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THE INSIDE LOOK OF
 BACTERIOPHAGES
  LYTIC AND LYSOGENIC CYCLES OF
          BACTERIOPHAGE




 Nicole Colón, Astrid Díaz, Lysander Borrero y Alberto
 Cintrón
OBJECTIVES:

• Explain what mycobacteriophages are and why
  are they important.



• Distinguish between bacteriophage lytic and
  lysogenic cycles.
MYCOBACTERIOPHAGES

• Viruses that infect bacteria belonging to the
  mycobacteria genus.
• Can be found in the environment, including soil.
• Can cause diseases
• Are used as a model to study biological process




                           Institute for Molecular Virology. University of
                           Wisconsin . 2005
BACTERIOPHAGE STRUCTURE



              DNA
                       Head




                      Tail
LIFE STYLES

• Mycobactheriophage can be group according to
  their life styles.

• Two different life cycle:
 a) Lytic cycle
 b) Lysogenic cycle
Lytic Cycle

 Used by virulent phages

 Is characterized by phage
reproduction followed by host
cell lysis.
LYSOGENIC CYCLE
LIFE CYCLE VIDEO

• http://highered.mcgraw-
  hill.com/sites/0072556781/student_view0/chapter17
  /animation_quiz_2.html
REFERENCES

• Rubin M.2012.Cluster Classification of
  Mycobacteriophages Isolated From Tropical Soils of
  Puerto Rico.
• Prescott.Harley.Klein.2005.Microbiology. 6th edition.
  McGraw-Hill Higher Education.
• About Biology. Regina Bailey. (unknown).
  Bacteriophages.
ACKNOWLEDGMENTS

•   Dr. Rubin
•   TA: Melisa Medina
•   TA: Valeria Rivera
•   RISE Program
THANKS FOR YOUR ATTENTION !
QUESTIONS ?
Table 1
Figure 1
Table 2
 AyB

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Group # 1 Mycobacteriophages Presentation

Editor's Notes

  1. It can cause Mycobacterium tuberculosis, bacteria that cause lung disease.Mycobacterium leprosy cause leprosy
  2. The tail is where the DNA is injected to the bacteria
  3. Life cycles are related to the ways that they can infect bacteria and reproduce.
  4. Infect the host by attaching to the outer membrane of the bacterial cell. 2) the genetic material is injected into the bacteria Degradation of the host bacterial chromosomal DNA ( eliminate competing instructions from the bacterial genome) Phage replicates 5) phage assembly: the phage genomic DNA is packaged within the mature protein coat6) Bacterial cell lysis
  5. Used by temperate phage 2) characterized by phage integration into the bacterial chromosome.Steps: 1) the genetic is injected into the bacteria 2) the entering phage genome is confronted with a decision to determine which of two pathways to follow.3) If the environment conditions are favorable integration occurs. ( the phage inserts its genome into the host chromosome). After this genome is replicated and each dividing cell receive a copy.4) Unfavorable conditions cause excision ( phage genome exit the host chromosome) followed by the lytic cycle.