepods which feed on phytoplankton and bacteria and are food for larger organisms. Plankton are crucial for marine ecosystems as they provide the base of the food web that supports many fish
Mey Akashah "Sustainable Seas and Seafood," Harvard Mey Akashah
Mey Akashah, "Sustainable Seas and Sustainable Seafood," Harvard School of Public Health, Harvard Medical School, and Harvard Extension School, May 1 2012.
Course: Human Health and Global Environmental Change
Dunn, Heidi, Ecological Specialists, Inc., Freshwater Mussels and the New Amm...Kevin Perry
Freshwater mussels are an important part of river ecosystems but many species are endangered. Mussels play key roles like filtering water and providing habitat. They are very sensitive to pollutants like ammonia and were among the most sensitive species considered in setting the 2013 US EPA ammonia standard. This standard will better protect mussels and other aquatic life in Missouri waters, though it may apply broadly across the state given mussels' widespread historical distribution. Cooperation will be important to successfully implement the new protective standard.
This document describes various coastal ecosystems including rocky shores, coral reefs, mangroves, salt marshes, kelp forests, and sea grass beds. It then discusses the primary producers that form the base of the food web in coastal ecosystems, including various types of phytoplankton, cyanobacteria, algae, seagrasses, and mangroves. Finally, it provides information on adaptations of marine organisms that inhabit coastal ecosystems.
This document discusses microbial adaptation to aquatic environments. It explains that bacterial size, nutrients, light, oxygen, and other factors affect microbial diversity. Most bacteria are 0.2 um in diameter but some have adapted to be smaller or larger. Ultramicrobacteria can be as small as 0.2 um and maximize surface area for nutrient absorption. The largest known bacteria is Thiomargarita namibiensis, which can be 750 um wide and uses sulfur and nitrates through a large internal vacuole. Thioploca spp. also live in marine sediments and bring together sulfur and nitrates through tubular sheaths. Fungi like chytrids and Ingoldian fungi can reproduce underwater through spores and
Biological Characteristics Of Cultured Species Thanh Asean TrainingRidzaludin
The document discusses the biological characteristics and species selection for aquaculture of several major cultured species. It provides details on the reproduction, growth, feeding habits and environmental requirements of species like river catfish, tilapia, giant freshwater prawn and black tiger shrimp. The document also outlines objectives and factors to consider for species selection in aquaculture, including economic and biological characteristics. It lists examples of suitable freshwater and marine species for different aquaculture systems.
This document discusses using diatoms as feed for aquaculture. It notes that diatoms support productive fisheries and play a crucial role in the carbon cycle. Several species of diatoms have been sequenced, including Thalassiosira pseudonana. Benthic diatoms are the main food source for abalone post-larvae. Studies have found abalone fed cultured diatoms have better growth than those fed natural diatoms. The document aims to improve abalone post-larval growth using specific diatom species as feed and understand their dietary requirements better.
This document summarizes a study that examined the antimicrobial properties of mucus from the chame fish (Dormitator latifrons). The study found inhibitory effects of chame mucus against several bacteria strains. Specifically, chame mucus showed inhibitory effects against 2 out of 3 Bacillus strains tested as well as strong inhibitory effects against Vibrio vulnificus and Vibrio harveyi. A lower level of inhibition was also observed against Vibrio anguillarum. The results suggest the presence of antibacterial agents in chame fish mucus, which could potentially be applied to animal and human health.
Biomonitoring uses biological responses to assess environmental changes, especially those from human activities. Biomarkers and sentinel organisms that accumulate pollutants can serve as important biomonitoring tools. Common types of biomonitoring include using algae, benthic macroinvertebrates, and fish to monitor water quality. Each indicator organism has advantages like short life cycles for algae or representing different trophic levels for fish, but also disadvantages such as motility issues for accurately assessing fish population changes. A variety of sampling techniques exist for different indicator species.
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...Jeffrey Haguewood
Sidekick Solutions uses Bonterra Impact Management (fka Social Solutions Apricot) and automation solutions to integrate data for business workflows.
We believe integration and automation are essential to user experience and the promise of efficient work through technology. Automation is the critical ingredient to realizing that full vision. We develop integration products and services for Bonterra Case Management software to support the deployment of automations for a variety of use cases.
This video focuses on integration of Salesforce with Bonterra Impact Management.
Interested in deploying an integration with Salesforce for Bonterra Impact Management? Contact us at sales@sidekicksolutionsllc.com to discuss next steps.
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This document discusses using aquatic macroinvertebrates to monitor water quality. It outlines how macroinvertebrates can indicate the health of bodies of water, explains how they are classified by their tolerance to pollution, and describes how to sample, sort, identify, and calculate a Benthic Index of Biotic Integrity score. The sampling process involves collecting macroinvertebrates from different habitats using nets and grab samplers, preserving the samples, sorting and identifying the organisms, and calculating the score to assess the water quality and detect problems.
This document provides information from a presentation on internal parasites for sheep given at the Meat Sheep Alliance of Florida Fall Symposium. The presentation covered topics including the American Consortium for Small Ruminant Parasite Control, an overview of common parasites, anthelmintics (dewormers), anthelmintic resistance, and alternative parasite control methods. It discussed common gastrointestinal parasites of sheep, described various anthelmintic drug classes and resistance issues, and emphasized the importance of integrated parasite management to prevent further development of anthelmintic resistance.
This document discusses strategies for controlling gastrointestinal parasites like Haemonchus contortus (barber pole worm) in small ruminants. It emphasizes using integrated and targeted approaches like selective deworming based on FAMACHA scores, pasture management, and limiting drug use to reduce anthelmintic resistance. Proper administration and alternating between drug classes is also important for effective control. Sustainable practices involve preventing environmental contamination through various animal health and grazing techniques.
Role of Bio Markers In Water MonitoringMOHTISHIM CH
Biological monitoring uses organisms to assess environmental changes, especially those caused by human activity. Biomarkers are species that indicate ecosystem status through physiological or behavioral responses. Biomonitoring involves using indicator species like benthic macroinvertebrates, fish, algae, and plants to monitor water quality at individual, population, and community levels. Common biomonitoring techniques sample organisms or their tissues to detect genetic, reproductive, or other biological impacts from pollution.
The document discusses managing common greenhouse pests like whiteflies, aphids, and thrips using integrated pest management strategies, which include monitoring pest populations, identifying pests, and using cultural, biological, and chemical control methods like beneficial insects, insecticides applied through drip irrigation systems, and sanitation to control pests below economic thresholds. It provides examples of specific IPM programs for managing pests on cucumbers, tomatoes, and brassicas using drip-applied neonicotinoid and diamide insecticides.
This document discusses potential future solutions for managing aquatic weeds based on experiences in Australia and New Zealand. It describes how aquatic plants can be both useful and problematic in overgrowth. Common aquatic weeds and reasons for controlling them are outlined. Methods for control include various herbicides as well as mechanical and physical removal techniques. Case studies demonstrate successful control of specific weeds like Egeria, Cabomba, and Hydrilla using herbicides in hydrogel formulations or whole lake treatments. Overall, herbicides are seen as useful when applied correctly by experts, but all aquatic weed control requires a tailored approach.
Integrated vector control approach Dr Kulrajat Bhasin.drkulrajat
The document discusses integrated vector control (IVC), which combines multiple control methods to maximize results while minimizing effort. IVC is the trend for mosquito control, utilizing appropriate technologies and management techniques cost-effectively while avoiding overuse of any single method. Selection of control measures requires detailed field information on vector ecology and disease transmission role. Key elements of an IVC strategy include source reduction, biological controls like larvivorous fish, chemical controls such as insect growth regulators, and health education on personal protection measures.
MANAGEMENT OF WETLANDS THROUGH BIOMANIPULATIONSalmashaik26
This document summarizes management of wetlands through biomanipulation, which involves top-down and bottom-up methods to control algal blooms and restore water quality. Biomanipulation techniques include increasing predators to control planktivorous fish and zooplankton populations. Bottom-up methods aim to reduce nutrient levels, while top-down methods alter higher trophic levels to control lower ones. Both approaches together can effectively address eutrophication issues in wetlands and shallow waters.
The document discusses parasites and goats at the Dakota Goat Association's statewide conference. It provides an overview of the American Consortium for Small Ruminant Parasite Control, which develops novel parasite control methods and educates producers. Key topics covered include the biology of parasites, anthelmintics (dewormers), anthelmintic resistance, and integrated parasite control strategies like combination treatments and copper oxide wire particles.
Living machine: The Ecological Wastewater TreatmentSagar Patel
1. Living machine technology is a patented form of ecological wastewater treatment that mimics the cleansing functions of wetlands. It was developed by Dr. John Todd and is based on fixed-film ecology and the processes of natural tidal wetlands.
2. The technology uses 12 principles of ecological design, including mineral and nutrient diversity, steep gradients, high exchange rates, periodic pulses, a cellular structure, subecosystems, microbial communities, and photosynthetic and animal diversity.
3. Examples of how these principles are applied include using rocks to provide minerals, ecological fluidized beds for exchange rates, plant and algae systems for photosynthesis, and snails and other animals that graze algae and
Plankton are small organisms that drift or float in aquatic environments such as oceans, seas and bodies of fresh water. They play an important role in aquatic food webs as primary producers (phytoplankton) or primary consumers (zooplankton). Phytoplankton include algae like diatoms, dinoflagellates and cyanobacteria which produce oxygen and serve as the base of the food chain. Zooplankton include protozoa, rotifers, crustaceans and copepods which feed on phytoplankton and bacteria and are food for larger organisms. Plankton are crucial for marine ecosystems as they provide the base of the food web that supports many fish
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Freshwater mussels are an important part of river ecosystems but many species are endangered. Mussels play key roles like filtering water and providing habitat. They are very sensitive to pollutants like ammonia and were among the most sensitive species considered in setting the 2013 US EPA ammonia standard. This standard will better protect mussels and other aquatic life in Missouri waters, though it may apply broadly across the state given mussels' widespread historical distribution. Cooperation will be important to successfully implement the new protective standard.
This document describes various coastal ecosystems including rocky shores, coral reefs, mangroves, salt marshes, kelp forests, and sea grass beds. It then discusses the primary producers that form the base of the food web in coastal ecosystems, including various types of phytoplankton, cyanobacteria, algae, seagrasses, and mangroves. Finally, it provides information on adaptations of marine organisms that inhabit coastal ecosystems.
This document discusses microbial adaptation to aquatic environments. It explains that bacterial size, nutrients, light, oxygen, and other factors affect microbial diversity. Most bacteria are 0.2 um in diameter but some have adapted to be smaller or larger. Ultramicrobacteria can be as small as 0.2 um and maximize surface area for nutrient absorption. The largest known bacteria is Thiomargarita namibiensis, which can be 750 um wide and uses sulfur and nitrates through a large internal vacuole. Thioploca spp. also live in marine sediments and bring together sulfur and nitrates through tubular sheaths. Fungi like chytrids and Ingoldian fungi can reproduce underwater through spores and
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This document discusses using diatoms as feed for aquaculture. It notes that diatoms support productive fisheries and play a crucial role in the carbon cycle. Several species of diatoms have been sequenced, including Thalassiosira pseudonana. Benthic diatoms are the main food source for abalone post-larvae. Studies have found abalone fed cultured diatoms have better growth than those fed natural diatoms. The document aims to improve abalone post-larval growth using specific diatom species as feed and understand their dietary requirements better.
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Biomonitoring uses biological responses to assess environmental changes, especially those from human activities. Biomarkers and sentinel organisms that accumulate pollutants can serve as important biomonitoring tools. Common types of biomonitoring include using algae, benthic macroinvertebrates, and fish to monitor water quality. Each indicator organism has advantages like short life cycles for algae or representing different trophic levels for fish, but also disadvantages such as motility issues for accurately assessing fish population changes. A variety of sampling techniques exist for different indicator species.
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3. Invasive Mussel Control Methods Must Consider
Shell Debris Management
• Shells from dead adult mussels
can clog screens or small
tubing causing similar impacts
as live mussel colonization
• Chemical solutions kill all the
mussels at the same time,
releasing masses of debris into
the treated system
• Regular treatments can help
mitigate impact of shell debris
• Settlement prevention
requires almost 24/7
continuous application
• More sustainable alternative
needed with flexibility to
improve shell debris
management
3
4. Natural Product Chemistry
• Safer alternatives to standard
chemicals to protect public health
and the environment
– Products are more target specific
– Decreased potential for population resistance
• Typically not a toxic/contact mode of action
providing a more manageable control of pests
• Well established technology in
the pharmaceutical and agricultural industries
– Bacillus thuringiensis (israelensis) used to control black fly larva
in open waters - globally
– Production of antibiotics and antifungal agents effective against
diseases in crops
4
5. Product Overview
• Derived from soil microbe
(Pseudomonas fluorescens CL 145A)
– Discovered by NYSM
– Composed of 100% dead cells
• Controls mussels in all life stages
• Perceived as food source—destroys the
mussel’s digestive system
– Causing slower mortality over time, not
all at once
• Highly selective toward zebra and
quagga mussels
• Effective in a broad range of water
conditions and temperatures
• Noncorrosive and nonvolatile
5
6. Extensive Ecotox Studies Show No Impact to
Other Aquatic Species
MOLLUSCS
FISH Blue Mussel (Mytilus edulis) *
Bluegill sunfish (Lepomis macrochirus) Freshwater Mussel - Duck Mussel (Anadonta)
Channel catfish (Ictalurus punctatus) Freshwater Mussel - Black Sandshell (Ligumia recta)
Chinook Salmon (Oncorhynchus tshawytscha) Freshwater Mussel - Fatmucket (Lampsilis siliquoidea)
Coaster brook trout (Salvelinus fontinalis) Freshwater Mussel - Pink mucket (Lampsilis abrupta)
Common Carp (Cyprinus carpio) Freshwater Mussel - Hickorynut (Obovaria olivaria)
Fathead Minnow (Pimephales promelas) * Freshwater Mussel - Higgins Eye (Lampsilis higginsii)
Klamath Suckers (Catostomus sucker spp) Freshwater Mussel - Mucket (Actinonaias ligamentina)
Lake sturgeon (Acipenser fulvescens) Freshwater Mussel - Paper Pond Shell (Utterbackia imbecillis)
Largemouth bass (Micropterus salmoides) Freshwater Mussel - Plain Pocketbook (Lampsilis cardium)
Rainbow Trout (Oncorhynchus mykiss) * Freshwater Mussel - Washboard (Megalonaias nervosa)
Sacramento Splittail (Pogonichthys macrolepidotus) Freshwater Snail (Lymnaea peregra)
Smallmouth bass (Micropterus dolomieu)
Striped Bass (Morone saxatilis)
Walleye (Sander vitreus)
Yellow perch (Perca flavescens)
PLANTS AND ALGAE
Algae *
OTHERS Bindweed (Convolvulaceae)
Mallard Duck * Common Water Plantain (Alisma subcordatum)
Midge (Chironomidae) Curly Dock (Rumex crispus)
Mayfly (Baetis) Mallow (Malvaceae)
Amphipod (Hyalella azteca) * Nightshade (Solanaceae)
European Freshwater Crayfish (Austropotatamobius pallipes) Smallflower Umbrella Sedge (Cyperus difformis)
Freshwater Crustacean (Asellus aquaticus)
Freshwater Water Flea (Daphnia magna) *
* EPA required
Studies completed or will receive final report in 2012. Conducted by Institute of Technology, Sligo, Ireland;
6 New York State Museum and USGS; U.S. Bureau of Reclamation; Certified Good Laboratory Practices (GLP) Lab; and MBI lab
7. Treatment Programs Designed to Mitigate Shell
Debris Impacts
Periodic Preventative
Designed for facilities with tolerance Goal is to limit the number of mussels
for moderate to large shell sizes (larger that exceed 4mm in size
than 4 mm in size) Ideal for sensitive systems and
Treatments occur 1 -3 times annually equipment
Modification - slower/reduced kill for Treatments are performed on a
large populations can provide regular basis (approx. every 4-6 wks)
additional mitigation Modification – More frequent
Monitoring Level of Control – treatments to further reduce shell size
Quantified by percent of mussel s that Monitoring Level of Control–
die over time Evaluation of total biomass on
settlement plates and confirmation of
no mussels > 4 mm
• Product applied with standard equipment
• Treatments can be completed within hours (~6 hrs)
7
8. Methods to Assess Treatment
• Bioboxes connected to facility’s
service water system
– Used for pilot demonstration (direct
application) and facility treatments
• Mussels collected locally for assessment
of periodic treatments or settlement
plates are inserted for assessing
preventative treatments
• Configured for comparison
between treated and control
• Product measured as turbidity
(NTU) during treatments
8
10. Case Study: Bureau of Reclamation’s Davis Dam
• Tested both Periodic and Preventative
treatment programs in 2011
• Bullhead City, AZ; Colorado River
• Five turbine, 240 MW hydropower
facility
• Each turbine has a separate cooling
water system
• 5000 gpm per cooling water subsystem
10
12. Low Dose Treatment Programs Provide Additional
Debris Management Over Time
90.0
80.0
70.0
60.0
Mortality (%)
50.0
Treated mean mortality
40.0 Control mean mortality
30.0
20.0
10.0
0.0
0 10 20 30 40 50 60 70 80 90 100 110 120
Time (days)
Initial treatment occurred at 0 days; blue arrows indicate timing
of additional lower concentration treatments which had a
12 cumulative effect on mussel mortality over time
13. Biomass Control at Mid-System Locations
0.30
Settlement Biomass (dry g/plate)
0.25
0.20
Treated Control
Settlement in Treated System at
0.15
Mid-System Location
0.10
0.05
0.00
Midpoint
• Demonstration of the preventative
treatment program
• 91.2% less biomass based on total plate
Settlement in Control System at biomass
Mid-System Location
14. Biomass Control at Outlet Locations
0.30
Settlement Biomass (dry g/plate)
0.25
Treated Control
0.20
0.15
0.10
Settlement in Treated System at Outlet
Location
0.05
0.00
Outlet
• 79.7% less biomass in the treated system
• Settlement analyzed three weeks after final
Zequanox treatment; new growth not excluded
during three-week period
Settlement in Control System at
Outlet Location
15. Summary of Davis Dam 2011 Case Studies
• Low concentration repeated treatments on adult mussels can
achieve accumulated mussel mortality over time yet reduce
influx of shell debris
• Treatments can achieve equivalent mussel control to chemical
treatments without causing shell debris to enter the system
all at once
• Successive low concentration treatments on recently settled
mussels can achieve a high level of control resulting in a
reduction of mussel biomass growth (Preventative Treatment
Program)
• Significant level of mussel control during extreme algae bloom
events
• Treatments caused no impact on Colorado River water quality
15
16. Other Benefits of Zequanox Treatments
• Minimal use restrictions
• Elimination of quenching/detoxification before discharge
• No risk of release of toxic, hazardous chemicals into the
environment
• Minimal to no discharge monitoring requirements
• Low risk to employees
– Only minimal PPE needed
• No risk of corrosion or equipment damage
• Short treatment times
• Minimal impact on process and operations
16
17. Marrone Bio Innovations
MBI discovers, develops, and markets
effective and environmentally responsible
natural products (biopesticides) that fill
unmet needs for weed, pest and plant
disease management.
We have our own microbial natural product
discovery screening
We in-license proprietary technology and
products (plant extracts and microbes), such as
Pf CL145A from NYSM (Zequanox)
17
18. Questions?
Sarahann M. Rackl, Ph.D., P.E.
Marrone Bio Innovations
srackl@marronebio.com
(530) 750–2800
18