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Drought-induced Changes in Root and Shoot Growth of Pigeonpea
Vol. 33, No. 4, October-December 2015 2815
*
C.P. College of Agriculture, Sardar Krushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat - 385506,
India.
I J T A© Serials Publications
National Academy of Agricultural Science (NAAS) Rating : 3. 03
Drought-induced ChangesinRootandShootGrowthofPigeonpea[Cajanuscajan
(L.) Millspaugh]
K. Suresh*
, H.S. Bhadauria, Y. Ravindra babu and K. Satish, Satyendra Singh
Abstract: Twenty pigeonpea genotypes were sowed separately under rainfed and irrigated condition for evaluating root
traits associated to drought tolerance in kharif 2013. The genotype SKNP 1004 was marked by maximum root length, root
dry weight and seed yield in both rainfed and irrigated conditions and it showed highest shoot length in rainfed condition.
The genotype SKNP 1005 recorded utmost values for shoot length and shoot dry weight in both conditions. The root/shoot
ratio were superior for UPAS 120 in rainfed condition while it was highest for SKNP 1003 in irrigated condition. Therefore,
SKNP 1004 and SKNP 1005 were rated as most promising genotype for drought tolerance.
Keywords: Pigeonpea, Root length, Shoot length, Root/Shoot ratio.
INTRODUCTION
Pigeonpea [Cajanus cajan (L.) Millspaugh] is also
popularly known as Redgram, Tur and Arhar. It is
one of the major grain legume crops of the tropics
and subtropics grown approximately 50 countries
which spread in continents of Asia, Africa and
America. Drought stress, characterized by low
availability of soil moisture and high evapo-
transpiration is considered one of the principal causes
which decrease crop productivity worldwide Boyer
(1982). Pigeonpea is normally cultivated as a rainy
crop and is often subjected to water stress at one or
several stages of crop growth and development,
since it is a long duration crop, drought stress is
considered as a severe threat for sustainable crop
production in the conditions of climate change.
Accordingly, the present study was planned to find
out a simple and precise field method to detect
genotypic differences in drought tolerance and to
quantify loss in yield.
MATERIAL AND METHODS
Twenty genotypes were evaluated in rainfed and
irrigated conditions with three replications and
maintaining 60 cm (Row to Row) 20 cm (Plant to
Plant) spacing. The crop in rainfed condition was
sowed just after monsoon. Three irrigations applied
in irrigated set after ceasation of monsoon. The crop
was raised in protective root structure which is 6 ft.
high from the ground level and it was filled with
uniform soil. The roots were extracted carefully by
removing soil with the help of water. The root and
shoot length were measured for five randomly
tagged plants at 60 days after sowing and calculated
by the mean of five plants. After harvesting, the fresh
roots and shoots were sun dried then oven dried
for 48 hrs at 60°C, root and shoot dry weight recorded
with help of balance and expressed as g/plant. The
root dry weight was divided with shoot dry weight
for five randomly tagged plants at 60 days after
sowing and root shoot ratio calculated by the mean
of five plants. The recommended package of
practices were adopted during crop period.
RESULTS AND DISCUSSION
Identification of a drought tolerant variety of
pigeonpea is a difficult task for several reasons
because so many attributes are related to drought
tolerance. It is highly impossible to have a genotype
possessing all these attributes responsible for
drought tolerance. For the selection of such
genotypes, the study on root traits related to plant
K. Suresh, H.S. Bhadauria, Y. Ravindra Babu, K. Satish and Satyendra Singh
2816 International Journal of Tropical Agriculture © Serials Publications, ISSN: 0254-8755
Table1
Drymatterproductionandtheirdistributionincomponentpartsofplantunderrainfedandirrigatedconditions(g/plant).
GenotypesRootlengthShootlengthRootdryweightShootdryweightRoot/ShootratioSeedyield
(cm)(cm)(g/plant)(g/plant)(g/plant)
I0
I1
I0
I1
I0
I0
I0
I1
I0
I1
I0
I1
GT10152.9338.0036.2754.930.460.260.882.120.520.1223.0259.21
UPAS12041.5334.4024.9348.200.440.190.511.650.870.1115.9649.29
SKNP061546.9334.8732.3343.330.280.160.551.610.520.1019.2146.66
SKNP061648.8725.6725.4043.730.300.230.901.590.340.1517.5853.28
SKNP080553.8036.3336.3356.830.510.290.892.240.570.1324.7461.87
SKNP090445.0728.4326.1345.200.270.190.561.410.480.1318.7746.50
SKNP090535.3033.6733.0053.400.300.170.662.010.460.0919.5551.84
SKNP092047.8734.9033.4745.870.320.180.691.390.460.1317.1650.25
SKNP100145.2732.3332.9348.670.340.200.621.550.560.1318.0445.94
SKNP100334.6023.6724.9343.270.220.200.841.130.260.1814.2538.22
SKNP100457.4747.8738.8057.200.560.380.932.280.610.1729.8165.11
SKNP100556.2044.4737.9058.330.520.340.952.350.550.1528.7161.28
SKNP100642.3324.4729.1350.900.370.180.441.840.840.1017.6446.62
SKNP121143.0726.3730.8051.730.270.210.631.870.430.1116.5949.27
SKNP121640.5324.6730.2751.670.260.180.711.860.370.1018.2547.67
SKNP121740.0724.4725.5348.070.280.200.561.480.540.1419.1950.65
SKNP110953.0735.8735.2855.070.410.210.852.050.490.1021.8852.11
SKNP100836.4025.2728.9346.200.260.230.661.460.400.1617.5646.43
SKNP102152.8036.6734.3354.000.450.250.802.110.560.1222.6960.76
BANAS52.4035.7334.2753.530.440.230.581.990.750.1123.3056.35
S.Em±2.431.541.702.030.020.010.040.090.040.010.982.37
CDat5%6.964.414.875.800.050.040.120.260.130.032.796.79
CV%9.108.249.356.957.8810.4010.48.8314.412.48.377.92
I0
:Rainfedcondition,I1
:Irrigatedcondition,DTE:Droughttoleranceefficiency,DSI:Droughtsusceptibilityindex.
Figure 1: Effect of rainfed and irrigated conditions on root
length at 60 days after sowing
Figure 2: Effect of rainfed and irrigated conditions on shoot
length at 60 days after sowing
parts is essential. Twenty pigeonpea genotypes were
evaluated for drought tolerance with the attributes
like root length, shoot length, root dry weight, shoot
dry weight, root shoot ratio and seed yield.
The perusal of the table 1, which represent data
of rainfed and irrigated conditions revealed that,
the genotype SKNP 1004 was marked by maximum
root length. Root elongation during drought help
plants to get deeper water, thus avoiding water
deficits near the soil surface. Deeper root penetration
is potentially an important component of drought
resistance, greater proportion of the plant’s
assimilates can be distributed to the root system,
where they can support further root growth.
Enhanced root growth into moist soil zones during
stress requires allocation of assimilates to the
growing root tips. Similar results of increase in root
length under moisture stress were recorded by
Hossain et al. (2014).
In rainfed condition the genotype SKNP 1004
maintained significantly higher shoot length while
in case of irrigated condition SKNP 1005 obtained
maximum shoot length. The reduction in shoot
growth under induced drought stress originates not
only from growth inhibitions but also from a loss of
Drought-induced Changes in Root and Shoot Growth of Pigeonpea
Vol. 33, No. 4, October-December 2015 2817
turgidity. The translocation of assimilates towards
the roots due to decreases in shoot growth. Similar
results of reduced shoot length were also recorded
by Kibreab et al. (2013).
In both rainfed and irrigated conditions the
genotype SKNP 1004 showed superiority for the
value of root dry weights. Plant productivity under
drought stress is strongly related to the processes
of dry matter partitioning and biomass distribution.
Elongation of the taproots in the rainfed plants
occurred at the expense of thickening. These results
were also supported by Kataria and Singh (2014).
The genotype SKNP 1005 recorded maximum
shoot dry weight in rainfed and irrigated over the
rest of other genotypes. The results showed that
water stress reduced shoot dry weight even partial
dried root systems can lead to decreased allocation
of photosynthates to vegetative shoots. Longer roots
may reduce shoot dry weight by allowing the
partitioning of photosynthate towards roots at the
expense of shoots. Water stress lowers the cell turgor
and causes slower cell expansion, consequently
growth and development of a plant decreased that
leads to a lower shoot dry weights. The results of
the present study is similar to the findings of Amira
and Qados (2014).
Figure 3: Effect of rainfed and irrigated conditions on root
dry weight at 60 days after sowing
Figure 4: Effect of rainfed and irrigated conditions on shoot
dry weight at 60 days after sowing
Figure 5: Effect on root/shoot ratio of rainfed and irrigated
conditions at 60 days after sowing.
Figure 6: Effect of rainfed and irrigated conditions on seed
yield per plant
The rainfed data showed that utmost root/shoot
ratio was observed by the genotype UPAS 120, while
in irrigated condition the genotype SKNP 1003 had
most prominent root shoot ratio across the left over
genotypes. Plants in dry conditions often decrease
biomass production and contribute more biomass to
roots, maintaining a higher root shoot ratio as an
adaptation trait to drought resistance. High root/
shoot ratio was found to increase water uptake and
have a positive effect on yield under stress. Similar
results of increased root shoot ratio were recorded
by Uddin et al. (2013).
SKNP 1004 is characterized by highest seed yield
under rainfed as well as irrigated conditions.
Drought inhibited growth by reducing rate of cell
division and cell expansion, leaf size, stem elongation
as well as root proliferation, by disturbing stomatal
oscillations, metabolic activities, plant water and
nutrient relations, loss of pollen and stigma viability
which affects yield components and all together
decreases seed yield. Moisture stress reduced yield
due to poor partitioning operated along with terminal
drought stress. The present study was supported
by Ahmed and suliman (2010).
K. Suresh, H.S. Bhadauria, Y. Ravindra Babu, K. Satish and Satyendra Singh
2818 International Journal of Tropical Agriculture © Serials Publications, ISSN: 0254-8755
SUMMARY AND CONCLUSION
It is concluded from results that the genotype SKNP
1004 was found to be a boon for drought tolerance
followed by SKNP 1005 under rainfed as well as
irrigated conditions. Therefore on the basis of these
observations, SKNP 1004was rated as most promising
genotype due to highest seed yield under rainfed as
well as irrigated condition. SKNP 1005 was another
promising genotype for yield under rainfed and
irrigated condition.
REFERENCES
Ahmed, F.E. and Suliman, A.S.H. (2010), Effect of water
stress applied at different stages of growth on seed
yield and water-use efficiency of Cowpea. Agriculture
and Biology Journal of North America. 1(4): 534-540.
Amira, M. S. and Qados, A. (2014), Effect of Ascorbic Acid
antioxidant on Soybean (Glycine max L.) plants grown
under water stress conditions. International Journal on
Advanced Research in Biological Science. 1(6): 189-205
Boyer, J.S. (1982), Plant productivity and environment.
Science. 218: 443-448.
Hossain, M.A.; Mostofa, M.G.; Burritt, D.J. and Fujita, M.
(2014), Modulation of Reactive Oxygen Species and
Methylglyoxal Detoxification Systems by Exogenous
Glycinebetaine and Proline Improves Drought
Tolerance in Mustard (Brassica juncea L.). International
Journal of Plant Biology & Research. 2(2): 1014-1017.
Kibreab, M.; Ghebremariam, Liang Y.; Zhengcai, Z. and
Qiaoli, W. (2013), Effect of drought stress on
physiological growth parameters of tomato inbred
lines at germination stage. European Scientific Journal.
9: 33.
Kataria, N. and Singh, N. (2014), Assesing the effects of
aplied potassium on selected Vigna radiata L.
genotypes under water deficict. International Journal
of Advanced Research 2(10): 369-385.
Uddin, S.; Parwin, S. and Awal, M.A. (2013),
Morphophysiological aspects of mungbean (Vigna
radiata L.) in response to water stress.
���

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K. SURESH

  • 1. Drought-induced Changes in Root and Shoot Growth of Pigeonpea Vol. 33, No. 4, October-December 2015 2815 * C.P. College of Agriculture, Sardar Krushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat - 385506, India. I J T A© Serials Publications National Academy of Agricultural Science (NAAS) Rating : 3. 03 Drought-induced ChangesinRootandShootGrowthofPigeonpea[Cajanuscajan (L.) Millspaugh] K. Suresh* , H.S. Bhadauria, Y. Ravindra babu and K. Satish, Satyendra Singh Abstract: Twenty pigeonpea genotypes were sowed separately under rainfed and irrigated condition for evaluating root traits associated to drought tolerance in kharif 2013. The genotype SKNP 1004 was marked by maximum root length, root dry weight and seed yield in both rainfed and irrigated conditions and it showed highest shoot length in rainfed condition. The genotype SKNP 1005 recorded utmost values for shoot length and shoot dry weight in both conditions. The root/shoot ratio were superior for UPAS 120 in rainfed condition while it was highest for SKNP 1003 in irrigated condition. Therefore, SKNP 1004 and SKNP 1005 were rated as most promising genotype for drought tolerance. Keywords: Pigeonpea, Root length, Shoot length, Root/Shoot ratio. INTRODUCTION Pigeonpea [Cajanus cajan (L.) Millspaugh] is also popularly known as Redgram, Tur and Arhar. It is one of the major grain legume crops of the tropics and subtropics grown approximately 50 countries which spread in continents of Asia, Africa and America. Drought stress, characterized by low availability of soil moisture and high evapo- transpiration is considered one of the principal causes which decrease crop productivity worldwide Boyer (1982). Pigeonpea is normally cultivated as a rainy crop and is often subjected to water stress at one or several stages of crop growth and development, since it is a long duration crop, drought stress is considered as a severe threat for sustainable crop production in the conditions of climate change. Accordingly, the present study was planned to find out a simple and precise field method to detect genotypic differences in drought tolerance and to quantify loss in yield. MATERIAL AND METHODS Twenty genotypes were evaluated in rainfed and irrigated conditions with three replications and maintaining 60 cm (Row to Row) 20 cm (Plant to Plant) spacing. The crop in rainfed condition was sowed just after monsoon. Three irrigations applied in irrigated set after ceasation of monsoon. The crop was raised in protective root structure which is 6 ft. high from the ground level and it was filled with uniform soil. The roots were extracted carefully by removing soil with the help of water. The root and shoot length were measured for five randomly tagged plants at 60 days after sowing and calculated by the mean of five plants. After harvesting, the fresh roots and shoots were sun dried then oven dried for 48 hrs at 60°C, root and shoot dry weight recorded with help of balance and expressed as g/plant. The root dry weight was divided with shoot dry weight for five randomly tagged plants at 60 days after sowing and root shoot ratio calculated by the mean of five plants. The recommended package of practices were adopted during crop period. RESULTS AND DISCUSSION Identification of a drought tolerant variety of pigeonpea is a difficult task for several reasons because so many attributes are related to drought tolerance. It is highly impossible to have a genotype possessing all these attributes responsible for drought tolerance. For the selection of such genotypes, the study on root traits related to plant
  • 2. K. Suresh, H.S. Bhadauria, Y. Ravindra Babu, K. Satish and Satyendra Singh 2816 International Journal of Tropical Agriculture © Serials Publications, ISSN: 0254-8755 Table1 Drymatterproductionandtheirdistributionincomponentpartsofplantunderrainfedandirrigatedconditions(g/plant). GenotypesRootlengthShootlengthRootdryweightShootdryweightRoot/ShootratioSeedyield (cm)(cm)(g/plant)(g/plant)(g/plant) I0 I1 I0 I1 I0 I0 I0 I1 I0 I1 I0 I1 GT10152.9338.0036.2754.930.460.260.882.120.520.1223.0259.21 UPAS12041.5334.4024.9348.200.440.190.511.650.870.1115.9649.29 SKNP061546.9334.8732.3343.330.280.160.551.610.520.1019.2146.66 SKNP061648.8725.6725.4043.730.300.230.901.590.340.1517.5853.28 SKNP080553.8036.3336.3356.830.510.290.892.240.570.1324.7461.87 SKNP090445.0728.4326.1345.200.270.190.561.410.480.1318.7746.50 SKNP090535.3033.6733.0053.400.300.170.662.010.460.0919.5551.84 SKNP092047.8734.9033.4745.870.320.180.691.390.460.1317.1650.25 SKNP100145.2732.3332.9348.670.340.200.621.550.560.1318.0445.94 SKNP100334.6023.6724.9343.270.220.200.841.130.260.1814.2538.22 SKNP100457.4747.8738.8057.200.560.380.932.280.610.1729.8165.11 SKNP100556.2044.4737.9058.330.520.340.952.350.550.1528.7161.28 SKNP100642.3324.4729.1350.900.370.180.441.840.840.1017.6446.62 SKNP121143.0726.3730.8051.730.270.210.631.870.430.1116.5949.27 SKNP121640.5324.6730.2751.670.260.180.711.860.370.1018.2547.67 SKNP121740.0724.4725.5348.070.280.200.561.480.540.1419.1950.65 SKNP110953.0735.8735.2855.070.410.210.852.050.490.1021.8852.11 SKNP100836.4025.2728.9346.200.260.230.661.460.400.1617.5646.43 SKNP102152.8036.6734.3354.000.450.250.802.110.560.1222.6960.76 BANAS52.4035.7334.2753.530.440.230.581.990.750.1123.3056.35 S.Em±2.431.541.702.030.020.010.040.090.040.010.982.37 CDat5%6.964.414.875.800.050.040.120.260.130.032.796.79 CV%9.108.249.356.957.8810.4010.48.8314.412.48.377.92 I0 :Rainfedcondition,I1 :Irrigatedcondition,DTE:Droughttoleranceefficiency,DSI:Droughtsusceptibilityindex. Figure 1: Effect of rainfed and irrigated conditions on root length at 60 days after sowing Figure 2: Effect of rainfed and irrigated conditions on shoot length at 60 days after sowing parts is essential. Twenty pigeonpea genotypes were evaluated for drought tolerance with the attributes like root length, shoot length, root dry weight, shoot dry weight, root shoot ratio and seed yield. The perusal of the table 1, which represent data of rainfed and irrigated conditions revealed that, the genotype SKNP 1004 was marked by maximum root length. Root elongation during drought help plants to get deeper water, thus avoiding water deficits near the soil surface. Deeper root penetration is potentially an important component of drought resistance, greater proportion of the plant’s assimilates can be distributed to the root system, where they can support further root growth. Enhanced root growth into moist soil zones during stress requires allocation of assimilates to the growing root tips. Similar results of increase in root length under moisture stress were recorded by Hossain et al. (2014). In rainfed condition the genotype SKNP 1004 maintained significantly higher shoot length while in case of irrigated condition SKNP 1005 obtained maximum shoot length. The reduction in shoot growth under induced drought stress originates not only from growth inhibitions but also from a loss of
  • 3. Drought-induced Changes in Root and Shoot Growth of Pigeonpea Vol. 33, No. 4, October-December 2015 2817 turgidity. The translocation of assimilates towards the roots due to decreases in shoot growth. Similar results of reduced shoot length were also recorded by Kibreab et al. (2013). In both rainfed and irrigated conditions the genotype SKNP 1004 showed superiority for the value of root dry weights. Plant productivity under drought stress is strongly related to the processes of dry matter partitioning and biomass distribution. Elongation of the taproots in the rainfed plants occurred at the expense of thickening. These results were also supported by Kataria and Singh (2014). The genotype SKNP 1005 recorded maximum shoot dry weight in rainfed and irrigated over the rest of other genotypes. The results showed that water stress reduced shoot dry weight even partial dried root systems can lead to decreased allocation of photosynthates to vegetative shoots. Longer roots may reduce shoot dry weight by allowing the partitioning of photosynthate towards roots at the expense of shoots. Water stress lowers the cell turgor and causes slower cell expansion, consequently growth and development of a plant decreased that leads to a lower shoot dry weights. The results of the present study is similar to the findings of Amira and Qados (2014). Figure 3: Effect of rainfed and irrigated conditions on root dry weight at 60 days after sowing Figure 4: Effect of rainfed and irrigated conditions on shoot dry weight at 60 days after sowing Figure 5: Effect on root/shoot ratio of rainfed and irrigated conditions at 60 days after sowing. Figure 6: Effect of rainfed and irrigated conditions on seed yield per plant The rainfed data showed that utmost root/shoot ratio was observed by the genotype UPAS 120, while in irrigated condition the genotype SKNP 1003 had most prominent root shoot ratio across the left over genotypes. Plants in dry conditions often decrease biomass production and contribute more biomass to roots, maintaining a higher root shoot ratio as an adaptation trait to drought resistance. High root/ shoot ratio was found to increase water uptake and have a positive effect on yield under stress. Similar results of increased root shoot ratio were recorded by Uddin et al. (2013). SKNP 1004 is characterized by highest seed yield under rainfed as well as irrigated conditions. Drought inhibited growth by reducing rate of cell division and cell expansion, leaf size, stem elongation as well as root proliferation, by disturbing stomatal oscillations, metabolic activities, plant water and nutrient relations, loss of pollen and stigma viability which affects yield components and all together decreases seed yield. Moisture stress reduced yield due to poor partitioning operated along with terminal drought stress. The present study was supported by Ahmed and suliman (2010).
  • 4. K. Suresh, H.S. Bhadauria, Y. Ravindra Babu, K. Satish and Satyendra Singh 2818 International Journal of Tropical Agriculture © Serials Publications, ISSN: 0254-8755 SUMMARY AND CONCLUSION It is concluded from results that the genotype SKNP 1004 was found to be a boon for drought tolerance followed by SKNP 1005 under rainfed as well as irrigated conditions. Therefore on the basis of these observations, SKNP 1004was rated as most promising genotype due to highest seed yield under rainfed as well as irrigated condition. SKNP 1005 was another promising genotype for yield under rainfed and irrigated condition. REFERENCES Ahmed, F.E. and Suliman, A.S.H. (2010), Effect of water stress applied at different stages of growth on seed yield and water-use efficiency of Cowpea. Agriculture and Biology Journal of North America. 1(4): 534-540. Amira, M. S. and Qados, A. (2014), Effect of Ascorbic Acid antioxidant on Soybean (Glycine max L.) plants grown under water stress conditions. International Journal on Advanced Research in Biological Science. 1(6): 189-205 Boyer, J.S. (1982), Plant productivity and environment. Science. 218: 443-448. Hossain, M.A.; Mostofa, M.G.; Burritt, D.J. and Fujita, M. (2014), Modulation of Reactive Oxygen Species and Methylglyoxal Detoxification Systems by Exogenous Glycinebetaine and Proline Improves Drought Tolerance in Mustard (Brassica juncea L.). International Journal of Plant Biology & Research. 2(2): 1014-1017. Kibreab, M.; Ghebremariam, Liang Y.; Zhengcai, Z. and Qiaoli, W. (2013), Effect of drought stress on physiological growth parameters of tomato inbred lines at germination stage. European Scientific Journal. 9: 33. Kataria, N. and Singh, N. (2014), Assesing the effects of aplied potassium on selected Vigna radiata L. genotypes under water deficict. International Journal of Advanced Research 2(10): 369-385. Uddin, S.; Parwin, S. and Awal, M.A. (2013), Morphophysiological aspects of mungbean (Vigna radiata L.) in response to water stress. ���