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Histology of Bone Tissue
Histology of Bone Tissue
• It contains an abundant extracellular matrix that
surrounds widely separated cells
• The extracellular matrix contains:
– 15 % water
– 30 % collagen fibers
– 55 % crystallized mineral salts
Histology of Bone Tissue
• Four types of cells are present in bone tissue:
– Osteogenic cells
– Osteoblasts
– Osteocytes
– Osteoclasts
Cells in Bone Tissue
Osteogenic cells
• They are bone stem cells
• The only bone cells to undergo cell division
• They form osteoblasts
• They are found in:
– Inner portion of the periosteum
– Endosteum
– Canals within bone that contain blood vessels
Cells in Bone Tissue
Osteoblasts
• They are bone-building cells
• Functions:
– Synthesis of collagen fibers and other organic
components needed to build ECM
– Initiating calcification
• As they surround themselves with ECM, they
become osteocytes
Cells in Bone Tissue
Osteocytes
• Mature bone cells
• Their function is to maintain bone tissue’s
metabolism
• Like osteoblasts, osteocytes do not undergo
cell division
Cells in Bone Tissue
Osteoclasts
• Huge cells derived from the fusion
of as many as 50 monocytes
• They are concentrated in the
endosteum
Cells in Bone Tissue
Osteoclasts
• Ruffled border:
– Deeply folded plasma membrane
– Here the cell releases powerful lysosomal enzymes
and acids that results in resorption
• Help regulate blood calcium level in response to
certain hormones
• They are also the target cells for drug therapy used to
treat osteoporosis
Cells in Bone Tissue
Osteoclasts
• Resorption
– Digestion of protein and mineral components of
the ECM of bone
– It is part of the normal development, growth,
maintenance, and repair of bone
Compact Bone Tissue
• Also referred to as cortical or dense bone
• It is a type of bone tissue observed at the surface of a
bone
• It makes up the bulk of the diaphyses of long bones
• It is porous, with an abundance of microscopic spaces
and canals
• It provides protection and support and resists the
stresses produced by weight and movement
Compact Bone Tissue
Osteons/haversian systems
• Repeating structural units that composes of
compact bone
• Each osteon consists of concentric lamellae
arranged around a central (haversian) canal.
Compact Bone Tissue
Osteons/haversian systems
• Concentric lamellae
– Circular plates of mineralized ECM of increasing
diameter
– Surrounds a small network of blood vessels,
lymphatics, and nerves located in the central canal
• Lacunae
– Small spaces between the concentric lamellae
– They contain osteocytes
Compact Bone Tissue
Osteons/haversian systems
• Canaliculi
– Tiny small channels radiating in all directions from
the lacunae
– They are filled with extracellular fluid
– Slender fingerlike processes of osteocytes are
inside it
Compact Bone Tissue
Osteons/haversian systems
• Canaliculi
– Connects lacunae with one another and with the
central canals
• Forming miniature system of interconnected canals
throughout the bone
– This system provides blood circulation to the
osteocytes
Compact Bone Tissue
Osteons/haversian systems
• Osteons of long bone are aligned in the
direction parallel to the length of the diaphysis
– Long bone resists bending or fracturing even when
considerable force is applied from either end
Compact Bone Tissue
Osteons/haversian systems
• Interstitial lamellae
– The lamellae that are contained in the areas
between neighboring osteons
– It also have lacunae with osteocytes and canaliculi
– They are fragments of older osteons that have
been partially destroyed during bone growth
Compact Bone Tissue
Osteons/haversian systems
• Perforating canals/Volkmann’s canals
– Transverse canals through which neuro-vasculatures
from the periosteum penetrate the compact bone
– Their neuro-vasculatures connect with those of the
medullary cavity, periosteum and central canals.
Compact Bone Tissue
Osteons/haversian systems
• Circumferential lamellae
– Lamellae that are arranged around the entire
outer and inner circumference of the shaft of a
long bone
Compact Bone Tissue
Osteons/haversian systems
• Outer circumferential lamellae
– Circumferential lamellae directly deep to the
periosteum
– They are connected to the periosteum by Sharpey’s
fibers
• Inner circumferential lamellae
– Circumferential lamellae that line the medullary cavity
Spongy Bone Tissue
• Also referred to as trabecular or
cancellous bone tissue
• They does not contain osteons
• It is always covered by a layer of
compact bone for protection
• It consists of trabeculae
Spongy Bone Tissue
• Trabeculae
– Are lamellae that are arranged in an irregular
pattern in the spongy bone
– Between them there are spaces that are filled with
red bone marrow /yellow bone marrow
– Each of them consists of concentric lamellae,
osteocytes and canaliculi
Spongy Bone Tissue
• Makes up the interior bone tissue of
short, flat, sesamoid and irregular
bones
• In long bones it forms
– Core of the epiphyses
– Narrow rim bordering the medullary
cavity of the diaphysis
Spongy Bone Tissue
• Spongy bone is different from compact bone
in two respects.
1. Spongy bone tissue is light
▪ Allows the bone to move more readily when pulled by a
skeletal muscle
2. Their trabeculae support and protect the red
bone marrow.

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Histology of Bone.pptx.pdf

  • 2. Histology of Bone Tissue • It contains an abundant extracellular matrix that surrounds widely separated cells • The extracellular matrix contains: – 15 % water – 30 % collagen fibers – 55 % crystallized mineral salts
  • 3. Histology of Bone Tissue • Four types of cells are present in bone tissue: – Osteogenic cells – Osteoblasts – Osteocytes – Osteoclasts
  • 4. Cells in Bone Tissue Osteogenic cells • They are bone stem cells • The only bone cells to undergo cell division • They form osteoblasts • They are found in: – Inner portion of the periosteum – Endosteum – Canals within bone that contain blood vessels
  • 5. Cells in Bone Tissue Osteoblasts • They are bone-building cells • Functions: – Synthesis of collagen fibers and other organic components needed to build ECM – Initiating calcification • As they surround themselves with ECM, they become osteocytes
  • 6. Cells in Bone Tissue Osteocytes • Mature bone cells • Their function is to maintain bone tissue’s metabolism • Like osteoblasts, osteocytes do not undergo cell division
  • 7. Cells in Bone Tissue Osteoclasts • Huge cells derived from the fusion of as many as 50 monocytes • They are concentrated in the endosteum
  • 8. Cells in Bone Tissue Osteoclasts • Ruffled border: – Deeply folded plasma membrane – Here the cell releases powerful lysosomal enzymes and acids that results in resorption • Help regulate blood calcium level in response to certain hormones • They are also the target cells for drug therapy used to treat osteoporosis
  • 9. Cells in Bone Tissue Osteoclasts • Resorption – Digestion of protein and mineral components of the ECM of bone – It is part of the normal development, growth, maintenance, and repair of bone
  • 10. Compact Bone Tissue • Also referred to as cortical or dense bone • It is a type of bone tissue observed at the surface of a bone • It makes up the bulk of the diaphyses of long bones • It is porous, with an abundance of microscopic spaces and canals • It provides protection and support and resists the stresses produced by weight and movement
  • 11.
  • 12. Compact Bone Tissue Osteons/haversian systems • Repeating structural units that composes of compact bone • Each osteon consists of concentric lamellae arranged around a central (haversian) canal.
  • 13. Compact Bone Tissue Osteons/haversian systems • Concentric lamellae – Circular plates of mineralized ECM of increasing diameter – Surrounds a small network of blood vessels, lymphatics, and nerves located in the central canal • Lacunae – Small spaces between the concentric lamellae – They contain osteocytes
  • 14. Compact Bone Tissue Osteons/haversian systems • Canaliculi – Tiny small channels radiating in all directions from the lacunae – They are filled with extracellular fluid – Slender fingerlike processes of osteocytes are inside it
  • 15. Compact Bone Tissue Osteons/haversian systems • Canaliculi – Connects lacunae with one another and with the central canals • Forming miniature system of interconnected canals throughout the bone – This system provides blood circulation to the osteocytes
  • 16. Compact Bone Tissue Osteons/haversian systems • Osteons of long bone are aligned in the direction parallel to the length of the diaphysis – Long bone resists bending or fracturing even when considerable force is applied from either end
  • 17. Compact Bone Tissue Osteons/haversian systems • Interstitial lamellae – The lamellae that are contained in the areas between neighboring osteons – It also have lacunae with osteocytes and canaliculi – They are fragments of older osteons that have been partially destroyed during bone growth
  • 18. Compact Bone Tissue Osteons/haversian systems • Perforating canals/Volkmann’s canals – Transverse canals through which neuro-vasculatures from the periosteum penetrate the compact bone – Their neuro-vasculatures connect with those of the medullary cavity, periosteum and central canals.
  • 19. Compact Bone Tissue Osteons/haversian systems • Circumferential lamellae – Lamellae that are arranged around the entire outer and inner circumference of the shaft of a long bone
  • 20. Compact Bone Tissue Osteons/haversian systems • Outer circumferential lamellae – Circumferential lamellae directly deep to the periosteum – They are connected to the periosteum by Sharpey’s fibers • Inner circumferential lamellae – Circumferential lamellae that line the medullary cavity
  • 21. Spongy Bone Tissue • Also referred to as trabecular or cancellous bone tissue • They does not contain osteons • It is always covered by a layer of compact bone for protection • It consists of trabeculae
  • 22. Spongy Bone Tissue • Trabeculae – Are lamellae that are arranged in an irregular pattern in the spongy bone – Between them there are spaces that are filled with red bone marrow /yellow bone marrow – Each of them consists of concentric lamellae, osteocytes and canaliculi
  • 23. Spongy Bone Tissue • Makes up the interior bone tissue of short, flat, sesamoid and irregular bones • In long bones it forms – Core of the epiphyses – Narrow rim bordering the medullary cavity of the diaphysis
  • 24. Spongy Bone Tissue • Spongy bone is different from compact bone in two respects. 1. Spongy bone tissue is light ▪ Allows the bone to move more readily when pulled by a skeletal muscle 2. Their trabeculae support and protect the red bone marrow.