1. Harley J. Bobnar
50 South Lincoln Street, Box #74, Washington, PA 15301 • 505 Fourth Avenue New Eagle, PA 15067
724-747-9115 • bobnarhj@jay.washjeff.edu
EDUCATION
Washington & Jefferson College- Washington, PA
Bachelor of Arts in Biochemistry and Minor in English, anticipated May 2017
GPA: 3.197/4.0
RESEARCH EXPERIENCES
Independent Research
Cancer Biology Intern, West Virginia University, Morgantown, WV• May-August 2016
 Investigated the effects of a metastatic osteosarcoma cell line in a mouse model with the
treatment of immunotherapies
 Performed surgeries to inoculate and harvest osteosarcoma tumors
 Utilized IVIS imaging and MRI to track metastasis through mouse’s body
 Performed and Analyzed Flow Cytometry to characterize the tumor’s microenvironment
Course-Based Research
Cell Biology, Washington and Jefferson College, Washington, PA • Fall 2016
 Coinvestigating the ability of natural oils to induce apoptosis in HeLa cells by assaying for cell
viability and using indirect immunofluorescence to detect AIF translocation and phosphorylation
of histone H2B, both indicators of apoptosis.
Molecular Biology, Washington and Jefferson College, Washington, PA • Fall 2016
 Coinvestigating the role of the ATPase domain in Chd1, a chromatin remodeling protein, in a
yeast model system
Introduction to Chemical Research, University of Central Florida, Orlando, FL• January 2015
 Coinvestigated pathogens that grow on different tree barks in the Everglades for bioactivity that
can be utilized in pharmaceuticals
General Biology I, Washington and Jefferson College, Washington, PA• Fall 2014
 Coinvestigated the effects of moisture levels on soil bacteria diversity at Abernathy Field Station
in Washington, PA
PRESENTATIONS
Poster Presentations
Bobnar, H., Emery E., Lindsey B., Noore J., Bostian P. Characterization of the K7M2 Tumor
Microenvironment in a Mouse Model. Washington and Jefferson College, PA. October 2016.
Oral Presentations
Bobnar, H., Emery E., Lindsey B., Noore J., Bostian P. Characterization of the K7M2 Tumor
Microenvironment in a Mouse Model. West Virginia University School of Medicine, WV. August 2016.

2. Harley Bobnar Curriculum Vitae, Page 2
COURSE HIGHLIGHTS
 General Biology I* and II*
 Organic Chemistry I* and II*
 Inorganic Chemistry*
 Analytical Chemistry*
 Physics I* and II*
 Calculus I
 Introduction to Chemical Research
 Cell Biology*
 Medicinal Chemistry
 Virology*
 Biochemistry*
 Bioorganic Chemistry
 Animal Physiology*
 Molecular Biology*
 Biophysical Chemistry*o
 Biochemistry Seminaro
*designates course accompanied by semester-long laboratory course o
Spring 2017
LABORATORY TECHNIQUES
Proficient in:
 Bacterial Culture Preparation
 Bacterial Genomic DNA Extraction
 Restriction Digest
 Agarose Gel Electrophoresis
 Polymerase Chain Reaction
 Bioinformatics Analysis (Mothur,
Ribosomal Database Project, Phylogeny.fr)
 Indirect Immunofluorescence Microscopy
 Culture of Eukaryotic Adherent Cells
 Culture of Frozen Cells
 Immunodiffusions (radial and double)
 Immunoblots (dot blot and Western blot)
 Protein Gel Electrophoresis
 Immunoelectrophoresis
 Restriction Digest
 Nuclear Magnetic Resonance Spectrum and
Analysis
 Thin Layer Chromatography
 Gas Chromatography
 Liquid Chromatography
 Cell Viability Assay
 Flow Cytometry
 Transformation of Plasmid Vectors into
Bacteria and Yeast
 Isolation of Plasmid from Bacteria and
Yeast Cultures
 Ligation of PCR Products into Plasmid
Vectors
Relevant Employment
 Organic Chemistry Laboratory Assistant, Fall 2015-Spring 2016
 Organic Chemistry, Teaching Assistant, Fall 2015
Academic Honors
 Merck Internships for Excellence in Science Funding Recipient, received funding for an off-
campus internship for Summer 2016
 Alpha Lambda Delta Honor Society, GPA above 3.50 and top 20% of class as freshman, 2014
 Dean’s List, GPA above 3.50, Fall 2013
 Scholar Select Scholarship Recipient
References furnished upon request