Covers syllabus, assesment parameters, what to expect from the course

1. Course Overview
Mr. Veerendra Singh Nagoria
Assistant Professor Biotechnology,
School of Life Science, Rai University Ahmedabad
Veerendra.nagoria@gmail.com, veerendra@outlook.com

2.  Minimum Attendance requirement to sit for final
semester examination is 75%
 No Assignment after defined time limit
 Open to discussion on Web over Google group.
 PPTs can be taken from my Slideshare account
but it is limited in nature consult books
 Participation during lecture session will be
having additional weightage

3. CONTINUOUS EVALUATION 30 Marks
Mid-Term Assessment As per examination
Assignment 1 10 Marks
Viva on Assignment 1 5 Marks
Assignment 2 10 Marks
Viva on Assignment 2 5 Marks
Class Presentation 15 Marks
Class Participation 5 Marks
END SEMESTER EVALUATION 70 Marks

4.  Introduction to concepts, Definitions,
Discoveries
 Overview of fundamental processes:
Replication, Transcription, Translation
 Basic Methodology
 Restriction modification system and
Restriction Enzymes
 DNA modification enzymes: Nucleases,
Polymerases, Enzymes modifying the ends of
DNA molecule

5.  Vectors: Plasmids, Bacteriophages, Cosmid,
Phagemid, Artificial chromosomes
 Host cell types- prokaryotic and eukaryotic
 Transfer of vector into host cell:
Transformation, Transfection, Packaging of
phage DNA, Alternative DNA delivery
methods

6.  Cloning from mRNA, Genomic DNA
 Cloning after restriction digestion; blunt and
cohesive
 Creation of cloning site by PCR
 Cloning using Linkers and Adapters
 Cloning by recombination; GATEWAY
technology
 TA Cloning, TOPO Cloning, Ligation free
cloning

7.  Screening methods; Chromogenic substance,
Insertional inactivation, Complementation of
defined mutation
 Selecting recombinant clones; Reporter
gene, elimination of non-transformed cells,
identification of clones having Recombinant
DNA
 Sequence Dependent screening; Colony
hybridization, Chromosome walking,
Screening by PCR, Gene Tagging

8.  Genetic Engineering & Biotechnology;
protein production, protein engineering,
scale up
 Medicinal & Forensic Application of Gene
Manipulation; Diagnosis, Treatment and
DNA profiling