Scope of Industrial Microbiology and BiotechnologyDr. Pavan Kundur
Industrial microbiology defined as the study of the large-scale and profit motivated production of microorganisms or their products for direct use, or as inputs in the manufacture of other goods.
Scope of Industrial Microbiology and BiotechnologyDr. Pavan Kundur
Industrial microbiology defined as the study of the large-scale and profit motivated production of microorganisms or their products for direct use, or as inputs in the manufacture of other goods.
the presentation is about microbial endophytes, discovery of endophytes, their types, isolation methods of different types and identification and the useful impacts of them to the plant ecology.
secondary metabolites of plant by K. K. SAHU SirKAUSHAL SAHU
METABOLITES : Introduction . . .
The chemical compounds produced by plants are collectively called as phytochemicals.
Primary metabolites – participating in nutrition and metabolic processes inside the plant.
Secondary metabolites – those chemical compounds that do not participate in metabolism of plants but influencing the
ecological interactions between the plant and its environment.
The material describes components of industrial fermentation media with their respective metabolic importance for the industrial microbes. it also addresses industrial scale sterilization methods.
the presentation is about microbial endophytes, discovery of endophytes, their types, isolation methods of different types and identification and the useful impacts of them to the plant ecology.
secondary metabolites of plant by K. K. SAHU SirKAUSHAL SAHU
METABOLITES : Introduction . . .
The chemical compounds produced by plants are collectively called as phytochemicals.
Primary metabolites – participating in nutrition and metabolic processes inside the plant.
Secondary metabolites – those chemical compounds that do not participate in metabolism of plants but influencing the
ecological interactions between the plant and its environment.
The material describes components of industrial fermentation media with their respective metabolic importance for the industrial microbes. it also addresses industrial scale sterilization methods.
Microorganism and principle of biology,Medical microbiology and immunology,Soil microbiology,Industrial microbiology,Food microbiology,Water microbiology,Sewage microbiology
Role of Microorganism and Enzymes in Food Preservation by Pallavi Wani.pptxPallavi Wani
Food preservation?
Food preservation is a process in whichFruits and vegetables are prevented from getting spoilt. The color, taste, and nutritive values of food is also preserved. Food products lasts for a long period of time: shelf life of food product is increased
Role of Microorganism in Food preservation
As the human population is increasing, we need to adopt new techniques for producing qualitative and nutritious food. These microorganisms can be used to cope with the shortage of food supply.
Microorganisms are an important part of the food industry as these are helpful in food preservation and production.
Usually, microorganisms are used in making dairy products (yogurt and cheese), fermented vegetables (olives, pickles, and sauerkraut), fermented meats (salami), and sourdough bread.
These are also utilized for the production of wine and several other beverages.
Recently in the food industry, the use of microorganisms has started on a large scale for the production of chocolate, food color, from preserving fruits, vegetables and meat, and as probiotics which are helpful for human health.
Microorganisms contribute to the smell, texture and taste of the food
For example, bacteria are Lactobacilli that is used for the production of food as these bacteria ferment lactic acid
Lactobacilli are conventionally used to add aroma and texture to the food while preventing the spoilage of dairy products, meat and vegetables as well as silage.
Microorganisms that are present in the GI tract are known for producing health promoting compounds that are called probiotics
These probiotics in fermented products help in the preservation of milk products through the formation of lactic acid which adds to the flavor as well as nutritional value of the food
The role of different types of microorganisms in various food processes
SI.No Microbial Activity
Microbes
Use in industry
1 Pectinolytic activity Lactobacillus brevis ,Erwinia herbicola
Cofee industry
2 Naringinase activity Aspergillus niger,
Aspergillus oryzae,
Fruit juices industry
3 Fermentation Streptococcus thermophilus and Lactobacillus bulgaricus Dairy industry
4 Protease activity Bacillus tequilensis Brewing industry
5 Asparaginase activity
Cladosporium sp Baking industry
1. Role of Lactic Acid Bacteria
Lactic acid bacteria are being used in a number of food production and storage methods in the modern food industries.
Lactobacilli are commonly used for the storage of uncooked fermented sausages and sliced meats to avoid pathogenic
These bacteria have replaced the chemical additives such as sodium lactate and potassium acetate which were used for the safety and quality
Mechanism- of action of lactic acid bacteria in raw fermented sausages is the conversion of sugars to lactic acid through fermentation. This contributes to the unfavorable conditions for the growth of pathogenic and spoilage microorganism.
Lactobacillus spp.
Industrially important microbes their large scale productionVibhaKumari13
The above presentation is useful for the Students who want to gain and enrich their knowledge about the large scale production of industrially important microbes and fermentation procedure.
This will mainly be helpful for Students opting Agricultural microbiology
Use of microbes in industry. Production of enzymes-General consideration-Amyl...Steffi Thomas
Industrial uses of microbes, properties of useful industrial microbes, various industrial products, production of enzymes-general consideration-amylase, catalase, peroxidase, lipase, protease, penicillinase, procedure for culturing bacteria and inoculum preparation, submerged fermentation and solid state fermentation, uses of different enzymes
Major Microorganisms Utilized In The Food And Beverage Sector.pdfFoodresearchLab
Major Microorganisms Utilized In the Food and Beverage Sector:
In the food sector, microorganisms play a significant role and help to solve the challenges in the food and beverage industry. They’re utilized to create a variety of foods, and they’re also to blame for food rotting, which leads to poisoning and sickness. Microbial contamination of food items occurs most commonly on the journey from the farm to the processing facility, during processing, storage, transportation, distribution, and before consumption.
Prefaces:
1. What are the Microorganisms and their Applications in the Food Industry
2. What are the Factors affecting Microorganisms Growth
What food Research lab offers you?
Food Research Lab comprises a team of food microbiologists, food technologists, formulation scientists, food product developers and culinary experts who can develop your food products and conduct several tests for launch in the market. The shelf life of the products can be enhanced using microbial study, and the organoleptic properties can be amplified.
Want to know what the foods that contain microorganisms are: https://bit.ly/3L3zD7e
Need an experienced food scientist team and culinary experts and nutritionists formulate food and drink products:
Contact us:
Website: https://www.foodresearchlab.com/
Contact no: UK- +44- 161 818 4656, INDIA- +91 9566299022
Email: info@foodresearchlab.com
Gains due to bacteria, Food processing,Biotechnology
Genetic engineering
Fibre retting
Pest control
Bioremediation
Digestion
Tanning Of Leather
Medicines.
Industrial microbiology is a branch of applied microbiology in which microorganisms are used in industrial processes; for example, in the production of high-value products such as drugs, chemicals, fuels and electricity.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
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Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
2. Industrial microbiology may be defined as the study of
the large-scale And profit motivated production of micro
organism or their products For direct use or as inputs in
the manufacture of other good.
Thus yeasts may be produced for direct consumption as
food for human Or as animal feed, or for using in the
bread making.
And also used in pharmaceutical industry,
bevarage industry,Cosmetic industry,
agricultural industry.
3. Bacteria
These include chemicals manufacturing such
as ethanol, Acetone, organic acid, enzymes and
perfumes .
Bacteria are most important in the production
of pharmaceuticals
4. The most important bacteria in food
manufacturing lactobacillus species
also referred to as lactic bacteria.
Application
Dairy industry,
Meat industry,lactic bacteria develop
the flavor and color of the product.
Wine industry
5.
6. Teixobactin was isolated from a newly
identified gram positive bacteria.
Can kill species including methicillin –
resistant staphylococcus
oureus(MRSA) and mycobacterium.
Bacteria in bioremediation
Microbial bioremediation is the
use of prokaryotes to remove
pollutants.
7.
8. Many fungi are useful to humans and
have been exploited both industrially
and commercially.
Yeast and other fungi play a critical role
in drug production , food processing,
bio control agents , enzyme
biotechnology , as well as research and
development.
9. Saccharomyces cerevisiae presence of
exess glucose represses respiration.
In principle ,materials rich in sugars (or
starch)are then fermented resulting in
the production of alcohol.
Mainly three products
Beer
Wine
Sake
10. Fungi are responsible for a range of
favours including terpenes, menthol,
lactones.
Fungi produces a range of compounds
that alter the color of food.
Ex; monoascus purpurecis has been
traditionally used for the production of
red wine
11. Play a important role in the fertility of soil
Protozoa role in mineralizing nutrients,
making them available for use by plants
and other soil organisms.
Ex; protozoans are dinoflagellates,
amoebas, paramecia,and plasmodium.
Protozoan play important roles in waste
water treatment.
12. Algae are used in wastewater treatment.
Reducing the toxic chemicals.
Algae can be used to capture fertilizers
in runoff from farms.
Algae can be used as fertilizer.
13. Spirulina
Human and animal food or
nutritional supplement.
Also know as: blue green algae
Astaxanthain
Alzheimer's disease
Parkinson's disease
Micro algae also used in
cosmetic products