The document discusses Industrial Microbial Testing (IMT) and their quantitative real-time PCR (qRT-PCR) assays for testing microorganisms. IMT offers total bacteria count and total yeasts/molds count assays, as well as assays for pathogens like E. coli, Salmonella, C. botulinum, and others. The assays can be used to test various samples from food, pharmaceutical, aerospace and other industrial clients for applications like quality control, environmental monitoring, and product release testing.

1. Industrial Microbial Testing (IMT)
Andy Moreno
Vice President, Microbiological Sales Engineering
Industrial Microbial Testing (IMT)
Industrial Microbial Testing (IMT)
Cell: +559‐827‐8245
FAX: +866‐776‐3746
andy.moreno@imt‐pcr.com
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http://www.imt‐pcr.com
Linkedin Profile
Skype: Andy Moreno
TBC Facebook
May 19, 2010
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2. IMT qRT‐PCR Assays
• Total Bioburden Assays
– Total Bacteria Count Assay
– Total Yeasts & Molds Count Assay
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• Commercialized Pathogen Assays
– E. coli spp. Assay • Primers
– Salmonella spp. Assay
• Controls
– C. botulinum “E” A
C b t li “E” Assay
– Porcine Assay • Molecular Water
• R&D Pipeline
– Campylobacter Assay
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– Listeria spp. Assay
– Enterococcus spp. Assay
– Staphylococcus spp. Assay
– Pseudomonas spp. Assay
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– Xanthomonas spp. Assay
– Mycoplasma spp. Assay
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3. IMT’s Total Bacteria Count (TBC)*
an “Internal Investigation Tool”
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• Sample size: as desired by client
• Limit of detection: One (1) cell in 5 µl sample
– Determined by sample preparation
• Time to result‐‐collection to result: ~One (1) hour
• Sample run time:
Sample run time:
– Thermocycler #1: ~thirty (30) minutes
– Thermocycler #2: ~ninety (90) minutes
• I l i it All b t i ( k
Inclusivity: All bacteria (prokaryotes)
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• Exclusivity: Eukaryotic DNA (non‐bacterial)
*Patent Pending Slide 3 of 11

4. IMT’s Yeasts and Molds Assay*
an “Internal Investigation Tool”
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• Sample size: As desired by client
• Limit of detection: One (1) cell in 5 µl sample
– Determined by sample preparation
• Time to result‐‐collection to result: ~One (1) hour
• Sample run time:
Sample run time:
– Thermocycler #1: ~thirty (30) minutes
– Thermocycler #2: ~ninety (90) minutes
• Inclusivity: All yeasts & molds (eukaryotes)
Inclusivity: All yeasts & molds (eukaryotes)
• Exclusivity: Prokaryotes
*Patent Pending Slide 4 of 11

5. RT‐PCR Systems which can employ IMT products
Rotor‐Gene Q Roche LightCycler 2; LightCycler 480 ABI 7500 DxNA
GeneSTAT
Corbett Research
Corbett Research Eppendorf Cepheid
Rotor‐Gene 6000 SmartCycler
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6. IMT Product Lines by Industry
Pharma
Food Aerospace
QA/QC
Total Bioburden Assay
Total Bacteria Count Total Bacteria Count (TBA)
Yeasts & Molds Count NASA‐Boeing
Total Bacteria Count
Total Yeasts and Molds
Listeria
Salmonella
E. Coli
E C li
C. Bot. “e”
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7. Total Bioburden Assays: Pharma QA/QC
• Total Bacteria Count (TBC) and Total Yeasts and
Molds Count (TYMC) offer rapid results for Total
Molds Count (TYMC) offer rapid results for Total
Bioburden Assay.
• Benefit: Speed as compared to four (4) day culture
Benefit: Speed as compared to four (4) day culture
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8. Food: Positive Release Program
• Irrigation water tested
• Raw fruit and vegetables are washed and cleaned
• Peracetic Acid and Chlorine wash bath tested at the end of each run
• Fi i h d
Finished product is tested
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• Environmental samples collected in plant “hot spots” Slide 8 of 11

9. IMT’s Tests at an Aseptic Packer
• IMT’s Assay Implementation for DNA‐tested ‐HACCP
– Reverse Osmosis Water Testing
– Product in‐process testing
– Final Product Testing
– Yeasts and Molds Testing
easts a d o ds est g
– Shipment “Release” upon Final Product Testing report
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10. Current Industrial Clients
 Major Biopharma Company: USA
 Fresh Vegetable Packer: California, USA
 Aseptic Beverage Packer: California, USA
 Aerospace Agency QA/QC: California USA
 Biotechnology Equipment Manufacturer: USA
 Agricultural Agency—Water Testing: Mexico
 Food Preparation Factory: California, USA
 USDA, Ames, Iowa, USA
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Biotechnology Production Co., New Jersey, USA
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11. Questions & Answers
Questions & Answers
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