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Laboratory Safety
Guidelines
Why does it matter?
 Safe working
protects:
 You
 Other lab workers
 Cleaners
 Visitors
 Your work
What does the law say? (1)
 Health Safety at Work etc Act
1974
 You must work safely
 You must not endanger others
 You must not misuse safety
equipment
 Penalty – up to 2 year in
prison &/or an unlimited fine
What does the law say? (2)
 The Management of
Health and Safety at
Work Regs 1999
 Control of Substances
Hazardous to Health
Regs 2004
 You must perform
RISK
ASSESSMENTS
How to do a Risk Assessment?
 Determine hazards and evaluate
risks
 Use all relevant available data
 Determine controls needed to
minimise those risks
 Document the assessment
 Agree it with your supervisor
 Use those control measures
You will receive specific training on how to do this
in your department
Control Measures (in order of preference)
1. Use a less risky
substance
2. Use a safer form of
that substance (eg
solution instead of
powder)
Control Measures (in order of preference)
3. Totally enclose the
process (eg a glove-
box)
4. Partially enclose the
process (eg with a
fume cupboard)
5. Ensure good general
ventilation
Control Measures (in order of preference)
6. Safe systems of work
7. Reduce exposure
times, increase
distance, reduce
volumes
8. Personal protective
equipment (as a last
resort for primary protection)
Protecting yourself
 Wear the clothing and
protective wear identified
in your risk assessment
 Laboratory coats must be
kept fastened
 Don’t wear sandals or
open shoes
 Long hair must be tied
back
Protecting yourself - gloves
 There are many different types
of protective glove
 Use the correct ones for the
job you will be doing
 Remember that you need to
select chemical protection
gloves according to the
materials and/or substances
with which you will be working
 Remove your gloves before
using instruments, telephone,
and leaving the laboratory
Laboratory hygiene
 Never eat, drink or smoke
in a laboratory
 Never apply cosmetics
 Never touch your face,
mouth or eyes
 Never suck pens or chew
pencils
 Always wash your hands
before you leave and
especially before eating
What are the general hazards in a
laboratory?
 Fire
 Breakage of glassware
 Sharps
 Pressure equipment & gas
cylinders
 Extremes of heat & cold
 Chemical hazards
 Biological hazards
 Radiation
And many more!
Avoiding Fires
 Flammable substances
 Use minimum quantity
 Store in special storage
cabinet
 Use temperature-
controlled heating
sources
(eg water-bath rather than
hot-plate or Bunsen
burner)
Minimise fire damage
 Make sure corridor
fire doors and
laboratory doors are
kept shut at all times
Fire Safety
 Make sure that you know
what to do:
 If you have a fire
 If you hear a fire alarm
 If you are a member of
staff you must attend fire
training annually. Post
graduates should also
seriously consider doing
so.
Glassware
 Use correct techniques for the
insertion of tubing onto glassware
 Never use glassware under
pressure or vacuum unless it is
designed for the job and suitably
shielded
 Dispose of chipped or broken
glassware – it is a risk to you and
others
 Always dispose of broken glass in
a glass bin or sharps bin and not in
a general waste bin
Spillages
 Clear up spillage promptly
 You will already have
determined how to do this
as part of your risk
assessment
 Dispose of any hazardous
material as toxic waste
Messy workers are usually
poor workers!!
Gas cylinders
 Never use without formal training
 Minimise the number in a laboratory
 Store externally whenever possible
 Cylinders are heavy and can do serious
damage to you if they fall
 Ensure that they are chained when in use
 Move only with a cylinder trolley
 Use regulators & control equipment
suitable for the gas concerned
 Consider the consequences if your
cylinder leaks
Cryogenics
 Liquid gasses are extremely
cold and can cause burns
 Liquid gases evaporate and
many can cause asphyxiation
 If you need to take cryogens
in a lift, there are special
procedures to follow – speak
to your supervisor or a senior
member of technical staff
 You must have special
training to use them
Electrical Equipment
 Always do a visual check
on electrical equipment
before use, looking for
obvious wear or defects
 All portable electrical
equipment must have a
current “PAT test” sticker
 NEVER use defective
equipment
General Tidiness
 Keep your workplace tidy
 Clear up waste, deal with
washing up and put
things away as you finish
with them
 Make sure everything is
safe before you leave
things unattended
 A tidy laboratory avoids
accidents to everyone
X
Laboratory Equipment
 Never use any
laboratory equipment
unless you are trained
& have been
authorised to do so
 As well as injuring
yourself you may
cause very costly
damage
First Aid
 All laboratory workers
should undergo simple first
aid training
 For ALL chemical splashes,
wash with plenty of water for
10 minutes
 Control bleeding with direct
pressure, avoiding any foreign
bodies such as glass
 Report all accidents to your
supervisor or departmental
safety officer
Protecting your health
 If you have an allergy
to lab materials or
suffer from a medical
condition which may
affect you in the
laboratory (eg
diabetes or epilepsy),
ensure that your
supervisor knows
Waste Materials
 Part of your risk assessment
will be to determine how to
dispose of waste lab materials
safely
 Solvents and oils must be
segregated into the correct
waste bottle or drum
 Your department will help you
determine what to do with
chemical or biological materials
 Do not put materials down the
drain or in with normal waste
unless authorised to do so
Working outside normal hours and
at weekends
 You will need to attend
training courses and have
permission from your
Head of Department
before working outside
normal hours
 Most experimental work
is not permitted
 Your supervisor will
explain the requirements
in more detail
When in doubt – ASK!!!
 Do not carry out a
new or unfamiliar
procedure until you
have been fully
trained & understand
the precautions
necessary for safe
working
 DO NOT GUESS!!!!

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Labsafety and theic that help to have a good lab environment

  • 2. Why does it matter?  Safe working protects:  You  Other lab workers  Cleaners  Visitors  Your work
  • 3. What does the law say? (1)  Health Safety at Work etc Act 1974  You must work safely  You must not endanger others  You must not misuse safety equipment  Penalty – up to 2 year in prison &/or an unlimited fine
  • 4. What does the law say? (2)  The Management of Health and Safety at Work Regs 1999  Control of Substances Hazardous to Health Regs 2004  You must perform RISK ASSESSMENTS
  • 5. How to do a Risk Assessment?  Determine hazards and evaluate risks  Use all relevant available data  Determine controls needed to minimise those risks  Document the assessment  Agree it with your supervisor  Use those control measures You will receive specific training on how to do this in your department
  • 6. Control Measures (in order of preference) 1. Use a less risky substance 2. Use a safer form of that substance (eg solution instead of powder)
  • 7. Control Measures (in order of preference) 3. Totally enclose the process (eg a glove- box) 4. Partially enclose the process (eg with a fume cupboard) 5. Ensure good general ventilation
  • 8. Control Measures (in order of preference) 6. Safe systems of work 7. Reduce exposure times, increase distance, reduce volumes 8. Personal protective equipment (as a last resort for primary protection)
  • 9. Protecting yourself  Wear the clothing and protective wear identified in your risk assessment  Laboratory coats must be kept fastened  Don’t wear sandals or open shoes  Long hair must be tied back
  • 10. Protecting yourself - gloves  There are many different types of protective glove  Use the correct ones for the job you will be doing  Remember that you need to select chemical protection gloves according to the materials and/or substances with which you will be working  Remove your gloves before using instruments, telephone, and leaving the laboratory
  • 11. Laboratory hygiene  Never eat, drink or smoke in a laboratory  Never apply cosmetics  Never touch your face, mouth or eyes  Never suck pens or chew pencils  Always wash your hands before you leave and especially before eating
  • 12. What are the general hazards in a laboratory?  Fire  Breakage of glassware  Sharps  Pressure equipment & gas cylinders  Extremes of heat & cold  Chemical hazards  Biological hazards  Radiation And many more!
  • 13. Avoiding Fires  Flammable substances  Use minimum quantity  Store in special storage cabinet  Use temperature- controlled heating sources (eg water-bath rather than hot-plate or Bunsen burner)
  • 14. Minimise fire damage  Make sure corridor fire doors and laboratory doors are kept shut at all times
  • 15. Fire Safety  Make sure that you know what to do:  If you have a fire  If you hear a fire alarm  If you are a member of staff you must attend fire training annually. Post graduates should also seriously consider doing so.
  • 16. Glassware  Use correct techniques for the insertion of tubing onto glassware  Never use glassware under pressure or vacuum unless it is designed for the job and suitably shielded  Dispose of chipped or broken glassware – it is a risk to you and others  Always dispose of broken glass in a glass bin or sharps bin and not in a general waste bin
  • 17. Spillages  Clear up spillage promptly  You will already have determined how to do this as part of your risk assessment  Dispose of any hazardous material as toxic waste Messy workers are usually poor workers!!
  • 18. Gas cylinders  Never use without formal training  Minimise the number in a laboratory  Store externally whenever possible  Cylinders are heavy and can do serious damage to you if they fall  Ensure that they are chained when in use  Move only with a cylinder trolley  Use regulators & control equipment suitable for the gas concerned  Consider the consequences if your cylinder leaks
  • 19. Cryogenics  Liquid gasses are extremely cold and can cause burns  Liquid gases evaporate and many can cause asphyxiation  If you need to take cryogens in a lift, there are special procedures to follow – speak to your supervisor or a senior member of technical staff  You must have special training to use them
  • 20. Electrical Equipment  Always do a visual check on electrical equipment before use, looking for obvious wear or defects  All portable electrical equipment must have a current “PAT test” sticker  NEVER use defective equipment
  • 21. General Tidiness  Keep your workplace tidy  Clear up waste, deal with washing up and put things away as you finish with them  Make sure everything is safe before you leave things unattended  A tidy laboratory avoids accidents to everyone X
  • 22. Laboratory Equipment  Never use any laboratory equipment unless you are trained & have been authorised to do so  As well as injuring yourself you may cause very costly damage
  • 23. First Aid  All laboratory workers should undergo simple first aid training  For ALL chemical splashes, wash with plenty of water for 10 minutes  Control bleeding with direct pressure, avoiding any foreign bodies such as glass  Report all accidents to your supervisor or departmental safety officer
  • 24. Protecting your health  If you have an allergy to lab materials or suffer from a medical condition which may affect you in the laboratory (eg diabetes or epilepsy), ensure that your supervisor knows
  • 25. Waste Materials  Part of your risk assessment will be to determine how to dispose of waste lab materials safely  Solvents and oils must be segregated into the correct waste bottle or drum  Your department will help you determine what to do with chemical or biological materials  Do not put materials down the drain or in with normal waste unless authorised to do so
  • 26. Working outside normal hours and at weekends  You will need to attend training courses and have permission from your Head of Department before working outside normal hours  Most experimental work is not permitted  Your supervisor will explain the requirements in more detail
  • 27. When in doubt – ASK!!!  Do not carry out a new or unfamiliar procedure until you have been fully trained & understand the precautions necessary for safe working  DO NOT GUESS!!!!