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1. EVALUATION OF PRELIMINARY PHYTOCHEMICAL and INVITRO ANTIOXIDANT ACTIVITY OF
Homalomena aromatica (tuber)
Madhusmita Kumari
Under the guidance of
Mr. Ramen Kalita,M Pharm,(Guide)
Assistant Professor ,Faculty of
Pharmaceutical Science,ADTU
FACULTY OF PHARMACEUTICAL SCIENCES
ASSAM DOWN TOWN UNIVERSITY ,GUWAHATI-781026
ASSAM
Submitted by
Mpharm 2nd semester(pharmaceutics)

2. CONTENT
1. INTRODUCTION
2. LITERATURE REVIEW
3. AIM AND OBJECTIVES
4. METHODOLOGY
5. RESULT & DISCUSSION
6. CONCLUSION
7. REFERENCE

3. INTRODUCTION
Homalomena aromatica(family-araceae) is commonly known as
sugandhmantri which contains essential oils are known for high purity and
have a mesmerizing aroma that makes them widely used in aromatherapy
applications. Thymol, pinene, methyl anthranilate, indole, benzyl alcohol,
anti inflammatory, anti spasmodic, antidepressants assists in medications, for
spiritual healing, calming the nerves.It is used for immune support as well as
for treating cold,infection,injury or emotional stress.It is most available in
assam, arunachal pradesh, nagaland,mizoram,manipur,tripura of india.

4.  Homalomena aromatica is a perennial,
aromatic and rhizomatous herb.the tuber is
covered with dark brown leafy scales and
mature rhizome is sometimes curved and
running over ground. Numerous white
roots were borne on every part of the
rhizome and the stem is short and slow
growing.the leaves are radical,lucid,26-
27cm*10-12cm,acuminate;base cordate or
sagittate;lobes rounded;petiole up to 100cm
long;spadix sub-cylindric;spathe pale
green-yellow,erect or pendant;flower
arising form the axils and centre of he
leaves on the green scape;berries
white,oblong,1-seeded.
 Flowering/fruiting time: june-september

5. Taxonomic classification
Common names of homalomena aromatica are-
 Gansena-assamese
 Sugandhmantri-hindi
 Gandhakochu-bengali
 Aancheeri-mizoram
 Gondhoi-manipur
KINGDOM: Plantae
SUBKINGDOM: angiosperms
CLASS: monocots
ORDER: alismatales
FAMILY: araceae
SUBFAMILY: Aroideae
TRIBE: homalomeneae
GENUS: Homalomena
SPECIES: aromatica

6. LITERATURE REVIEW
1.Ashish kumar goswami,zartaj washmin banu,hemanta kr sharma, have studied on
the rhizome of Homalomena aromatica Schott(family araceae) and found that linalool
is the chief constituent of the essential oil portion of rhizome of homalomena aromatica
is a monoterpene alcohol having tremendous effect on the central nervous system.
2. Policegoudra RS,Goswami S,Aradhya SM,have studied on the bioactive
constituents of rhizome of homalomena aromatica and found that its essential
oil has antifungal activities against dermatophytes and yeasts.
3. Chandana CB,anindita T,Anindhya SD,Acheenta GB have studied on the
ethanolic extract of Homalomena aromatica and found that it has ulcer protective
activity by using HCL-ethanol,cold restraint stress and indomethacin induced
ulcer models in wistar rat.
4. Chandana choudhury barua,Anindita Talukdar,Bhargavi phukan have studied
on ethanolic extract of homalomena aromatica(araceae) root and found its
antioxidant activity.ethanolic extract of homalomena aromatica root was selected
to evaluate their in vitro antioxidant potential.

7. AIM: EVALUATION OF PRELIMINARY PHYTOCHEMICAL AND
INVITRO ANTIOXIDANT ACTIVITY OF HOMALOMENA
AROMATICA (TUBER)
OBJECTIVES:-
COLLECTION OF THE PLANT
AUTHENTICATION OF THE PLANT
EXTRACTION OF THE MATERIALS
PHYTOCHEMICAL SCREENING OF THE TUBER EXTRACT
ANTIOXIDANT ACTIVITY OF THE ALCOHOLIC TUBER EXTRACT

8. COLLECTION
AUTHENTICATION
DRYING AND GRINDING OF THE TUBER
EXTRACTION BY SOXHLET
PHYTOCHEMICAL SCREENING
ANTIOXIDANT ACTIVITY

9.  Homalomena aromatica is commonly available in north east india,but mainly it was
collected from mirza,a small village of the district kamrup,assam. It was available in
the form of mans cultivation process.we have collected it in the month of
september.the tuber or rhizome is our main part for the chemical constituent.we have
also collected the plant for the authentication purpose along with the flowers
available.
Shade drying
The tuber of the plant Homalomena aromatica was collected and allowed for shade
drying for near about 20 days. After complete drying of the tuber then they are
allowed for grinding process into small powdered form.

10. Authentication of the plant
 The plant Homalomena aromatica
was identified by the department of
botany,guwahati university by Dr.
N.Devi
Sl
no
Name of the species family Collection
no.
Accessi
on no.
Collectio
n date
1 Homalomena
aromatica(Spreng.)
Schott
Araceae 674 18491 03-09-
2018
Herbarium prepared
The herbarium was prepared in a
proper herbarium sheet with all the
necessry parts required for its
identification purpose . The plant
was completely dried without any
fungus formation.

12. EXRACTION BY SOXHLET APPARATUS
The extraction was done by taking two solvent one is ethanol and the other
by water. 250gm of the dried materials were taken in each soxhlet and then
the extraction was done for 3 days. The extract was found to be 70gm.

13. RESULT & DISCUSSION
TESTS RESULTS
Mayer's test positive
Wagners test positive
Dragendroffs test positive
DETECTION OF ALKALOID:-
DETECTION OF CARBOHYDRATE:-
PHYTOCHEMICAL SCREENING:- The extracts were subjected to
phytochemical analysis and the results are represented in the tables below:-
TESTS RESULTS
Molish’s test positive
Fehling’s test positive
Benedict test positive
Barfoed’s test positive

14. DETECTION OF SAPONINS:-
TESTS RESULTS
Test for detection of saponins positive
DETECTION OF PHENOLIC COMPOUNDS:-
TESTS RESULTS
Ferric chloride test positive
Gelatin test positive
DETECTION OF GLYCOSIDES:-
TESTS RESULTS
Lead acetate test positive
Keller-killiani test positive

15. DETECTION OF PROTEINS & AMINO ACIDS:-
TESTS RESULTS
Millon’s test negative
Biuret test negative
Ninhydrin test negative
Legals test negative
DETECTION OF FLAVANOIDS:-
RESULTS positive
PHYSICAL PARAMETERS OF EXTRACT:-
Homalomena
aromatica
COLOUR BLACK
Extract 70gm

16. TESTS RESULTS
Spot test positive
Saponification test positive
DETECTION OF FIXED OILS & FATS:-

17. DETERMINATION OF ANTIOXIDANT ACTIVITY
SL NO CONCENTR
ATION
(µg/ml)
INHIBITION %
1 20 31.39 ±0.91
2 40 44.13 ±0.93
3 60 56.21 ±0.25
4 80 62.3 ±0.32
5 100 71.02 ±0.11
All data were expressed in ± SEM

18. 0
10
20
30
40
50
60
70
80
90
0 20 40 60 80 100 120
percentageinhibition
CONCENTRATION (µg/ml)
I%
Y=0.811
R2=0.449
I %
Linear (I %)
Determination of IC50
SL NO EXTRACT IC50(µg/ml)
1 METHANOL 61.65±01.12
2 ASCORBIC ACID 11.36±1.02

19. CONCLUSION
 After performing the experiment we found that
alkaloids,carbohydrate,saponin,glycoside,phenolic
compounds,flavanoids,fixed oils and fats were
present in tuber of the plant. And our extract also
have produced a very good DPPH radical
scavenging activity due to the presence of
different phytochemical constituents and this plant
may be useful for further investigation.

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