This document provides a summary of Johnathan Broady's qualifications including his education, awards, technical experience, publications, memberships, and teaching experience. He holds an M.S. in Animal Sciences from Auburn University and a B.S. in Biology from Washington State University. Currently he works as the Food Safety Superintendent at JBS Souderton plant where he oversees food safety audits and liaises with the USDA. He has extensive experience in food safety and microbiology research.
Molecular Biologist Academic CV for Industry or Private Sector Consideration Sirie Godshalk
Molecular Biologist with over thirteen years of hands-on research experience, impactful writer and presenter, dynamic leader and enthusiastic team player with an eye for great ideas and a passion to move science in new directions seeks challenging opportunities beyond the bench.
Molecular Biologist Academic CV for Industry or Private Sector Consideration Sirie Godshalk
Molecular Biologist with over thirteen years of hands-on research experience, impactful writer and presenter, dynamic leader and enthusiastic team player with an eye for great ideas and a passion to move science in new directions seeks challenging opportunities beyond the bench.
Gabriel Fenteany's curriculum vitae. I have extensive experience in research and teaching, having worked as a tenured professor at a major research university. My particular areas of focus entailed chemical biology, organic chemistry, biochemistry, and molecular and cell biology. I have developed and curate a number of online resources in chemistry and biology.
Identification of the positively selected genes governing host-pathogen arm r...Atai Rabby
Bacterial evolution is due to the adaptive nature of the core bacterial genomes that plays critical role in diversification, fitness and adaptation of the species to different environment and host. Since Vibrio cholerae represents an appropriate model organism for studying the interplay of environment and host driven factors shaping the microbial genome structure and function, the current study aims to identify genes that are under these strong forces in V. cholerae. Here, we employed a comparative genomics approach to identify genes that are under positive selection in ten strains of Vibrio sp. including four pathogenic V. cholerae strains. From the available genome sequence data, a total of 422 orthologous genes were identified by reciprocal BLAST best-hit method, recombination breakpoint frequency analysis and tree comparison method. These 422 genes, representing the core genome of Vibrio sp., constituted the dataset to be analyzed for evolutionary selections. The analysis of natural selection, based on Maximum Likelihood method on synonymous and non-synonymous substitution rate, confirms the hypothesis that the bacterial core genomes are mostly under purifying selection with a few positively selected regions. However, our finding also reveals that positively selected sites in the Vibrio genome occur in a wide range of different genes encompassing diverse functional pathways including cell surface proteins (e.g. outer membrane-specific lipoprotein transporter/assembly proteins etc.), cell motility proteins (e.g. flagellar motor switch proteins, flagellar hook and assembly proteins), nutrient acquisition (e.g. amino acid, carbohydrate and phosphate ABC transporters), DNA repair and transcription related proteins. Interestingly, these positively selected gene products are directly involved with host-pathogen interactions and fitness in gastrointestinal environment. Therefore, the collective evidences of these positively selected genes spanning several pathways raise the possibility of their involvement in evolutionary arms races with other bacteria, phages, and/or the host immune system. This finding points to the natural selections which is the responsible factor for the diversification of Vibrio genus.
Gabriel Fenteany's curriculum vitae. I have extensive experience in research and teaching, having worked as a tenured professor at a major research university. My particular areas of focus entailed chemical biology, organic chemistry, biochemistry, and molecular and cell biology. I have developed and curate a number of online resources in chemistry and biology.
Identification of the positively selected genes governing host-pathogen arm r...Atai Rabby
Bacterial evolution is due to the adaptive nature of the core bacterial genomes that plays critical role in diversification, fitness and adaptation of the species to different environment and host. Since Vibrio cholerae represents an appropriate model organism for studying the interplay of environment and host driven factors shaping the microbial genome structure and function, the current study aims to identify genes that are under these strong forces in V. cholerae. Here, we employed a comparative genomics approach to identify genes that are under positive selection in ten strains of Vibrio sp. including four pathogenic V. cholerae strains. From the available genome sequence data, a total of 422 orthologous genes were identified by reciprocal BLAST best-hit method, recombination breakpoint frequency analysis and tree comparison method. These 422 genes, representing the core genome of Vibrio sp., constituted the dataset to be analyzed for evolutionary selections. The analysis of natural selection, based on Maximum Likelihood method on synonymous and non-synonymous substitution rate, confirms the hypothesis that the bacterial core genomes are mostly under purifying selection with a few positively selected regions. However, our finding also reveals that positively selected sites in the Vibrio genome occur in a wide range of different genes encompassing diverse functional pathways including cell surface proteins (e.g. outer membrane-specific lipoprotein transporter/assembly proteins etc.), cell motility proteins (e.g. flagellar motor switch proteins, flagellar hook and assembly proteins), nutrient acquisition (e.g. amino acid, carbohydrate and phosphate ABC transporters), DNA repair and transcription related proteins. Interestingly, these positively selected gene products are directly involved with host-pathogen interactions and fitness in gastrointestinal environment. Therefore, the collective evidences of these positively selected genes spanning several pathways raise the possibility of their involvement in evolutionary arms races with other bacteria, phages, and/or the host immune system. This finding points to the natural selections which is the responsible factor for the diversification of Vibrio genus.
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1. Johnathan W. Broady II
2058 Maple Ave APT#F2-6
Hatfield PA, 19440
johnathanbroady@gmail.com
334-332-4498
EDUCATION
M.S. Animal Sciences, Auburn University
B.S. Biology, Washington State University
RECENT AWARDS & CERTIFICATION
JBS Premier Food Safety Quarterly Award – April 2016
PAACO certification – January 2016
A Grade - British Retail Consortium (BRC) Food Safety Standard 7 – September 2015
2nd
place- AMSA Graduate Student Poster Competition- Summer 2013
Lalor Foundation Award recipient– Summer 2011
Burroughs Welcome Fellowship recipient– Summer 2011
Fred. W. Frasier Memorial Scholarship recipient– Spring 2010
TECHNICAL EXPERIENCE
Food Safety Superintendent – August 2015 to present
Overall authority for food safety at the JBS Souderton plant. Responsible for all food safety audits and
primary liaison to the USDA.
Elliott Keller
Quality Assurance Lab Supervisor – June 2014 to August 2015
Responsibilities included (but not limited to) maintaining the hold and release status of all tested
products at Souderton facility, ensuring effectiveness of food safety interventions on kill floor and
fabrication, and investigating the root cause of presumptives.
Sundeep Yanamala
Ascend Leadership Training Program – June 2014 to June 2015
Developed methods to reduce contamination at the evisceration table.
Sundeep Yanamala
Master’s Student Auburn University – 2012 to 2014
Thesis: Survival of Listeria monocytogenes on Ready-to-Eat Roast Beef During Refrigeration Storage
Dr. Luxin Wang
Technical Assistant I, Washington State University, Pullman Washington - Summer 2012
Examined the prevalence of Escherichia coli populations in local apple orchards.
Dr. Karen Killinger
Research Assistant, Washington State University, Pullman Washington- 2008 to 2012
2. Investigated the role of progesterone receptor membrane component 1 (PGRMC1) on male germ cell
development. Laboratory techniques include cell culture, small animal injections, SSC transplants and
tissue grafting. Journal publication, Dr. Derek McLean
McNair Program Scholar, Washington State University, Pullman Washington Fall 2007- Summer 2008
Investigated the ability of transgenic mice to support subcutaneous spermatogonial stem cell (SSC)
transplants, Dr. Ramon Herrera
Student Research and Apprenticeship Program (SRAP), Internship
Pacific Northwest National Laboratory, Richland Washington, Summer 2003 & 2005
Tested dissolution rates of a borosilicate glass matrix under different pH and temperature treatments to
determine possible vitrification applications for radioactive waste disposal, Poster Presentation, Journal
publication, Dr. Eric Pierce
JOURNAL PUBLICATIONS
Caires K., Broady J., and D. McLean. 2010. Maintaining the male germline: regulation of spermatogonial
stem cells. J Endocrinology 205: 133-145
Pierce E.M., Reed L.R., Shaw W.J., McGrail B.P., Icenhower J.P., Windisch C.F., Cordova E.A., and Broady
J. 2010. Experimental Determination of the Effect of the Ratio of B/Al on Glass Dissolution along the
Nepheline (NaAlSiO4)—Malinkoite (NaBSiO4) Join. Geochimica et Cosmochimica Acta 74(9):2634-2654
ABSTRACT PUBLICATIONS
American Association of Animal Sciences-Southern Section (ASAS), 2014
Microbial diversity of deli roast beef and survival of Listeria monocytogenes
Johnathan Broady 1
, Mark Liles 2
, Christy Bratcher 1
, Elizabeth Schwartz 2
, Luxin Wang 1*
1
Department of Animal Sciences, Auburn University, Auburn, AL 36830; 2
Department of Biological
Sciences, Auburn University, Auburn, AL 36830
American Meat Science Association (AMSA), 2013
Survival and growth of Listeria monocytogenes on deli roast beef during refrigeration storage
Johnathan Broady 1
, Mark Liles 2
, Christy Bratcher 1
, Elizabeth Schwartz 2
, Luxin Wang 1*
1
Department of Animal Sciences, Auburn University, Auburn, AL 36830; 2
Department of Biological
Sciences, Auburn University, Auburn, AL 36830
Society for the Study of Reproduction (SSR), 2012
Conditional deletion of Pgrmc1 in Sertoli cells disrupts germ cell development and
steroidogenesis in the male. Johnathan Broady, Jeanene DeAvila, John Peluso, James Pru, Derek
McLean. Department of Animal Science, Center for Reproductive Biology, Washington State University,
Pullman, WA; Department of Cell Biology, University of Connecticut Health Center, Farmington, CT
Center for Reproductive Biology (CRB), 2010
The Regulation of Spermatogenesis by Membrane-Associated Progesterone Receptor Family Proteins.
Johnathan Broady II, Jeanene DeAvila, and Dr. Derek McLean. Department of Animal Science, Center for
Reproductive Biology, Washington State University, Pullman, WA
3. MEMBERSHIPS
American Society of Animal Sciences 2013- present
International Association for Food Protection 2013- present
American Society for Microbiology 2013- present
American Meat Science Association 2013- present
Society for the Study of Reproduction 2011-2012
Center for Reproductive Biology 2008-2012
TEACHING ASSISTANT EXPERIENCE
Principles of Biochemistry (BCHE3200) - Fall 2013
Animal Growth and Development (ANSC 3610) – Spring 2013
Microbial Meat Safety and Rapid Techniques (ANSC7960) – Fall 2012
General Biology (BIO105)-Spring 2011-Spring 2012
Physiology of Reproduction (AS351)-Spring 2010, Spring 2009
Feeds and Feeding (AS313)-Fall 2010, Fall 2009
Introduction to Animal Science (AS101)-Fall 2008
PROFESSIONAL SERVICE/DEVELOPMENT
Opportunity for Improvement (OFI) Group March 2016 to present
Selected member of leadership group to improve overall slaughter efficiency
Elliott Keller, JBS Souderton
Seminar Committee Fall 2013- present
Coordinated and discussed potential speakers for departmental presentations
Auburn University
Search Committee Member Spring 2011
Candidate search/ranking, interviews/ evaluations, and evaluation/discussion of candidates. Positions
available included a Beef extension and Baxter Endowed Chair. Department of Animal Science,
Washington State University, Pullman, WA
Center for Reproductive Biology Seminar, 2008- 2012
Attended weekly seminars focused on reproductive physiology
UNIV 590 - Preparation for College Teaching course, Fall 2007
Topics include ‘diversity at WSU’, ‘computer application in the classroom’, and ‘helping
students under stress/stress management’
Advanced Student Research and Apprenticeship Program (ASRAP),
Summer 2003 & Summer 2005.
Manipulated abstract dissolution concepts of glass among a team of professional researchers, presented
a poster at Battelle Memorial Institute. Attended several lectures and workshops aimed at professional
development in the scientific field. Pacific Northwest National Laboratory, Richland, WA.
Supervisor: Dr. Eric Pierce
4. GUEST LECTURES
“Nucleic Acid Biotechnology Techniques”. BCHE3200. Course Professors: Dr. Werner Bergen, Dr. Russell
Muntifering, Auburn University. October 2013
“Microbial diversity within RTE foods and the survival of Listeria monocytogenes”. Course Professor:
Dr. Kyle Caires, Berry College. Spring 2014 (invitation)
GRANT DEVELOPMENT
J. Broady “Survival and growth of Listeria monocytogenes on food and food processing equipment”
NOVUS International (2013) – submitted, not awarded
L. Wang, J. Broady “Environmental transfer of Listeria monocytogenes on Alabama farms: connections
between pathogen prevalence and disease transmission” – in development
L. Wang, J. Broady “The effect of packaging systems on pathogen survival in ready to eat (RTE) beef
products” – submitted